http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Da Hyeon Choi,Jue-Yeon Lee,Jae-Hwan Nam,Yang-Hoon Kim,Yoon Jeong Park,Yoon Shin Park 대한독성 유전단백체 학회 2019 Molecular & cellular toxicology Vol.15 No.3
Backgrounds: Hepatocellular carcinoma (HCC) is the fifth most common cancer worldwide. However, little is known about the molecular mechanisms of HCC development, progression, or effective therapeutic approaches. Recently, granulin-epithelin precursor (GEP) has been suggested as a novel factor that can control HCC cell proliferation. Thus, we aimed to develop new cell-permeable and stable genetic agents that can control GEP expression in HepG2 cells. Methods: Small interfering RNA-GEP (GEP siRNA) was used for the development of therapeutic agents for HCC treatment. GEP siRNA was first modified by adding polyethylene glycol (PEG) at the terminus. Arginine- rich peptide (ARP) was chemically conjugated with PEGylated GEP siRNA. Chemical conjugates of GEP siRNA-PEG-ARP were evaluated for cytotoxicity and bioavailability, including stability against DNase I enzymatic activity and GSH. The conjugate was further evaluated for intracellular distribution and cell proliferation effects. Results: The optimal conjugation ratio of GEP siRNA to ARP was a 1:10 molar ratio, and stability was confirmed using DNase I and glutathione stability assays. Conjugates showed increased intracellular uptake and distribution in HepG2 cells. Interestingly, HepG2 cells treated with conjugates showed significantly decreased cell proliferation. Conclusion: These results provide insights into the importance of chemical modification of unstable genetic therapeutics for HCC treatment. Conjugation of PEGylated GEP siRNA with ARP may be a potential HCC therapeutic agent.
Choi Da Hyeon,Kang Seong Ki,Lee Kyeong Eun,Jung Jongsun,Kim Eun Ju,Kim Won-Ho,Kwon Young-Guen,Kim Kwang Pyo,Jo Inho,박윤신,Park Sang Ick 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.6
BACKGROUND: Beating cardiomyocyte regeneration therapies have revealed as alternative therapeutics for heart transplantation. Nonetheless, the importance of nitric oxide (NO) in cardiomyocyte regeneration has been widely suggested, little has been reported concerning endogenous NO during cardiomyocyte differentiation. METHODS: Here, we used P19CL6 cells and a Myocardiac infarction (MI) model to confirm NO-induced protein modification and its role in cardiac beating. Two tyrosine (Tyr) residues of b2-tubulin (Y106 and Y340) underwent nitrosylation (Tyr-NO) by endogenously generated NO during cardiomyocyte differentiation from pre-cardiomyocyte-like P19CL6 cells. RESULTS: Tyr-NO-b2-tubulin mediated the interaction with Stathmin, which promotes microtubule disassembly, and was prominently observed in spontaneously beating cell clusters and mouse embryonic heart (E11.5d). In myocardial infarction mice, Tyr-NO-b2-tubulin in transplanted cells was closely related with cardiac troponin-T expression with their functional recovery, reduced infarct size and thickened left ventricular wall. CONCLUSION: This is the first discovery of a new target molecule of NO, b2-tubulin, that can promote normal cardiac beating and cardiomyocyte regeneration. Taken together, we suggest therapeutic potential of Tyr-NO-b2-tubulin, for ischemic cardiomyocyte, which can reduce unexpected side effect of stem cell transplantation, arrhythmogenesis.
Choi Ho Keun,Kim Ga Yeon,Lee Ga Hee,su Jang Hee,Kang Da Hyeon,Lee Jin Pyo,Lee Dong-Ha 한국응용생명화학회 2024 Applied Biological Chemistry (Appl Biol Chem) Vol.67 No.-
Excessive clotting or abnormal platelet accumulation can lead to serious cardiovascular disorders such as atherosclerosis, stroke, and thrombosis. Therefore, it is imperative to identify compounds capable of controlling or impeding platelet aggregation to prevent the onset of cardiovascular diseases. Arteanoflavone, a compound extracted from Artemisia iwayomogi, has not garnered scientific recognition for its potential health benefits, recent studies have substantiated its anti-inflammatory, antioxidant, and anti-allergic properties. However, the precise mechanisms by which arteanoflavone influences platelet aggregation and blood clot formation have not been conclusively established. This research investigates arteanoflavone’s role in these processes, particularly in platelets induced by collagen. The study reveals a significant increase in the production of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) correlating with the administered dosage of arteanoflavone. Concurrently, a noticeable escalation is observed in substrates of cAMP-dependent kinase and cGMP-dependent kinase, specifically VASP and inositol 1,4,5-trisphosphate receptor (IP 3 R). Arteanoflavone demonstrates its ability to limit Ca 2+ movement in the dense tubular system through IP 3 R phosphorylation. Moreover, phosphorylated VASP inhibits the binding of fibrinogen to αIIb/β 3 , thus suppressing platelet activity. Arteanoflavone also stimulates the phosphorylation of PI3K/Akt, a protein linked to platelet granule release, and MAPK (ERK, JNK, and p38) protein, associated with both platelet granule release and TXA 2 production. Lastly, arteanoflavone impedes collagen-induced platelet aggregation and blood clot formation by inhibiting fibrin production in