겔형태 인공누액이 각막실질세포 및 결막상피세포에 미치는 영향

이종수,김은희,김수진,이지은,김나미,Jong Soo Lee,Eun Hee Kim,Soo Jin Kim,Ji Eun Lee,Na Mi Kim 대한안과학회 2010 대한안과학회지 Vol.51 No.12

Purpose: To evaluate the biological effects and cytotoxicity of gel-type artificial tears on human corneal keratocytes and conjunctival cells in vitro. Methods: Human corneal keratocytes and conjunctival epithelial cells were exposed to Soothe® and Systane® at variable concentrations. Evaluations were conducted through an MTT-based calorimetric assay to measure the metabolic activity and through a lactate dehydrogenase (LDH) assay to assess cellular damage. Apoptotic response was examined using fluorescent microscopy and flow cytometric analysis, and cellular morphologic results were evaluated with a transmission electron microscope. Results: The inhibitory effects of corneal keratocyte and conjunctival cell proliferations increased at higher concentrations and longer exposure times to Soothe® and Systane®. The LDH titers increased after Soothe® exposure, but showed no significant difference after Systane® exposure. Soothe® and Systane® treatments both produced fluorescence, representing apoptotic cells. In flow cytometry, the maximal apoptotic response was observed for both types of artificial tears, although Systane® showed less edema, as well as reduced cytoplasmic and nuclear cell degeneration compared to those of Soothe®. Conclusions: The apoptotic responses of Soothe® and Systane® are associated with inhibitory effects of human corneal keratocyte and conjunctival epithelial cell proliferations. To inhibit the cellular proliferation of human corneal keratocytes and conjunctival epithelial cells, Systane® may be less severe than Soothe® at higher concentrations and longer exposure times. J Korean Ophthalmol Soc 2010;51(12):1643-1651

Candida sp. L-16이 생성하는 Xylitol dehydrogenase의 생성조건에 관한 연구

이종수 대구산업정보대학 1995 논문집 Vol.9 No.2

The opitmal cultural conditions such as carbon and nitrogen sources, cultural temperature, initial pH, aeration and cultural time for production xylitol dehydrogenase which catalyzed the conversion of xylitol to xylulose from Candida sp. L-16. were investigated. The maximum yield of xylitol dehydrogenase was observed with cultivation in a medium containing 5.0% D-xylose as a carbon source and 0.3% yeast extract, 0.3% malt extract and 0.5% bactopeptone as nitrogen sources and pH 4.5. The optimal conditions for production of xylitol dehydrogenase were 28℃ of cultural temperature and 4.5 of initial pH. It was found that the production of enzyme reached a maximum in a 3 days of cultivation in the 100㎖Erlenmeyer flask containing 40㎖ broth.

몇종의 세균과 Saccharomyces cerevisiae에 대한 식품첨가물의 향균 특성

이종수,오준세,김나미,금종화,이석건,Lee, Jong-Soo,Oh, Jun-Sei,Kim, Na-Mi,Keum, Jong-Hwa,Lee, Suk-Kun 배재대학교 자연과학연구소 1996 自然科學論文集 Vol.8 No.2

현재 식품첨가물로 많이 사용되고 있는 유기산과 안정제 및 색소 등의 각종 세균과 Sacch. cerevisiae 에 대한 향균 특성을 조사하였다. 산도 조절용으로 사용되고 있는 젖산, 사과산, 호박산 및 주석산은 L. acidophilus와 Sacch. cerevisiae에 대하여 향균성이 없었으나 B. subtilis등의 세균에 대하여는 향균성이 있었고 특히 사과산은 P. aeruginosa에 대하여 강한 향균력이 있었다.(최소생육저지농도 : 0.05%). 안정제로서의 알긴산과 펙틴은 B. subtilis, E. coli, P. aeruginosa에 대하여 비교적 강한 향균성을 보였고 L. acidophilus에 대하여는 향균성이 없었다. 황색 색소(홍화엘로우)와 적색 색소(Red powder-N)는 향균성이 없었고 표백제인 $NaHSO_3$의 세균에 대한 최소생육저지농도는 0.05%, Sacch. cerevisiae에 대하여는 0.5%로 향균성이 있었다. In order to survey the safety of some food additives, antimicrobial activity of acidulants, stabilizers, antioxidants, natural coloring materials and bleaching agents against 5 strains of bacteria and Sacch. cerevisiae were investigated by dilution method and minimal inhibitory concentration(MIC) method. Malic acid as acidulants displayed the effective antimicrobial activity in vitro against P. aeruginosa and its MIC is 0.05%. Alginic acid and pectin as stabilizer also displayed strong antimicrobial activity against B. subtilis, E. coli and P. aeruginosa, and tannin(antioxidants) and $NaHSO_3$ displayed antimicrobial activity against all bacteria tested. However gums(Arabia, Xanthan, Gua) and natural coloring materials(Hongwha Yellow, Red powder-N) were not affected to growth of bacteria and Sacch. cerevisiae.

