Antioxidant and cytoprotective effects of morin against hydrogen peroxide-induced oxidative stress are associated with the induction of Nrf-2-mediated HO-1 expression in V79-4 Chinese hamster lung fibroblasts

Lee, Moon Hee,Cha, Hee-Jae,Choi, Eun Ok,Han, Min Ho,Kim, Sung Ok,Kim, Gi-Young,Hong, Su Hyun,Park, Cheol,Moon, Sung-Kwon,Jeong, Soon-Jeong,Jeong, Moon-Jin,Kim, Wun-Jae,Choi, Yung Hyun Spandidos Publications 2017 International journal of molecular medicine Vol.39 No.3

<P>Natural phytochemicals of plant origin, including flavonoids, have been found to be potent antioxidants providing beneficial effects against oxidative stress-related diseases. The present study was carried out to investigate the antioxidant properties of morin, a flavonoid originally isolated from the flowering plants of the Moraceae family. Superoxide dismutase (SOD)-like activity and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(center dot+)) radical scavenging activity were determined. We also investigated the cytoprotective effects of morin against hydrogen peroxide (H2O2)-induced DNA damage and apoptosis in V79-4 Chinese hamster lung fibroblasts. Our results demonstrated that morin had strong scavenging effects against ABTS' radicals with enhanced SOD activity, which varied in a dose-dependent manner. Morin was found to reduce H2O2-induced intracellular reactive oxygen species generation and nuclear DNA damage, and it recovered cell viability damaged by H2O2 via inhibition of mitochondrial dysfunction-mediated apoptosis. Notably, the treatment of V79-4 cells with morin markedly enhanced the expression of heme oxygenase-1 (HO-1) but not quinone oxidoreductase-1, which was associated with the increased expression and phosphorylation of nuclear factor-erythroid 2-related factor 2 (Nrf2) and the downregulation of Kelch-like ECH-associated protein 1 expression. Based on our findings, we conclude that morin effectively ameliorated oxidative stress-induced DNA damage through intrinsic free radical scavenging activity and activation of the Nrf2/HO-1 pathway.</P>

단클론성 IgG 침착을 동반한 증식성 사구체 신염 1예

차정민,정덕은,송주흥,안선호 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.2

Proliferative glomerulonephritis with monoclonal IgG deposits is a novel phenotype of glomerular injury with the diagnostic criteria of (1) the presence of glomerular monoclonal IgG deposits restricted to a single IgG subclass and a single light chain isotype, associated with endocapillary proliferative, membranoproliferative, or membranous features; (2) the presence of granular("immune complex type") deposits by electron microscopy; and (3) the absence of clinical and laboratory evidence of cryoglobulinemia. We report a case of 65-year-old man with generalized edema who was diagnosed as proliferative glomerulonephritis with monoclonal IgG deposits by renal biopsy.

사회적 구성주의 학습이론에 기초한 예비 교사의 온라인 토론 분석

차정호,노태희 한국컴퓨터교육학회 2005 컴퓨터교육학회 논문지 Vol.8 No.1

예비 과학교사의 반성적 사고 향상을 위한 웹기반 포트폴리오 시스템의 개발

차정호 대구대학교 사범대학 부설 교육연구소 2009 敎育硏究 Vol.4 No.2

한국 전자화페산업의 성장전략에 관한 연구

박정호,차순권 군산대학교 지역개발연구소 2000 地域開發硏究 Vol.12 No.-

Today, world had unfolded the international information transaction actively due to dramatic development of computer and information communication technology, which can overcome time and space limitation. In the above situation, the commercial transaction, also is being changed not only commercial exchange way but payment method from traditional commerce to electronic commerce. And the demand necessity of Digital-money use is increasing in the world base as an unavoidable new payment method. Therefore, korea, also needs to prepare certain strategy to increase wide use of Digital-money and improve competitiveness of Digital-money industry. Thus, this study analyzed the strength and weaken of korea's Digital-money industry and groped for infrastructure strengthening strategy of korea to achieve above purpose. This study, for the above research, first contemplated the world Digital-money industry trend. Digital-money industry of U.S.A, Europe and Asia has some difference in acceptance way of Digital-money. U.S.A has some interest to open up ICcard type Digital-money by supporting of Visa and Marster card, however it has more interest in developing of Network type Digital-money for Internet commerce. On the other hand, Europe has more interest ICcard type Digital-purse then Network type Digital-money except Eu-Net which is Internet connection service company and Digicash of the of Netherlands. So, Europe has more advanced technology than U.S.A in ICcard part. With recognizing of avove current world Digital-money development situation, korea Digital-money industry, also, must accomplish more intensive investment and research development.

