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신정욱,박수아,김정락,신지원 대한의생명과학회 2006 Biomedical Science Letters Vol.12 No.2
In relation to the tissue engineering, the cellular responses to the morphology of the scaffold surface are interesting topics. Human ligament fibroblasts (HLFs) were cultured on the micrpatterned silicone substrates subjected to cyclic stretch to simulate ligament motion. Groove and ridge width of silicone substrates was 10/50, 20/50, 20/10, and 20/20 μm (groove/ridge μm) with a depth of 3 μm. Strain was applied over two days for 4 hours per day with a frequency of 0.5 Hz with the magnitudes of 4 or 8%. The purpose of this study was to evaluate ligament fibroblast alignment and cellular responses in relation to the pattern of microgrooved surface and stretching magnitude. Ligament fibroblasts in the microgrooved surface were elongated and aligned parallel to the microgrooves under no stretch. Uniaxial cyclic stretch induced cellular activities and their orientation rise in cellular response and the cells showed alignment and elongation perpendicular to the direction of the stretch. Biochemical analyses showed that the best cellular response was found on the 20/50 μm under 8% stretch. The surface morphology and mechanical stretching were found to contribute to increase of proliferation, collagen production.
인공 혈관용 나노 섬유 지지체에 관한 연구 : 세포 부착 및 조직 형성
신정욱,신호준,이용재,김인애,이창훈,조은희,김은정 한국생체재료학회 2004 생체재료학회지 Vol.8 No.2
The purpose of this study is to examine the potential of the nanofiber scaffold as a substrate for forming an artificial vascular graft. Aligned and randomly aligned polylatic-co-glycolic acid (PLGA) and polyurethane(PU) nanofiber scaffolds fabricated by electrospinning were used as square sheets. Fibroblasts harvested from human foreskin were seeded and cultured over 1 week period on a nanofiber scaffold, followed by seeding of endothelial cells harvested from human vessel. Thereafter the new tissue was analyzed for DNA, collagen and stained for immunohistochemistry with von Willebrand factor. DNA and collagen analysis of the seeded scaffold demonstrated that the human fibroblasts and endothelial cells attached to the polymeric fibers had begun to spread out (day 7) and extracellular matrix was saturated after 2 week. The immunohistochemistry examination demonstrated the seeded endothelial cells formed a monolayer on the fibroblasts and no endothelial cell invasion or new formation of capillaries could be detected. Indeed, there's no significant difference between aligned and randomly aligned nanofiber scaffold in all analyses. These results demonstrated that nanofiber scaffold used in this study might be a promising base material for tissue-engineered blood vessels.
신정욱,정성훈,정운선 대한신경정신의학회 2009 PSYCHIATRY INVESTIGATION Vol.6 No.3
Objective: The Adolescent Dissociative Experience Scale (A-DES) is a screening measure for dissociative experience in adolescents. The present study aimed to investigate the reliability, validity and psychometric properties of the Korean version of the Adolescent Dissociative Experience Scale. Methods: The Korean version of the A-DES was administered to a normative group of 371 adolescents aged 12 to 18 years and a traumatized group of 33 adolescents aged 12 to 18 years with known trauma. Results: The internal consistency was excellent (Cronbach’s alpha=0.91) and the testretest correlation of the A-DES was high (r=0.99). Correlation between the A-DES and other measures of dissociation was moderate (r=0.48). There were no significant age differences in mean total A-DES scores for the normative sample, or for boys or girls separately. Nor were there any significant gender differences for any age group. The mean total score of the A-DES was significantly higher in the traumatized group than in the normative group. There was a statistically significant difference between adolescents with self-reported trauma and those without a trauma history in the normative group. Conclusion: This study demonstrated that the Korean version of the A-DES is a reliable measure with excellent internal consistency and good stability over a 4-week test-retest interval with single factor structure. It can be used to screen for dissociative symptoms in Korean adolescents between the ages 12 and 18. Objective: The Adolescent Dissociative Experience Scale (A-DES) is a screening measure for dissociative experience in adolescents. The present study aimed to investigate the reliability, validity and psychometric properties of the Korean version of the Adolescent Dissociative Experience Scale. Methods: The Korean version of the A-DES was administered to a normative group of 371 adolescents aged 12 to 18 years and a traumatized group of 33 adolescents aged 12 to 18 years with known trauma. Results: The internal consistency was excellent (Cronbach’s alpha=0.91) and the testretest correlation of the A-DES was high (r=0.99). Correlation between the A-DES and other measures of dissociation was moderate (r=0.48). There were no significant age differences in mean total A-DES scores for the normative sample, or for boys or girls separately. Nor were there any significant gender differences for any age group. The mean total score of the A-DES was significantly higher in the traumatized group than in the normative group. There was a statistically significant difference between adolescents with self-reported trauma and those without a trauma history in the normative group. Conclusion: This study demonstrated that the Korean version of the A-DES is a reliable measure with excellent internal consistency and good stability over a 4-week test-retest interval with single factor structure. It can be used to screen for dissociative symptoms in Korean adolescents between the ages 12 and 18.