thrombin-induced platelets. Hence, it is suggested that arteanoflavone could be valuable as an agent that effectively deters platelet inhibition and blood clot formation through antiplatelet mechanisms. Excessive clotting or abnormal platelet accumulation can lead to serious cardiovascular disorders such as atherosclerosis, stroke, and thrombosis. Therefore, it is imperative to identify compounds capable of controlling or impeding platelet aggregation to prevent the onset of cardiovascular diseases. Arteanoflavone, a compound extracted from Artemisia iwayomogi, has not garnered scientific recognition for its potential health benefits, recent studies have substantiated its anti-inflammatory, antioxidant, and anti-allergic properties. However, the precise mechanisms by which arteanoflavone influences platelet aggregation and blood clot formation have not been conclusively established. This research investigates arteanoflavone’s role in these processes, particularly in platelets induced by collagen. The study reveals a significant increase in the production of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) correlating with the administered dosage of arteanoflavone. Concurrently, a noticeable escalation is observed in substrates of cAMP-dependent kinase and cGMP-dependent kinase, specifically VASP and inositol 1,4,5-trisphosphate receptor (IP3R). Arteanoflavone demonstrates its ability to limit Ca2+ movement in the dense tubular system through IP3R phosphorylation. Moreover, phosphorylated VASP inhibits the binding of fibrinogen to αIIb/β3, thus suppressing platelet activity. Arteanoflavone also stimulates the phosphorylation of PI3K/Akt, a protein linked to platelet granule release, and MAPK (ERK, JNK, and p38) protein, associated with both platelet granule release and TXA2 production. Lastly, arteanoflavone impedes collagen-induced platelet aggregation and blood clot formation by inhibiting fibrin production in thrombin-induced platelets. Hence, it is suggested that arteanoflavone could be valuable as an agent that effectively deters platelet inhibition and blood clot formation through antiplatelet mechanisms.
Nitrogen Stress가 적양배추(Brassica oleracea)의 callus분화 및 Anthocyanin 함량변화와 항산화 활성에 미치는 효과
예다인,김혜림,김효정,최문정,김초록,김영지,이지현,구민성,정 강현,이인순,문혜연 대구대학교 산업기술연구소 2012 産業技術硏究 Vol.23 No.1
In order to study on the effects of antioxidant activity, changing concentration of anthocyanin and callus formation of Brassica oleracea by nitrogen stress experimented callus culture in MS medium with 0.1 ㎎/ℓ IAA, 0.2 ㎎/ℓ kinetin and 0∼5 mM KNO3. As medium showed differentiation of callus and the pigmental deposit like red color inside callus tissue. We extracted functional material from natural leaf and callus tissue of Brassica oleracea to use 70% ethanol as solvent and those extract used to determine antioxidant activity as 1, 1-diphenyl-2-picryl-hydrozyl (DPPH) radicals scavenging effect and nitric oxide radical scavenging effect as well as the concentration of total phenol and flavonoid. Additionally, we measured the concentration of anthocyanin and catalase activity from natural and callus tissue. In result, M1 had over 35% anthocyanin concentration, 1.7 times catalase activity and 20% DPPH activity compared with control. MC, M2 and M3 also showed 50% DPPH activity compared with positive control, BHT. However, more natural tissue than callus tissue cultured with nitrogen source had high effect on nitric oxide radical scavenging and the concentration of phenol compound, flavonoid and vitamin C. As a result, nitrogen source brought hypersensitivity in callus tissue of Brassica oleracea, so this reaction only induced to change color of callus tissue, pigment deposit for culture and also increase anthocyanin concentration, DPPH activity. Therefore, natural tissue of Brassica oleracea is good food for anticancer and detoxification and it has high concentration of functional compound.
Antioxidant and anti-inflammatory activity evaluation of 20 kinds of native plants in China
Hyeon-Ju Lee,Sun-Yeop Lee,Min Hong,Joon-Hee Han,Da-Hye Choi,Liu Qing,Tae-Hyung Kwon,Soo-Ung Lee 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
In order to search for functional materials from natural products, 20 kinds of native Chinese food were received from the Green Food Research Institute in Jilin Province, China, and their antioxidant and anti-inflammatory activities were evaluated. For each extract, hot water and ethanol extracts were prepared and used for testing. As a result of measuring the antioxidant activity of each extract, At 400 μg/mL concentration, the hot water extract of Hovenia acerba Lindl. exhibited the highest antioxidant activity with DPPH radical scavenging activity (89.34±0.11%), ABTS radical scavenging (98.02±0.12%) and reducing power (0.489±0.01 OD value), and The total polyphenol content was the highest in the hot water extract of Rubus idaeus L. (182.38±3.43 mg/g). As a result of measuring the effect on RAW 264.7 cells induced by LPS treatment for anti-inflammatory activity evaluation, the highest NO production inhibitory activity was measured at the concentration of 200 μg/mL of hot water extract of Rubus idaeus L. ( 67.1 ± 8.2% of LPS), it also showed the highest activity in the inhibition of mRNA expression of COX-2, iNOS and IL6.