한 정신병원에서의 강박 처치에 대한 입원 환자 및 병원 직원의 지각의 차이

채정호,차성조,함웅,이규항,이정균 大韓神經精神醫學會 1996 신경정신의학 Vol.35 No.5

한 정신병원에서 강박처치의 적응증, 합병증, 대체법, 감정적 영향, 적당한 처치 시간, 처치의 효능과 필요성등을 병원에 입원 중인 환자들과 직원들에게 설문조사를 하여 양 집단간에 차이가 있는 지를 알아봄으로써 환자들과 직원들간에 있는 강박처치에 대한 지각 차이를 조사하고 나아가 강박처치 시행의 규준을 확립하기 위한 기본 자를 제시하기 위하여 본 연구를 시행하였다. 강박처치의 적응증에 대해서 입원환자들은 대부분의 항목에 대해 부정적으로 보았고 단지 타환자에 대한 신체적 공격에 대해서만 다소 긍정적인 반응을 보인 반면 직원들의 경우 자해, 타환자에 대한 신체적 공격, 치료진에 대한 공격 등에 높은 응답율을 보였다. 강박처치의 부작용에 대한 지각은 입원환자의 경우 정신상태의 악화, 자해의 증가 등에 대해 보다 민감하게 반응 하였고, 실제로 경험한 부작용에 있어서는 직원들의 경우 피부박탈을 더 강조한 반면 환자군에서는 호흡곤란, 정신 상태의 악화 등을 강조하였다. 강제 처치에 대한 대체치료 방법으로는 환자군에서 면담을 중요시한 반면 직원군에서는 격리실의 이용을 강조하였다. 신체억제와 고용량의 정온화를 비교한 것은 환자군에서 신체억제가 더 효과적이라고 하였다. 신체억제 동안 느끼는 감정에 대한 지각에서 환자군은 슬픔을 가장 많이 보고하였고, 직원군은 적개심을 가장 많이 느낄 것으로 추정하였다. 신체억제의 시간에 대해서는 환자군이 긴 것으로 지각하고 있으며, 이상적인 신체억제의 시간은 환자군이 짧은 것이 좋다고 생각하고 있었다. 본 연구 결과 정신과 입원환자들과 병원직원들은 강박처치에 대하여 유의한 지각의 차이가 있다는 것을 알았으며 정신병원내에서 강제처치를 실시할 때에는 명확한 강박처치 시행에 대한 규준에 따라야 할 것임을 확인할 수 있었다. Objectives : The purpose of this study was to examine different perceptions of physical restraint and their nature between psychiatric inpatients and hospital staffs in a mental hospital and to provide baseline data for establishing the practicing standard of this therapeutic maneuver. Methods : Two hundred and two psychiatric inpatients who experienced physical restraint and 103 hospital staffs who frequently used this maneurer were surveyed with a questionnaire regarding indications, complications, alternative methods, emotional influences, duration of treatment and subjective effects of restraint. Results : Regarding indications of physical restraint, the inpatient group had negative viewpoint on the most items except "physical assault to other patients". Regarding the perception about possible complications of this treatment, the inpatient group had more concerns about "deterioration of mental status" and "increasing risk of self-injury". For experienced complications, staffs stressed "skin abrasion " while inpatients believed of "respiratory difficulties" and "deterioration of mental status" more frequently. As alternative methods of physical restraint, "increasing frequency of interview" was suggested by the inpatient group and "using seclusion room" was favored by the staff group. The inpatient group had an impression that physical restraint was more effective than high-dose neuroleptic tranquilization. For suspected emotion during this procedure, the inpatient group reported "sorrow" most frequently while the staff group presumed that "hostility" might be most frequent. Inpatients estimated that the average duration of physical restraint was longer than staffs did. Ideal duration of physical restraint was shorter in the inpatient group. Conclusion : These results suggest that there are definite differences in the perception of physical restraint between psychiatric inpatients and hospital staffs. Formal practing guideline for restraint should be set up with careful attention for these differences to maximize the therapeutic effect of this maneuver.

신사구체 경변증을 나타내는 FGS/NgaKist mice 간장에서 사이토크롬 P4501A1/2 단백질 발현

정규식,현병화,이철호,박상준,최양규,류시윤,이차수,민병길 대한해부학회 1997 Anatomy & Cell Biology Vol.30 No.2

韓國 自動車工業 國際競爭力에 관한 實證的 硏究

박정호,차순권 군산대학교 지역개발연구소 1998 地域開發硏究 Vol.10 No.-

This paper investigates the empirical international competitiveness on the Korea Automobile Industry by using the five theoretical measurement methods which are micro international competitiveness fountainhead assessment, revealed comparative advantage index, revealed comparative advantage index, relative market intensity, and simple market share ratio change way. After that, this study analyzes the result of international competitiveness of the Korea Automobile Industry compared with another industrialized countries in same field. For the measurement of the source of international competitiveness concerning Korea Automobile Industry, this survey selects twelfth variables such as productivity, product quality level, product style creation capability, sales promotion capability, brand reputation, abroad market research capability, distribution cost, new product development capability, materialㆍpart product self-suffi- ciency, production technology, production equipment level, product improvement skill. Finally, this report proposes several solution or overcome strategies that to improve Korea Automobile Industry's competitiveness to survive and develope its boundary in unlimited global competition environment.

