축우 부루셀라병의 ELISA 진단법에 관한 연구

임윤규,이두식,박전홍,양기천,김승호,김공식,현관종,김우택,이영순 濟州大學校 農科大學 動物科學硏究所 1993 動物科學論叢 Vol.8 No.1

Enzyme-linked Immuno sorbent Assay (ELISA) for the serological diagnosis oi Bruceiia abortus was developed and compared with plate aggluhnation test Cell wall antigen was extracted from Brucella abortus 1119-3 by sonicabon and with a sodium deoxychlate solution Optimum protein concentra tion of coating antigen 0.4㎍/100㎕ protein on each microtiter plate well. Horse radish peroxidase(HRP) labled protein-G was used as a tracer of reacted antibodies ELISA confirmed the agreeable results of 40 rases out of 43 cases by plate aggulutination test ELISA diagnosed positive cases (10 out of 12) and negatiw cases (1 out of 12) with dubious sera by plate agglutination test From this results EL ISA could be used for the early diagnostic tools of Brucellosis in cattle.

염화수소(Hydrogen Chloride)와 관련성이 추정되는 직업성 천식 1례

임현술,유선희,김두희,이영현 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

염화수소(hydrogen chloride)는 분자식은 HCl이며 염산가스라고 불리기도 하며 강한 자극성 냄새가 나는 무색의 기체이다. 물에 녹아서 염산이 되며 공기 중에서 물에 녹기 쉬운 성질 때문에 연무 형태로 존재한다. 본 증례로 34세 남자로 타이어에 사용되는 철사를 제조하는 공장에 다니는 근로자로 간헐적으로 고농도의 염화수소에 폭로된 후 1년 8개월의 잠복기를 거친 후 기관지 천식이 발생한 예로 총 호산구수는 증가되어 있었으나 혈청 IgE는 정상 범위였다. 55가지 알레르기항원을 사용한 피부 단자검사에서 House dust, Hay dust, Mixed grain, Dermatophagoides farinae와 Dermatophagoides pterynyssinus에 양성 반응을 보였고, 유발검사에서는 양성 반응을 보였다. 직업성 여부를 파악하기 위해 작업장 내에서 8시간 동안 근무하면서 30분 내지 한시간 간격으로 폐기능검사를 시행한 결과 1초간 노력성 호기량(FEV1) 이 20%이상 감소하여 2시간 동안 지속되는 양성 반응을 관찰할 수 있었다. 본 증례는 외인성 천식 소질이 있으면서 작업장 내에서 고농도의 염산증기에 폭로되어 기관지 천식 발작이 되었는데, 작업장의 고농도의 염화수소가 기관지 천식의 기여요인 또는 악화요인으로 작용하였다고 생각한다. Occupational asthma is an importent occupation-related disease. We exprienced a case of asthma induced by high concentration of hydrogen chloride vapour. The prevalence rate of this asthma is unknown. The patient of the case complained of cough, sputum and dyspnea after exposure to hydrogen chloride. Serum IgE(IgE-PRIST) was normal range but total eosinophil count was elevated. FEV₁ was 73% of predicted value and expiratory wheezing sound was observed on whole lung field. FEV₂was decreased to 48.9% of baseline on 5 hours 30 minutes, respectively, after exposure of hydrogen chloride at workplace. On follow up examination, the patient complained of an intermittent dyspnea and cough.

