에탄올 농도에 따른 미나리 추출물의 영양성분 변화

원범영(Beom Young Won),신기영(Ki Young Shin),하현지(Hyun Jee Ha),윤여상(Yeo Sang Yun),김예리(Ye Ri Kim),이형근(Hyung Gun Lee) 한국식품영양과학회 2015 한국식품영양과학회지 Vol.44 No.6

본 연구는 미나리의 최적 추출 조건을 확립하기 위해 다양한 에탄올추출 농도가 미나리의 영양학적 성분 변화에 미치는 정도를 관찰함으로써 진행되었다. 본 실험에서는 열수추출물, 에탄올추출물(50%, 80%, 95%)로 추출 농도를 다르게하여 미나리를 추출하였다. 수율을 측정한 결과 50% 에탄올 추출물에서 44.67%로 가장 높게 나타났다. 일반성분 분석결과, 50% 에탄올추출물에서 단백질 함량이 6.76%, 탄수화물 함량이 19.60%로 가장 높게 나타났다. 지방 함량은 열수추출물과 비교하여 에탄올추출물에서 유의적으로 증가하였으며(P<0.05), 에탄올추출 농도에 따라 유의차가 있었다(P<0.05). 식이섬유를 측정한 결과, 50% 에탄올추출물을 제외한 에탄올추출물(80%, 95%)에서 열수추출물과 유의차가 나타났으며(P<0.05), 두 실험군 간에도 유의적 차이가 있었다(P<0.05). 총당 함량을 측정한 결과, 열수추출물에 대해 에탄올추출물은 통계적 유의차가 있었고(P<0.05), 에탄올 농도에 따라서도 유의차를 나타냈으며(P<0.05), 95%에탄올추출 시 5.35%로 가장 낮게 측정되었다. 비타민 A함량은 열수추출물에 대해 에탄올추출물이 유의하게 증가하였으며(P<0.05), 에탄올추출 농도에 따라 유의차가 있었다(P<0.05). 무기성분 측정 결과, 나트륨, 칼슘, 철의 경우 열수추출물에 대해 에탄올추출물이 유의하게 감소하였고(P<0.05), 에탄올추출 농도가 증가함에 따라 유의적으로 감소하였다(P<0.05). 따라서 에탄올추출 방법을 사용 목적에 따라 적용함으로써 미나리를 이용한 우수한 가공식품 개발가능성과 이용률 향상에 도움이 될 것으로 판단된다. This study evaluated the nutritional compositions of dropwort (Oenanthe javanica) extracts depending on the ethanol concentrations. Extractions were performed with hot water, 50% ethanol, 80% ethanol, and 95% ethanol for 4 hours. Changes in yield, as well as total carbohydrate, crude protein, crude fat, total dietary fiber, free sugar, and mineral (Na, Fe, and Ca) contents were investigated. The highest extraction yield of ethanol extracts was 44.67% in 50% ethanol extract of dropwort. Crude protein content reached a maximum of 6.70% while carbohydrate content was highest at 19.6%, in 50% ethanol extract of dropwort. Crude fat content irregularly increased according to ethanol concentration as compared with hot water extract. Total dietary fiber content decreased in ethanol extract, but these changes were not concentration-related. Total sugar contents were highest in hot water and 80% ethanol extracts. Vitamin A content of ethanol extract was higher than that of hot water extract. Mineral (Na, Ca, and Fe) contents were significantly reduced in ethanol extract according to concentration of ethanol, whereas mineral contents were higher in ethanol extract than in hot water extract. Based on this study, ethanol extract of dropwort is more efficient for development of desirable processed foods.

