식용 버섯의 조리방법에 따른 Jurkat 세포주 DNA 손상 보호 효과

조윤정,김경희,육홍선,Cho, Yun-Jeong,Kim, Kyoung-Hee,Yook, Hong-Sun 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.1

본 연구는 식용 버섯의 조리방법에 따른 항산화 생리활성의 평가를 위해 수행되었으며, 산화적 스트레스에 의한 DNA 손상 감소 효과를 통해 조리방법을 달리한 버섯 추출물의 유전독성학적 방호효과를 살펴보았다. Human lymphocyte에 조리방법을 달리한 3가지 버섯(느타리, 팽이, 표고)의 추출물을 처리하고, hydrogen peroxide($H_2O_2$)로 산화적 손상을 준 후, DNA 감소 효과를 Comet assay로 평가한 결과, 모든 시료군에서 산화적 손상에 의한 DNA 손상 감소 효과를 나타냈다. 3가지 버섯 모두 비조리군이 조리군보다 높은 효과를 나타냈는데, 이는 조리과정에 의한 페놀성 화합물의 감소로 인한 것으로 보이며, 조리군 중에서 볶기와 전이 비교적 낮은 DNA 손상 감소 효과를 나타낸 것은 조리 시 첨가되었던 대두유의 가열 산화에 의한 것으로 사료된다. 결론적으로, 조리된 버섯은 생버섯에 비해 산화적 스트레스에 의한 DNA 손상 감소효과가 낮으나, 양성 대조군과 비교하였을 때 손상을 유의적으로 감소시킨 것으로 나타났다. 또한, 본 연구에서 사용한 네 가지 조리법(굽기, 데치기, 볶기, 전) 중 DNA 손상 감소에 효과적인 조리법은 대두유를 사용하지 않은 굽기와 데치기인 것으로 판단된다. In this study, portective effect on DNA damage several mushrooms (Pleurotus ostreatus, Flammulina velutipes, Lentinula edodes) according to cooking methods was investigated using Comet assay. Three edible mushrooms were cooked by grilling, blanching, pan-frying, or by preparing 'Jeon' (traditional Korean pancake). Cells were incubated in medium with 4 kinds of samples for 48 h ($37^{\circ}C$) were further treated with hydrogen peroxide ($H_2O_2$) for 5 min as an oxidative stimulus. Oxidative damage was evaluated by single-cell gel electrophoresis (Comet assay) and quantified by tail DNA% (TD), tail length (TL), tail moment (TM). Though oxidative DNA damages expressed as TD, TL, TM of 4 cooked samples were higher than raw sample, which means lower protective activities, all samples including raw sample had significantly higher protective effects than the positive control (p<0.05). The protective effect on DNA damage of cooked samples decreased much more when soybean oil added, likely due to the thermal oxidation of oil during cooking. Although heat treatment could degrade protective effect on DNA damage of mushrooms, the cooked mushroom had significant effect on oxidative stress. In conclusion, grilling and blanching were the most advantageous cooking methods to protect oxidative DNA damage induced by $H_2O_2$.

갑상선 미세 유두암에서 종양 크기의 구분에 따른 임상병리학적 특징의 분석

조윤정,이동호,이상철,김세준,김정구,안창준,이관주,Yun-Jung Cho,M,D,Dong-Ho Lee,M,D,Sang-Chul Lee,M,D,Say-Jun Kim,M,D,Jung-Koo Kim,M,D,Chang-Joon Ahn,M,D,and Kwan-Ju Lee,M,D 대한갑상선-내분비외과학회 2010 The Koreran journal of Endocrine Surgery Vol.10 No.3

