Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

하악골 절제술후 재이식된 돌결자가골 재이식 : 치험 3례

김 식,김수관,김운규,김수민,류종희,이준길,조경안,김수흥,정태영,여환호 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.2

We described three cases of immediate reimplantation of a frozen-thawed autogenous mandible composed of a mixture of iliac bone, marrow, and particulate hydroxyapatite in tumors of the mandible. Acceptable outcomes were obtained in three patients who underwent immediate autogenous mandibular graft reconstruction. The conditions leading to successful outcome of the procedure are also discussed. Reimplantation of frozen autogenous lesioned mandible was performed in three patients with mandibular tumors. Two reimplanted grafts survived without complications following surgery. One case had postoperative infection that resolved with appropriate antibiotic treatment. There were no recurrences of the primary lesions. Satisfactory facial contour after surgery was achieved. These results are most promising, and we believe that, with further refinement, this technique will offer a new and acceptable modality for facial reconstruction in patients with cancer.

忠南黑石溪谷 住民의 肺, 肝디스토마 感染率 및 그 中間宿主 調査

金鍾煥,鄭奉晳,金玉千 醫藥係社 1955 綜合 醫學 Vol.12 No.12

유도 허벅다리걸기 기술발휘 시 받기의 자세와 저항수준에 따른 중심변인 분석 사례연구(Ⅱ)

김의환,김성섭,정재욱 한국운동역학회 2005 한국운동역학회지 Vol.15 No.1

E. H. KIM, S. S. KIM, C. W. CHUNG. A Case Study on Center of Gravity Analysis when Performing ilchimata by Posture and Voluntary Resistance Levels of Uke in Judo[ II]. Korean Journal of Sport Biomechanics, Vol. 15, No. 1, pp. 237.-257, 2005. It was to study as a following-research of "A Case Study on Center of Gravity(COG) Analysis when Performing Uchimata(inner thigh reaping throw) by Posture and Voluntary Resistance Levels(VRL) of Like in Judo[1]". The purpose of this study was to analyze the COG variables when performing uchimata(inner thigh reaping throw) by two postures and voluntary resistance Jevels(VRL) of uke(reciver) in Judo. The subjects, who were one male judoka(YH) for 1992 Barcelona Olympic Games Olympian(silver medalist), and one male trainee; Y.I.University representative member (SDK), and were filmed on two S-VHS 16mm video cameras(60fields/sec.) through 3-dimensional motion analysis methods, that postures of uke were shizenhontai (straight natural posture) and jigohontai(straight defensive posture), VRL of uke were 0% and 100%, respectively. The kinematical variable was COG variable, distance of COG, and distance of resultant COG between uke and tori(the thrower), velocity and acceleration of COG. The data of this study collection were digitized by SIMI Motion Program computed the mean values and the standard deviation calculated for each variables. When performing uchinwta according to each posture and VRL of uke and classifying. From the data analysis and discussion, the conclusions were as follows : 1. Displacement of COG Subject YH, COG was the highest in kuzushi(balance -breaking), vertical COG was low when following in tsukuri(positioning; set-up), kake(application; execution), and COG was pattern of same character each postures and resistance, respectively. Subject SDK, COG was low from kumikata(engagement positioning) to kake, and COG was that each postures and resistance were same patterns, respectively. Subject YH, SDK, each individual, postures and resistance, vertical COG was the lowest in kake phase, when performing. 2. Distance of COG between uke and tori The distance of COG between uke and tori when performing, subject YH was 0.64-0.70cm in kumikata, 0.19-0.28cm in kake, and SDK was 0.68-0.72cm in kumikata, 0.30-0.42 cm in kake. SDK was wider than YH. 3. Distance of resultant COG between uke and tori The distance of resultant COG between uke and tori when performing, subject YH was 0.27-0.73cm from kumikata to kake. and SDK was 0.14-0.34cm in kumikata, 0.28- 0.65cm in kake. Jigohontai(YH:0.43-0.73cm,SDK:0.59-0.65cm) was more moved than shizenhontai(YH:0.27-0.53cm, SDK: 0.28- 0.34cm). 4. Velocity of COG The velocity of COG when performing uchimata, subject YH was fast anterior-posterior direction in kuzushi, ant-post. and vertical direction fast in tsukuri and kake. SDK was lateral, ant.-post. and vertical direction in kuzushi, ant.-post. and vertical direction in tsukuri and ant.-post. direction in kake, respectively. 5. Acceleration of COG The acceleration of COG when performing uchimata, The trend of subject YH was showed fast vertical direction in kuzushi and tsukuri, ant-post. and vertical direction fast in kake. The trends of SDK showed lateral direction in kuzushi, lateral and ant.-post. direction in tsukuri and ant.-post direction in kake, respectively.

