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      • 天然洗劑의 生物分解에 관한 硏究

        이춘식,최종수,박승조 동아대학교 환경문제연구소 1993 硏究報告 Vol.16 No.1

        We were investigated with biodegradation of natural detergent by using jar-test, column test and activated sluge process. We were studied the BOD loading rate, the removal rate of organic material, the relationship between F/M, ratio, BOD and SS, SRT and MI, VSS/MLSS ratio. The results are summarized below. 1. In case of column height 160cm, suspended solid removal rate was 60.93%, COD, BOD and SS removal rate were 40%, 33% and 87% repectively in jar-test. In this case dosage of coagulant was 40.9g/㎥ alum, 4.5g lime/㎥ and 0.4g/㎥ polymer. 2. Wastewater containing natural detergent concentrationabout 600-1300mg/l was treated with activated sludge process and then removal rate of BOD is 80% in winter atmospheric temperature. In this case BOD concentration was about 10mg/l. 3. Wastewater containing natural detergent was treated with activated sludge process and then BOD volumetric loading rate is 0.3-0.8kg BOD/㎥·d. In this case BOD removal rate was about 90 %, MLVSS/MLSS ratio was 0.4-0.5 and SVI is 60-200.

      • 그루지아 민가의 전통 발효유에서 분리한 유산균의 이화학적 특성

        신승이,최기춘,김종현,박정수,이영환 全南大學校 農業科學技術硏究所 1999 農業科學技術硏究 Vol.34 No.-

        그루지아의 전통 발효유로부터 유산 생성과 항균성이 우수한 3주의 균주를 최종 분리, 선발하고 각각 Lactobacillus bulgaricus (L bulga G82), Lactobacillus acidophilus (L. acid G86), 그리고, Streptococcus thermophilus (S. thermo G90)로 동정하였다. 이들 유산균을 starter로 요구르트를 제조하여 이화학적 특성을 조사한 결과, pH는 4.0∼4.5의 범위를 보였으며, pH 4.0으로 L. acid G86 균주가 가장 낮게 나타났다. 또한 0.1N HCl에 대한 완충능은 4.50∼5.12ml, 0.1N NaOHdp 대한 완충능은 3.74∼4.98ml의 범위를 보였고, 3 균주 중 L acid G86 균주가 산-염기에 대하여 가장 높은 완충능을 나타내었다. 산도는 0.95∼1.16%, 점도는 1,984∼2,232 cps 그리고 생균수는 1.4x109∼3.0x109으로 조사 되었다. in vitro에서의 콜레스테롤 저하 정도는 L. acid G86 균주가 약 35%로 가장 높은 저하 정도를 보였고, L. bulga G82와 S. thermo G90 균주는 각각 약 32%, 24%이었다. Three strains of inhibitory lactic acid bacteria against pathogenic microorganism were isolated from traditional fermented milk of Georgia, and than, identified to be Lactobacillus bulgaricus (L bulga G82), Lactobacillus acidophilus (L acid G86) and Streptococcus thermophilus (S. thermo G90), respectively. These strains were used to starter for yogurts. So, we made yogurts, and evaluated their phyco-chemical properties. The range of pH value was 4.0∼4.5, and L. acid G86 strain was the lower pH value for 4,0. The buffer capacity against 0.1N HCI and 0.1N NaOH were 4,50~5.12ml and 3.74~4.98ml. The acidity, viscosity and viable cell counts of yogurt were 0.95∼1/16%, 1.984∼2,232 cps and 1.4x109∼3.0x109, respectively. Among the three strains, L. acid G86 strain was have the best ability of lowering cholesterol level in vitro, the value were about 35%. The L. bulga G82 and S. thermo G90 were around 32% and 24%, respectively.