Purpose: Although the detected incidence of papillary thyroid microcarcinoma (PTMC) has increased with development of ultrasonography and fine-needle aspiration biopsy, the best treatment has not yet been established. Treatment decisions require information on many factors including lymph node metastasis, extrathyroidal extension, and bilaterality. With this aim, the present study analyzed clinicopathologic features of PTMC according to cut-off of tumor size. Methods: The clinicopathologic features of patients with PTMC between January 2007 and December 2009 were reviewed retrospectively from medical records. Patients were divided according to tumors lesser than or equal to cut-off (Group I) and tumors exceeding cut-off (Group II). Results: Both capsule invasion and lymphovascular invasion were significantly different at all cut-off diameters (5∼9 mm). Central node metastasis revealed a difference in all cut-off values except 8 mm. Extrathyroidal extension differed at all cut-off values except 5 mm. Bilaterality displayed a statistically significantdifference only at the 8 mm cut-off. Conclusion: A cut-off of 5 mm represents a safe value to discriminate less aggressive from aggressive treatment for PTMC. (Korean J Endocrine Surg 2010;10:152-156)

Depressive Mood and the Risk of Future Functional Decline in an Elderly Population

조윤정,홍영호,박형준,이지은,윤재문,신동욱,손기영,Be-Long Cho,김상혁 대한노인병학회 2017 Annals of geriatric medicine and research Vol.21 No.3

Background: As the elderly population increases in number, prediction and prevention of functional decline in this population are of great importance. This study evaluated whether depression in an elderly population could predict functional decline in activities of daily living and the components of instrumental activities of daily living 2 years after baseline. Methods: Data from the Korean Longitudinal Study of Aging panel, a national, representative sample of aging Koreans, were used. The subjects were the elderly aged 65 years or older who had no disabilities that hindered them from performing activities of daily living at baseline. Depression was measured using the Short-form (10-item) Center for Epidemiological Studies Depression Scale. Multivariable logistic regression analysis was performed to evaluate the effect of functional impairment according to baseline depression. Results: Of the 3,477 participants, 166 (4.8%) showed at least 1 impairment in the 7 activities of daily living (ADL) categories after 2 years. The participants with depression (scored 15 or higher on the depression scale) were more likely to have functional decline 2 years later (odds ratio, 1.82; 95% confidence interval, 1.23-2.71). All the components of the ADL had an increased tendency to develop functional decline (all odds ratios>1.00). Furthermore, negative items were significantly associated with functional decline (all p-values adjusted for trend<0.05). Conclusion: The participants with depression were more likely to have functional decline 2 years later. Such decline was observed in all types of ADL. Therefore, depression should be monitored and used as a predictor of functional decline in elderly adults.

점막하 침윤 조기위암 환자에서 VEGF-C와 COX-2 발현의 임상적 의의

조윤정,이정의,이관주,박조현,박승만,전해명,안창준,김정구,이동호,이상철,Cho, Yun-Jung,Lee, Jung-Uee,Lee, Kwan-Ju,Park, Cho-Hyun,Park, Seung-Man,Jeon, Hae-Myung,Ahn, Chang-Joon,Kim, Jeong-Goo,Lee, Dong-Ho,Lee, Sang-Chul 대한위암학회 2009 대한위암학회지 Vol.9 No.3