만성두통 환자의 성격유형 A 행태, 스트레스, 우울 및 두통영향정도의 관계 연구

차남현,임사비나,정인태,김수영,안경애,김건식,이재동,이상훈,최도영,이윤호,이두익 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-

To examine an estimate factor and grasp the relation of difference for Ype A Behavior Pattern(TABP), Perceived Stress Questionnaire. Depression and HIT-6 in the Chronic headache client. Method: Data collected by self-reported questionnaires from 38 client in S city who were selected by criteria of IHS, from the 19^(th) of October to 10^(th) of December, 2004. Result: 1) Differences between biographical data by TABP was significant by SaSang constitutions, by Stress was significantly influenced by age, and by Depression were significantly influenced health status and SaSang constitutions. 2) Correlations Coefficients among Study Variables were Stress and Depression(r=.494, p=.002) and Depression and HIT-6(r=.432, p=.010). 3) In regression analysis, HIT-6 were significantly influenced by Depression and Type A Behavior Pattern(TABP). These variables explained 38% and 34% respectively. Conclusion: The result suggest that chronic headache management with psychological aspect, as well as physical aspect should be a focus to enhance the quality of life.

Most Probable Number 방법을 이용하여 측정한 중랑천 河床土壤의 혐기성 세균의 수와 수질과의 상관 관계

박두현,김병홍,임시근,최영효 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.3

중랑천의 하상 토양의 혐기성 호흡세균의 분포를 MPN법으로 조사하였다. 철이온환원세균을 조사하기 위해 배양액 중의 Fe(Ⅱ)을 확인하는 변형된 oxalate-ferrozine 법을 개발하였으며, 황산염환원세균(SRB)과 메탄생산세균을 각각 FeS 침전과 head space의 CH_4를 검출하는 방법을 채택하여 중랑천 지천과 본천의 혐기성 미생물 분포를 조사한 결과 오염 정도가 상대적으로 높은 본천에서 철이온환원세균이 10^7~10^8 cells/g 정도로 조사되었으며 지천의 경우 10^7 cells/g이었다. 우이천 상류를 제외한 모든 조사 지점에서 SRB와 methanogen 이 10^3 cells/g이었다. 이 결과는 분류 하수관의 설치로 유기물의 유입이 낮아진 조건에서도 하상의 침전층에서 유래하는 유기물을 이용하여 철이온환원세균이 생장할 수 있으며, SRB와 methanogen의 생장은 철이온환원세균에 의해 방해를 받는다는 것을 나타낸다. Sediments collected from the Jungnang-cheon and its tributaries were used to enumerate anaerobic bacteria by most probable number (MPN) methods. A simple method was developed to detect ferrous ion in the culture fluid in order to count the number of iron ion reducers, and sulfate-reducing bacteria (SRB) and methanogens were detected by the presence of FeS precipitate in the culture or methane in the head space, respectively. The numbers of iron reducer was in the range of 10^7~10^8/g in the sediment of the stream containing higher organic content than the tributaries. The sediments of tributaries were analyzed to contain iron reducers less than 10^7 cells/g. With one exception the numbers of SRB and methanogens were less than 10^3 cells/g in the sediment. From these results it is concluded that organics in the sediment support the growth of iron reducers, which out-compete SRB and methanogens.

