생쥐 초기 2-세포 배의 세포내 칼슘 증가에 미치는 Acetylcholine의 영향

윤숙영,강다원,배인하 韓國受精卵移植學會 2005 한국동물생명공학회지 Vol.20 No.3

체외 배양 과정 중에 나타나는 생쥐 초기 2-세포 배의 "in vitro 2-cell block" 현상은 세포내 농도 변화와 밀접한 관련이 있다. 다양한 종류의 세포에서 acetylcholine은 세포막에 존재하는 muscarnic acetylcholine receptor를 통해 세포내 농도 증가를 유도한다. 본 실험에서는 생쥐 "in vitro 2-cell block" 현상에 있어서 ACh의 영향을 알아보기 위해 세포 내 농도 조절 물질을 처리한 후 Many studies have shown that the development of mouse early 2-cell embryos in vitro is related with the intracellular changes. In ICR strain mouse, the development of embryos arrests at early 2-cell stage, but the arrested early 2-cell embryos can be rescued by the addition of -related materials. Acetylcholine (ACh) increases intracellular concentration ([]i) via the mAChR-PLC-IP3 pathway in mouse oocytes. We examined whether ACh rescues 2-cell block in mouse. In early 2-cell embryos, ACh increased []i in a dose-dependent manner (p<0.001), and had an effect on rescue of 2-cell block and embryonic development. To identify the signal pathway involved in ACh-induced rescue of 2-cell block, we first applied an agonist of ACh receptor (AChR). Like ACh, carbachol increased intracellular concentration ([]i) and atropine, an antagonist of ACh receptor, blocked the ACh-induced increase. In -free medium, ACh also increased []i, indicating that increased by ACh is mainly released from the intracellular store. The ACh-induced increase was blocked by PLC inhibitor (U73122), ryanodine receptor (RyR) antagonist (dantrolene), and CaM KII inhibitor (KN-93), but not by IP3R antagonists (xestospongin C). These results show that ACh increases intracellular concentration via mAChR/PLC/RyR, and this contributes to the rescue of 2-cell block.

Acute Hypoxia Activates an ENaC-like Channel in Rat Pheochromocytoma (PC12) Cells

배연주,유재철,박남미,강다원,황은미,박재용,홍성근,한재희 대한약리학회 2013 The Korean Journal of Physiology & Pharmacology Vol.17 No.1

Cells can resist and even recover from stress induced by acute hypoxia, whereas chronic hypoxia often leads to irreversible damage and eventually death. Although little is known about the response(s)to acute hypoxia in neuronal cells, alterations in ion channel activity could be preferential. This study aimed to elucidate which channel type is involved in the response to acute hypoxia in rat pheochromocytomal (PC12) cells as a neuronal cell model. Using perfusing solution saturated with 95%N2 and 5% CO2, induction of cell hypoxia was confirmed based on increased intracellular Ca2+ with diminished oxygen content in the perfusate. During acute hypoxia, one channel type with a conductance of about 30 pS (2.5 pA at╶80 mV) was activated within the first 2~3 min following onset of hypoxia and was long-lived for more than 300 ms with high open probability (Po, up to 0.8). This channel was permeable to Na+ ions, but not to K+, Ca+, and Cl╶ ions, and was sensitively blocked by amiloride (200 nM). These characteristics and behaviors were quite similar to those of epithelial sodium channel (ENaC). RT-PCR and Western blot analyses confirmed that ENaC channel was endogenously expressed in PC12 cells. Taken together, a 30-pS ENaC-like channel was activated in response to acute hypoxia in PC12 cells. This is the first evidence of an acute hypoxia-activated Na+ channel that can contribute to depolarization of the cell.

사용자의 시선과 팔 움직임 특징 정보를 이용한 픽앤플레이스 움직임 의도 추정

주다원(Dawon Ju),배준범(Joonbum Bae) 대한전자공학회 2023 대한전자공학회 학술대회 Vol.2023 No.11

Teleoperated robot perform tasks instead of human operator due to physical limitations or places difficult to go. However, because of kinematic inconsistencies between operator and teleoperated robot, the operator has to physical fatigue and affects the operator performance. To overcome this limitation, one solution is to predict the intention of the human operator through operator’s behavior and assist operator before the end of the work. Therefore, in this paper, we propose and LST-Mbased algorithm to predict operator’s Pick-and-Place intention using gaze and arm movement. We train the proposed model using sequential data and learn LSTM based model and real-time motion recognition result is shown.

