Complete genome sequence of Pediococcus acidilactici BB2-4M isolated from cattle faeces

원미영,이다슬,한병학,홍승범,권순우,허준 한국미생물학회 2022 미생물학회지 Vol.58 No.3

Pediococcus acidilacti BB2-4M, isolated from cattle faeces, is potential feed additives to reduce methane gas, improve digestion and inhibit pathogen in the rumen. The genome of strain BB2-4M consists of a circular chromosome (2,040,105 bp) and one plasmid (60,691 bp), with DNA G + C content of 42.0%. The complete genome of strain BB2-4M was identified to have 2,009 genes, including cellulose synthase enzyme and oligopeptide transport proteins. This study will also provide genomic information on oligopeptide transport proteins (opp operon) that can be compared with the Pediococus acidilacti DSM 20284T.

다양한 식물병원균에 대한 길항능을 지닌 토양 세균 Bacillus subtilis BC1-43 균주의 유전체 염기서열

원미영,문혜정,상미경,송재경,원항연 한국미생물학회 2021 미생물학회지 Vol.57 No.4

A Bacillus subtilis strain BC1-43 was isolated from soil, which demonstrated antifungal activity against a wide range of phytopathogenic fungi. The genome of strain BC1-43 was sequenced using the PacBio Sequel system. The draft genome was 4,185,798 bp (chromosome 4,157,766 bp and plasmid 28,032 bp) and the genomic G + C content was 43.6%. In total, the number of coding sequences was 4,170, including 30 rRNAs and 86 tRNAs. We identified gene clusters encoding antimicrobial compounds (bacilysin, subtilosin, bacillibactin, fengycin, bacillaene and surfactin), which are involved in the suppression of phytopathogenic fungi. 토양에서 분리한 Bacillus subtilis BC1-43 균주는 다양한 식물병원균에 대한 길항능을 가지고 있었다. 이 균주의 유전체는 4,157,766 bp 크기의 염색체와 28,032 bp 크기의 플라스미드로 구성되었다. 유전체 초안으로부터 단백질 유전자 4,1708 개, rRNA 유전자 30개, tRNA 유전자 86개를 확인하였다. 유전자 분석 결과 Bacillus BC1-43은 antifungal compounds인bacilysin, subtilosin, bacillibactin, fengycin, bacillaene 및surfactin과 같은 다양한 2차 대사 산물을 생산하는 유전자가 확인되었다. Bacillus subtilis BC1-43 균주의 유전체 분석은 식물병원균의 생물적 방제 기작을 이해하는 데 기여할것이다.

가열온도, 가열시간 및 부형제의 첨가량이 도축 반추위 내용물의 자일란, 셀룰로오스 및 전분 분해효소 활성에 미치는 영향

원미영,이도형,김은중,Won, Mi Young,Lee, Do Hyung,Kim, Eun Joong 한국초지조사료학회 2013 한국초지조사료학회지 Vol.33 No.1

