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버섯 세균성갈색무늬병원균(Pseudomonas tolaasii)의 독소 (tolaasin) 저해균 Pseudomonas sp. HC1의 대량배양을 위한 최적 배양조건
이찬중,유영미,한주연,전창성,정종천,문지원,공원식,서장선,한혜수,차재순 한국버섯학회 2014 한국버섯학회지 Vol.12 No.1
Several bacteria are known as the causal agents of diseases of the cultivated button mushroom(Agaricus bisporus) and oyster mushroom(Pleurotus ostreatus). Pseudomonas tolaasii is the causal agent of brown blotch disease of commercial mushrooms. Pseudomonas sp. HC1 is a potent biological control agent to control brown blotch disease caused by Pseudomonas tolaasii. This can markedly reduce the level of extracellular toxins (i.e., tolaasins) produced by Pseudomonas tolaasii, the most destructive pathogen of cultivated mushrooms. To define the optimum conditions for the mass production of the Pseudomonas sp. HC1, we have investigated optimum culture conditions and effects of various nutrient source on the bacterial growth. The optimum initial pH and temperature were determined as pH 5.0 and 20oC, respectively. The optimal culture medium for the growth of tolaasin inhibitor bacterium was determined as follows: 0.9% dextrin, 1.5% yest extract, 0.5% (NH4)2HPO4, 4mM FeCl3, and 3.0% cysteine.
팽이버섯 재배에서 미강 대체용 보리가루의 적정 첨가 비율
이찬중,문지원,유영미,한주연,정종천,공원식,이병의 한국버섯학회 2015 한국버섯학회지 Vol.13 No.3
This study was carried out to investigated optimum additive ratio of barley flour for substitution of rice bran at cultivation of winter mushrooms. Mycerial growth was faster according to increase of barley flour ratio than those of controls, but only some slow at the addition of 10% barley flour. Yields of fruiting body show the highest to 165.4 g/850 ml of medium which are the addition 30% of barley flour and not significant difference of yields up to the addition of 70% barley flour. Diameter of pileus was the highest at the addition of 30% barley flour. Hardness of pileus and stipes were the highest at the addition of 10% barley flour. The L value of stipes were the hight at the addition of barley flour, but the L value of pileus were decreased at the addition of barley flour, but there was no significant difference in the a-value and the b-value.
이찬중,이정훈,민경진,박혜성,이은지 한국버섯학회 2020 한국버섯학회지 Vol.18 No.2
This study sought to determine the optimal mixing ratio of Korean cultivated Artemisia annua for production of functional oyster mushrooms. After 23 days of cultivation, mycelial growth was 12.7 cm in medium supplemented with 5% A. annua and 12.5 cm in control. Mycelial growth progressively slowed with the addition of A. annua, with barely any growth (2.1 cm) in the presence of 70% A. annua. Mycelial density was high density without significant difference between treatments. The pileus diameter was greater in the presence of A. annua than in the control, but the pileus thickness was only slightly higher compared to the control. The stipes thickness was greatest for 15% A. annua, and the length of stipes was longest at 10% A. annua, but was lower than the value of the control. The L value of the fruiting zone was highest 10% firewood, and the L value of freshwater was highest at 5% A. annua. The fruiting body yield was highest to (122 g/850 ml) in medium supplemented with 5% A. annua but markedly decreased at higher levels of A. annua.
Effects of Solar Heating for Control of Pink Root and Other Soil-borne Diseasesof Onions
이찬중,이종태,문진성,하인정,김희대,김원일,천미건 한국식물병리학회 2007 Plant Pathology Journal Vol.23 No.4
These experiments were carried out to examine efficacy of soil solarization for control of pink root disease by means of mulching with transparent polyethylene sheets in the hot season. The effects of soil solarization on incidence of pink-root disease caused by Pyrenochaeta terrestris and on onion growth and on populations of soil fungi were investigated. Solarization was dramatically effective in reducing pink root incidence in onion seedling and harvested onion bulb. A 30-day and 40-day solarization treatment significantly improved seedling survival and increased yield of ‘Changnyeongdeago’ onion while decreasing incidence of pink root. Populations of soil fungi from fields planted to onion were assayed on selective media. Solarization treatment was effective in reducing populations of P. terrestris, Pythium spp., and Rhizoctonia sp. in soil. Increase of yield of onion bulbs was associated with control of soilborne pathogenic fungi. Soil solarization had beneficial effects on yield, bulb diameter, or incidence of pink root.