드라이 용제(dry solvent)를 취급하는 근로자들의 자각증상

김수근,임현술,김두희 東國大學校醫學硏究所 1997 東國醫學 Vol.4 No.-

드라이 용제를 취급하는 근로자들의 건강장애를 알아보기 위하여 드라이 용제 폭로 근로자 51명과 동일 사업장의 사무직 근로자 97명을 대상으로 자각증상 호소율을 조사하고, 건강진단을 통해 간기능검사(혈청 GOT, 혈청 GPT)와 혈액검사(혈색소량, 혈구용적치, 백혈구수)를 실시하여 다음과 같은 결과를 얻었다. 1. 중추신경계 관련증상과 일부 자극증상이 폭로군에서 비교군보다 통계적으로 유의하게 높았다. 최근 1주일간 작업 중에는 '머리가 무겁게 느껴진다', '눈이 침침하다', '두통이 있다', '어지럽다' 순이었고, 지난 6개월 동안에 경험한 증상은 '몸이 나른하다', '눈이 쉽게 피로해진다', '머리가 무겁게 느껴진다', '현기증이 난 적이 있다(어지럽다)', '눈이 침침하다', '머리가 띵하다' 순이었다. 2. 혈액검사 결과는 1명의 철결핍성 빈혈 외에는 모두 정상이었다. 간기능 검사상 이상소견율은 폭로군에서 높앗으나 통계적으로 유의하지 않았고, 연령, 근속기간, 비만도, 흡연 유무, 음주습관 등을 고려한 로지스틱회귀분석에서도 폭로군의 교차비(odds ratio)가 유의하게 높지 않았다. 이상의 결과에서 드라이 용제에 폭로된 근로자들에서 만성 중추신경계장애로서 기질적 정서증후군에 해당하는 자가증상의 호소율은 증가하나, 혈액학적 소견의 변화와 간기능장애는 관찰되지 않았다. 앞으로 드라이용제에 폭로되는 근로자들의 건강장애를 파악하기 위해서는 자각증상의 조사가 중요하며, 개인별 폭로량을 계량화하여 건강장애를 심층분석하는 것이 필요하다. This study have following results by means of complaint rate of subjective symptoms, liver function test(SGOT, SGPT) and hematological test(Hb, Hct, WBC) in order to investigate the impact of exposure to dry solvent on the health, the subject of study is 51 dry solvent exposed worker and 97 office worker in the same plant. 1. In the complaint rate of subjective symptom, exposed workers are higherthan office workers on the CNS depression and irritation. During working time, they had following symtom in order ① heavy feeling in the head ② dimmed vision ③ headache ④ dizziness. During ordinary time, they had following symtom in order ① general weakness ② strained eye ③ heavy feeling in the head ④ dizziness ⑤ dimmed vision ⑥ light headedness 2. In hematological findings, they had almost within normal limit except one case of iron deficiency anemia. In abnormal rate of liver dysfunction, exposed workers are higher than office workers. But it is'nt significant staistically. Also, in logistic regression analysis which is controlled about age, working career, BMI, smoking habit and weekly alcohol intake, odds ratio of exposed workers aren't higher significant staistically than office workers. As these result, it is recognized that organic affective sydrome in solvent induced-CNS disorders result in when workers exposed dry solvent. But there is no evidence of abnormal hematologic findings and liver dysfunction due to dry solvent exposure. Therefore, the survey onsubjective symptom questionnaire is necessary to recognize health impact of dry solvent, and it is necessary to investigate on health impact according to exposure level of dry solvent.

만성두통환자에게 적용한 비약물적 치료가 통증행동에 미치는 영향

최도영,임사비나,차남현,김건식,이재동,김수영,이윤호,이두익 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-

Objectives : This study is a random-controlled post-design for confirmation of headache degree, quality of life, and satisfaction to therapeutical effect on chronic headache adults after management of non-medicinal treatment (acupuncture therapy and stellate ganglion block therapy). Methods : 51 clinical experiment participants were gathered and through a questionnaire patients who experienced headache for more than 4 hours a day and more than 15 days per month were qualified as chronic headache patients. The qualified patients were classified in to two groups, acupuncture group (AT group, n=28) and stellate ganglion block group (SGB group, n=23). Treatment was applied 2 times a weeks for 4 weeks. The effects of both groups were analyzed using VAS scores, BPI (Brief Pain Inventory) and the satisfaction degree to the therapy. Results : 1. The recognized score of the headache of AT group and SGB group was reduced indicating the degree of the headache was released. 2. The recognition at damage to the quality of life was reduced post therapy of AT group and SGB group, in which general activity, mood, enjoyment of life, personal relationship, and sleeping showed significant improvement of life quality. 3. The satisfaction degree to the therapy showed lower score than expectation to it in AT group and SGB group, however, it was not significant. Conclusions : The results showed that the four-week non-medicinal treatment (AT and SGB) in chronic headache patients was effective for reducing headache and releasing its damage in daily life, however, no difference in superiority was found. Therefore, non-medicinal treatment (AT therapy and SGB) could be utilized in chronic headache patients.