본 연구는 도축장에서 폐기되는 도축 반추위 내용물을 사일리지 혹은 TMR (total mixed ration) 사료 첨가용 효소제로 개발하기 위한 목적으로 수행되었다. 도축 반추위 내용물에는 상당한 수분이 함유되어 있어, 적절한 건조과정을 거치지 않고서는 그 활용이 원활하지 않다. 그러나 효소는 열에 민감한 단백질로 구성되어 있기 때문에 적절한 건조 조건의 개발이 필요하다. 본 연구에서는 통계적 방법을 이용하여 가열온도(60, 75, $90^{\circ}C$), 가열시간(12, 30, 48 시간) 및 부형제의 비율(12, 22.5, 33%)이 도축 반추위 내용물에 함유된 다양한 효소활성에 미치는 효과를 분석하였다. 총 3가지 효소, xylan 분해효소, 셀룰로오스 분해효소 및 전분 분해효소를 검토하였고, 각 효소활성들에 대한 각 요인들의 효과가 매우 다양하게 나타났다(p<0.05). 셀룰로오스 분해효소와 전분 분해효소의 활성은 가열온도가 증가함에 따라 감소하였으며(p<0.05), xylan 분해해소는 열에 대한 안전성이 다른 효소들에 비하여 우수하였다. 자일란, 셀룰로오스, 전분 분해효소를 증가시키는 적정 부형제의 비율은 22.5, 12 그리고 33%로 나타났다. 비록 3가지 효소들의 제형화에 있어 공통적으로 적용될 수 있는 최적점을 도출하지는 못하였으나, 본 연구결과에서 얻어진 효소들의 반응 값들을 이용하여 목적하는 효소에 따라서 다양한 방법으로 적용이 가능할 것으로 판단된다. This study was conducted in order to develop slaughterhouse rumen content (SRC) as a potential feed additive. The moisture content of SRC can reach 80%, and therefore an appropriate dewatering process is required before it can be used. In this study, the effects of heating temperature, heating time, and filler content during the dewatering process on the activity of various enzymes in SRC were investigated. The Box-Behnken experimental design was employed, involving a total of 45 experimental runs, consisting of three variables (heating time, heating temperature, and filler content) with three levels per variable (12, 30 and 48 hr; 60, 75 and $90^{\circ}C$; 12, 22.5 and 33% for heating time, heating temperature, and filler content, respectively). For enzyme activities, xylanase, cellulase, and amylase were examined, and the results were subjected to an analysis of variance. Heating time, heating temperature and filler content had significant effects on the activity of each enzyme (p<0.05). Cellulase and amylase activities decreased (p<0.05) at elevated heating temperatures, whereas xylanase was reasonably stable around $90^{\circ}C$. The activities of all enzymes decreased (p<0.05) with increased heating time. Optimum filler contents for xylanase, cellulase, and amylase activities were 22.5, 12 and 33%, respectively. However, optimum conditions for all variables that simultaneously maximize the activity of all three enzymes could not be ascertained in this study. Nevertheless, the results from the current study can be useful as basic information for the development of SRC as a feed additive enriched with improved major enzymes for livestock feed digestion.

책읽기 프로그램이 부모-유아의 상호작용과 발달지체 유아의 의사소통 행동에 미치는 영향

원미영,박송희,고유선 한국유아특수교육학회 2016 한국유아특수교육학회 학술대회 논문집 Vol.2016 No.3

중합효소반응을 이용한 마렉병 바이러스의 병원성 분류

원미영, 권혁준, 모인필 충북대학교 동물의학연구소 2003 Journal of Biomedical and Translational Research Vol.4 No.2

-본문참고-

폴리페놀을 다량 함유한 커피박 및 녹차박 추출물의 수준별 첨가가 반추위 발효 및 메탄 발생량에 미치는 영향

원미영 ( Won¸ Miyoung ),류채화 ( Ryu¸ Chae-hwa ),박혜련 ( Bak¸ Hyeryeon ),채병호 ( Chae¸ Byungho ),장승호 ( Jang¸ Seung-ho ),최승신 ( Choi¸ Seung-shin ),최봉환 ( Choi¸ Bong-hwan ),이성수 ( Lee¸ Sung-soo ),이진욱 ( Lee¸ Jinwook 한국유기농업학회 2021 韓國有機農業學會誌 Vol.29 No.4

This study was conducted to investigate the effect of addition levels of coffee and green tea by products extract including polyphenols through hot water extraction on rumen fermentation. The treatment groups consisted of coffee extract (CO), green tea extract (GR) and mixed extract (MIX), and the addition level was 10 μL, 20 μL and 30 μL of three levels. The experiment consisted of a total of 10 experimental groups including the control group, and a full factorial design was used. The effect of polyphenol addition in coffee and green tea by-products was analyzed through main and interaction effect of statistical analysis. The total polyphenol content of the extracts was 106.15, 79.10 and 185.25 μg GAE/g DM for coffee by-product, green tea by-product and mixture, respectively. Total gas production was significantly lower in the treatment groups than in the control (114.00 mL/gDM) (p<0.05). Methane emission tended to decrease as the polyphenol addition level increased. Moreover, the MIX showed the lowest methane emission when 30 μL was added (p<0.05). Volatile fatty acids showed a significant difference compared to the treatment group as a control (98.06 mM) (p<0.05), but there was no change according to the level of polyphenols. As a result of the main effect and interaction, it is thought that the effect on methane reduction and improvement of rumen fermentation in MIX20 can be expected. In a series of studies, the addition of 20 μL of a blended extract of coffee and green tea by-products is thought to reduce methane to levels that do not inhibit rumen fermentation.