공무원의 전문성 향상방안으로서 경력개발제도(CDP)에 관한 연구 : CDP의 시각에서 분석한 한국 인사행정체계의 문제점과 개선방안

이종수 한국행정연구원 2002 韓國行政硏究 Vol.11 No.4

인사행정의 본질적 과제 가운데 조직의 수요와 개인의 욕구를 결합시키는 역할이 중요성을 더해가고 있다. 이를 위해 최근 각 국 정부는 경력개발제도(CDP)를 주목하고 있으며, 한국의 중앙인사위원회도 2002년 대통령에게 경력개발제도의 도입검토를 보고한 바 있다. 본 연구에서는 최근 정부의 인력관리에 적용가능성이 타진되고 있는 경력개발제도의 개념을 소개하고, 공무원들의 이에 관련된 인식을 분석하며, 나아가 경력개발제도의 적용을 위한 개선과제를 검토하여 보았다. 이를 위해 연구방법으로 문헌연구와 설문조사, 심층면접, 브레인스토밍(Brain Storming)방법을 병행하였다. 분석 결과, 한국의 공무원 인사행정 체계에서 나타나는 계급제적 채용제도, 직위분류제도의 미정착이 경력개발제도의 요소를 필요로 하는 기본배경이 되고있다. 조직문화와 순환보직제도, 짧은 보직기간, 직무수행을 위한 know-how의 취득원과 교육훈련의 불일치 등은 경력개발을 통한 전문성 제고를 저해하는 요소로 분석되었다. 결론적으로, 경력개발제도는 한국 공무원의 전문성 제고와 직무수행 역량의 향상을 위한 다양한 쟁점을 제기하고 있고, 발전방안을 시사하고 있다. The findings suggest a modification to the current uni-dimensional approach to study organizational culture. Public personnel administration has been subjected to virtually uninterrupted wave of reforms under the legacy of New Public Management. It has been the key area of governmental reforms during the past two decades. Many efforts were made to initiate structural changes also in Korea. However. since the feature of traditional class system prevails in the human resource management of the Korean government, it is difficult to achieve the utmost cfficiency. This paper examines major issues of public personnel administration in Korea, particularly form the viewpoint of CDRCareer Development Program). The purpose of this paper is threefold. First, the notion of CDP is introduced to the field of public personnel administration research. Second, the current problems of personnel administration in Korea are analysed from the views of Career Development Program. Third, policy alternatives are developed for the improvement of job competency of public officials in Korea. To accomplish these objectives, this research adopts questionnaire survey method and Brain Storming approach in part. Seven policy alternatives are presented in this study. In order to successfully achieve the unification of South and North Korea, it is necessary to carry out a systematic study as to how the political and administrative systems of North Korea could be transformed into a democratic system. Such a transformation implies the fundamental reconstruction of a nation, accompanied by drastic changes in political and administrative systems. In the case of the former East Germany, the nations statehood itself changed as a result of German unification. Regardless of the method, if the product of Korean unification were a democratic, constitutional, free-market economy and a welfare society, North Korea would inevitably undergo a transformation of its state system. In particular, the transformation of human

전침이 중풍재활에 미치는 영향에 대한 문헌적 고찰

이종수,심우진,Lee, Jong-Soo,Sim, Woo-Jin 척추신경추나의학회 2002 대한추나의학회지 Vol.3 No.1

Objectives : Electroacupuncture(EA) has been suggested as a treatment for stroke rehabilitation. But whether, how much, by what mechanism and when it is effective has not been answered satisfactorily. Therefore it is important to critically review clinical trials and laboratory researches about EA for stroke rehabilitation. Subjectives : We researched various recent sources of EA for stroke rehabilitation such as medical journals and especially tried to review methodologically best randomized controlled trials(RCTs). Results and Conclusions : 1) EA increases brain plasticity, activity, blood flow and secretion of neuropeptides in CNS. 2) EA is significantly effective at the case that more than half of the neural motor pathway is reserved. 3) The acupoints, frequncy and intensity of EA should be determined by patient-specific symptoms of stroke. 4) More studies is needed for merdian functions for stroke rehabilitation.

