기계적 자극이 다층 구조의 나노파이버 지지체의 평활근 세포에 미치는 영향

신지원,김동화,허수진,김수향,김영직,신정욱,Shin, Ji-Won,Kim, Dong-Hwa,Heo, Su-Jin,Kim, Su-Hyang,Kim, Young-Jick,Shin, Jung-Woog 대한의용생체공학회 2008 의공학회지 Vol.29 No.1

The object of this study is to investigate the effects of intermittent cyclic stretching on the smooth muscle cells (SMCs) seeded onto aligned multi-layered fibrous scaffold. To make multi-layered fibrous scaffold, polyurethane (PU) and poly(ethylene oxide) (PEO) were electrospun alternatively, then were immersed into distilled water to extract PEO. Various types of scaffolds were fabricated depending on fiber directions, i.e., aligned or randomly oriented. The direction of stretching was either parallel or vertical to the fiber direction for the aligned scaffolds. The stretching was also applied to the randomly aligned scaffolds. The duration of stretching was 2 min with 15 min resting period. During the stretching, the maximum and minimum strain was adjusted to be 10 and 7%, respectively with the frequency of 1 Hz. The bioactivities of cells on the scaffolds were assessed by quantifying DNA, collagen, and glycosaminoglycan (GAG) levels. And the cell morphology was observed by staining F-actin. SMCs under parallel stretching to the fiber direction responded more positively than those in other conditions. From the results, we could explain the morphological effect of a substrate on cellular activities. In addition the synergistic effects of substrate and mechanical stimuli effects were confirmed.

(γ-Aminobutyric acid를 생산하는 Lactobacillus brevis AML15의 분리 및 특성

신지원,김동걸,이용우,이형석,신기선,최충식,권기석,Shin, Ji-Won,Kim, Dong-Geol,Lee, Yong-Woo,Lee, Hyoung-Seok,Shin, Kee-Sun,Choi, Chung-Sig,Kwon, Gi-Seok 한국생명과학회 2007 생명과학회지 Vol.17 No.7

국내해안의 젓갈과 김치류로부터 86종의 GABA 생산균주를 분리하였다. 분리된 균주들을 Thin layer chromatography를 이용하여 GABA 생성능이 우수한 AML15, AML45-1, AML72의 3종의 균주를 선발하였다. 선별된 3종의 균주의 아미노산 분석 결과 GABA 생성능이 가장 우수한 AML15 균주를 본 실험에 사용하였다. AML15의 분류학적 위치를 규명하기 위하여 16S ribosomal DNA 영역의 부분염기서열 분석을 실시하였다. 165 rDNA 분석결과 Lactobacillus brevis ATCC 367과 99%의 유사도를 나타내어 L. brevis AML15로 명명하였다. MRS 배지에 최종 전환 농도로 설정된 5%(w/v) monosodium glutamic acid를 첨가하고 배지의 초기 pH를 4.0, 5.0과 6.0으로 조정하여 배양한 결과 배지의 초기 pH가 5.0일 때 GABA 생성능이 가장 높게 조사되었다. GABA 생산배지에 GAD 효소활성에 조효소로 작용하는 PLP를 0. 10. 50과 100 ${\mu}M$의 농도로 첨가하여 아미노산 분석결과 PLP를 10${\mu}M$ 첨가하였을 때 10,424 $nM/{\mu}$l의 GABA가생산되었다. PLP를 첨가하지 않았을 때보다 PLP 첨가 후 GABA 생성이 증가됨을 확인할 수 있었다. For the screening of ${\gamma}-aminobutyric$ acid (CABA)-producing bacteria, 86 bacterial strains which produce GABA were isolated from Kimchi and Salted fisk .Among these, three strains designated AML15, AML45-1, AML72 with relatively high GABA productivity were selecled by thin layer chromatography (TLC). To elucidate the relationship between isolated strains and the genus Lactobacillus, their 16S rDNA sequence were examined. The result of their DNA sequences showed 99% similarity with Lactobacillus brevis ATCC 367. On the basis of the these results, isolated strains were identified as Lactobacillus brevis and designated L. brevis AML15. In order to determine the optimum conditions for GABA production, the isolated strains were cultivated in pyridoxal phosphate (PLP) and monosodium glutami. acid (MSG). Results showed that L. brevis AML15 had the highest CABA productivity with 10,424 $nM/{\mu}l$ concentration in MRS broth containing 5% (w/v) MSG and 10 ${\mu}M$ PLP at pH 5.0. The results imply that L. brevis AML15 has the potential to be developed as a strain for GABA hyper-production.

