Enhanced Maturation and Function of Dendritic Cells Using Hydrogel Coated Plate and Antigen Electroporation

Son, Cheol-Hun,Bae, Jae-Ho,Shin, Dong Yeok,Lee, Hong-Rae,Choi, Yung Hyun,Yang, Kwangmo,Park, You-Soo Informa Healthcare 2013 Immunological investigations Vol.42 No.4

<P>Dendritic cells (DCs) are potent antigen-presenting cells that can be matured <I>in vitro</I> from immature dendritic cells (iDCs) in the presence of several biological agents such as cytokine cocktail, CD40L, TNF-a and antigen loading, which are necessary and achieved using various protocols, such as lipofection, passive pulse or electroporation. However, these DCs maturation protocols may cause with a significant loss of cells because of cellular attachment and spreading during culturing. Some biomaterials that influence adhesion and development of cells have been used in cell culture techniques, and it was thought that they might be applied on the culture of DCs. In this study, we used polyHEMA, which is a hydrogel coating biomaterial that prevents DCs from adherence, and investigated whether hydrogel coating affects the maturation of iDCs. The efficiency in the generation of mDCs was improved through hydrogel coating procedure and a dendritic cell maturation marker, CD83, was significantly increased in hydrogel-coated culture condition. The antigen-loaded mDCs from electroporation were further expressed the CD83. The mDCs generated in the hydrogel-coated culture condition showed more, longer and thicker dendrites, and produced more amounts of cytokines such as IL-12 and IFN-γ. Therefore, it was suggested that the hydrogel-coated culture condition could improve function of mDCs.<fn><P>Cheol-Hun Son and Jae-Ho Bae contributed equally to this work.</P></fn></P>

Ciprofloxacin 제제의 생물학적 동등성에 관한 연구

최철희,신재국,신완균,유호진,이경훈,정원석,장인진,신상구 대한화학요법학회 1991 대한화학요법학회지 Vol.9 No.1

국내 시판중인 ciprofloxacin 제제인 Citopcin®(250㎎ tablet)의 동등성을 검토하기 위해 제조원인 Bayer사의 Citopcin®(250㎎ tablet)를 기준제제로 하여 18명의 건강한 남성 피험자를 대상으로 500㎎ 1회 교차 경구 투여후 약동학적 성상을 분석비교한 결과는 다음과 같다. 시험제제의 생체 이용율의 지표인 AUC, C_(mao), T_(max) 및 MRT들의 평균치는 백분율차이에 있어 모든 기준제제 지표의 ± 20% 이내였으며, 이들 생체 이용율 지표들은 분산분석 검정에서 차이를 인지할 수 없었다.생체 이용율 지표들의 기준제제에 대한 백분율 90% 대칭 신뢰구간 검토시 모든 경수들에서 ± 20% 이내의 조건을 만족하였으며 AUC의 경우에는 대칭형 신뢰구간으로 변환시 95% 신뢰구간에서도 동등성의 조건을 만족하였다, 이상의 시험결과로 시험제제인 Citopcin®은 기준제제인 Ciprobay®와 생물학적 동등한 제제로 판단되었다. The pharmacokinetics of ciprofloxacin was studied for the evaluation of the bioequivalence of the generic ciproflocacin products. Two single doses of 500㎎ each of ciprofloxacin(Ciprobay® 250㎎ tablet as a reference compound and Citopcin® 250㎎ tablet s a test compound) were administered orally to ighteen male volunteers in a balanced, randomized crossover design. Pek plasma levels of ciprofloxacin were observed about 1 hour after the doses and the peak concentrations of both products were similar(Ciprobay®, 3.18㎍/㎖; Citopcin®, 2.89㎍/㎖). The values of other pharmacokinetic parameter of ciprofloxacin computed for Ciprobay® are presented in that order: AUC=13.53:12.47㎍·h/㎖, T_(max)=1.28:1.14 hours; MRT=5.30:5.08 hours; t_(1/2)β=3.65:3.47 hours. No satistically significant, differences were detected when AUC and the other parameters were compared with the method of ANOVA. Using the criteria of 90% confidence interval for the assessment of bioequivalence al the parameters were acceptable. The products were found to be equivalent on the premise that no significant difference was detected when the relevant pharmacokinetic parameters were compared, and the confidence limit analysis showed acceptable results.

중추에서 Prostaglandin계가 Renin-angiotensin System에 미치는 영향

최영태,김종승,문성호,오형균,김재훈,전제열,염철호,윤평진 朝鮮大學校 附設 醫學硏究所 1997 The Medical Journal of Chosun University Vol.22 No.1

Role of prostaglandins on the renin-angiotensin system in the central nervous system was examined in normotensive and 2-kidney, 1 clip (2K1C) hypertensive rats. The experiment was done under thiopental (50 ㎎/㎏, IP) anesthesia. Captopril and indomethacin were injected into the right lateral cerebral ventricle. Arterial blood pressure and heart rate were recorded from the femoral artery. Intracerebroventricular (ICV) captopril (1 ㎎) caused a decrease in mean arterial pressure in both normotensive and 2K1C hypertensive rats. The depressor response to captopril was more sensitive in hyper-tensive rats than in normotensive rats. Indomethacin treatment (ICV, 200 ㎎) altered the depressor response to captopril neither in normotensive nor in hypertensive rats. These results suggest that the cardiovascular effect of renin-angiotensin system in the central nervous system may not be mediated via prostaglandin systems in normotensive and 2KlC hypertensive rats.