목적: Vascular endothelial growth factor (VEGF)-C와 -D 및 Cyclooxygenase (COX)-2는 위암에서 림프절 전이와 연관이 있다고 알려져 있다. 이에 저자들은 점막하 침윤 조기위암에서 VEGF-C와 -D 및 COX-2의 발현과 림프절 전이 등을 포함하는 다양한 임상병리학적 인자와의 관련성을 알아 보고자 하였다. 대상 및 방법: 1991년 1월부터 2007년 10월까지 본원에서 점막하 침윤 조기위암으로 수술을 시행 받은 85명의 환자를 대상으로 VEGF-C, -D 및 COX-2와 VEGF-C에 대한 면역 조직화학 염색을 시행하였다. 염색의 결과에 따라 환자군을 나누어 다양한 임상병리학적 인자와의 연관성을 조사하였고, 또 이 세 가지 인자들 상호 간의 연관 관계를 분석하였다. 결과: 전체 85명의 환자 중 16명이 림프절 전이가 있었다(18.8%). VEGF-C는 34.1% VEGF-D는 22.3% 그리고 COX-2는 37.6%가 양성으로 판정되었다. 이 중 VEGF-C와 COX-2 모두 림프절 전이와 유의한 상관관계를 보였고(P<0.001, P=0.023). VEGF-D와 연관성을 보이는 인자는 확인하지 못하였다. 또 VEGF-C와 COX-2의 발현은 밀접한 상관관계를 보였다(P=0.001). 결론: 점막하 침윤 조기위암에서 VEGF-C와 COX-2는 림프절 전이와 연관이 있고, 따라서 이 두 인자가 점막하 침윤 조기위암의 림프절 전이를 예측하는 인자로서의 가능성이 있다고 할 수 있겠다. Purpose: Lymph node metastasis is an important factor in determining prognosis and therapeutic options for early gastric cancer (EGC) patients. Vascular endothelial growth factor (VEGF)-C and D are known as lymphangiogenic factors, and cyclooxygenase (COX)-2 is thought to play a role in lymph node metastasis in gastric carcinoma. This study was designed to determine whether the expression of VEGF-C, VEGF-D, and COX-2 is associated with clinicopathologic factors, especially lymph node metastasis in EGCs invading the submucosa. Materials and Methods: Tissue samples were obtained from 85 Patients undergoing standard gastrectomy with lymph node dissection between 1991 and 2007 in the Department of Surgery of Daejeon St. Mary's Hospital in Daejeon, Korea. All patients were diagnosed with gastric cancers and submucosal invasion. We examined the expression of VEGF-C, VEGF-D, and COX-2 using immunohistochemical methods. Results: Of the 85 patients, 16 (18.8%) had lymph node metastasis. VEGF-C, VEGF-D, and COX-2 were positively expressed in 34.1% (29/85), 22.3% (19/85), and 37.6% (32/85) of the patients. VEGF-C and COX-2 expression was significantly correlated with lymph node metastasis (P<0.05). A positive correlation existed between VEGF-C and COX-2 expression (P< 0.001). Conclusion: VEGF-C and COX-2 expression is associated with lymph node metastasis in gastric cancer with submucosal invasion. VEGF-C and COX-2 may thus be predictive markers for lymph node metastasis in EGC patients with submucosal invasion.

출혈우세병변이 동반된 연령관련 황반변성 환자에서의 유리체강 내 라니비주맙 주입술의 효과

조윤정,박성표,Yoon Jung Cho,Sung Pyo Park 대한안과학회 2011 대한안과학회지 Vol.52 No.7

Purpose: To report the efficacy and safety of intravitreal ranibizumab monotherapy in patients with age-related macular degeneration with a predominantly hemorrhagic lesion. Methods: Nineteen eyes of 19 patients with submacular hemorrhage involving the fovea were treated with intravitreal ranibizumab (0.5 mg/0.05 ml) injections as needed. All patients completed at least 4 months of follow-up. Ophthalmologic examinations, fluorescence angiographic evaluations, optical coherence tomography (OCT) examination, and hemorrhage size were analyzed before the injections, and at 1,2,4,6, and 12 months follow-up. Results: The average VA and mean central retinal thickness (CRT) before ranibizumab treatment were 0.62 ± 0.39 log MAR and 335.76 ± 111.22 μm, respectively. Additionally, the VA and CRT four months after the initial injections were 0.54 ± 0.43 log MAR and 241.42 ± 107.55 μm, respectively. The mean size of the hemorrhage was significantly reduced from 2.87 ± 2.44 DA (disk areas) at baseline to 0.9 ± 1.28 DA at four month follow up. Conclusions: Intravitreal injection of ranibizumab is an effective treatment option for patients with age-related macular degeneration with a predominantly hemorrhagic lesion. J Korean Ophthalmol Soc 2011;52(7):838-845