알츠하이머질병 모델동물인 Tg2576 마우스를 이용한 미나리 알코올추출물의 기억력 개선 효능

원범영(Beom Young Won),신기영(Ki Young Shin),하현지(Hyun Jee Ha),장근아(Keun-A Chang),윤여상(Yeo Sang Yun),김예리(Ye Ri Kim),박용진(Yong Jin Park),이형근(Hyung Gun Lee) 한국식품과학회 2015 한국식품과학회지 Vol.47 No.6

본 연구는 천연 식물이 기억력 개선에 미치는 영향을 검토하기 위하여 총 7가지 식물에 대하여 아세틸콜린분해효소 활성 억제력을 측정하였다. 특히 미나리 알코올추출물(18.76%)의 억제력이 가장 우수하였으며, 미나리 알코올추출물에 대한 추가 연구를 수행하였다. Tg2576 마우스의 기억력에 미치는 영향을 검토하기 위하여 미나리 알코올추출물 50 mg/kg으로 3개월간 경구투여 후 수동회피테스트로 인지기능변화를 측정하였고, 뇌 속의 아세틸콜린분해효소 활성 억제, 베타아밀로이드1-42 단백질 생성 억제력을 측정하였다. 그 결과, 수동회피 테스트에서 미나리추출물을 투여한 Tg 마우스군은 181.77초로 생리식염수를 투여한 Tg 마우스군과 비교하여 머무름 시간이 유의적으로 증가하게 나타났다. 베타아밀로이드1-42 단백질 농도 측정 시 미나리 알코올추출물에 의하여 축적 농도가 985.19 pmol/g으로 감소하였으며, 생리식염수를 투여한 Tg 마우스군과 유의적 차이가 있었다. 추가적인 효소억제력 실험 결과, 미나리 알코올추출물의 아세틸콜린분해효소 활성억제에 대한 50% 활성억제농도(IC<SUB>50</SUB>)값은 991.77 μg/mL로 나타났으며, Lineweaver-Burk Plot 결과, 무경쟁적 저해로 나타났다. 따라서 미나리 알코올추출물은 Tg2576 형질전환 마우스의 인지기능을 개선시키며, 콜린성 신경시스템을 보호하는 물질로 판단된다. 미나리 알코올추출물은 기억 및 학습 증진에 효과적으로 작용하는 천연식물로써 이상의 결과를 근거로 한 미나리 소재의 다양한 기능성제품 개발과 부가가치 향상이 가능할 것으로 판단된다. This study was conducted to investigate the effect of herbs on memory improvement by focusing on their cholinergic functions in Tg2576 mice. Seven herbs were used to obtain extracts by using alcohol and water. In screening test for cholinergic activities of the extracts, acetylcholinesterase (AChE) activity was highly inhibited in Oenanthe javanica alcohol extract (OJAE, 18.76%) as compared with the others. The OJAE-treated Tg2576 (Tg-OJAE) groups showed the statistically significant increases of latency time in passive avoidance test. Also, it was found that the concentration of Aβ1-42 was significantly reduced in Tg-OJAE groups compared to non-treated Tg2576 groups. In the additional enzyme test, it was found that IC<SUB>50</SUB> of OJAE was 991.77 μg/mL and OJAE acted as an uncompetitive inhibitor of AChE. Therefore, it seemed that OJAE can be used for the development of processed foods for memory improvement.

미나리 추출물의 기억력 손상 억제와 산화스트레스 억제 효과 및 Isorhamnetin 분석

원범영(Beom Young Won),신기영(Ki Young Shin),하현지(Hyun Jee Ha),위지향(Ji-Hyang Wee),윤여상(Yeo Sang Yun),김예리(Ye Ri Kim),박용진(Yong Jin Park),정경옥(Kyoung Ok Jung),성혜미(Hea Mi Sung),이형근(Hyung Gun Lee) 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.1