과학 전자교과서의 학습동기 분석 : 중학교 1학년 화학영역을 중심으로

류주희,차정호,김인환 대구대학교 사범대학 부설 교육연구소 2008 敎育硏究 Vol.3 No.2

본 연구에서는 과학 전자교과서의 동기유발전략을 분석하였다. 분석에는 Keller의 ARCS 동기이론이 사용되었다. 에듀넷의 중학교 1학년 화학 내용을 선택하였으며, 두 명의 연구자에 의해 동기 유발과 동기 유지 측면에서 분석되었다. 분석자간 일치도는 90%이상이었다. 연구 결과, 각 동기 전략이나 학습단계에 대한 차이는 없는 것으로 나타났다. 가장 많이 사용된 전략은 “사진의 패턴이나 글자를 돋보이게 하거나 거꾸로 나타내기”, “점진적으로 드러내기를 통한 교수 조각 맞추기”, “제목이나 키워드에 밑줄 긋기, 이탤릭체 또는 큰 글자크기를 사용하기”였다. “학습자의 집중력을 빼앗는 요소”는 가장 적은 빈도를 나타내었다. 학습의 마지막 단계에서는 전략 사용 빈도가 학습의 처음 단계에 비하여 상대적으로 낮았다. In this study, learning motivation strategies of the science e-textbooks, were analyzed. Keller's ARCS model was used for analysis. The 7th-grade chemistry contents ware selected from the EDUNET (edunet4u.net), then analyzed in aspect of gaining and sustaining attention by two researchers. Intercoder agreement of the analysis was over 90%. The results indicated that the frequencies of each motivation strategies were not different by learning stages. The most frequent strategies were "use inverse and flash in text and patterns in pictures as attention getters", "keep instructional segments relatively short with progressive disclosure", and "use underlines, italics, or bigger font sizes for the headings or key words". "Avoid disfunctional attention-getting effects such as a flashing word that distracts learners' concentration" was the lowest frequent strategy. At the end stage of learning, frequencies of the strategies were relatively low than those of early stage of learning. Educational implications are discussed.

한국의 중등 과학 교육과정의 실태와 개선 방안

임성민,차정호 대구대학교 2005 대구대학교 학술논문집 Vol.1 No.1

현대 사회에서 국가의 생존과 번영을 위해 우수한 잠재적 과학기술인력의 양성 뿐 만 아니라 전국민의 바른 과학소양 함양을 위해서 초중등 과학교육은 국가사회적으로 중요한 과제이다. 세계적으로 초중등 과학교육의 중요성을 인식하고 다양한 연구와 실천 및 정책적인 지원이 계속되고 있다. 이러한 맥락에서 한 나라의 초중등 과학교육의 목표와 내용, 방법 및 평가 등 전반적인 기준에 되는 과학교육과정은 매우 중요하다. 이에 따라 과학교육과정에 대한 철저한 연구와 분석이 필요하며, 이를 바탕으로 미래 사회에서 최적의 과학교육과정을 도출하고 실천하는 것이 중요하다. 이 논문에서는 한국의 제7차 과학과 교육과정의 특징을 교육과정 해설서를 중심으로 살펴보고, 운영 과정에서 발견된 문제와 이에 대한 해결 방안을 고찰하였다. 또한 한국의 과학교육과정의 변천, 제7차 과학과 교육과정의 특징, 현행 과학과 교육과정의 문제점과 개선방향, 제7차 과학과 교육과정의 운영상의 개선과제, 향후 과학과 교육과정 개편에 있어서 개선과제 등을 중심으로 논의하였다. 이상의 모든 과학교육과정의 분석과 개선과제 도출에 있어서 무엇보다 중요한 것은 과학교육 혁신의 주체가 바로 과학교사라는 점을 분명히 인식하여 이론적인 근거 뿐 아니라 현장교육의 실천을 고려한 과학교육과정에 대한 논의와 개발이 필요하다는 점이다. In modern society the school science education were emphasized for the survival and prosperity of one nation, which relates on the training of potential scientific manpower and the cultivation of public scientific literacy. Thus, it is necessary to explore the national curriculum including the aims, objectives, contents and assessments of school science education. In this study we analyzed the national science curriculum of Korea based on the commentary of the 7th National Curriculum of Korea. Also we investigated the transition of national science curriculum, the features, the controversial points, the direction of improvement of the 7th national science curriculum of Korea, and proposed the task in management of science curriculum and the considerations in development of future science curriculum. In conclusion, the authors pointed out that the science teachers are most important variables for they lead and execute the reform of school science education. So it is necessary to consider the practical aspects in behalf of a science teacher as well as theoretical researches in the analysis and development of science curriculum.