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- 저자 : 최은화 (검색결과 264 건)
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개 혈청화학검사항목의 장기간의 개체 내 변이와 critical difference
최은화,신일섭,방동하,김유석,황철용,윤화영,이창우 한국임상수의학회 2005 한국임상수의학회지 Vol.22 No.3
The purpose of this study was to assess intra-individual variations of clinicochemical parameters and calculate critical differences in healthy dogs during long-term periods. To calculate the critical difference of clinicochemical parameters, blood samples from 20 apparently clinically healthy dogs were collected once weekly for eight consecutive weeks. The critical difference was calculated as 9.01mg/dl for urea, 0.52mg/dl for creatinine, 0.99g/dl for total protein, 0.39g/dl for albumin, and 20.64mg/dl for glucose. If two consecutive results differ by less than the critical difference value, it can be concluded that the difference is probably due to physiological variation. However, when the difference is greater than the critical value, other factors, either related to progression of the disease or the presence of concurrent disease, are more likely to be involved.
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First Record of Maritime Pseudoscorpion Garypus japonicus (Garypidae) from Korea
최은화,황의욱 한국동물분류학회 2009 Animal Systematics, Evolution and Diversity Vol.25 No.3
A maritime pseudoscorpion Garypus japonicus Beier, 1952 collected from Dokdo Island in the East Sea of Korea is reported and illustrated, which is the first record in Korea. The family Garypidae is also newly recorded in Korea. G. japonicus has been known only from Japan by Beier (1952) so far. Through the present study, its distributional range is extended to Korea.
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최은화,고지호 대한초음파의학회 2008 ULTRASONOGRAPHY Vol.27 No.2
혈관육종은 드문 종양이다. 특히 혈관육종이 낭종성 변화를 보여 후복막에 생긴 경우 다른 후복 막의 낭종성 종양과 감별이 어렵다. 이에 저자들은 복통을 주소로 내원한 61세 여자 환자에서 후 복막에 발생하여 낭종성 변화를 보인 혈관육종을 경험하였기에 보고하고자 한다. 시행한 초음파와 CT 에서 불규칙한 격막과 벽의 비후가 있는 다방성 낭성종괴가 후복막에서 관찰되었고, 조영증강 CT 에서 낭성종괴의 불규칙한 벽이 조영증강되었다. 종괴는 적출술 후 조직검사에서 혈관육종으로 확진되었다. A retroperitoneal angiosarcoma is rare. Especially, a cystic change in retroperitoneal angiosarcoma is extremely rare and it is difficult to distinguish an angisarcoma with a cystic change in the differential diagnosis with other retroperitoneal cystic masses. We report here a case of a cystic change in a retroperitoneal angiosarcoma of a 61-year-old woman who complained of abdominal pain. Imaging studies, including ultrasonography and computed tomography, detected a lobulating cystic mass with inner enhancing irregular septations and walls in the retroperitoneum. The tumor was surgically resected and was histopathologically diagnosed as an angiosarcoma.
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소아에서 원인불명열의 진단적 접근 - 감염성 질환을 위주로 하여-
최은화 대한소아청소년과학회 2007 Clinical and Experimental Pediatrics (CEP) Vol.50 No.2
Fever of unknown origin (FUO) has been a convenient term used to classify patients who warrant a particular systemic approach to diagnostic evaluation and management. The greatest clinical concern in evaluating FUO is identifying patients whose fever has a serious or life-threatening cause when a delay in diagnosis could jeopardize successful intervention. Thorough history and complete physical examination are critical to uncover the etiologic diagnosis. Most cases of FUO in children are caused by atypical presentations of common diseases rather than by typical manifestations of rare disorders. Selection of diagnostic tests and speed of investigation should be guided by a knowledge of the disease severity, patient age, epidemiologic and geographic information, and any positive findings from a detailed history and physical examination. The three most common causes of FUO in children are infectious diseases, connective tissue diseases, and malignancy. In general, the prognosis of FUO in children is better than that of adults. Although the outcome is dependent on the primary disease process, fever abates spontaneously in most cases in whom the cause of fever remains unclear.
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E . coli 에서 사람 프리트롬빈 2 cDNA 의 크로닝과 발현
최은화,김용주,김종명,홍효정,한문희,김지영 ( Eun Hwa Choi,Yong Joo Kim,Jong Myoung Kim,Hyo Jeong Hong,Moon Hi Han,Ji Young Kim ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.2
A 0.6 kbp of 3`-portion of human prothrombin cDNA was isolated from a liver cDNA library of λgt 10 by in situ plaque hybridization. A cDNA fragment corresponding to the N-terminal region of prethrombin 2 was obtained from another cDNA library synthesized by reverse transcription of human liver mRNA using a specific oligonucleotide as a primer. The 48 bp cDNA sequences starting from the first nucleotide to the AvaI site of human prethrombin 2 cDNA was chemically synthesized such that prethrombin 2 can be expressed directly in E. coli expression vector system. The open reading frame(ORF) for human prethrombin 2 was cloned in pUC18 by combining the synthetic fragment and the cDNA fragments. Expression plasmids were constructed in which the ORF for human prethrombin 2 was inserted into the site downstream from the thermoinducible leftward promoter (P_L) and the cII ribosome binding site of bacteriophage λ. Upon temperature induction, the plasmids expressed 34,000 dalton polypeptide, which was accumulated to an amount corresponding to 5-10% of the total bacterial proteins. The 34,000 dalton polypeptide was identified as human prethrombin 2 by SDS-gel electrophoresis and Western blot analysis.
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