본 연구는 한국인이 일상생활에서 주로 상용하는 미나리에 대해 기억력 관련 효과와 항산화 효과, 미나리 추출물의 flavonoid 중 isorhamnetin을 분석하여 기능성식품 개발 및 이용에 기여하고자 실시하였다. Acetylcholinesterase의 활성 억제를 측정한 결과 화순미나리 추출물이 28.59%로 가장 높게 나타났으며, 나주미나리 추출물 25.11%와 유의적인 차이가 없었다. SH-SY5Y 세포사멸에 미치는 영향을 측정한 결과 DMSO와 비교하여 화순미나리 추출물 37.23%와 나주미나리 추출물 36.68%로 세포사멸이 유의적으로 감소하였다. 수동회피실험 결과 scopolamine 처리에 의해 기억력이 손상된 동물모델에서 미나리 추출물을 투여하였을 때 vehicle control보다 latency time이 높게 나타났다. DPPH 및 ABTS 라디칼 소거능 측정 결과 화순, 나주, 의령 미나리 추출물 모두 미나리 처리 그룹군 간에도 유의적 차이가 있었고(P<0.05), 화순미나리 추출물이 총 폴리페놀 함량 117 mg/g, 총 플라보노이드 함량 30 mg GAE/g으로 가장 높게 측정되었다. TBARS 측정 결과 미나리 추출물 440μg/mL의 농도에서 화순미나리 추출물이 50.7%의 억제력으로 가장 높았으며, 나주와 의령미나리 추출물보다 유의하게 높았다. 미나리 추출물의 플라보노이드 중 isorhamnetin을 분석한 결과 표준용액과 미나리 추출물의 머무름 시간이 14분대로 피크 유지시간이 일치하였으며, 동일한 spectrum을 나타내어 정확한 isorhamnetin의 분리를 확인하였다. 또한 LC/MS/MS를 통한 분석 결과 표준용액과 미나리 추출물에서 동일 시간대에 TIC를 확인하였고 precursor ion은 317 [M-H]+ m/z, product ion은 302 [M-H]+ m/z로 확인할 수 있었다. 이러한 연구 결과에 기초하여 미나리추출물은 천연 항산화제와 기억력 개선제로써의 활용 가치를 시사한다고 사료된다. 또한 건강기능성 소재로서 식품산업 분야의 이용률 향상에 도움이 될 것으로 판단된다. This study was conducted to investigate effect of the acetylcholinesterase inhibitor activity, the protective effect of the extract on SH-SY5Y cell death by H₂O₂, the memory improvement from scopolamine-induced rat. Moreover, the antioxidant activity of isorhamnetin from the dropwort (Oenanthe javanica) was investigated. Acetylcholinesterase inhibitor activity was highest (28.59%) in Hwasun O. javanica extract (H-OJE). H-OJE and Naju O. javanica extract (N-OJE) were not significantly different. SH-SY5Y cell death deceased to 37.23% and 36.68% for H-OJE and N-OJE, respectively, following treatment with the extracts. O. javanica extracts showed a protective effect against H₂O₂-induced neurotoxicity. Treatment with O. javanica extracts slightly improved scopolamine-induced (1 mg/kg, i.p.) memory impairment in rats. H-OJE contained the highest total phenolic and flavonoid contents of 117 mg/g and 30 mg gallic acid equivalents/g, respectively, and had a DPPH radical scavenging activity (SC50) of 113.8 μg/mL and ABTS radical scavenging activity of 48.2 μg/mL, which was higher than the other extracts. The thiobarbituric acid reactive substances value was highest (50.2%) in H-OJE. Antioxidant activity differed significantly among dropwort extracts. Isorhamnetin was known as one of the flavonoid and for having neuroprotective effect. So we analyzed acid-hydrolyzed O. javanica extract HPLC. The results were that peak at 14 min and spectrum of the extracts was consistent with standard solution. The results of LC/MS/MS analysis were that the extract and standard solution were confirmed total ion chromatogram at identical time, precursor ion was 317 [M-H]+ m/z, product ion was 302 [M-H]+ m/z. Overall, the results showed that the dropwort extract led to memory improvement and had antioxidant activity. Based on these finding, further research to investigate the production of ethanol extract of dropwort as a processed food is warranted.

