http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
손은숙,이영은,경선영,김세희,박정웅,김유진,정성환 대한결핵 및 호흡기학회 2018 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.126 No.0
Ambient airborne particulate matter(PM), an important component of environmental pollution, had been associated with multiple respiratory disease. Sulforaphane(SF) and Curcumin(CU) has a known as an antioxidant and anti-inflammatory agent in traditional medicine. We hypothesized that the combined SF/CU has protected against PM-induced lung damage by strong inhibition of oxidative stress and inflammation than single compound treatment. The results showed that PM10 exposure of epithelial cells increased ROS generation and pro-inflammatory cytokines. While, the treatment of SF, CU or combined SF/CU significantly increased the expression of Nrf2-downstream genes such as HO-1, NQO1-1, CAT and TXNRD1 by triggering the activate of Nrf2 transcription in the nucleus and decreased intracellular ROS generation. In addition, these compound strikingly inhibited the production of pro-inflammatory cytokines by downregulation the PM-induced inflammatory mediators, such as IL-6, IL-8, CXCL1 and MCP-1. The combined SF/CU showed the highest effectiveness in PM-induced epithelial injury. We confirmed these compound decreased the NF-κB signaling activation in PM-exposed epithelial cells. These results suggest that the combined SF/CU inhibited PM-induced oxidative stress and inflammation by increasing of antioxidant genes through Nrf2 transcriptional activity and by decreasing of pro-inflammatory cytokines through the mediation of NF-κB signaling. Our findings may provide experimental evidence and explanation for aggravated respiratory diseases by PM.
초고진공 Scanning Tunneling Microscope의 제작
손은숙,홍영규,박찬,Son, Eun-Sook,Hong, Yeong-Kyu,Park, Chan 한국진공학회 1994 Applied Science and Convergence Technology Vol.3 No.4
초고진공(UHV) Scanning Tummeling Microscopy(STM)을 제작하였다. 8인치 프란지에 부착한 STM은 초고진공에서 시료의 통전가열이 가능하며 다른 표면 측정방법의 적용과시료처리가 용이하다. 외부로부터 초고진공을 깨지 않고 시료와 tip의 도입이 가능하며 tip을 가열할 수 있다. 완성된 장치로 Si(111)-7$\times$7 구조의 STM상을 얻었다.
박정웅,손은숙,정성환,김세희 대한결핵 및 호흡기학회 2017 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.124 No.-
Background & Aims: Epithelial-mesenchymal transition (EMT), a process by which epithelial cells undergo a phenotypic conversion that gives rise to myofibroblasts, plays a pivotal process in the development of pulmonary fibrosis. From recent studies that hypoxia induces EMT of alveolar epithelial cells, and histone deacetylases (HDACs) are aberrantly overexpressed in basal cells of idiopathic pulmonary fibrosis (IPF) lungs, we aimed to elucidate whether hypoxia promotes mesenchymal transition of alveolar epithelial cell through activating HDACs. Methods: To evaluate the effect of HDACs in alveolar EMT, we performed quantitative real-time PCR, Western blot analysis, immunocytochemistry using siRNA transfection in human and rat alveolar epithelial cells, and immunohistochemistry using a bleomycin-induced fibrosis mouse model. Results: Hypoxia induced expression of alveolar EMT markers in A549 and RLE-6TN cells. Specifically, exposure to hypoxia decreased an epithelial marker, E-cadherin, and reversely increased expression of mesenchymal markers, Fibronectin and α -SMA in both cell lines. Additionally, a HDAC inhibitor, TSA was able to repress hypoxic effect such as loss of E-cadherin and gain of Fibronectin in alveolar epithelial cell. Among HDAC family, inhibition of HDAC3 using siRNA increased E-cadherin, and decreased Fibronectin, Snail, α-SMA in hypoxic conditions, and this process is dependent on Akt pathway. Conclusions: Histone deacetylase 3 induces mesenchymal transition of alveolar epithelial cell via repressing Akt via repressing Akt pathway in hypoxic conditions.