록각(鹿角)의 Adjuvant 관절염(關節炎)에 대한 실험적(實驗的) 연구(硏究)

신지원,박재영,박희수,Shin, Ji-Won,Park, Jai-Young,Park, Hee-Soo 대한약침학회 2002 Journal of pharmacopuncture Vol.5 No.1

Objective: To investigate effects of Cervi Cornu on Adjuvant Athritis in rats, the edema inhibit rate, the anaJgesic effects, the number of WBC, RA facter, Platelet, the quantity of CRP, total protein, albumin and globuline in the blood serum were measured in the arthritis part. Results: The results obtained as fonows ; 1. After arthritis of Sprague dawley(SD) rats was induced by injecting Freund's complete adjuvant for 2 weeks, any treatment was not for Control group, acupunctured for Treat Ⅰ group. normal saJine was ora] administrated for the Treat Ⅱ group, Cervi Cornu Ex. was oral administrated for Treat Ⅲ, and Cervi Cornu Herbal-acupullcture was injected for Trea Ⅳ group during 2 weeks every other day. Selected point was on pressure pain point in both groups. And then the edema inhibit rate were checked. The edema inhibit rate was $46.03\%$ in Treat I group, $43.24\%$ Treat IV group, $37.44\%$ in Treat III. there was significance in the edema inhibit rate between Control group and Treat group, in order of Treat Ⅰ, Ⅳ, Ⅲ.(p<0.05) 2. The analgesic effects was $7.58{\pm}1.80$(${\times}10$gm) in Control group. $11.00{\pm}1.10$(${\times}10$gm) in Treat Ⅰ group. $99.92{\pm}1.28$(${\times}10$gm) in Treat Ⅲ group and $14.67{\pm}1.03$(${\times}10$gm) in Treat Ⅳ group. There was significance in the analgesic effects between Control group and Treat group(p<0.05) 3. The number of WBC was $14.72{\pm}1.48$(${\times}10^3$㎕) in control Group, $10.26{\pm}1.13$(${\times}10^3$㎕) in Treat Ⅰ group, $11.00{\pm}1.13$(${\times}103$㎕) in Treat Ⅱ Group and $9.63{\pm}1.75$(${\times}10^3$㎕) in Treat Ⅳ group. There was significance in the number of WBC between Control group and Treat group(p<0.05) 4. The content of total protein in the blood serum were $6.13{\pm}0.05$g/dl in control group, $5.73{\pm}0.14$g/dl in Treat I group, $5.88{\pm}0.13$g/dl in Treat Ⅲ group and $5.90{\pm}0.13$g/dl in Treat IV group. There was significance in The content of total protein in the blood serum between Control group and Treat group(p<0.05) 5. The contests of albumin in the blood serum were $2.32{\pm}0.12$g/dl in the Control group, $2.35{\pm}0.05$g/dl in Treat Ⅰ group, $2.35{\pm}0.05$g/dl in Treat Ⅱ group, $2.30{\pm}0.06$g/dl in Treat Ⅲ group, $2.42{\pm}0.08$g/dl in Treat IV group. There was no significance in The content of albumin in the blood serum between Control group and Treat group(p<0.05) 6. The contests of globulin in the blood semm were $3.68{\pm}0.08$g/dl in the Control group, $3.43{\pm}0.12$g/dl in Treat Ⅰ group, $3.55{\pm}0.10$g/dl in Treat IV group. There was significance in The content of globulin in the blood serum between Control group and Treat group(p<0.05) 7. The numbers of RA factor were $3.47{\pm}0.54$IU/ml in Control group and $2.38{\pm}0.50$IU/ml in Treat Ⅱ group. There was significance inThe numbers of RA factor between Control group and Treat group(p<0.05) 8. The numbers of platelet were $1126.33{\pm}1126.33{\pm}85.93{times}10^3$/㎕ in Control group, $1043.33{\pm}80.80{times}10^3$/㎕ in Treat Ⅰ group, $1116.82{\pm}77.93{times}10^3$/㎕ in Treat Ⅱ group, $1164.17{\pm}94.02{times}10^3$/㎕l in Treat Ⅲ group, $1076.67{\pm}54.84{times}10^3$/㎕ in Treat Ⅳ group. There was no significance in The numbers of platelet between Control group and Treat group(p<0.05) 9. The quantity of CRP were $0.05{\pm}0.01$mg/ml in Control group, $0.05{\pm}0.01$mg/ml in Treat Ⅰ group, $0.06{\pm}0.01$mg/ml in Treat Ⅱ group, $0.05{\pm}0.00$mg/ml in Treat Ⅲ g