변형된 광역학 요법으로 치료한 유방의 침윤성 관상암종의 맥락막 전이 1예

조윤정,박성표,Yoon Jung Cho,MD,Sung Pyo Park,MD 대한안과학회 2010 대한안과학회지 Vol.51 No.3

Purpose: To report a case of choroidal metastasis of breast cancer that was treated with modified photodynamic therapy. Case summary: A 45-year-old woman visited our clinic with blurred vision of the right eye, which began 1 month before. The patient previously suffered from a low back pain for 1 year. The best corrected visual acuity was 20/20 in both eyes. Fundus examination revealed an elevated yellowish mass-like lesion at the superonasal area in the right eye. Ultrasonography of the right eye showed a highly echogenic choroidal mass with moderate to high internal reflectivity. Fluorescein angiography showed hypofluorescence during the prearterial and arteriovenous phase, and well circumscribed hyperfluorescence during the venous phase. Radiologic examination was performed upon suspicion of metastasis. The examination revealed breast cancer with lung, spine and ovary metastasis. Subsequently, biopsy of the breast mass revealed an invasive ductal carcinoma. Based on these results, the patient was diagnosed with choroidal metastasis from breast carcinoma. The patient received systemic chemotherapy, and modified photodynamic therapy (PDT) was performed on the metastatic choroidal mass. Six days after modified PDT, the mass size was unchanged, and serous retinal detachment developed at the macula and inferior retina. However, 22days after treatment, the mass size markedly decreased and the serous retinal detachment was improved. J Korean Ophthalmol Soc 2010;51(3):458-462

병원 간호사의 이직의도에 대한 간호관리자의 리더십 효과: 체계적 문헌고찰 및 메타분석

조윤정(Cho, Yunjeong),정석희(Jeong, Seok Hee),김희선(Kim, Hee Sun),김영만(Kim, Young Man) 한국간호과학회 2022 Journal of Korean Academy of Nursing Vol.52 No.5

대학생들의 미래 삶의 목표가 학업 참여와 소진에 미치는 영향

조윤정(YoonJung Cho),심성옥(Sungok Serena Shim),황소리(Sori Hwang),Helsper,Addison 숭실대학교 영재교육연구소 2023 Global Creative Leader Vol.13 No.4

본 연구에서는 308명의 중국 의과대학 학생들을 대상으로 미래 삶의 목표와 학업 참여 및 소진 경험 간의 관계에서 자기결정적 동기의 매개효과를 살펴보았다. 경로모형 분석결과, 미래 삶의 목표(재정적 성공, 사회적 지위, 공동체적 목표)는 다양한 수준의 자기 결정적 동기와 관련이 있으며, 결과적으로 학업 참여와 소진에 영향을 미치는 것으로 나타났다. 재정적 목표는 통제된 동기를 매개로 학업적 소진을 유의미하게 증가시켰다. 사회적 지위 목표는 자율적 동기를 높여 학업 참여에 긍정적인 영향을 미치지만, 통제된 동기를 매개로 소진을 증가시키는 것으로 나타났다. 반면, 공동체적 목표는 자율적 동기를 높여 학업 참여도를 높이는 동시에 감소된 무동기를 매개로 소진 경험을 줄이는 것으로 나타났다. 이러한 연구결과는 학생들의 현재 학업 동기와 참여도를 이해하는 데 있어 학생들의 미래 인생 목표가 중요한 역할을 한다는 점을 강조한다. The current study investigated the mediating role of self-determined motivation in the relationship between future life goals and academic engagement and burnout of 308 Chinese Medical University students. The path analysis results revealed that distinct future life goals (financial success, social status, and communal goals) corresponded to distinct levels of self-determined motivation. These motivational levels subsequently impacted both academic engagement and burnout. Financial goals predicted heightened burnout through a pathway of controlled motivation. Social status goals, while positively influencing academic engagement via increased autonomous motivation, also contributed to elevated burnout through controlled motivation. On the contrary, communal goals amplified academic engagement through augmented autonomous motivation while simultaneously reducing burnout by diminishing amotivation. These findings emphasize the significant role of students’ future life goals in understanding their current academic motivation and engagement.

