존 콜린스의 관점으로 본 디아코노스 예수 -요한복음을 중심으로-

유용욱 ( Yong-ouk You ) 기독교학문연구회 2020 신앙과 학문 Vol.25 No.4

‘Diakonia’ has been used as a representation of Christian social welfare activities, church services and relief. The reason is that the origin of the word ‘diakonos’ means ‘a person who is waiting at the table’ traditionally. However John N. Collins interpreted the original meaning of diakonia as ‘go-between’ or ‘messenger’. In the Gospel of John, the use of diakonia to refer to Jesus' Ministry has never appeared, but the spirit of diakonia appeared in Jesus’ Ministry in the Gospel of John. Jesus has a self-consciousness that He is the Go-between in the Gospel of John. Another self-consciousness that Jesus has is the Messenger of His Father. Jesus has been the exclusive and chosen only Diakonos of God the Father, but His Ministry of Diakonia has been succeeded to his disciples and the church. The disciples and the church are commissioned by the Diakonos Jesus to perform the mission as the mediators between God and the world and as messengers of God on His behalf.

팬데믹과 기독교선교

유용욱 ( Yong-ouk You ) 기독교학문연구회 2021 신앙과 학문 Vol.26 No.1

The COVID19 pandemic facing the world in recent years is a widespread epidemic unprecedented in human history. Pandemics have long accompanied human history, threatening church services and Christian missions. However, the pandemic was not always negative for Christian missions, and sometimes gave Christianity a chance for mission. The purpose of this study is to examine how the pandemic has historically influenced Christian missionary work, and to gain insight to find the direction in which Christian mission should go in the current COVID19 pandemic situation. There were two major pandemics in the early Christian era. At that time, Christians of the early church saw the care of patients as a way to practice Christ's love even if they risked death. This true practice of love impressed the Romans and contributed greatly to Christian mission. In the era of religious reform, the reformers not only cared for patients, but also practiced the love of Christ by presenting the hope of eternal life after death. In the early days of Protestant missionary work in Korea, People with infectious diseases were cared for in Christian mission hospitals, which changed their perception of Christianity. These educational and medical ministries greatly contributed to the Korean Protestant mission.

석류피 에탄올 추출물의 항치아우식 활성

유용욱(Yong Ouk You),유현희(Hyeon Hee Yu) 한의병리학회 2007 동의생리병리학회지 Vol.21 No.1

히알루론산이 골 형성에 미치는 영향에 관한 실험적 연구

조용민(Yong-Min Cho),민승기(Seung-Ki Min),김수남(Soo-Nam Kim),유용욱(Yong-Ouk You) 대한구강악안면외과학회 2002 대한구강악안면외과학회지 Vol.28 No.3

Hyaluronic acid (HA) is an almost essential component of extracellular matrices. Early in embryogenesis mesenchymal cells migrate, proliferate and differentiate, in part, because of the influence of HA. Since the features of embryogenesis are revisited during wound repair, including bone fracture repair, this study was initiated to evaluate whether HA has an effect on calcification and bone formation in an in vitro system of osteogenesis. Mouse calvaria Pre-osteoblast (MC3T3-E1) cells were cultured in α-MEM medium with microorganism-derivative hyaluronic acid that was produced by Strep. zooepidemicus which of molecular weight was 3 million units. The dosages were categorized in each 0.5, 1.0 and 2.0 mg/ml concentration experimental groups. After 2 and 4 days cultures in expeirmental and control groups, the tendency of cell proliferation, MTT assay, protein synthesis ability, collagen synthesis and alkaline phosphatase activity were analysed and bone nodule formation capacity were measured with Alizarin Red S stain after 29 days cultures. The cell proliferation was increased in time, especially the group of 0.5 and 1.0 mg/ml concentration of HA were showed prominent cell proliferation. After 2 and 4 days culture, experimental groups in general were greater cell activity in MTT assay. The protein synthesis was increased in all experimental groups compared to control group, especially most prominent in 1.0 mg/ml concentration group. The collagen synthesis capacity were increased in HA experimental groups, especially prominent in 1.0 mg/ml group and the activity of alkaline phosphatase were increased, especially also prominent in 1.0 mg/ml group, compared to control group. Above these, the activity of mouse carvarial pre-osteoblast cells was showed greater bone osteogenesis activity in all applied HA experimental group, especially group of 1.0 mg/ml concentration of HA.