이상치에 근거한 선택적 실현변동성 예측 방법

신지원,신동완,Shin, Ji Won,Shin, Dong Wan 한국통계학회 2017 응용통계연구 Vol.30 No.3

실현변동성(RVs)이 지속적인 장기기억성과 상당히 큰 이상치의 존재로 인해 정상계열과 비정상계열의 경계에 위치한다는 것에 주목하였다. 실현변동성을 예측하기 위해 실현변동성 이상치 관측 유무에 따라 heterogeneous autoregressive (HAR) 모형과 integrated HAR (IHAR) 모형을 번갈아 사용하는 새로운 방법을 제안하였고, 이 방법을 IHAR-O-HAR라 칭하였다. 예측력 비교는 주요 지수인 S&P 500, Nasdaq과 Nikkei 225의 실현변동성 데이터를 이용하였으며 표본 외 예측력 비교에서 새로운 IHAR-O-HAR 방법은 RW 방법, HAR 방법이나 IHAR 방법의 예측력보다 우수함을 확인하였다. We note that the dynamics of realized volatilities (RVs) are near the boundary between stationarity and non-stationarity because RVs have persistent long-memory and are often subject to fairly large outlying values. To forecast realized volatility, we consider a new method that adaptively use models with and without unit root according to the abnormality of observed RV: heterogeneous autoregressive (HAR) model and the Integrated HAR (IHAR) model. The resulting method is called the IHAR-O-HAR method. In an out-of-sample forecast comparison for the realized volatility datasets of the 3 major indexes of the S&P 500, the NASDAQ, and the Nikkei 225, the new IHAR-O-HAR method is shown superior to the existing HAR and IHAR method.

Polyurethane과 Poly(Ethylene Oxide)를 이용한 hybrid 나노섬유 지지체의 제작

신지원,신호준,허수진,김지희,황영미,김동화,신정욱,Shin, Ji-Won,Shin, Ho-Jun,Heo, Su-Jin,Kim, Ji-Hee,Hwang, Young-Mi,Kim, Dong-Hwa,Shin, Jung-Woog 대한의용생체공학회 2006 의공학회지 Vol.27 No.5

The object of this study is to investigate the potential of dual-electrospun polymer based structure for vascular tissue engineering, especially for the medium or small sue blood vessels. Polyurethane(PU), which is known to be biocompatible in this area, was electrospun with poly(ethylene oxide) (PEO). Concentration of PU was fixed at 20wt%, while that of PEO was set from 15 to 35wt%. Morphological features were observed by SEM image and measurement of porosity and cellular responses were tested before and after extracting PEO from the hybrid scaffolds by immersing the scaffolds into distilled water. The diameter of PEO fibers were ranged from 200nm to 500nm. The lower concentration of PEO tended to show beads. The porosity of the scaffolds after extracting PEO was highly increased with higher concentration of PEO as expected. Also, higher proliferation rate of smooth muscle cells was observed at higher concentration of PEO than at the lower concentration and without PEO. As conclusions, this dual electrospinning technique combined with PU and PEO is expected to overcome the current barrier of cell penetration by providing more space for cells to proliferation.