분뇨 처리용 선박 저장탱크에서 발생한 황화수소로 인한 사고 증례

김덕수,임현술,김두희 동국대학교 의학연구소 2000 東國醫學 Vol.7 No.-

본 증례는 폐기물 저장탱크에 장기간 보관된 분뇨를 청소하던 4명의 선원이 급성 중독으로 2명은 사망하고 2명은 생존하였다. 다음날 실시한 작업환경 측정결과 황화수소 농도가 150 ppm 이상 측정되어 황화수소 중독에 의한 사고로 판명되었다. 사망자의 사망원인은 황화수소 중독으로 의식을 잃고 쓰러지면서 저장탱크에 고인 물속에 잠겨 기도 확보가 되지 않아 생긴 익사로 추정된다. 생존자는 100% 산소와 지지치료 만으로 회복되었다. Hydrogen sulfide (H2S), one of the most poisonous gases, is usually encountered in various occupations as a byproduct. Four crew were collapsed in a waste storage tank while they were cleaning it. Immediately they were rescued, but two crew died and two crew survived. Gas analysis in the waste storage tank showed high concentrations of hydrogen sulfide (above 150 ppm) after one day. The causes of death were asphyxiation because their face fell into mixed water with collapsed state, not due to H2S intoxication. The victims completely recovered with supportive care, including 100% normobaric oxygen.

메타분석을 통한 가정특성 관련변인이 학업성취에 미치는 영향

박성열,구병두,임현걸 건국대학교 교육연구소 2000 論文集 Vol.24 No.-

This paper was designed to synthesize quantitatively the master's theses and doctoral dissertations focusing on effects of family background traits related variables on academic achievements in Korean. This task was accomplished by utilizing the meta-analysis technique of research integration as defined by Glass(1982). Basically, meta-analysis is a statistical analysis of the summary findings of many empirical studies. Its purpose is to draw reliable and general conclusions form a large and complex body of literature on a common topic. Thus, the primary purpose of this paper was to conduct a quantitative synthesis of 96 master's theses and doctoral dissertations focusing on the effects of academic achievements using family background traits as independent variable and academic achievement as dependent variable. Nine independent variable were chosen by means of in depth review of those previously mentioned 96 studies. The independent variables employed in this study include psychologcal environment, physical environment, family structure, public and private educational expenditures, education process, breeding and education method of parents, socio-economic status, parents' characteristics, and role expectation. These variable are cartegorized by two different groups according to the traits of factors in terms of family environment variable group and parent's characteristics variable group. This study set the following questions to be answered: (1) How large are the grand average effect size of variables of family background traits proposed in this study on student's academic achievement?; (2) How different the effect size of the two family background traits related variable groups, naimly family environment and parents characteristics variable group on student's academic achievement?; and, (3) How different the effect size of the nine family background traits related independent variables on student's academic achievement? In order to answer the research questions, the researcher followed the standard procedures as such locating published studies to collect primary data, analysing and cording the characteristics and outcomes of the studies, and carring out statistical procedures of mata-analysis to describe the overall and sub-variable effects. Primary source of data was decided to limit within the master's these and doctoral dissertations in order to achieve the reliability and validity of the study results. By using the CD-ROM titled 'masterㆍdoctoral dissertation index' published by National Central Library in 1995 and 1997, a total of 112 studies were located and collected. Out of 112 studies, 96 studies were selected by excluding those studies that are not possible to employ mata-analysis such as studies used qualitative research method. While classifying family background traits related variables some of studies were also excluded in this study due to failing a consensus among the five specialists participated in this process. The effect size were calculated by using two different approaches depending upon the applicability of effect size formula. One was by using mean and standard deviation of experimental group and their countpart. The other was using transformational formula suggested by Wolf(1986) for the studies that have not presented descriptive statistics. The result of this study may be summarized as follows: (1) The grand average degree of effects of the academic achievement factors proposed in this study turned out to be .33. This means that if the average size of effects of the academic achievement factors of the controlled group is 50 percentile in normal distribution, that of the experimental group becomes 62.93 percentile. As it were, the latter surpass the former by 12.94 percentile. (2) Between the two groups of academic achievement factors proposed in this study, in terms of family environment variable group and parent's characteristics variable group, the former factors showed the higher average effect size, its average effect size being .35. Considering the grand average degree of effects size of .33,it can be suggested that those family environment related variables effects more on the student's academic achievement compare to the variables of parents' characteristics. Among the nine family background traits related independent variables on academic achievements proposed in this study, the variable of educational process showed the highest average effect size of 1.40, as followed by socio-economic status(effect size of 0.55),role expectation(effect size of 0.48), and physical environment(effect size of 0.40). The effect size of these variables turned out to be bigger that the grand average effect size of .33. This result means that these variable effects on student's academic achievement a lot more compare to other variable proposes in this study. In fact, the effect size of those variable such as phychologcal environment(effect size of 0.27), breeding and educational method of parents(effect size of 0.27), family structure(effect size of 0.26), public and private educational expenditures(effect size of 0.26), and parents' characteristics(effect size of 0.14) turned out to be much smaller than that of previously mentioned variables. It means that the variable of psychologcal environment, breeding and education method of parents, family structure, public and private educational expenditures, and parents' characteristics effect less on student's academic achievement compare to the variables of educational process, socio-economic status, role expectation, and physical environment.