미나리의 기억력 증진 효과에 대한 연구

신기영,위지향,원범영,이형근 한국약용작물학회 2013 한국약용작물학회 학술대회논문집 Vol.2013 No.1

미나리의 기억력 증진 효과에 대한 연구

신기영,위지향,원범영,이형근 한국약용작물학회 2013 한국약용작물학술대회 발표집 Vol.2013 No.05

Ginsenoside Re Enriched Fraction (GS-F3K1) from Ginseng Berries Ameliorates Ethanol-Induced Erectile Dysfunction via Nitric Oxide-cGMP Pathway

표미경,박광현,오명환,이환,박영식,김나영,박소희,송지혜,박종대,정세희,이봉건,원범영,신기영,이형균 한국생약학회 2016 Natural Product Sciences Vol.22 No.1

Erectile dysfunction (ED) is a highly prevalent disorder that affects millions of men and considered to be an early symptom of atherosclerosis and a precursor of various systemic vascular disorders. The aim of the present study was to prepare ginsenoside Re enriched fraction (GS-F3K1, ginsenoside Re 10%, w/w) from ginseng berries flesh and to investigate the enhanced activities of GS-F3K1 on alcohol-induced ED. GS-F3K1 was prepared by the continuous liquid and solid separating centrifugation and circulatory ultrafiltration from ginseng berries flesh. GS-F3K1 was administered for 5 weeks in ethanol-induced ED rat by oral administration of 20% ethanol. To investigate the effects of GS-F3K1 on ED model, the levels of nitrite expression, cyclic guanosine monophosphate (cGMP) and erectile response of the penile corpus cavernosum of rat were measured. The erectile response of the corpus cavernosum was restored after GS-F3K1 administration, to a level similar to the normal group. The level of nitrite and cGMP expression in the corpus cavernosum of GS-F3K1-administered male rats was increased significantly compared to positive control group. GS-F3K1 from ginseng berries should effectively restore ethanol-induced ED in male rats and could be developed as a new functional food for the elderly men.

한국 노인의 낙상 실태와 위험요인: 일부 지역의 인구비례 할당 표본 조사

임재영,박원범,오민균,강은경,백남종 대한노인병학회 2010 Annals of geriatric medicine and research Vol.14 No.1

Background: To investigate the occurrence of falls and their consequences in a representative population and to identify risk factors of falls in the elderly. Methods: The study participants were community-dwelling elderly people aged 65 years or old as a population based sample of one large city. A total of 828 people 65 years or older participated in this study. They were interviewed over the telephone by a trained interviewer using a structured questionnaire to obtain a fall history and details of their most recent fall including date, time, place, circumstances, and associated injury. As for risk factors for falls, we asked about living arran- gement(with family or alone), alcohol intake, exercise, visual and hearing impairments, memory and sensory problems, lower limb weakness, and arthritis symptoms. Fear of falling and fear-related activity restriction were also evaluated. Results: The incidence of falls during the last one year was 13.0%. Women experienced falls more frequently. Falls occurred mainly in spring and summer and from 10 AM to 3 PM. Common causes of falls were slipping and tripping. 14.9% of those who fell suffered consequent fractures. Being female was a significant risk factor for falls; and females tended to get injured more severely following a fall. During the recent one year, decreased proprioception in the lower extremity was a significant risk factor for falls. Those with histories of falls, and especially women, tended to limit their activities due to the fear of falling. Conclusion: Overall, it appears that elderly women are more greatly affected by falls at several levels. Female partici- pants fell more frequently and suffered more severe consequences. Fall-related injuries and risk factors for falls disproportionately affected women. And, being female and any amount of decreased sensation in the feet were significant risk factors for falls. 연구배경: 일부 지역의 인구 비례할당 표본 조사를 통하여노인의 낙상의 발생과 그 결과를 조사하고 낙상 위험 인자를확인하고자 하였다. 방법: 한 지역의 65세 이상 인구의 비례할당 표본을 대상으로 숙련된 조사요원에 의한 전화조사를 통해 낙상발생 날짜,시간, 장소, 상황 등에 대하여 조사하였다. 그리고 관련된부상 등 낙상 결과에 관한 조사와 낙상에 대한 위험인자,즉 독거 유무, 알코올섭취량, 운동량, 청각과 시각장애, 기억력, 감각 문제, 하지 위약 및 관절염 증상 등을 물어보았다. 또한 낙상의 두려움과 두려움 관련 활동제한도 평가하였다. 결과: 지난1년 동안 낙상의 발생률이 13%였고, 여성의발생률이 더 높았다. 낙상은 주로 봄이나 여름 10시~3시사이에 주로 나타났다. 낙상의 일반적인 이유는 미끄러짐과걸려 넘어짐이었다. 14.9%의 사람이 골절상을 입었다. 여성이 낙상의 중요한 위험요소였는데, 여성의 경우 더 심각하게부상을 나타내는 경향이 있었다. 최근 1년 동안 낙상의 주된위험요소로는 하지의 고유수용체감각의 감소였다. 특히 여성에서 낙상에 대한 두려움 때문에 활동을 제한하는 경향을보였다. 결론: 노인에서 여성 자체가 낙상의 위험인자이다. 여성노인이 더 자주 넘어지고 낙상 후에 더 나쁜 낙상 결과를갖게 되며, 낙상 관련 두려움과 이로 인한 활동제한도 더많았다.