물리적 인장 자극에 의한 줄기세포의 분화에 동반되는 미토콘드리아의 특성 변화에 관한 고찰

신지원 ( Ji Won Shin ),김혜림 ( Hye Lim Kim ),강윤경 ( Yun Gyeong Kang ),박소희 ( So Hee Park ),김영미 ( Young Mi Kim ),신정욱 ( Jung Woog Shin ) 한국조직공학과 재생의학회 2015 조직공학과 재생의학 Vol.12 No.1s

Recent studies have confirmed that the direction of stem cell differentiation can be modulated by physical stimuli even without biochemical growth factors. This implies stem cells attribute their differentiation to mechanical signaling directly or indirectly. However, the role or changes in mitochondria, one of the major energy sources in a cell, during differentiation have not been investigated. This study applied mechanical stretching of two different magnitudes (3% and 10%) to human mesenchymal stem cells without any relevant growth factors. We confirmed differentiation of stem cells into two different directions: osteogenic- and smooth muscle-like cells. Along with these results we found the expression of NOX4 was increased with stretching magnitude. As the expression of NOX4 is closely related to mitochondrial biogenesis and morphology various markers were also investigated. The expressions of the markers in relation to biogenesis (PGC-1alpha, TFA) and fusion (MFN1, MFN2, OPA1) were increased with stretching magnitude. However, the expression of Drp1 was observable with stretching of 3%. This study showed that the magnitude of physical stimulation affects the expression of NOX4, which has been known to be closely related to the biogenesis and morphological changes of mitochondria and stem cell differentiation. Therefore, further study of NOX4 due to physical stimulation for the control of stem cell differentiation accompanied by mitochondria functions is worth to be performed.

학동 전기 소아에서 알레르기 질환과 모유 수유와의 연관성

신지원 ( Ji Won Shin ),김우경 ( Woo Kyung Kim ),윤혜선 ( Hae Sun Yoon ) 대한소아알레르기호흡기학회(구 대한소아알레르기 및 호흡기학회) 2009 소아알레르기 및 호흡기학회지 Vol.19 No.4

목적: 알레르기 질환의 영유아시기 발병률이 높음에도 이 시기의 소아에 대한 조사가 미미한 상태로 이에 학동 전기 소아의 알레르기 질환 유병률을 알아보고자 하였으며, 국내 모유 수유정도와 기간을 조사하고 특히, 모유 수유와 알레르기 질환의 상관관계에 대해 알아보고자 하였다. 방법: 2004년 5-6월에 서울지역의 5개 유치원생 604명을 대상으로 하여 설문 조사를 실시하였다. 유병률은 `지난 12개월간 치료받은 적이 있었습니까?`를 기준으로 하였다. 각 설문의 항목에 대하여 부모나 보호자가 응답하도록 하였고, 이를 통 계 분석하였다. 결과: 알레르기 질환의 진단에 근거한 유병률은 각각 천식 4.5%, 알레르기비염 11.3%로 나타났고 아토피피부염은 19.0%이었다. 출생 후 첫 4개월간 모유만 수유한 경우는 21.8%였고 모유와 분유를 병행한 경우는 55%였으며 분유만 수유한 경우는 23.2%로 나타났다. 모유 수유 기간은 완전 모유 수유와 혼합 수유를 모두 포함하여 6개월 이하와 7개월 이상으로 분류하였고 각각 57.6%, 19.2%였다. 지난 12개월 동안의 천명을 묻는 질문에서는 모유를 7개월 이상 수유한 소아에서 유병률이 유의하게 낮았다. 아토피피부염에서는 모유 이외에 분유를 병행하였거나 분유를 먹인 경우 상대적 위험도가 높아졌다. 그러나 모유 수유 기간에 따른 통계학적 차이는 보이지 않았다. 결론: 서울지역 유치원생을 대상으로 알레르기 질환과 모유 수유와의 연관성을 알아보았다. 아토피피부염에서는 모유 이외에 분유를 병행하였거나, 분유만 먹인 경우 모유 수유보다 상대적 위험도가 의미 있게 높았으나 모유 수유 기간에 따른 차이는 없었으며 지난 l2개월 동안의 천명은 모유 수유를 오래 할수록 예방 효과를 보이는 것으로 나타났다. 학동전기 소아에서 부모에게 알레르기 병력이 있는 경우 천식 및 알레르기비염에서 위험이 높아진다. Purpose: The effect of breastfeeding on the development of allergic disease has been assessed in many studies but remains controversial. Thus, we evaluated the prevalence of allergic disease and its association with breastfeeding among preschool children. Methods: A questionnaire survey was conducted in 604 kindergarten children from 5 different kindergartens in Seoul between May and June, 2004. Parents or guardians were asked to answer the duration of breast-feeding and the time of weaning diet. Results: For the question, "Have you been treated for allergic disease during the past 12 months?" showed 4.5% of in asthma, 11.3% and 19.0% were in allergic rhinitis and atopic dermatitis, respectively. Exclusive breast-feeding for the first 4 months was noted in 21.8% of the children, compared with 55%, partial breast-feeding or only formula milk feeding in 23.2%. The prevalence of wheezing during the past 12 months was significantly lower in children with prolonged breast-feeding than in those with short-term breast feeding infants. There was a significant reduction in risk for atopic dermatitis in breastfeeding compared with formula milk feeding. Conclusion: Breastfeeding seems to have a preventive effect on the development of atopic dermatitis. The risk for wheezing, during the past 12 months was higher for in children with formula milk feeding. [Pediatr Allergy Respir Dis(Korea) 2009;19:374-382]