헤어샵 직원에게 지각된 리더상이 직무만족과 이직률에 미치는 영향

원미영 ( Won-mi Young ) 한국미용예술경영학회 2016 미용예술경영연구 Vol.10 No.2

본 연구는 인구통계학적 특성에 따른 미용종사자의 헤어샵 리더의 리더성향에 따른 직원들의 직무만족과 이직률를 알아봄으로써, 리더 성향이 직무만족과 이직률에 어떠한 영향을 미치는가를 검증하고 개선점을 제시하고자 한다. 자료 수집은 수도권지역 미용실에 있는 직원들을 대상으로 최종 300부의 설문지를 SPSS 21.0 통계프로그램을 이용하여 분석하였으며 연구결과, 결론, 제언은 다음과 같다. 지각된 헤어샵 리더 상에 따른 직무만족에 대해 유의한 차이를 보이지 않았지만 리더 상에 따라 이직률을 알아본 결과 영향을 미치는 것으로 나타났고 선행연구에서도 같은 결과가 나왔다. 이러한 결과를 통해 미용사의 직무만족과 이직률의 감소를 위하여 조직에서 리더관리의 중요성을 생각하고 리더의 탁월한 선택과 지휘로 조직을 성공으로 이끌어 가기위한 리더의 노력과 행동으로 여러 가지 해결방법이 될 수 있는 제시는 다음과 같다. 첫째, 관리자가 직원들과 지속적인 대화와 격려를 도모하고 충분한 상담으로 직원과 관리자 서로 간에 노력하는 것이 필요하다. 둘째, 정기적으로 회의를 진행하여 서로의 의견을 모아 공정하게 의견을 정리하여 결정하고 모두에게 발언권을 주어 불만이 없도록 관리자가 주도해 주는 것이 필요 하다. 셋째, 관리자가 솔선수범하는 자세로 모두에게 모범이 되고 존경스러운 행동을 항상 보이려하는 것이 필요하다. There is a connection between the reliance on a leader and the difference between job satisfaction and the turnover rate. This research suggests that the effectiveness of a leader influences job satisfaction and the turnover rate, we want to improve things. Data was collected through a questionnaire and statistics program," sps21.0", from hair salon staff in the Seoul metropolitan area. We cannot find a difference in job satisfaction with this questionnaire, but the reliance on a leader affect the turnover rate. We also see this result in the preceding study. To increase job satisfaction and improve the turnover rate, it is more important to control leadership. I recommend like these steps: First, to decrease the turnover rate the manager must always consult with the staff and encourage them. Second, managers must regularly attend staff meeting, and directly run the hair salon. Third, always try to lead from the front. It is necessary to show a good example to staff.

마렉백신의 In-Ovo 접종에 따른 육계에서의 효능 평가

엄현정,원미영,이동우,고도욱,모인필,Um, H.J.,Won, M.Y.,Lee, D.W.,Go, D.W.,Mo, I.P. 한국가금학회 2006 韓國家禽學會誌 Vol.33 No.4

본 실험은 육계에서의 Marek's disease(MD) 백신에 대한 안전성과 효용성을 국내에 기 도입된 18일령 계란에서의 백신 접종 방법(In-Ovo vaccination)을 이용하여 평가하고자 실시하였다. 육계에서의 MD 오염율은 nested PCR을 이용하였으며 백신 접종군과 비접종군간의 생산성 차이를 출하 일령, 평균출하체중, 출하율, 사료 효율을 기본으로 하는 생산 지수를 측정하여 비교하였다. 백신에 의한 면역 억제 현상을 파악하기 위하여 접종 농장에서 채취한 혈청에서의 항체 역가수준과 F낭의 무게 변화를 검사하였다. 국내 육계에서의 MD 발생율을 파악하기 위하여 nested PCR을 실시한 결과 조사 대상 농장 중 26.09%가 오염되었으며, 백신 접종 농장과 비접종 농장간의 생산 지수의 차이는 통계학적으로 유의한 차이가 없었다. 그러나 MDV 오염군에서의 MD 백신 접종 농장의 생산 지수가 비접종 농장보다 높아 간접적으로 효과가 있음이 인정되었다. 면역 억제여부를 파악하기 위한 검사에서는 두 그룹 간 B/B ratio 차이는 없었으나 두 계군 모두 출하 시 심한 위축을 보여주어 혈청 검사 성적과 연계하여 볼 때 국내 육계 농장에서의 IBD 감염이 만연되어 있다는 것을 알 수 있었다. 본 연구를 통해서 국내 육계에서의 MDV 오염율이 파악되었으며, In-Ovo 백신 접종에 의한 MD 백신 접종은 안전성에 있어서는 별다른 문제가 없었으나 효능면에 있어서는 MD 야외감염율이 낮고 IBD와 같은 면역 억제 질병의 감염이 만연되어 있어 정확한 평가가 되지 않아 앞으로 후속 연구가 진행되어야 할 것으로 판단되었다. This study has been designed to evaluate the efficacy of MD in-ovo vaccination in broiler and to find the contamination level of MD virus in broiler farms. A nested PCR test was used to find the level of filed contamination of pathogenic MD virus in the commercial broiler farms. The positive rate of farms contaminated with pathogenic MD virus was 26.09%. And to evaluate the efficacy of MD vaccination, performance factors such as the mortality, feed conversion rate and body weight at market age has been recorded and compared among farms. There was no difference on these factors between vaccinated and non-vaccinated farms except performance. We need further investigation of the film with low performance index. The B/B ratio, which is common index for the evaluation of bursal changes, was calculated and compared between vaccinated and non-vaccinated group There were significant decreases of B/B ratio in the both group without any statistical difference between groups. The reason of decreased bursal size may be due to other infection such as IBD. From this study, we can know that the contamination rate of fm with pathogenic MD virus was very low and not different from previous study. However, the difference of the contamination rate in different locations recommends periodical monitoring on these areas.