한국 일부 지역 노인들에서 균형 자신감과 신체활동 능력과의 관계

박원범,임재영 대한노인병학회 2009 Annals of geriatric medicine and research Vol.13 No.4

Background: To investigate the physical performance and changes in function by age and sex in community dwelling elderly and to assess the associations between balance confidence and physical performance and functioning. Methods: We recruited 180 people aged 65 years or older capable of independent ambulation and activities of daily living. They were divided into three groups according to age: 65-74 years, 75-84 years and 85 years or older, to assess the differences in physical functioning between the groups. Short physical performance battery(SPPB), activity based balance confidence(ABC) and hand and thigh strengths were measured as variables of physical performance. Pain related disabilities and body composition were also included in the analysis. Results: SPPB scores in women decreased more than that of men in those younger than 85 years, but this difference was not significant for those older than 85 years. ABC scores showed the same decreasing pattern, and sex diffe- rence continued in the oldest group. For pain related disabilities, a remarkable gender gap was identified. Hand and thigh strengths decreased with age, with the more prominent decrease seen in men than in women from the older age group. Although most variables correlated with the physical performance evaluated by the SPPB, only the ABC significantly influenced the SPPB after controlling for the covariates. Conclusion: Balance confidence in the specific activity plays a major role in physical performance and function.

한국 일부 지역 노인들의 체력 및 신체 활동이 삶의 질에 미치는 영향

김경은,박원범,오민균,강은경,임종엽,양은주,임재영,백남종 대한노인병학회 2010 Annals of geriatric medicine and research Vol.14 No.4