버섯의 분비단백질과 Laccase 유전자 발현에 있어서 사과박 추출물의 처리 효과
윤대은,손은숙,권오철,한우리자랑,박영진,이창수 한국버섯학회 2011 버섯 Vol.15 No.2
리그닌은 페닐프로판을 기본단위로 하여 구성된 방향족 거대분자로서 탄소-탄소 그리고 에테르 결합형으로 연결되어 있는 고분자 화합물이며 3차원적인 그물모양의 구조로 일정한 규칙성이 없고 난분해성이다. 리그닌 분해대사에 관여하는 미생물 중 특히 담자균류 중 백색부후균이 리그닌 분해력이 뛰어난 것으로 알려져 있다. 백색부후균은 리그닌은 잘 분해하지만 그 분해정도는 공기 중의 산소와 질소 농도, 보조기질의 첨가 그리고 배지조성의 성분 및 pH 등이 리그닌 분해에 크게 영향을 미친다고 알려져 있다. Laccase는 리그닌 분해효소 중 하나로서 대부분 균류와 고등식물에서 발견되며 다양한 페놀 화합물에 대해 반응을 하기 때문에 laccase mediator system의 잠재력에 관한 연구가 집중되고 있다. 사과박에는 이러한 페놀 화합물이 다량으로 함유되고 있는 것으로 보고되고 있다. 본 연구에서는 사과박 추출물 처리에 의한 버섯 균사체의 laccase 유전자 발현변화와 단백질 성분변화를 확인하고자 하였다. RT-PCR을 통해 유전자 발현의 분석 결과 일반배지에 비해 2배 정도 높았다. 또한 1DE와 2DE로 분비단백질을 분석한 결과 사과박 추출물의 첨가 농도에 의존적으로 단백질의 양이 증가함을 확인할 수 있었으며, 2DE를 통하여 사과박 첨가 유무에 따라 분비단백질의 발현 양상이 상이함을 확인하였다. 결과적으로 이 실험을 통해 사과박 추출물은 버섯 균사체의 laccase 유전자 발현을 증가시키며 분비단백질의 발현 양상에 큰 변화를 주는 것으로 확인되었다.
합리적 농촌지역정책 추진을 위한 지역선정방법 개선에 관한 연구
송두범,손은숙,김남선 한국국제지역사회개발학회 1998 地域社會開發學術誌 Vol.8 No.1
The objective of this study is by analyzing villages("Ri" units) to specify the proper unit of the area that the rural development policy is suitable for, and to examine whether the current rural development policy considers the characteristics of community and region. The areas of this study included twelve districts (in Korean "Eup" or "Myun") and one hundred and one villages(in Korean ri) in Poryong-si, Chungchungnam-do. Twelve and fifteen variables are respectively employed for the analysis of Myun's and Ri's. Using Factor Analysis, Cluster Analysis, and Z-Score Analysis, the study examines the degree of disadvantage and the process of growth pattern of each Myun or Ri. The Ri's are also classified according to their functional characteristics. The results of this study could be summarized as follows; 1) There exist some problems in the current rural development policy because it does not take into consideration the characteristics of community and region. 2) Except few distinct of areas, the proper unit of the area for the rural development policy should be set being based on regional characteristics rather than the administrative units. 3) The spatial boundary of rural development policy should be sets from villages(ri) to beyond the unit of administrative units("Si" or "Gun"), according to the village unit analysis. 4) It is needed that community and region data should be consistently accumulated after specifying Standard Statistical Districts. 5) The application of indicators should be in accordance with the characteristics of the policy.
P-123 Particulate matter deteriorates CSE-induced inflammation in bronchial epithelial cells
경선영,손은숙,홍정희,박정웅,정성환 대한결핵 및 호흡기학회 2017 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.124 No.-
Particulate matter (PM) is air pollution that induces adverse health effects in several organs, including the lungs. Epidemiologic evidence has shown an association between the concentration of PM and exacerbation of pulmonary diseases. We hypothesized that co-exposure to PM and cigarette smoke extract (CSE) may exacerbate the cellular toxicity and inflammation of bronchial epithelial cells. Human bronchial epithelial cells (BEAS-2B cells) cultured with or without 1-5% CSE for 7 days were exposed with or without each concentration of PM for 1 day. Inflammatory cytokines and mitogen-activated protein kinase (MAPK) pathways were evaluated by enzyme-linked immunosorbent assay (ELISA), reverse transcription-polymerase chain reaction (RT-PCR) or Western blot assay in the control, CSE, and CSE/PM groups. The inflammatory cytokines interleukin (IL)-6, IL-8, monocyte chemoattractant protein-1, chemokine (C-X-C motif) ligand 1, cyclooxygenase-2 and inducible nitric oxide synthase showed increased levels in CSE/PM-exposed cells compared with those in control or CSE-exposed cells. MAPK pathway evaluation via Western blotting revealed that ERK and P38 showed increased phosphorylation in CSE/PM-exposed cells. The inflammatory upregulation induced by CSE/PM exposure was attenuated significantly when cells were pre-treated with chemical inhibitors of ERK or p38.These results suggest that PM exacerbates inflammation in CSE-exposed bronchial epithelial cells through MAPK activation, such as ERK and p38 phosphorylation, leading to the production of inflammatory cytokines.