Report of eight unrecorded Acetobacter species in Korea, discovered during the survey in 2018-2019

허준,원미영,이다슬,한병학,홍승범,권순우 국립생물자원관 2022 Journal of species research Vol.11 No.3

Acetic acid bacteria (AAB) convert ethanol to acetic acid through oxidation, and the fermentation pathway of AAB is important in the vinegar industry. The genus Acetobacter is the representative one of AAB, and several Korean traditional vinegars are produced using Acetobacter strains. Until now, four species in the genus Acetobacter were reported as native species in Korea. During the past two years, we isolated several AAB strains from fruits, flowers and fermented foods, and several AAB species unrecorded in Korea were found on the basis of 16S rRNA gene sequence analyses. In this study, we report eight Acetobacter species as native ones which are A. fabarum C10-3 (=KACC 21483) isolated from plumcot fruit (Najusi), A. lovaniensis KDG-EC1 (=KACC 22697) isolated from diced radish kimchi (Naju-si), A. okinawensis GAM12-M2 (=KACC 22696) isolated from persimmon fruit (Sangju-si), A. orientalis FR32C4 (=KACC 22370) isolated from fruit of Cudrania tricuspidata (Jeonju-si), A. papaya FR35B3 (=KACC 22046) isolated from grape fruit (Yeongdong-gun), A. suratthaniensis GAM15-R2 (=KACC 22694) isolated from persimmon fruit (Gimje-si), A. syzygii C25-1 (=KACC 22048) isolated from peach fruit (Namwon-si) and A. thailandicus JDF1-M1 (=KACC 22693) isolated from plum fruit (Seoul).

Differences in fine dust emissions based on bedding type and quantity in horse stables

유지현,이종안,최재영,신상민,김현아,원미영,강용준,지희정,조인철,김진형 충남대학교 농업과학연구소 2023 Korean Journal of Agricultural Science Vol.50 No.4

Efforts for developing the domestic horse industry in South Korea are evident through the various national policies. Proper management of stables for ensuring the health of horses is essential to sustain the growing equine industry. This study aimed to measure the fine dust emissions in stables based on the type and quantity of bedding used for horses, for establishing guidelines pertaining to bedding use in stables. The stables accommodated 12 horses. Sawdust, wood shavings, wood pellet, and straw were chosen as treatments. Three different quantities (approximately 3, 6, and 9 cm in height) were applied for each type. Fine dust measurements were carried out at three time points, with each measuring period lasting for approximately three weeks. Measurements included PM2.5 and PM10 fine dust levels. The initial analysis revealed that, sawdust with 9 cm bedding had the highest dust levels; approximately 54.6 μg/m3, for PM2.5 and 95.3 μg/m3, for PM10. Sawdust bedding at the highest quantity (9 cm) exhibited significantly higher initial fine dust emissions. These findings suggest that bedding materials with smaller particle size, such as sawdust and wood shavings, tend to produce finer dust. Initially, the fine dust emissions decreased in all bedding types and quantities, possibly due to the increased moisture content of bedding owing to horse manure production. However, emissions increased subsequently due to ammonia production.