연구배경: 한국 일부지역의 코호트에서 객관적 정량화된도구를 이용하여 한국 노인의 체력과 신체 기능에 대해 조사하고 삶의 질과의 관계를 평가하고자 하였다. 방법: 성남시 65세 이상 노인에서 구축한 1,000명의 코호트 중 676명에서 슬관절 신전근, 굴곡근의 등속성 근력 검사,수부 악력검사, 신체활동능력평가(Short Physical Performance Battery, SPPB), 신체 활동 정도 평가 및 삶의 질 평가를시행하였다. 각각을 성별 및 연령에 따라 65-74세 중고령노인(old-old), 75-84세 고령 노인(older-old), 85세 이상 초고령 노인(oldest-old)으로 분류하여 분석하였다. 또한 각 신체기능 검사 결과와 삶의 질과의 관련성을 파악하기 위하여상관분석 및 다중선형회귀분석을 시행하였다. 결과: 슬관절 신전근 및 굴곡근의 등속성 근력 및 수지악력은 남녀 모두 연령의 증가에 따라 유의하게 감소하였다. SPPB 및 신체활동 정도, 삶의 질 척도 중 신체적 건강지수는모두 여성에서는 고령기, 초고령기에 지속적으로 감소하였으나 남성에서는 중고령기, 고령기에 비슷하게 유지되었다. 신체적 건강관련 삶의 질에 영향을 미치는 인자로 SPPB 및 신체활동 정도는 남녀 모두에서 유의하였으나, 하지 근력은 여성에서만 유의한 인자로 나타났다. 결론: 노인의 신체기능 및 신체활동 정도는 성별에 따라다르게 감소하였으며, 삶의 질과 연관된 주요 인자로 나타났다. Background: To investigate the physical function of elderly people in Korea using quantitative and objective tools and to assess the impact of declined physical function on their quality of life. Methods: The Korean Longitudinal Study on Health and Aging was designed as a population-based prospective cohort study on the health and aging of the elderly people in Korea aged 65 years and older. Within one city’s central metropolitan area, 1,000 subjects including a simple random sample and an oldest-old subpopulation participated in a baseline study for one year. We evaluated the isokinetic peak torque of the knee extensors and flexors, grip force, and Short Physical Performance Battery(SPPB) and surveyed their daily physical activities and quality of life using the 36-Item Short Form Health Survey(SF-36). Results: Overall, 676 subjects completed the evaluations of physical function and activity. The isokinetic torque of the knee extensors and flexors declined with age in both men and women. The women in the old-old(75-84 years) group showed lower SPPB and physical activity than women in the young-old(65-74 years) group, but these measures were maintained till age 85 in the men. SPPB and physical activity were significantly associated with the physical component scale (PCS) of the SF-36 in both men and women, while isokinetic torque was associated with PCS in women only. Conclusion: Physical performance and activity declined differently in Korean elderly men and women. Both these measures showed to be the main factors associated with quality of life.

통합적 노인 낙상 예방 프로그램의 장단기 효과

임종엽,임재영,박정아,오민균,박원범,강은경,신형익,백남종 대한재활의학회 2010 Annals of Rehabilitation Medicine Vol.34 No.4

Objective: To evaluate the short-term and long-term effects of integrated fall prevention program comprised of exercise program to improve physical function, education of fall prevention strategy and environmental improvement in elderly Korean. Method: Elderly who were at high risk for fall participated in 8-week integrated fall prevention program. Outcome measures including fall efficacy scale (FES), activity-specific balance confidence (ABC), flexibility test, balance function test, short physical performance battery (SPPB) and isokinetic knee strength were evaluated and compared before and after the program. One year after the program, fall experience and frequency, FES and ABC were followed up by mail questionnaire. Results: Among 63 participants enrolled at the baseline, 43 completed the program. Flexibility, balance function and SPPB improved significantly whereas FES, ABC, anthropometric measure data and knee strength were not changed. One year after the program, the number of fallers decreased significantly. Conclusion: Integrated fall prevention program may improve flexibility and balance function, and can reduce the risk of fall in the elderly population. (J Korean Acad Rehab Med 2010; 34: 451-457) Objective: To evaluate the short-term and long-term effects of integrated fall prevention program comprised of exercise program to improve physical function, education of fall prevention strategy and environmental improvement in elderly Korean. Method: Elderly who were at high risk for fall participated in 8-week integrated fall prevention program. Outcome measures including fall efficacy scale (FES), activity-specific balance confidence (ABC), flexibility test, balance function test, short physical performance battery (SPPB) and isokinetic knee strength were evaluated and compared before and after the program. One year after the program, fall experience and frequency, FES and ABC were followed up by mail questionnaire. Results: Among 63 participants enrolled at the baseline, 43 completed the program. Flexibility, balance function and SPPB improved significantly whereas FES, ABC, anthropometric measure data and knee strength were not changed. One year after the program, the number of fallers decreased significantly. Conclusion: Integrated fall prevention program may improve flexibility and balance function, and can reduce the risk of fall in the elderly population. (J Korean Acad Rehab Med 2010; 34: 451-457)