고삼추출물이 치은섬유아세포의 세포주기 조절단백질 발현에 미치는 영향

김흥식,김현아,유용욱,강태현,김윤철,김탁,피성희,유형근,신형식,Kim, Heung-Sik,Kim, Hyun-A,Yu, Yong-Ouk,Kang, Tai-Hyun,Kim, Youn-Chul,Kim, Tak,Pee, Sung-Hee,You, Hyung-Keun,Shin, Hyung-Shik 대한치주과학회 2000 Journal of Periodontal & Implant Science Vol.30 No.4

Fibroblasts are major cellular components of gingiva and periodontal ligament. They regulate the healing process after surgery or injury. Recently, many natural medicines, whose advantages are less side effects and possibility of long-term use, have been studied for their capacity, their anti-bacterial and anti-inflammatory effects and regenerative potential of periodontal tissues. Sophorae radix have been traditionally used as an anti-bacterial and antiinflammatory drug in oriental medicine. The purpose of present study was to investigate the effects of Sophorae radix extract on cell cycle progression and its molecular mechanism in human gingival fibroblasts. Sophorae radix extracts($100{\mu}g/ml$) notably increased cell proliferation and cell activity in the human gingival fibroblasts as compared to non-supplemented controls. There was an increase in the S phase and a decrease in the G1 phase in $100{\mu}g/ml$ of Sophorae radix extracts group as compared to non-supplemented controls. The level of cyclin E and cdk 2 protein in test group was higher than that of control groups. But that of cyclin D, cdk 4, and cdk 6 was not distinguished from controls. The level of p53 protein in test group was lower than that of controls, whereas that of p21 was not different. The level of pRB protein in test group was higher than that of controls, whereas that of p16 was lower. These results indicate that the increase of cell proliferation by Sophorae radix extracts may be due to the increased expression of cyclin E and cdk 2, and the decreased expression of p53 and p16 in human gingival fibroblasts.

감초산이 인체 치은 섬유모 세포에 미치는 영향

유수경,김기영,유용욱,장선일,김강주,박종운,정종평,Yoo, Soo-Kyoung,Kim, Ki-Young,You, Yong-Ouk,Jang, Seon-Il,Kim, Kang-Ju,Park, Jong-Keun,Chung, Chong-Pyoung,Kurihara, Hidemi 대한치주과학회 1998 Journal of Periodontal & Implant Science Vol.28 No.3

감초산이 인체 치은 섬유모세포에 미치는 영향을 세포의 성장과 증식, 총 교원질 합성 및 인체 치은 섬유모세포 핵내 acridine orange 결합으로 추적조사하였다. 조절이 되지 않는 성장을 해결하기 위하여 세포분화인자인 감초산이 배양 치은 섬유모세포의 활성에 미치는 효과를 검색하였다. 감초산 존재하의 배양 인체 섬유모세포의 세포성장 및 증식, 교원질 합성 및 세포 핵내 acridine orange 결합을 각각 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT)법, 4-hydroxyproline, 유식세포분석기를 이용한 acridine orange 결합으로 검색하였다. 형태학적으로 $100\;{\mu}g/ml$의 감초산으로 처리한 섬유모세포는 모양이 둥글게 되었다. 감초산은 $50\;{\mu}g/ml$ 이상의 농도에서 치은 섬유모세포의 성장과 증식을 억제하였다. 감초산 존재 시에 세포내 총 교원질 양이 감소하였고, 세포외배지내의 교원질 총 양이 증가하였다. 인체 치은 섬유모 세포를 $100\;{\mu}g/ml$의 감초산과 함께 24 시간동안 배양하였을 때, 80 채널 이상의 평균형광을 갖는 diploid 세포가 감소하였고, 80 채널 이하의 형광을 갖는 acridine orange결합이 증가하였다. 이러한 연구 결과 감초산은 인체 섬유모세포에서 세포성장 및 증식, 교원질합성 및 DNA 분절화를 유도함이 제시하였다.