기능성 소화불량증 환자의 영양소 섭취 및 식행동

신지원 ( Ji Won Shin ),이금주 ( Geum Ju Lee ),박재우 ( Joe Woo Park ) 대한영양사협회 2012 대한영양사협회 학술지 Vol.18 No.4

As a disease that reduces quality of life, functional dyspepsia (FD) is associated with foods that may worsen its symptoms or cause it. The purpose of this study was to examine the nutritional status and dietary behaviors of FD patients. We investigated food intake, food intake frequency, and dietary habits of 45 FD subjects according to the Rome III Diagnosis Criteria. Average age and body mass index (BMI) were 47.7 years and 22.6 kg/m2 (males: 23.4 kg/m2, females: 22.1 kg/m2), respectively. Average energy intake was 77% of Korean Dietary Reference Intake (KDRI), and it was less than that of the Korea National Health and Nutrition Examination Surveys (KNHANES). Other nutrient intake levels were similar to the KNHANES. Energy intake proportion of carbohydrates:protein:fat was 56:18:26, and the ratio of fat intake was higher than that of the KNHANES. Beans, laver, tomato, and yogurt were consumed very frequently. In the results of meal regularity, dietary behaviors and composition of diet were relatively good. It is likely that the patient controlled their diet by eating cautiously and by reducing alcohol drinking and smoking. Our results indicate that FD patients` nutritional status by consumption of nutrients was at a level of normal healthy people and that dietary habits were better than normal adults. However, their fat intake levels were somewhat higher than normal people. Therefore, further research is required to identify the relationship between dietary intakes and FD.

노인의 SNS 활동을 통한 소통증진 프로그램에 대한 평가연구

신지원 ( Shin Ji Won ),권지성 ( Kwon Ji Sung ) 한국가족사회복지학회 2017 한국가족복지학 Vol.58 No.-

The purpose of this study was to evaluate the communication enhancement program through Social Network Service(SNS) of older adults in the community. This program was mainly designed for improving self-efficacy of older adults and enhancing their communication in the community by using SNS. This program was composed of several sub-programs; understanding the value of communication, meeting with SNS users, SNS education and practice, having activities with SNS, promoting SNS, holding public rehearsals and starting ceremony, and having off-line meeting. This study applied analytical framework based on logic model of systems theory, collected data from the subject group, and evaluated the sub-programs on dimensions of process and outcome. The results showed that this program has an effect on improving self-efficacy through participating SNS activities, enhancing communication through boosting SNS, creating new culture for older adults, and changing attitudes between generations. Based on these results, the practical guidelines for expanding the communication enhancement program through SNS, leading by older adults in the community, were suggested.

노인의 주도적인 SNS 활동을 통한 소통증진 프로그램에 대한 평가연구

신지원(Shin, Ji Won),권지성(Kwon, Jisung) 한국사회복지학회 2015 한국사회복지학회 학술대회 자료집 Vol.2015 No.4