네모나프라이드의 유효성 및 추체외로 부작용 : Haloperidol 및 Risperidone과의 자연관찰적 개방형 비교연구 A Naturalistic Open Comparative Study with Haloperidol and Risperidone

서영수,김용관,신동환,공보금,이정구,박정환,윤성환,정치영,이상경,김영훈 대한생물치료정신의학회 2001 생물치료정신의학 Vol.7 No.2

Objectives : This open prospective study was performed in order to evaluate the efficacy and extrapyramidal adverse effects of nemonapride in the schizophrenic patients, and was compared wit one of typical antipshchotics. haloperidol and one of atypical antipsychotics, risperidone. Methods : Thirty male and female schizophrenic patients(DSM-Ⅳ) were treated for 12 weeks with haloperidol(n=10), risperidone(n=10) and nemonapride(n=10). The overall efficacy was assessed at baseline, 1st week, 2nd week, 4th week, 8th week, and 12th week by the Positive and Negative Syndrome Scale for Schizophrenia(PANSS). Also the overall safety was assessed in the same time period by the Extrapyramidal Symptom Rating Scale(ESRS). Results : There were no significant differences in PANSS scores(total, positive, negative, and general psycho­pathology subscale) among haloperidol, risperidone, and nemonapride groups, Treatment responders, defined as at least 20% reduction of baseline total PANSS score, were achieved by 8 patients(80%) in haloperidol group, 10 patients(100%) in risperidone group, and 8 patients(80%) in nemonapride group. And there were no significant differences in ESRS total scores among haloperidol, risperidone, and nemonapride group. Inter-group comparison among haloperidol, risperidone and nemonapride group, as assessed by the ESRS, revealed no significant differences in the shifts to the maximum score, 9.4(±9.4), 6.2(±8.4), and 11.3(±8.1) respectively, and also revealed no significant differences in the mean time reaching the maximum score, 4.5(±4.5) week, 7.8(±4.8) week, and 4.6(±4.4) week, respectivily. Conclusion : There wee no significant differences in the efficacy and extrapyramidal adverse effects among haloperidal, rispecridone and nemonapride groups. These results suggest that nemonapride was as efficacious and safe in he treatment of schizophrenia, as well known and widely used antipsychotics, haloperidol and risperidone.

광양만으로 유입하는 하천하구에서의 이화학적 특성 : 우수기 직후 유입하천에 따른 영향 effect of inputting rivers after rainy season in summer

김은영,정흥호,정호승,조환익,황주찬,최상원 한국환경과학회 2004 한국환경과학회지 Vol.13 No.9

Physicochemical characteristics of Kwangyang Bay, have been investigated on the effects of inputting rivers just after rainy season in summer 2003. The results ranged from 4.96~8.01 mg/L for DO, 1.16~3.09 mg/L for COD, 7.21~67.71 μM for DIN and 0.12~121.84 μM for phosphate. The COD of Yeosu Area was 1.19~3.09 mg/L, Kwangyang Area was 1.23 ~ 2.76 mg/L. The COD of Wallae cheon in Yeosu Area was 3.09 mg/L and Donghoan in Kwangyang Area was 2.76 mg/L. The reason of high COD might be industrial sewage of organic chemical plants and iron foundry. DIN of Yeosu Area and Kwangyang Area were 7.21--63.43 μM and 8.43 67.71 μM respectively. The average phosphate of Yeosu Area was 23.65 μM. The source of phosphate might be industrial sewages of fertilizer plant and phosphate gypsum stocked with high amounts. The metal concentrations were measured in the range of 1.01~175.10 ㎍/L for Cu, 0.13~1.78 ㎍/L for Cd, 1.13~5.21 ㎍/L for Pb, 1.58~13.57 ㎍/L for Cr and 1.07~20.68 ㎍/L for As. The average Cu concentration was 78.99 ㎍/L in Kwangyang Area. The source of Cu might be industrial sewage of iron foundry. The average Cr concentration of Yeosu Area was 9.07 ㎍/L. That is 2 times higher than Kwangyang Bay 5.74 gg/L, because of sewage of chemical plants.

고속 플라즈마 진단용 능동회로의 설계 및 응용

김두환,이우근,박정후 釜山大學校生産技術硏究所 1995 生産技術硏究所論文集 Vol.49 No.-

본 논문에서는 자화 플라즈마의 파라메타를 측정할 수 있는 고속 푸르브 구동시스템을 구현하였다. 이 시스템은 1[msec] 이내에 -40 - +40[V]에 이르는 톱니파 전압을 푸르브에 인가시킬 수 있을 뿐만 아니라, 2회 연속 미분을 행하여 전자에너지 분포함수를 구할 수 있었다. 이와 같이 고속 푸르브 구동시스템 및 2회 연속 미분회로를 이용하여 고속으로 거의 전형적인 푸르브 특성곡선을 얻을 수 있었고 2회 연속 미분회로로써 얻어진 전자에너지 분포하수에서 플라즈마의 중심부 보다 플라즈마의 가장자리에 고에너지를 가진 전자들이 많이 분포함을 알 수 있었다. In this paper ,we have realized the high speed probe driving circuit system which can measure the plasma parameters of magnetoplasma. By this system, the probe voltage can be seeped in a 1[msec] order. In addition, we can obtain the electron energy distribution function in magnetoplasma by using two differentiators. In this study, we obtain a complete probe characteristics, and find that the electron energy measured at the edge of plasma is larger than of electron measured at the center of plasma

