확인 실험 수업에서 나타나는 초등교사들의 교수 행동 절차 분석

양일호 ( Il Ho Yang ),조현준 ( Hyun Jun Cho ),윤영란 ( Yeoung Lan Yoon ) 한국초등과학교육학회 2007 초등과학교육 Vol.26 No.4

Target DNA 염기서열 내에 존재하는 비상동성 간격이 상동성재조합을 이용한 클로닝 빈도에 미치는 영향

김재우,도은주,윤세련,정윤희,윤영호,임선희,선우양일,박인호,Kim Jae-Woo,Do Eun-Ju,Yoon Se-Lyun,Jeong Yun-Hee,Yoon Young-Ho,Leem Sun-Hee,Sunwoo Yangil,Park In-Ho 한국미생물학회 2005 미생물학회지 Vol.41 No.4

Transformation-associated recombination (TAR) 클로닝 법은 복잡한 게놈으로부터 염색체 내의 특정부위나 유전자를 선택적으로 분리할 수 있다. 이 방법은 목적 유전자에 근접한 작은 게놈DNA 염기서열 정보를 필요로 한다. 이 기술은 효모의 spheroplast transformation을 시키는 동안 목적으로 하는 유전자의 5' 또는 3' 서열을 포함하고 있는 TAR vector와 게놈DNA사이에서 일어나는 상동성재조합에 의해 이루어진다. 본 연구에서는 plasmid 모델시스템을 이용하여 target hooks 내에 존재하는 비상동성 염기서 열이 상동성재조합에 미치는 영향을 조사하였다. plasmid에 존재하는HIS3유전자와 변형시킨 his3-TRP1-his3 단편 사이의 상동성재조합의 효율은 $Ura^+$ 형질전환체의 형질분석에 의해 이루어졌다. $Ura^+$ 형질전환체의 수는 7종류의 서로 달리 변형된 his3-TRP1-his3 단편들을 사용하였을 매 거의 동일하게 나타났다. 그러나 $Trp^+His^+$ positive recombinants의 빈도는 변형된 his3-TRP1-his3 단편 내에 비상동성 영역에 부정확한 간격을 지닐 때 현저한 감소를 나타내었다. 이러한 결과로서, 부정확한 간격이 target hook과 substrate DNA 사이에 일어나는 상동성재조합을 방해하는 것으로 사료된다. 그러므로 이종간의 상동유전자를 클로닝 할 때에는 target hook내의 비상동성 염기서열이 존재한다면 이것이 정확한 간격을 지니는지 여부를 중요란 요인으로 고려해야 한다. Transformation-Associated Recombination (TAR) cloning technique allows selective isolation of chromosomal regions and genes from complex genomes. The procedure requires knowledge of relatively small genomic sequences that reside adjacent to the chromosomal region of interest. This technique involves homologous recombination during yeast spheroplast transformation between genomic DNA and a TAR vector that has 5' and 3' gene targeting sequences. In this study, we examined the effect of non-homologous spacing sequence in target hooks on homologous recombination using a plasmid model system. The efficiency of homologous recombination between the modified his3-TRP1-his3 fragments and HlS3 gene on plasmid were analyzed by the characterization of $Ura^+$ transformants. The numbers of $Ura^+$ transformant showed same level when seven different modified his3-TRP1-his3 fragments were used. But the percentage of positive recombinants. $Trp^+His^-$, dramatically decreased when used the modified his3-TRP1-his3 fragments contained incorrect spacing of nonhomologous region. As a result, we suggest that incorrect spacing inhibits the homologous recombination between target hook and substrate DNA. Therefore, we should consider the correct spacing in target hook when the target hook are used for cloning of orthologue gene.

팽대부 종양에서의 경십이지장 유두부 절제술

김준우(Jun Woo Kim),황윤진(Yoon Jin Hwang),김양일(Yang Il Kim),윤영국(Young Kook Yun) 대한외과학회 2001 Annals of Surgical Treatment and Research Vol.60 No.4

The Effect of Acupuncture on the Intestinal Smooth Muscle Dysfunction Caused by Enteric Bacterial Infection in Weaning Piglet

김태완,나준호,성태식,강정우,남치주,최민철,윤여성,양일석,Kim, Tae-wan,La, Jun-ho,Sung, Tae-sik,Kang, Jeong-woo,Nam, Tchi-chou,Choi, Min-cheol,Yoon, Yeo-sung,Yang, Il-suk The Korean Society of Veterinary Science 2003 大韓獸醫學會誌 Vol.43 No.3