가래나무(Juglans mandshurica) 톱밥의 첨가량이 느타리버섯의 수량에 미치는 영향
이찬중,정종천,전창성,문지원,공원식,서장선,박기춘,신유수,이정훈 한국버섯학회 2013 한국버섯학회지 Vol.11 No.4
This study was carried out to investigated optimum mixing ratio of Korean natural Juglans mandshurica using as functional plants. Total nitrogen and carbon source of J. mandshurica was 0.21% and 46.0%, respectively and C/N ratio was 219. Total nitrogen source and pH of substrate mixed with J. mandshurica was 2.3~2.9 and 5.0, respectively. The contents of CaO, MgO and Na2O at J. mandshurica media were higher at harvest media than inoculation media. Mycelial growth was the fastest at J. mandshurica 20%, and slower by increase of J. mandshurica substrate. Yields of fruiting body show the highest to 163 g/850 mL of medium which are addition 10% of J. mandshurica sawdust, and diameter and thick of pileus were the highest, too. The L value of pileus and stipes were increased by increase of J. mandshurica substrate, but there was no significant difference in the a-value and the b-value. The contents of P2O5 and K2O of fruiting body were increased at J. mandshurica substrate, but there was no significant difference in contents of CaO, MgO and Na2O. The contents of Cu of fruiting body was decreased by increase of J. mandshurica substrate, but Fe, Mn and Zn were increased.
양송이 배지교반기 시제품을 활용한 배지 조제시 야외 발효단계별 배지의 특성
이찬중,유병기,이은지,박혜성,공원식,김영호 한국버섯학회 2018 한국버섯학회지 Vol.16 No.1
This study was performed to compare medium characteristics during the composting stage for medium turning performed using an excavator agitator and a prototype medium turner in button mushroom cultivation. The changes in temperature in the medium did not significantly differ between the treatments until the 3rd turn performed using the excavator agitator. However, during the 4th and 5th turns, the temperature increased during turning with the prototype medium turner. During outdoor composting, various types of microorganisms such as thermophilic bacteria (Bacillus spp.), Actinomycetes, fluorescent Pseudomonas spp., and filamentous fungi were found to be distributed in the medium. The counts of aerobic bacteria and fluorescent Pseudomonas spp. did not significantly differ between treatments, and the counts of thermophilic bacteria and thermophilic actinomycetes were slightly higher during turning with the prototype medium turner. The rice straw was slightly shorter and water content lower for the prototype medium turner. There was no significant difference between pH and EC treatments. The L, a, and b values tended to increase on turning with the prototype medium turner.
양송이 재배에서 볏짚 배지의 발효 단계별 관여 미생물의 분포양상 및 특성
이찬중,유영미,문지원,정종천,공원식,김용균,이병의,윤민호,사동민,Lee, Chan-Jung,Yoo, Young-Mi,Moon, Ji-Won,Cheong, Jong-Chun,Kong, Won-Sik,Kim, Yong-Gyun,Lee, Byung-Eui,Yoon, Min-Ho,Sa, Tong-min 한국균학회 2017 韓國菌學會誌 Vol.45 No.1
In this study, we analyzed the densities and taxonomic characteristics of various microorganisms that play important roles in Agaricus bisporus culture medium composting, and examined changes in the levels of decomposition-related enzymes secreted by these microorganisms. Various microorganisms such as thermophilic bacteria, actinomycetes, fluorescent Pseudomonas spp., and filamentous bacteria are closely associated with culture medium composts of Agaricus bisporus. The population densities of microorganisms change, and harmful bacteria disappear during thermophilic composting. Psychrobacter sp., Pseudomonas sp., Bacillus sp., and Pseudoxanthomonas sp. accounted for the highest proportion of bacteria in the culture media during outdoor composting, whereas Bacillus sp. and Psychrobacillus sp. were dominant after pasteurization. Cellulose and hemicellulose enzymes of the microorganisms were important at an early stage of rice straw composting and after decomposition of carbon sources, respectively. Microorganisms that secreted these enzymes were present in the second and third turning stage of composting. 본 연구는 양송이 배지의 관행적인 발효기술 개선에 대한 기초자료를 제공하기 위하여 배지 발효에 중요한 역할을 하는 다양한 미생물에 대한 밀도변화 및 분류학적인 특징을 밝혔고, 이들 미생물이 분비하는 세포벽 분해 관련 효소활성 변화를 조사하였다. 양송이 배지 발효와 밀접한 관련이 있는 미생물의 밀도 변화를 분석한 결과 고온성 세균, 방선균, 형광성 Pseudomonas spp., 사상균 등 다양한 미생물들이 분포하였으며, 고온성 발효가 진행됨에 유해균은 사멸되는 경향을 보였다. 야외발효 과정에서는 Psychrobacter속, Pseudomonas속, Bacillus속, Pseudoxanthomonas속이 가장 많이 분포하였고, 후발효 완료 배지에서는 Bacillus속, Psychrobacillus속이 우점하였다. 이러한 볏짚 발효 과정 중 분리한 미생물의 효소활성은 발효 초기에 cellulose 분해효소가 먼저 배지에 작용하여 탄소원을 분해한 후 hemicellulose 분해효소가 부차적으로 작용하는 양상을 보였고, 이들 효소를 분비하는 미생물들은 2차와 3차 뒤집기에서 많이 분포하였다.