치근단X선사진을 이용한 만곡치의 연구

김정우,황의환,이상래 大韓口腔顎顔面 放射線學會 1990 Imaging Science in Dentistry Vol.20 No.2

The purpose of this study was to evaluate the incidence and several radiographic features of dilacerated teeth in 2132 full month radiograms in Korean. The results were as follows : 1. The occurrence was revealed to 2.7% in total examined teeth, and these anomalies were occurred in maxilla(52.4%) more than in mandible(47.6%). 2. There was a predilection for occurrence of dilaceration in female, which included 12.8% of the female compared to 11.3% in male. 3. The frequency of dilaceration in male, which included 40.5% in maxilla and 59.5% in mandible. The frequency of dilaceration in female, which included 52.4% in maxilla and 47.6% in mandible. 4. The order of frequency of dilaceration was second premolar, first premolar, lateral incisor, canine in maxilla, and first premolar, second premolar, canine, lateral incisor, central incisor in mandible. 5. In classifying of dilacerated teeth into 3 types by following appearances such as root curvature. Distal dilaceration was by far most common containing 65.8% of the cases. The least frequent was mesial dilaceration, which included 6.5% of the cases.

경부 종괴의 초음파 소견

김진환,김충현,이병철 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

The 48 sonograms of 46 patients with neck masses were retrospectively reviewed to assess the superiority of ultrasonography. Neck masses were divided into salivary glands, thyroid gland, cervical lymph node origin masses, and others. The salivary gland masses(16 cases), included pleomorpic adenoma(5 cases), sialadenitis (9 cases), and abscess (2 cases). The thyroid gland masses(7 cases), included thyroiditis(2 cases), goiter(4 cases) and malignant tumor(1 case). The cervical lymphadenopathy(10 cases) included Inflammatory(7 cases) and neoplastic(3 cases). The other masses included branchial cleft cyst(2 cases), thyroglossal duct cyst(2 cases), cystic hygroma(2 cases), abscess(2 cases) etc. Real-time ultrasonography with water bag adapted linear array 5.00 MHz transducer enabled us to ascertain mass character in detail. Sonography is very useful for differentiating a soild lesion from a cystic one, for differentiating an intraglandular lesion from a superficially located lesion extrinsic to the gland. On the other hand, CT is the method of choice for seperating lesions of the deep portion of the neck from adjacent structures. The sonography does not visualize the entire deep portion at the neck but it is not a major drawback of the method because majority of lesion originate from the superficial portion

프로스타글란딘 E₁ 마이크로에멀젼이 함유된 액상좌제의 제조 및 평가

김정환,이사원,박경미,최한곤,김종국 영남대학교 약품개발연구소 2000 영남대학교 약품개발연구소 연구업적집 Vol.10 No.-

The purpose of this study is to develop a transurethral liquid suppository containing prostaglandin E_(1)(PGE_(1))-loaded microemulsion, which undergoes a phase transition to gels at body temperature. The effects of oils, ethanol as solvent and HCI as pH-controlling agent on the physicochemical properties of liquid suppositories composed of poloxamer P 407. P 188 and carbopol was investigated. The stability of PGE_(1) and release of PGE_(1) from liquid suppository were evaluated. Oils such as Neobee and soybean oil significantly decreased the gelation temperature but increased the gel strength of liquid suppository due to their strongly binding with the components of liquid suppository base. However, ethanol slightly did the opposite. The pH of liquid suppositories hardly affected the gelation temperature and gel strength due to addition of very small HCI(0.005-0.01%). A liquid suppository [PGE_(1)/P 407/P 188/carbopol/Neobee/ethanol/HCI(0.4/14/14/0.4/7/2/0.005%)], which had the gelation temperature (34.2±0.6℃) and gel strength (31.35±4.37 sec) suitable for liquid sup-pository system, was easily administered and not leak out from the body. About 60% of PGE_(1) was released out within 2h from this formulation. It was shown that the release of PGE_(1) was proportional to the square root of time, indicating that PGE_(1) might be released from the suppository by Fickian diffusion. It was stable at 4℃ for at least 2months. The results suggest that transurethral liquid suppository containing prostaglandin E_(1)-loaded microemulsion is thought to be a con-venient, safe and effective dosage form for PGE_(1). However, it should be further developed as a more convenient and stable dosage form for PGE_(1).