대한치과마취과학회지 게재 논문들의 핵심용어와 MeSH 용어의 일치도

심연수,김아현,유용욱,김일호,류송이,이광석,정채율,김은희,맹선우,안소연,Shim, Youn-Soo,Kim, Ah-Hyeon,You, Yong-Ouk,Kim, Il-Ho,Yu, Song-Yi,Lee, Kwang-Seok,Jeong, Chae-Yul,Kim, Eun-Hee,Maeng, Sun-Woo,An, So-Youn 대한치과마취과학회 2014 Journal of Dental Anesthesia and Pain Medicine Vol.14 No.3

Background: The purpose of this study was to analyze the equality between key words used in the Journal of Korean Dental Society of Anesthesiology and Medical Subject Headings (MeSH). Methods: A total of 666 English key words in 187 papers (average 3.5 words in a paper) from 2001 to 2014 were eligible for this study. We classified them according to matched, and non-matched terms. After descriptive analysis, we assayed patterns of errors in using MeSH, and reviewed frequently used non-MeSH terms. Results: Fifty nine point six percent (59.6%) of total key words were completely coincident with MeSH terms, 40.39% were not MeSH terms. Conclusions: The results show that the coincidence rate of key words with MeSH terms was at a moderate level. However, there is a need for us to understand MeSH more specifically and accurately. Use of proper key words aligned with the international standards such as MeSH is important to be properly cited. The authors should pay attention and be educated on correct use of MeSH as key words.

유향 추출물이 MC3T3-E1 세포 활성 및 분화에 미치는 영향

한상헌,김명동,유승한,유용욱,유형근,신형식,Han, Sang-Heon,Kim, Myoung-Dong,You, Seung-Han,You, Yong-Ouk,You, Hyung-Keun,Shin, Hyung-Shik 대한치주과학회 2001 Journal of Periodontal & Implant Science Vol.31 No.2

Recently, many natural medicines, which have advantage of less side effects and possibility of long-term use have been studied for their capacity of anti-bacterial, anti-inflammatory and regenerative potential of periodontal tissues. Olibanum has the effects to hemostasis, analgesic and anti-inflammatory, and it also has been traditionally used as a drug for the treatment of bone disease in oriental medicine. The purpose of the present study was to investigate the effects of Olibanum extracts on the activity and differentiation of MC3T3-E1 cells, alkaline phosphatase(ALP) synthesis, formation of bone nodules and expression of type I collagen of MC3T3-E1 cells. To examine the cellular activity, MC3T3-E1 cells were cultured with ${\alpha}-MEM(control)$ and each concentration of Olibanum for 2 days and 4 days. To compare the ALP synthesis, MC3T3-E1 cells were cultured with ${\alpha}-MEM(negative\; control)$, dexamethasone(positive control), and each concentration of Olibanum for 2 days and 4 days. To compare the bone nodule formation, MC3T3-E1 ells were cultured for 21 days, and to compare the type I collagen expression, MC3T3-E1 cells were cultured for 4 days. The cellular activity of MC3T3-E1 cells treated with $1{\mu}g/ml$ of Olibanum extracts was significantly increased at 4-day(p<0.05) to control. The activity of ALP in MC3T3-E1 cells treated with $1{\mu}g/ml$ Olibanum extracts was significantly increased at 4-day(p<0.05). All the experimental groups showed much more bone nodule formation than control groups. The group treated with $1{\mu}g/ml$ of Olibanum extracts was the highest bone nodule formation, and showed much more type I collagen expression than negative control. These results indicate that Olibanum extracts may be considered effective in the activity and differentiation of MC3T3-E1 cells.