지리 진동 히트파이프의 압력 및 온도 파형 해석

최재혁,윤두호,윤석훈 한국해양대학교 산업기술연구소 2002 연구논문집 Vol.19 No.-

Heat transfer characteristics of self-oscillating heat pipe were experimentally investigated for the effect of fill charge ratios and heat loads. The heat pipe used for this study is made of copper capillary, has 0.002m internal diameter, a 0.34m length in one turn and consists of 19 turns. Heating and cooling section each have a length of 70mm. Water was used as working fluid inside the heat pipe. As the experimental results, the self oscillating heat pipe was operated b self-exited oscillation and circulation of working fluid and the oscillation within the self-oscillating heat pipe assumed chaotic behavior.

LPC열의 상관 차원에 의한 화자인식

최성재,이헌규 경원전문대학 2000 論文集 Vol.22 No.-

The thesis is about the methods of speaker recognition by applying strange attractor of linear prediction coefficient series to speaker recognition. We have accomplished speaker recognition experiments by utilizing strange attractor of time series as well as of linear prediction coefficient series. The results were 94% recognition ratios. Therefore, the strange attractor of linear prediction coefficient series was more appropriate characterized parameter in increasing speaker recognition ratios.

Si의 변형퍼텐셜 폴라론계에 대한 싸이클로트론 흡수곡선

최상돈,이재훈,남인호 경북대학교 산업기술연구소 1987 産業技術硏究誌 Vol.15 No.-

Choi-Chung's cyclotron resonance lineshape formula is applied to a deformation potential polaron system in Si. The result is in qualitative agreement with the experimental result of Kido et al.

다중 특징의 반복 분석에 의한 퍼지 분류기

최재익,나철훈 木浦大學校 工業技術硏究所 1997 工業技術硏究誌 Vol.7 No.-

A fuzzy classifier which needs various analyses of features using genetic algorithms is proposed. The fuzzy classifier has a simple structure, which contains a classification part based on fuzzy logic theory and a rule generation part using genetic algorithms. The rule generation part determines optimal fuzzy membership functions and inclusion or exclusion of each feature in fuzzy classification rules. We analyzed recognition rate of a specific object, then added finer features repetitively if necessary to the object which has large misclassification rate. And we introduce repetitive analyses method for the minimum size of strings and population, and for the improvement of recognition rates. This classifier is applied to three examples of the classification of iris data, the discrimination of thyroid gland cancer cells and the recognition of confusing handwritten and printed numerals, each sample numeral is classified into one of the groups which are diveded according to the sample structure. The fuzzy classifier proposed in this paper has recognition rates of 98.67% for iris data, 98.25% for thyroid gland cancer cells and 96.3% for confusing handwritten and printed numerals.

특발성 좌심실빈맥(Idiopathic Left Ventricular Tachycardia)의 성공적인 전극도자절제술을 위해 단극전기도(Unipolar Electrogram)가 유용한가?

최기준,남기병,강덕현,홍명기,송재관,김재중,박성욱,박승정,박종훈,김유호 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.4

지역금융시장에서 지역농협의 역할에 대한 실증적 연구 : 부산경남지역을 중심으로 an Empirical Study

최진배,김태훈,민재현 한국경제지리학회 2004 한국경제지리학회지 Vol.7 No.3

지역농협은 협동조합적 금융기관이다. 그러나 지역경제의 금융제약을 완화하기에 적합한 조직구조와 특성을 지니고 있다. 특정지역에서 한정된 고객=조합원을 상대로 영업하고 있기 때문이다. 지금 그것은 심각한 정체성의 위기에 직면하고 있다. 그러나 변화하는 환경에 적응하려고 노력해오면서 지역금융기관으로서 위상을 상당히 구축한 것으로 보인다. 이 글은 지역농협이 지역의 영세기업과 서민가게를 주된 고객으로 하면서 이들의 금융제약을 완화해주는데 많은 기여를 하고 있으며, 나아가 은행권이 상대적으로 관심을 적게 기울이는 농촌지역금융시장에서 독점적인 지위를 누리고 있다는 점을 밝히고 있다. 이 글은 지역농협의 영업기반을 실증적으로 분석한 최초의 글이라 할 수 있다. 한편 이러한 연구결과는 지역농협을 지역금융기관으로 육성해야 할 필요성이 있다는 것을 보여준다. The national agricultural cooperative federation is a kind of credit unions. But because it operates for a small group of members in a specified areas we can say that it has suitable structures and characteristics appropriate to ease regional financial constraints. Now it is suffering identity crisis. But trying to adapt itself to new environments it is changing into a regional financial institute. This paper tries to show that national agricultural cooperative federation serves to ease the financial constraints of small firms and households and has monopoly powers in rural financial markets. This is the first paper that surveys the operational grounds of national agricultural cooperative federation. The results reveal that national agricultural cooperative federation needs to be developed as a regional financial institute.

CuO를 열확산시킨(Sr.Ca)TiO_(3) 입계층 커패시터의 전기적 특성

최운식,강재훈,오남석 대불대학교 2002 大佛大學校大學院 硏究論文集 Vol.- No.1

In this paper, the Sr_(1-x)Ca_(x)TiO_(3)(0≤x≤0.2)-based grain boundary layer ceramics were fabricated to measured dielectric properties with the sintering temperature and the thermal treatment time. The sintering temperature and time were 1420∼1520℃, 4hours, and the thermal treatment temperature and time of the specimen were 1150℃, 1, 2, 3,hours, respectively. The structural and the dielectric properties were investigated by SEM, X-ray, HP4194A and K6517. The average grain size was increased with increasing the sintering temperature, but decreased to 15㏖% with increasing content of Ca. X-ray diffraction analysis results showed that all specimens were the cubic structure, the main peaks were of Ca. The appropriate thermal treatment temperature and time of CuO to obtain dielectric properties of ε_(γ)>50000, tan δ<0.05 and ΔC<±10% were 1150℃ and 2hours, respectively.