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감마선 조사된 홍삼분말의 항산화 효과 및 유전독성학적 안전성
육홍선,김성애,조성기,변명우 한국식품위생안전성학회 1996 한국식품위생안전성학회지 Vol.11 No.1
The yields of solvent fractions of irradiated red ginseng powder were increased in the order of petroleum ether(PE)$lt;diethyl ether(DE)$lt;ethyl acetate(EA)$lt;n-butanol (BU)$lt;aqueous fraction(AQ), and did not show any changes in fraction yields by irradiation dose levels. Inhibition activities of lipid peroxide formation were increased in the order of AQ$lt;BU $lt;PE$lt;EA$lt;DE. Inhibition activities of malonaldehyde formation were increased in the order of AQ≤BU$lt;EA$lt;PE$lt;DE. AQ fraction showed little effects on the antioxidative activity and all the activities of the samples did not changed by gamma irradiation. The reverse mutation assay using Salmonella typhunurium (TA98, TA100 and TA102) demonstrated that the nonirradiated and irradiated red ginseng powder extract did not have mutagenic activity (presence of S9 mix or not). The chromosomal aberration test in mammalian animal cell (Chinese hamster lung fibroblast, CHL) showed no significant increase in incidence of structural and numerical aberrations, comparing gamma-irradiated red ginseng powder extracts to nonirradiated red ginseng powder extract in the concentration of the sample producing cytotoxicity(presence of S9 mix or not). Therefore, gamma-irradiatied red ginseng powder (upto 10 kGy) could be safe on the genotoxic point of view.
신선초 분말의 위생화를 위한 오존처리와 감마선 조사와의 비교 효과
육홍선,변명우,김정옥,김종군,이현자 한국식품위생안전성학회 1997 한국식품위생안전성학회지 Vol.12 No.2
For the purpose of improving hygienic quality of dried-Angelica Keiskei Koidz powder, the effects of ozone treatment and gamma irradiation on the microbial decontamination and physicochemical properties were investigated. Gamma irradiation at 5 to 7.5 kGy resulted in sterilizing total aerobic bacteria, molds and coliforms below detective levels, while ozone treatment for 8 hours up to 18 ppm did not sufficiently eliminate the total aerobic bacteria of the sample. The physicochemical properties of the sample were not changed by gamma irradiation up to 7.5 kGy, whereas, ozone treatment caused remarkable changes in pH, TBA value, chlorophyll, carotenoid and fatty acid compositions. Therefore, this investigation demonstrated conclusively that gamma irradiation was more effective than ozone treatment for decontaminating and sterilizing the dried-Angelica Keiskei Koidz powder, with minimal effect on the physicochemical properties analyzed.
우렁쉥이 껍질로부터 정제된 섬유소 첨가 어묵의 품질특성
육홍선(Hong-Sun Yook),이주운(Ju-Woon Lee),이현자(Hyun-Ja Lee),차보숙(Bo-Sook Cha),이승용(Seung-Yong Lee),변명우(Myung-Woo Byun) 한국식품영양과학회 2000 한국식품영양과학회지 Vol.29 No.4
우렁쉥이 껍질로부터 정제한 섬유소 슬러리를 각각 2.5%, 5% 및 10% 첨가하여 기능성 어묵을 제조하고 기계적 물성, 전단력, 색도 및 관능 평가를 수행하여 제품의 품질을 평가하였다. 기계적 조직감 측정 결과 섬유소를 첨가할수록 견고도, 부착성, 껌성, 씹힘성 및 전단력이 증가되었고, 수분활성도와 색도는 유의적인 차이가 없었다. 관능검사 결과 모든 시료의 색택, 조직감 및 전반적 기호도에서 유의적인 차이가 없었으나 5% 첨가구를 선호하는 것으로 나타났다. Fish paste was prepared to enhance physiological functions by adding 2.5, 5 and 10% dietary fiber isolated from ascidian (Halocynthia roretzi) tunic. Hardness, adhesiveness, gumminess, chewiness and shear force of the fish paste were increased with addition of the dietary fiber. Water activity and Hunter's color values of the fish paste were not significantly changed by addition of the dietary fiber. Results of sensory evaluation indicated that no difference was observed in color, texture and overall acceptance (p<0.05). However, the fish paste with 5% dietary fiber scored the highest and was generally preferred by sensory panels.