고소작업 사고 시나리오 기반 웨어러블 응용 HSE 시스템 안전관리 방안

조윤정(YunJeong Cho),임기창(KiChang Im),임동선(DongSun Lim),박정호(JeongHo Park),김종면(JongMyon Kim) 인문사회과학기술융합학회 2018 예술인문사회융합멀티미디어논문지 Vol.8 No.5

본 논문에서는 조선해양 작업장에서 발생하는 중대재해를 줄이고 체계적인 안전관리를 위하여 ETA(event tree analysis)기반 시나리오 도출 및 ICT 기술접목을 통한 안전관리 구축방안을 제안한다. 안전보건공단과 (구)국민안전처의 통계결과 조선해양 관련 중대재해 중 가장 많이 발생하는 사고유형은 떨어짐이고, 주요사고원인은 안전대 미착용 및 안전대 고리 미체결이다. 이 문제를 해결하기 위해 ETA기반의 시나리오를 작성하여 안전사항에 따른 결과를 도출하고 이 결과를 바탕으로 사고예방을 위한 ICT 기술접목으로 해결방안을 제시한다. ETA 기반 시나리오 도출 및 ICT 기술접목을 통해 제안한 해결방안으로는 안전대 및 안전모 착용여부 감지시스템, 안전대 고리 체결여부 감지시스템, 안전거리 측정을 위한 걸이설비 측정시스템이다. 안전사항별 시스템을 통해 작업자의 떨어짐 위험을 줄여 사망확률을 낮출 수 있다. 제안한 방안을 통해 사고를 예방함으로써 조선해양 분야의 중대재해를 줄이고 체계적인 안전관리를 도모한다. This paper proposes a safety management method that extracts ETA (event tree analysis) based scenario and combines ICT technology to reduce serious disasters occurring workplace for shipbuilding and offshore plant. The statistics of Safety and Health Agency and (previous)Ministry of Public Safety and Security show that the most frequent accident among the serious disasters related to shipbuilding and offshore plant is falling. The main cause of accidents is absence of a safety belt and safety belt ring. To solve these problems, we create ETA based scenarios to derive results based on safety considerations. Based on these results, we propose a solution by applying ICT technology for accident prevention. Deriving ETA based scenarios and ICT technology, the proposed solutions include a system for detecting the wearing of safety belts and safety helmets, a system for detecting whether or not the safety belts are connected, and a hook system for measuring safety distances. These safety related systems can reduce the probability of death of workers. By preventing accidents using the proposed method, we can reduce serious disasters in shipbuilding and offshore plant and establish systematic safety management.

효율적인 모니터 사용을 위한 화면 배치 시스템 구현 : 와이드 모니터 사용자를 위한 화면 구성 시스템 SquareSpace

조윤정(Yun-jeong Cho),안동언(Dong Un An) 한국HCI학회 2009 한국HCI학회 학술대회 Vol.2009 No.2

최근 대형, 와이드 모니터 사용이 일반화되면서 모니터의 작업 공간을 효율적으로 사용하고자 하는 사용자들이 늘어나고 있다. 하나의 윈도우만을 띄워놓고 작업하던 일반적인 사용 패턴에서 여러 개의 창을 한꺼번에 놓고 작업하는 사용 패턴으로 변화하고 있다. 본 논문에서는 사용자가 모니터의 작업공간을 효율적으로 사용할 수 있도록 윈도우 배치를 돕는 시스템을 구현하였다. 일반 유틸리티와 달리 미리보기 화면을 통한 배치가 이루어지기 때문에 사용자가 쉽고 간편하게 작업환경의 구성과 변환할 수 있다. 앞으로 대형, 와이드 모니터의 수요에 발맞춰 윈도우를 효율적으로 이용하고자 하는 사용자의 욕구를 만족시키고 일일이 윈도우를 조절해야하는 불편함을 해소시켜 줄 것이다. Recently, uses of a large, wide screen monitors are general, so people that efficiently use work area of monitor is encreasing. Use pattern of monitor is being changed just using one window to many windows at a time. In this paper, I will explain develop windows placement system for efficient use monitor. It is different from other general utility about windows placement system because users can easily change windows placement to use preview window. The future, a large, wide screen monitors to keep pace with the demand to take advantage of the window to satisfy the needs of the user to manually adjust the window to the inconvenience that will be resolved.