치은섬유아세포의 복제노화가 세포주기 조절에 미치는 영향

박영채,양대승,김재호,김현아,유용욱,신형식,Park, Young-Chae,Yang, Dae-Seung,Kim, Jae-Ho,Kim, Hyun-A,You, Yong-Ouk,Sin, Hyung-Shik 대한치주과학회 2001 Journal of Periodontal & Implant Science Vol.31 No.1

Gingival fibroblasts are major cellular component of gingiva. However, the molecular mechanisms of senescence of human gingival fibroblasts are unknown. Human fibroblasts undergo replicative senescence in vitro after a limited number of population doublings. A reduced rate of proliferation is a prominent phenomenon observed in senescent fibroblasts. This phenomenon is controled by cell cycle regulatory proteins. The purpose of present study was to investigate the effect of replicative senescence on cell cycle progression and to find out its molecular mechanisms in human gingival fibroblasts. Replicative senescence of gingival fibroblasts were induced by subsequent cultures that were repeated up to 18 passage. In the present study, I examined change of cell proliferation, cell activity, cell viability and cell cycle progression during the replicative process. Also, I examined expression of cell cycle regulatory proteins which was estimated by western blot analysis. Cell proliferation, cell activity and cell viability of gingival fibroblasts were notably decreased with increase of population doubling level(PDL). S phase was decreased and G1 phase was increased with increase of PDL. Western blot analysis showed that levels of P16, p21 and p53 of senescent gingival fibroblasts(PDL41, PDL58) were higher than young fibroblasts(PDL27) and cdk4 were lower than young fibroblasts(PDL27). In conclusion, these results suggest that proliferative function of human gingival fibroblasts may be decreased by replicative senescence and its molecular mechanisms may be activatied with p16, p21, p53 and pRB, and repressed wtih cdk4.

Cyclosporin A가 치은섬유아세포의 세포주기조절에 미치는 영향

피성희,김대겸,김탁,유용욱,유형근,신형식,Pi, Sung-Hee,Kim, Dae-kyum,Kim, Tak,You, Yong-Ouk,You, Hyung-Keun,Shin, Hyung-Shik 대한치주과학회 2001 Journal of Periodontal & Implant Science Vol.31 No.3

Cyclosporin A is a cyclic polypeptide produced by the metabolism of fungi. It is widely used at present as immunosuppressive treatment following organ transplants. It is also used to deal with autoimmune diseases such as rheumatoid arthritis or type II diabetes. Gingival hyperplasia is one of the most frequent side-effects associated with the prescription of Cyclosporin A. The mechanisms involved in Cyclosporin A induced gingival hyperplasia are not yet clear. In vitro Cyclosporin A promotes proliferation of gingival fibroblasts, that Cyclosporin A act as a mitogen. Its action is based on mitosis of gingival fibroblasts regulated by cell cycle regulatory proteins. It was the purpose of the present study to examine the effects of Cyclosporin A on human gingival fibroblasts by means of biological and biochemical criteria. In this present study, we examined change of cell proliferation, cell activity, cell viability and cell cycle progression after application of Cyclosporin A. We also examined expression of cell cycle regulatory proteins by western blot analysis. Human gingival fibroblasts were cultured for 48 hours with application of Cyclosporin A at concentrations of 0.01, 0.1, 1, and 10 ng/ml. Cyclosporin A(1 ng/ml) significantly increased the cell activity of gingival fibroblast. Proliferation and viability of gingival fibroblasts were also increased in group treated with 1 ng/ml of Cyclosporin A compared to control group. In the cell cycle analysis, S phase was increased and G1 phase was decreased in the group treated with 1 ng/ml of Cyclosporin A. Cyclosporin A increased the expression of cdk4 and inhibited the expression of pRB and p21. These results suggest that 1 ng/ml of Cyclosporin A may increase the cell cycle progression of human gingival fibroblasts, and its mechanisms may increase the expression of cdk4 and decrease the expression of pRB and p21.