우렁쉥이 껍질로부터 정제한 식이섬유 급여 Rat의 영양학적 특성 및 혈청지질함량의 변화
육홍선(Hong-Sun Yook),김정옥(Jung-Ok Kim),최정미(Joung-Mi Choi),김동호(Dong-Ho Kim),조성기(Sung-Ki Cho),변명우(Myung-Woo Byun) 한국식품영양과학회 2003 한국식품영양과학회지 Vol.32 No.3
우렁쉥이 추출 불용성 식이섬유를 5%, 10%, 20% 첨가한 식이 후 실험쥐 집단의 영양생리학적 특성변화를 조사하였다. Cellulose 급여에 따른 총 식이량에는 차이가 없었으나 식이 중 cellulose의 함량이 높아짐에 따라 체중은 감소하였으며 식이효율의 조절, 배변량 증가, 변의 수분 증가, 식이의장 통과시간 단축, 장 길이의 증가 등의 유의적인 효과를 나타내었다. 우렁쉥이 식이섬유를 급여한 실험쥐의 혈청분석 결과 total cholesterol, LDL-cholesterol, 중성지질 및 인지질, 혈당은 식이섬유의 함량이 높아짐에 따라 감소하였으며 HDL-cholesterol은 식이섬유의 함량이 높아짐에 따라 증가하였다. 따라서 우렁쉥이 식이섬유를 첨가한 가공식품을 섭취할 경우 배변량의 조절, 변비 및 비만조절, 혈청 지질의 감소와 같은 유용한 생리적 효과가 있을 것으로 기대된다. The effects of dietary fiber isolated from ascidian (Halocynthia roretzi) tunic on the changes of weight, total gut transit time, serum cholesterol and glucose level were investigated in rats. Twenty four male rats were divided into 4 groups and were fed a control diet and three fiber supplemented diets with 5, 10 and 20% of ascidian insoluble cellulose for 4 weeks, respectively. Food intake was not affected by the supplemented diet of ascidian cellulose but the body weight gain and food efficiency ratio were reduced in proportion to a feeding amount of ascidian cellulose. The fecal output and fecal water content were increased, gut transit time was shortened, and length of gut was elongated in all dietary fiber groups. Serum cholesterol, LDL-cholesterol, neutral lipid, phospho-lipid and serum glucose concentrations were lowered and HDL-cholesterol was increased in rats fed the ascidian insoluble cellulose diet in proportion to a feeding amount of ascidian cellulose.
육홍선(Hong-Sun Yook),차보숙(Bo-Sook Cha),김동호(Dong-Ho Kim),이주운(Ju-Woon Lee),변명우(Myung-Woo Byun) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.7
감마선 조사(10 kGy)된 멸치액젓의 유전독성학적 안전성 시험을 수행하기 위해 Salmonella Typhimurium TA98, TA100, TA1535, TA1537과 E. coli WP2 uvrA 균주를 사용한 복귀돌연변이시험과 Escherichia coli PQ37을 이용한 SOS chromotest 및 CHL 세포를 이용한 염색체 이상시험을 활성 대사효소계 미적용 및 적용하에 실시하였고, ICR 마우스의 골수세포를 이용한 in vivo 소핵세포실험을 수행하였다. 감마선 조사(10 kGy)된 멸치액젓은 위의 3가지 in vitro 실험에서 비조사된 멸치액젓과 마찬가지로 음성으로 나타났다. 또, 감마선 조사 및 비조사된 멸치액젓의 in vivo 소핵세포실험에서도 소핵이 발견되지 않았다. 따라서 10 kGy까지 감마선 조사된 멸치액젓은 위 수행된 in vitro 및 in vivo 유전독성시험을 실시한 결과, 음성을 나타낸 것으로 보아 유전독성학적으로 돌연변이원성이 없는 것으로 확인되었다. Gamma irradiations at 5 or 10 kGy were applied to salted and fermented anchovy sauce, for improving the hygiene quality and evaluating the genotoxicological safety. In vitro genotoxicological safety of irradiated sauces was evaluated by Salmonella Typhimurium (TA98, TA100, TA1535 and TA1537) and E. coli WP2 uvrA reversion assay, SOS chromotest (Escherichia coli PQ37), and chromosome aberration test (Chinese hamster lung fibroblast cells) in the absence or presence of an exogenous metabolizing system (S9 mix). The gamma-irradiated samples were not significantly different from nonirradiated-control for three in vitro tests (p<0.05). In vivo micronucleus test using ICR mice (male) was not significantly different from the control at p<0.05. The salted and fermented anchovy sauce exposed to 5 or 10 kGy-gamma ray revealed negative results in these three in vitro mutagenetic tests and in vivo micronucleus test upto 50,000 μg/plate, respectively. The results indicated that 5 or 10 kGy gamma-irradiated salted and fermented anchovy sauces did not show any mutagenicity.