Glucose/Hydrogen Peroxide Redox System을 사용한 견직물의 저온염색

이내연,백두현,임종열,임영훈 충남대학교 공업교육연구소 1993 論文集 Vol.16 No.1

The low-temperature dyeing system for silk fabrics based on a redox system has been investigated. Some factors affecting dyeing of silk fabrics with levelling acid dyes in the absence and presence of certain redox system were investigated under different conditions. The variables studied were the type and concentration of redox system with different dyeing conditions, i.e. temperature and time, dye concentration, material-to-liquor ratio (LR) and colour fastness. The colour strength (K/S value) is remarkably higher in the presence than in the absence of redox system. In the presence of redox system, free radicals are supposed to the formed both the fiber and the dye, and the interaction between free radicals bring about covalent fixation beside the usual electrostatic bonds, hydrogen bonds and Van der Waals forces.

만성두통환자에 대한 동서협진의 임상적 연구

정인태,이상훈,김수영,차남현,김건식,이두익,이재동,임사비나,이윤호,최도영 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-

Objective : Because the cause and etiology of chronic headache are not yet fully explained, the treatment of this symptom is not simple. In order to study the effectiveness East-West pain treatment on chronic headache, we evaluated its effect of pain alleviation and quality of life improvement on chronic headache patients who were treated with nerve block and acupuncture, and only acupuncture for eight weeks. Methods : 92 clinical experiment participants were gathered and through a questionnaire patients who experienced headache for more than 4 hours a day and more than 15 days per month were qualified as chronic headache patients. The qualified patients were classified into two groups, nerve block and acupuncture group(EW group, n=49) and acupuncture group(E group, n=43). Treatment was applied 2 times a weeks for 8 weeks. The acupoints, GV20, HN23, ST8, HN46, TE17, GB20, LI20, LI11, LI14, ST36, and LR3 were stimulated for 20 minutes. The effects of two groups were analyzed using VAS scores and BPI(Brief Pain Inventory). Results : The VAS and BPI after four weeks of treatment showed significant improvement among two but differences between the three groups was statistically insignificant. The VAS and BPI of the EW group after eight weeks of treatment showed statistically significant improvement compared to the other two groups. Conclusion : The pain treatment for chronic headache might be contributed to improvement of quality of life as well as alleviation of pain of chronic headache patients. It was suggested that the West pain treatment in combination with East pain treatment might be an useful modality to improve the pain and quality of life in chronic headache patients.