It is now generally accepted that acupuncture is effective in diarrhea caused by bacterial infection. However, its effect on the intestinal smooth muscle dysfunction is not clear. Therefore, we investigated the effect of acupuncture therapy at Jiao-chao (GV-1) on the intestinal muscle dysfunction in weaning piglets orally infected by Escherichia coli. The animals are divided into four groups; 1) E. coli + no-treatment, 2) E. coli + antibiotic, 3) E. coli + acupuncture, 4) normal group. In the three E. coli infected groups, low frequency electrical field stimulation (EFS, 1 Hz) provoked triphasic responses composed of initial relaxation followed by on-contraction and off-contraction. While in the normal group, EFS (1Hz) induced biphasic responses composed of relaxation during the stimulation and off-contraction. At the high frequency (16Hz) EFS, both on-contraction and off-contraction of the E. coli + antibiotic, E. coli + acupuncture and the normal group were larger than those of the E. coli + no-treatment group. In the non-adrenergic non-cholinergic (NANC) condition, only biphasic responses occurred to EFS in all experimental groups and the off-contraction of E. coli + antibiotic, E. coli + acupuncture and the normal group were larger than those of the E. coli + no-treatment group. The response to carbachol of those three groups was also significantly greater than that of the E. coli + no-treatment group. These results suggest that acupuncture is as effective as antibiotic in the dysfunction of colonic circular muscle caused by E. coli infection. The maintenance of contractile neuromuscular transmission seems to be involved in the mechanism of the acupuncture effects on diarrhea.

간이식 및 절제술에서 술전 간문맥 폐색이 있던 증례에 대한 고찰

김준우(Jun Woo Kim),황윤진(Yoon Jin Hwang),윤영국(Young Kook Yun),김양일(Yang Il Kim) 한국간담췌외과학회 1999 한국간담췌외과학회지 Vol.3 No.1

A Study on the Development of Wind Turbine using the lift and drag for the Offshore

김남훈(Kim, Namhun),이병성(Lee, Byeongseong),윤양일(Yoon, Yangil),오진석(Oh, Jinseok) 한국신재생에너지학회 2010 한국신재생에너지학회 학술대회논문집 Vol.2010 No.06

This is the research of wind turbine that is designed to supply power to offshore buoy system. In order to reach maximum efficiency in limited space, vertical axis wind turbine was used. Vertical axis wind turbine system that was applied in this research has the form of lift and drag blade combined to achieve high efficiency at both high and low speed. In addition, support system was designed and developed to suit the offshore condition.

날개 길이 및 후면부 절개 비율에 따른 해상용 수직축 풍력발전기 특성 평가

김남훈(Kim, Namhun),김경수(Kim, Kyenogsoo),윤양일(Yoon, Yangil),오진석(Oh, Jinseok) 한국신재생에너지학회 2011 한국신재생에너지학회 학술대회논문집 Vol.2011 No.05

해상용(offshore) 부이(bouy)는 선박의 항로를 지시하거나 암초, 침몰선 등 항해상의 위험물을 알리기 위해 사용 되며, 야간을 위해 등화장치를 설치한 것을 등부표라 한다. 등부표는 야간 점등을 위해 자체 전력 생산시스템을 갖추고 있으나, 기존의 태양광을 이용한 전력 시스템은 해상 환경에 따른 제약이 많아 안정적인 운영이 어려우므로 풍력 발전기(wind turbine)를 이용한 하이브리드 전력 생산시스템으로의 전환이 필요한 실정이다. 선행 연구는 수직축(vertical axis) 양력(lift) 및 항력(drag) 조합형 해상용 풍력발전기 개발에 대하여 수행하였으나, 본 논문에서는 풍력발전기의 효율 증대를 위해 날개 길이 및 후면부 절개 비율에 따른 수직축 풍력발전기 특성에 대하여 연구하였다. 풍력발전기의 설치조건은 선행연구와 동일하게 등명구 교체 작업을 원활하게 하기 위하여 설치 공간을 1m{times}1m로 제한하였으며, 등부표의 구조를 고려하여 최상단에 지지 프레임을 별도로 구성 하였다. 풍력발전기의 블레이드는 0.6mm의 알루미늄 박판을 절곡하여 NACA 4418의 외형을 가지도록 제작하였고, 블레이드 설계 시 에어포일의 후면부를 절개하여 양력과 항력을 효과적으로 이용하며 저속과 고속에서 높은 효율을 가지도록 설계하였다. 또한 블레이드 날개 길이와 후면부 절개 비율에 따른 풍력발전기 특성을 실험을 통해 비교하여 기준 해상 풍속에서 블레이드 설계 최적화를 수행하였으며 비교 모델 대비 약32% 발전량이 증가한 설계변수 조합을 구하였다.

4D-PTV에 의한 수평자유축 조류발전시스템 유동특성 분석

도덕희(Deog Hee Doh),조효제(Hyo Jae Jo),최진형(Jin Hyung Choi),이재민(Jae Min Lee),이연원(Yeon Won Lee),윤양일(Yang IL Yoon),스즈키키요미(Kiyomi Suzuki) 한국유체기계학회 2009 유체기계 연구개발 발표회 논문집 Vol.2009 No.-

Flow characteristics of a fish-type current power generation system have been investigated by the use of a volumetric PTV. Three types of the turbine blades have been tested under the current speed l.35knot, 1.5knot and 2knot. The width of the blade installed around the body is 5㎜. The load test has been carried out under the same flow conditions. All flow features have been investigated when the blade have come to the same rotating angle position using a position sensor. Among the models it has been verified that the S65 showed the highest voltage generations. For the case of S65 turbine model, the deficit of the velocity magnitude in the wake region seems to contribute the transfer of moments from the current fluids to the turbine blade.

