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화침요법(火針療法)에 관(關)한 문헌적(文獻的) 고찰(考察)
신용승,우수진,임수일,임재용,김종훈,김덕호,Shin, Young-seung,Woo, Su-jin,Lim, Su-il,Lim, Jae-yong,Kim, Chong-hoon,Kim, Dok-ho 대한침구의학회 2003 대한침구의학회지 Vol.20 No.4
Burning acupuncture is one of the methods of curing and preventing many kinds of diseases by inserting and taking out of the needle into particular points of the body so called acupuncture points, using a heated Dae-Chim which is one of the nine kinds of needles used in ancient times or by inserting a thick needle after its been heated to certain temperature. Nowadays in China, Burning Acupuncture is used thoroughly from musculoskeletal diseases to, internal medicine, gynecology and many other clinical departments. But in Korea, this method of cure is practiced by only a few clinicians, therefore by studying historical documents, our object is to recognize the importance of the Burning acupuncture and to offer the basis for the scientific researches and clinical application.
Isoeugenol의 넙치(Paralichthys olivaceus)에 대한 진정, 마취 및 독성효과 검토
신기욱,신용승,김영림,이은영,양향희,팔락샤,허남응,연성찬,이희천,오명주,조성준,강승원,정태성,Shin, Gee-wook,Shin, Yong-seung,Kim, Young-rim,Lee, Eun-young,Yang, Hyang-hee,Palaksha, K.J.,Huh, Nam-eung,Yeon, Seong-chan,Lee, Hee-chun,Oh, Myung- 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.2
Olive flounder (Paralichthys olivaceus) is the major mariculture fish in Korea. The annual aquaculture production of olive flounder in Korea during the period of 2003 was 300,000 ton (2002 Statistics, Ministry of Maritime Affairs & Fisheries, Korea Government). Anesthetics is very necessary in aquaculture to minimize stress and damage during harvesting, grading, transportation, spawning induction and handling to fish. In the present study, isoeugenol as new anesthetic in marine fish, especially olive flounder, was examined to know the efficacy and proper concentration. As a result, olive flounder adult was exhibited sedation at 5 ppm at 10 and $15^{\circ}C$, and 7.5 ppm at $20^{\circ}C$, respectively. Anesthesia was required at least 10, 7.5 and 10 ppm at $10^{\circ}C$, $15^{\circ}C$ and at $20^{\circ}C$, respectively. In case of fry, the effect of sedation was observed from 2.5 ppm at $10^{\circ}C$ and 5 ppm at 15 and $20^{\circ}C$, respectively. Anesthesia was observed from 2.5 ppm at $10^{\circ}C$, 5 ppm at 15 and $20^{\circ}C$, respectively. In acute toxicity test, it was impossible to explore $LD_{50}$ with the concentration of isoeugenol adult at $15^{\circ}C$ used, but over immersion volume of 15 ppm at $15^{\circ}C$ was observed mortality in fry. Based on the present study, isoeugenol was identified as a safe and active anesthetic to olive flounder.
이차원전기영동을 이용한 Escherichia coli O157:H7 균주간 항원 Spot의 비교
안영창,신기욱,신용승,이응구,이형준,박미림,김영림,정태성,김곤섭,김용환,Ahn, Yeong-Chang,Shin, Gee-Wook,Shin, Yong-Seung,Lee, Eung-Goo,Lee, Hyoung-Jun,Park, Mi-Rim,Kim, Young-Rim,Jung, Tae-Sung,Kim, Gon-Sup,Kim, Yong-Hwan 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.2
Proteomics is an emerging powerful tool in studying protein expression and function. At present study, proteomics was employed to evaluate the antigenicity among Escherichia coli O157:H7 strains using 2-dimensional gel electrophoresis (2-DE) and immunoblotting, SDS-PAGE and immunblotting analysis revealed no big differences among E coli O157:H7 strains. 2-DE analysis, however, revealed common antigens as well as specific antigens. The immunoblotting analysis revealed 20 common antigenic spots among E coli O157:H7 strains. In addition, there were 3 and 13 spots as common antigens between ATCC 43894 and KSC 109, and between ATCC 43894 and ACH 5, respectively. Antigenic spots specific for individual strain were also identified as 15, 8 and 22 for ATCC 43894, ACH 5 and KSC 109, respectively. The common antigens would be useful by employing either vaccine development or diagnosis marker, or both, whereas the specific antigens of individual strains would be applicable for epidemiological study. This study suggest that proteome analysis, representative as 2-DE, is valuable tool in exploring the E. coli antigenicity.