      • 市乳의 貯藏期間에 따른 耐冷性 微生物 및 化學的 特性의 變化에 關한 硏究

        이수원,강일수,양희진,백승천 성균관대학교 생명과학자원연구소 1997 生命資源科學硏究 Vol.4 No.1

        The present study was conducted to predict shelf life of pasteurized milk with analysis of volatile compounds and sensory evaluation of milk to growth of psychrotrophic bacteria. Pseudomonas fluorescens KFRI 00194 inoculated concentration of 10^4 cfu/㎖ to observe of changes on sensory evaluation and volatile compounds of pasteurized milk. The results obtained in these experiments were summarized as follows; The psychrotrophic bacteria number at 4℃ were increased 6.39×10 exp(4)cfu/㎖ until first day and 2.20×10 exp(6)cfu/㎖, 1.17×10 exp(7)cfu/㎖ after 5 and 10days, respectively. The volatile compounds were shown the decreasing tendancies in acetaldehyde, ethanol depending on storage days, especially ethanol contents were significantly decreased between 5 and 10days storage(P<0.05). Diacetyl, 2-heptanone, 2-butanone and 3-methyl-1-butanol were shown the increasing tendencies of contents. Diacetyl, 2-heptanone and 2-butanone were significantly increased between 5 and 10days, 1 and 5days, 5 and 10days and 1 and 5days, respectively(P<0.05). However, 3-methyl-1-butanol was shown a increasing tendancy depending on storage days but the significantly different was not recognized. The changes of sensory evaluation were rapidly decrease from 6.8point first day to 4.2point after 10days and the decrease of organoleptic acceptability was significantly different recognized(P<0.05), excepted of 1 and 2days. The organoleptic acceptability according to increasing of psychrotrophic bacteria numbers were decreased. The organoleptic acceptability depending on changes of volatile compounds were shown to a conversed correlation coefficient with the exception of acetaldehyde and acetone. Especially, 2-heptanone, 2butanone and 3-methyl-1-butanol were gradually decreased of acceptability by the contents.

      • 휘발성 미량유기오염물질의 광분해에 관한 연구

        이춘식,최장승,안종수,박현건,성낙창,박승조 동아대학교 환경문제연구소 1998 硏究報告 Vol.21 No.1

        This study was carried out photolysis of Trace Volitile Organic Pollutants(BTEX) in the water. The experiment results were summarized as follows : 1. Benzene of removal efficiency were revealed 90% on illumination time of 20minute for bezene concentration range 0.01ppm-0.06ppm by 450W medium pressure mecury lamp of photon energy photolysis reaction, and were revealed 93% phomoxidaton on illumination time 30minute in 0.lppm of reactant benzene. Toluene of removal efficiency were revealed each 97.7%, 98.3%. 97.7%, 87.396 on illumination time of 20minute for toluene each of concentration 0.0lppm, 0.03ppm, 0.06ppm. 0.1ppm by 450W low pressure mecury lamp of photon energy photolysis reaction, and reactant toluene was disappeared on illumination time 30minute. 2. Ethylbenzene of removal efficiency get the better photolysis on the treatment contrast ethylbenzene with benzene and photolysis removal efficiency was above 90% in ethylbenzene concentration range 0.0lppm-0.lppm on illumination time l0minute. 3. Photolysis removal efficiency of m, p-Xylene were researched better result 93% on the about l0minute of illumination time m, p-Xylene concentration range 0.01ppm-0.06ppm, removal efficiency was 97% in higher concentration of 0.2ppm and illumination time 20minute. 4. Photolysis removal efficiency of o-Xylene were researched better result 95% on the about l0minute of illumination time m, p-Xylene concentration range 0.0lppm-0.lppm, and reactant o-Xylene was disappeared on illumination time about 20minute by 450W m d u m pressure mecury lamp.

      • KCI등재
      • Ciprofloxacin의 동물유래 병원세균에 대한 시험관내 항균효과시험

        장경수,장치훈,김일택,박승춘,윤효인,전무형 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

        In this study the antimicrobial spectrum and sensitivity of ciprofloxacin(CFX) against the major pathogenic bacteria isolated from the diseased poultry, pig, cattle in Korea were evaluated in comparison with morfloxacin(NFX), enrofloxacin(EFX), nalidixic acid(NA), gentamicin(GM), tetracycline(TC), erythromycin(EM), streptomycin(SM) and penicillin(PC). Increasing by paper disk diffusion test for total of 439 isolates from poultry, pig and cattle, CFX showed remarkably higher sensitivity(>83%) as compared with other drugs. When three quinolones such as CFX, NFX and NA were compared for the inhibition activity against 4 major pathogens, CFX induced significantly larger diameter of inhibition zone through all of the tested concentration than NFX and NA. As MICs of all drugs for 11 bacterial species isolated from poultry were measured, the MIC range of CFX was 0.125 - 40 ㎍/㎖ in Gram positives, ≤ 0.005 - 2.5 ㎍/㎖ in Gram negatives and 0.08 - 1.25 ㎍/㎖ with 12 isolates of Mycoplasma. MIC_50 and MIC_90 of CFX in these cases revealed consistently lower as compared with those of NFX, GM and PC. When the MICs of the drugs were tested with 11 bacterial species from the diseased pigs, MIC range of CFX was 0.05 - 3.5 ㎍/㎖ in Gram positives, ≤ 0.005 - 0.8 ㎍/㎖ with Gram negatives and 0.5 - 9.0 ㎍/㎖ with 11 isolates of Mycoplasma. MIC_50 and MIC_90 of CFX and NFX were remarkably lower than those of GM and PC. As MICs of CFX, NFX, GM and PC for 10 bacterial species isolated from cattle were tested, the MIC range of CFX was found 0.01 - 4.0 ㎍/㎖ in Gram positives, and ≤ 0.005 - 0.2 ㎍/㎖ in Gram negative bacteria. The MIC_50 and MIC_90 of CFX in cases of cattle appeared remarkably lower than those of the other drugs. CFX, as compared with the other drugs, showed invariably lower MIC_50 and MIC_90 in both of GRam positives and negatives. As MICs of CFX, NFX and PC for 12 reference bacteria were examined, MIC of CFX was 0.03 - 0.25 ㎍/㎖ that was much lower drug concentration than those of NFX(0.25 - 1.0 ㎍/㎖) and PC(0.5 - 16.0㎍/㎖).