병원, 학교, 사업체급식소 영양사의 직무만족, 직무특성 및 조직몰입성 상관관계 분석

양일선,이진미,차진아,윤정신 대한영양사협회 1997 대한영양사협회 학술지 Vol.3 No.1

Purposes of this study were to: (a) measure the levels of job satisfaction, (b) investigate the degree of job characteristics, (c) determine the levels of organizational commitment, (d) investigate relationships between demographic variables and job satisfaction, and (e) examine the relationship between organizational commitment, job characteristics and job satisfaction. Questionnaire was developed and mailed to 300 dietitians who are currently working in school, hospital and industry foodservices. A total of 177 questionnaires were usuable: resulting in a 59% response rate. Results of this study were summarized as follows: 1. Approximately half of the respondents(46.9%) indicated that they were 26 to 30 years old. A total of 112 respondents(63.3%) were unmarried. A bachelor's degree was reported as the most common education level(46.9%) while 6.2% of the participants reported a master's degree. Years of employment in professional practices were ranged from 2 to 5. Approximately 40% of the respondents had earned over \900.000 per month. 2. Work itself facet of the JDI was with the highest job satisfaction mean score followed by supervision, co-workers, wage and promotion. 3. In total job satisfaction score, dietitians in school foodservices were a significant lower than dietitians in industry foodservices and hospital foodservices. 4. A significant relationship was found for demographic factors and four job facets in terms of job itself, co-workers, wage, and promotion. 5. A work itself was the most important facet to the dietitian followed by wage and promotion. 6. Task identity of the JCI facets was the most prevalent job characteristics followed by variety, dealing with others, feedback and friendship. 7. Three groups of dietitians had no significant difference in total job characteristics score. 8. A group of dietitians with a higher job satisfaction score had a significant higher score in job variety and job autonomy. 9. Organizational commitment mean score with dietitians was 1.88. 10. Job satisfaction score for five facets and total job satisfaction were significantly correlated with organizational commitment score. 11. Job charateristics with job variety, job autonomy, feedback, task identity, friendship were positively correlated with organizational commitment.

출아효모에서 TAR 클론닝법을 이용한 고등동물의 게놈으로부터 특정 염색체 부위의 분리

박정은,윤영호,이윤주,김광섭,윤세련,안태진,임선희,선우양일 동아대학교 기초과학연구소 2003 基礎科學硏究論文集 Vol.20 No.1

복잡한 게놈 분석에 용이하도록 효모의 인공 염색체(YAC) 클론닝 시스템은 발달되어왔다. YAC은 박테리아의 인공 염색체(BAC)보다 더 큰 단편을 클론닝할 수 있고, 또한 클론된 단편을 쉽게 변형시킬 수 있다. 형질전환과 연계된 재조합법(Transformation-Associated Recombination; TAR)은 게놈 라이브러리를 만들지 않고 직접 게놈 DNA로부터 분리하고자 하는 유전자나 특정 염색체 부분을 클론닝 할 수 있는 방법이다. 이 방법은 spheroplast transformation을 수행하는 동안, 목적으로 하는 유전자의 5' 그리고 3' 염기 배열(hooks)을 지닌 TAR 벡터와 게놈 DNA 사이에서 일어나는 상동성 재조합에 의해 이루어진다. 효모 내의 in vivo 재조합을 이용한 TAR 클론닝법은 복잡한 게놈으로부터 목적의 염색체 부분을 원형 YAC의 형태로서 선택적으로 분리할 수 있다. 그러므로 TAR 클론닝 법은 특정 염색체로부터 YACs을 만드는데 매우 유용하여, 전체 게놈으로부터 특이적 유전자나 유전자의 family를 분리하는데 효과적인 방법으로 사료된다. Yeast artificial chromosome (YAC) cloning systems have advanced the analysis of complex genomes considerably. They permit the cloning of larger fragments than do bacterial artificial chromosome systems, and the cloned material is more easily modified. Transformation-associated recombination (TAR) is a cloning technique that allows specific chromosomal regions or genes to be isolated directly from genomic DNA without prior construction of a genomic library. This technique involves homologous recombination during spheroplast transformation between genomic DNA and a TAR vector that has 5' and 3' gene targeting sequences (hooks). Using in vivo recombination in yeast, TAR cloning selectively isolates, as circular YACs, desired chromosome segments or entire genes from complex genomes. We propose that TAR cloning can provide an efficient means for generating YACs from specific chromosomes and that TAR cloning may be useful for isolating families of genes and specific genes from total genome DNA.