피기백 인공수정체를 이용한 백내장 수술 후의 잔존 굴절 이상의 교정 1예

이종수,김영희,박정열,김성일,Jong Soo Lee,MD,PhD,Young Hee Kim,MD,Jung Yul Park,MD,Sung Il Kim,MD 대한안과학회 2014 대한안과학회지 Vol.55 No.12

Purpose: To report a case of corrected residual refractive error after cataract surgery with sulcoflex piggyback intraouclar lens (IOL). Case summary: A 77-year-old man was diagnosed as having hypermature cataract in the right eye and his corrected visual acuity in the same eye was hand-motion before surgery. Refractive error was +9.0 diopter (D) 6 months after conventional cataract surgery in the right eye. The authors performed additional cataract surgery using a piggyback method inserting a +10.0 D IOL in the ciliary sulcus. Four weeks after surgery, his refractive error was -1.25 D, visual acuity increased to 120/200 and 60/200 (with and without correction, respectively). No complication was observed during follow-up time and the patient was satisfied with his results. Conclusions: Correction of residual refractive error after cataract surgery with sulcoflex piggyback IOL is safe and effective. J Korean Ophthalmol Soc 2014;55(12):1890-1894

정상 및 원추각막에서 각막 전, 후면의 융기 및 시상면만곡도의 비교분석

이종수,이승욱,이승민,이미현,Jong Soo Lee,Seung Wook Lee,Seung Min Lee,Mi Hyun Lee 대한안과학회 2012 대한안과학회지 Vol.53 No.10

Purpose: To compare changes of anterior and posterior corneal elevation, and sagittal curvature according to the severity of keratoconus and to compare differences between keratoconus and normal cornea. Methods: A total of 81 eyes diagnosed with keratoconus and 20 eyes of normal subjects were evaluated with a Pentacam Scheimpflug camera. The keratoconus eyes were divided into 3 groups according to mean keratometer (K): mild (K ≤ 47.0 diopters (D)), moderate (47.0 to 52.0 D), and severe (52.0 D≥). The following parameters were obtained to evaluate the correlation of keratoconus: corneal thickness, anterior and posterior corneal elevation, and sagittal curvature. Results: Out of 81 keratoconus eyes, 56 eyes were mild, 12 eyes were moderate, and 13 eyes were severe keratoconus. The mean central corneal keratometer, anterior and posterior corneal elevation, and sagittal curvature of the keratoconus eyes were 49.7 D, 22.07 μm, 38.16 μm, 52.76 D and the values increased statistically compared to the normal eyes. Furthermore, the values increased significantly with the severity of keratoconus. ROC curve analysis showed the estimated meaningful value for anterior and posterior corneal elevation and sagittal curvature of keratoconus for diagnosis; there were no diagnostic values for corneal thickness and refractive power. Conclusions: The index of 5.5 μm for mean anterior elevation, 12.5 μm for mean posterior elevation, and 44.5 μm for mean sagittal curvature using the PentacamⓇ are useful to diagnose keratoconus. Variation of anterior and posterior elevation, and sagittal curvature measured by PentacamⓇ are useful in understanding the process of keratoconus. J Korean Ophthalmol Soc 2012;53(10):1432-1437

미생물 일반 , 생리 및 대사 재래식 메주에서 분리한 효모들의 각종 효소활성과 가능성

이종수,이성훈,권수진,안철,유진영 ( Jong Soo Lee,Sung Hun Yi,Su Jin Kwon,Cheol Ahn,Jin Young Yoo ) 한국미생물생명공학회 1997 한국미생물·생명공학회지 Vol.25 No.5

미생물 일반 , 생리 및 대사 재래식 메주로부터 효모의 분리 , 동정 및 배양조건

이종수,이성훈,권수진,안철,유진영 ( Jong Soo Lee,Sung Hun Yi,Su Jin Kwon,Cheol Ahn,Jin Young Yoo ) 한국미생물생명공학회 1997 한국미생물·생명공학회지 Vol.25 No.5

화침을 이용한 흉요추부 압박골절 치험 1례

이종수,한순현,Lee, Jong-Soo,Han, Soon-Hyun 척추신경추나의학회 2002 대한추나의학회지 Vol.3 No.1

Stable compression fractures are most often caused by trauma as traffic accidents. Stable compression fractures can refer pain to the mid-back, which is characterized by the pain in the thoracolumbar spinous prosess. recently we experienced a 19 years old man who had lumbago originating from Stable compression fractures and condition was improved through burning acupunture. 흉요추 이행부의 안정성 압박골절은 통증의 소실과 후만각 증가 억제에 의한 척추의 안정화이다. 이의 원인을 후방인대의 손상에 의한 손상으로 보고 프로로테라피(Prolotherapy)로 이의 치료를 시행 할 수 있다고 생각되며 한방적인 방법으로 화침을 적용할 수 있었다. 최근 저자는 흉요추 이행부의 안정성 압박골절에 의한 요배통 환자를 발견하고 화침치료를 통해 증세 회복에 도움이 되었기에 치료내용과 경과를 문헌고찰과 함께 보고하는 바이다.