      • KCI등재

        성형조건에 따른 무연탄계 활성탄의 세공특성

        이송우,나영수,김도한,류동춘,최동훈,류병순,송승구 한국화학공학회 2002 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.40 No.1

        본 연구는 무연탄으로 활성탄을 제조할 경우 성형이 세공특성에 미치는 효과를 고찰한 것이다. 3가지 다른 방법으로 활성탄(파쇄형 활성탄, 압축성형 활성탄, 압축성형 활성탄)을 제조하여 특성을 비교했다. 이중에서 25%의 콜타르와 7%의 물을 혼합하여 압출 성형한 활성탄이 비표면적, 세공부피, 그리고 경도에서 가장 좋은 물성을 나타내었다. 압축 및 압출 성형체의 표면은 무연탄 원탄과는 달리 무연탄 분말이 바인더와 혼합되어 있으므로 매우 거친 상태를 나타내었고, 활성화시 거칠게 형성되어 있는 입자사이로 활성화제인 수증기가 쉽게 침투하여 많은 세공을 형성시킴을 알 수 있었다. 이렇게 형성된 세공을 통해 수증기 활성화반응이 잘 일어나 직경 3,000-50,000Å 범위의 거대세공이 발달하였다. 파쇄형 활성탄에 비해 약 2배의 거대세공(macropore)부피를 가지고 있었으며 경도도 30%에서 95% 이상으로 상승되었다. This study was to investigate the effect of granulation process on pore characteristics in manufacturing anthracitebased activated carbons. The activated carbons were made by three different methods to compare characteristics: the crushed activated carbon, the compressed activated carbon, and the extruded activated carbon. Among these activated carbons, the extruded activated carbon using a binder that consists of 25% coal tar and 7% water showed the best characteristics in specific surface area, pore volume, and hardness. Since the compressed and the extruded substances had coarser surfaces than a raw material, steam could penetrate easily through particles and produce macropores especially in the diameter ranges of 3,000-50,000Å during activation process. The extruded activated carbon showed about twice more macropore volumes than the crushed activated carbon and the hardness was increased from 30% up to 95%.

      • 유산균의 배양 및 건조조건에 관한 연구 : 제1보 유산균의 배양조건에 관한 연구 partⅠ.Optimum Cultural Conditions of Lactic Acid Bacteria

        신원철,윤주천,김갑수,최승락,이근억,유주현 江原大學校 産業科學硏究所 1981 産業技術硏究 Vol.1 No.-

        In order to use as a starter after drying, optimum growth conditions of Str. thermophilus and L. bulgaricus were investigated. The results were as follows. The growth of Str. thermophilus was reached at stationary phase after 12 hours incubation and L. bulgaricus was after 6 hours. Str. thermophilus was grown well, in the medium containing 10% of skim milk, 2%5 of sucrose, 0.1% of KCl and 0.015%(v./v.) of soy-sauce. In the case of L. bulgaricus, 10% of skim milk, 2% of glucose, 0.5% of bacto-peptone, 0.1% of K₂HPO₄ and 0.02% of pyridoxine was suitable for the maximum growth.

      • KCI등재

        수증기 활성화법으로 제조된 활성탄의 탄화온도에 따른 세공구조 고찰

        이송우,나영수,김도한,최동훈,류동춘,송승군 한국환경과학회 2000 한국환경과학회지 Vol.9 No.4

        Activated carbons were prepared from Korean coal by steam activation in this study. The variation of pore structure of the activated carbons were investigated according to different carbonization temperatures. Yield, surface area, pore volume and pore structure of this activated carbon were compared with those of activated carbon prepared without carbonization. The investigated carbonization temperature ranged from 700℃ to 1,000℃. Carbonization was carried out in nitrogen atmosphere for 70 minutes and activation was performed by steam at 950℃ for 210 minutes. Surface area and pore volume of the resulting activated carbons increased with carbonization temperature. Also pore volume increased by 20% compared to the activated carbon without carbonization. Especially, in mesopore region, the activated carbon carbonized at 900℃ had more pores by 60% than that of activated carbon carbonized at other temperature.

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