Control of Ca^(2+)- Influx by Ca^(2+)/Calmodulin Dependent Protein Kinase II in the Activation of Mouse Eggs

윤숙영,강다원,배인하 한국발생생물학회 2011 발생과 생식 Vol.15 No.1

Change in intracellular Ca^(2+)-concentration ([Ca^(2+)]i) is an essential event for egg activation and further development. Ca^(2+) ion is originated from intracellular Ca^(2+)-store via inositol 1,4,5-triphosphate receptor and/or Ca^(2+) influx via Ca^(2+) channel. This study was performed to investigate whether changes in Ca^(2+)/calmodulin dependent protein kinase II (CaM KII) activity affect Ca^(2+) influx during artificial egg activation with ethanol using Ca^(2+) monitoring system and whole-cell patch clamp technique. Under Ca^(2+) ion-omitted condition, Ca^(2+)- oscillation was stopped within 30 min post microinjection of porcine sperm factor, and ethanol-induced Ca^(2+) increase was reduced. To investigate the role of CaM KII known as an integrator of Ca^(2+)- oscillation during mammalian egg fertilization, CaM KII activity was tested with a specific inhibitor KN-93. In the eggs treated with KN-93, ethanol failed to induce egg activation. In addition, KN-93 inhibited inward Ca^(2+) current (I_(ca)) in a time-dependent manner in whole-cell configuration. Immunostaining data showed that the voltage-dependent Ca^(2+) channels were distributed along the plasma membrane of mouse egg and 2-cell embryo. From these results, we suggest that Ca^(2+) influx during fertilization might be controlled by CaM KII activity.

Internal Perfusion of ADP in Mouse Oocytes Increases Outward Currents

한재희,박홍기,강다원,이상미,이상호,배인하,홍성근 한국발생생물학회 2000 발생과 생식 Vol.4 No.2

To find the mechanism underlying the ADP-induced increase in the outward current in ovulated mouse oocytes, we examined changes in voltage-dependent currents using the whole cell voltage clamp technique and the internal perfusion technique. Eggs were collected from the oviduct of superovulated mice with PMSG and hCG. Membrane potential was held at -60 mV (or -80 mV in the case of recording currents) and step depolarizations or hyperpolarizations were applied for 300 ms. By step depolarizations, outward currents comprising steady-state and time-dependent components were elicited. They were generated in response to the positive potential more than 20 mV with severe outward rectification and were blocked by external TEA, a specific channel blocker, suggesting that they be carried via channels. Internally-perused 5 mM ADP gradually increased outward currents (IK) 1 min after perfusion of ADP and reached slowly to maximum (150~170%) 5 min later over the positive potential range, implying that ADP might not be acted directly to the channels. IK were decreased by 5 mM ATP without affecting the steady-state component of outward current. In contrast to the effect of ADP and ATP on IK, both effect of ATP and ADP on inward currents (ICa) could not be detected due to the continuous decrease in current amplitudes with time-lapse ("run-down" phenomena). To check if there is a G protein-involved regulation in the ionic current of mouse oocytes, 1 mM GTP was applied to the cytoplasmic side, and the outward current and inward currents were recorded. ICa was promptly increased in the presence of GTP whereas IK was not changed. from these results, it is concluded that the ATP-dependent regulation is likely linked in the ADP-induced increase in the outward current, and G protein-involved cellular signalling might affect ion channels carrying and in mouse oocytes.

한국과 일본의 노인돌봄 인력 분석: 간병인과 방문개호원 중심으로

김두리,배다연,배다원 한국산학기술학회 2023 한국산학기술학회논문지 Vol.24 No.11

This study compared and analyzed caregivers who provide long-term care to the elderly in Korea, and visiting caregivers in Japan, focusing on the definition, qualification process, education process, availability of insurance services, manpower supply, and treatment. The study revealed that caregivers in South Korea need to obtain a "caregiver" certificate, which is a civilian qualification, and visiting caregivers in Japan are eligible for visiting care when they complete the training for first-time caregivers. Multiple-choice certification exams were to be given in Korea, and multiple-choice, subjective, and oral tests were recommended in Japan. The passing standard was 60 out of 100 in Korea and 70 in Japan. While there was no stipulation that caregivers in South Korea had to complete a fixed standard of training hours, in Japan, at least 130 hours of training were required to provide visiting care. Regarding insurance services, it was difficult for Koreans to receive support for nursing expenses in practice, but in Japan, it was possible to receive visiting care support if the long-term care rating was recognized through the long-term care insurance system. The average age of caregivers was 50~70, visiting caregivers were 40~60 years old, and the average wage level was 2.4 million won per month for caregivers and 2.59 million won per month for visiting caregivers as of 2020. As of 2017, the proportion of non-regular workers was 61.6% for caregivers and 69.7% for visiting caregivers. Based on the results of this study, it is believed that it would be necessary to develop a systematic qualification process and curriculum for caregivers in the future.

A practical synthetic process for a analgesic drug

서영란,서상원,김중원,채다원,김영규,황진호,배성수 한국공업화학회 2013 한국공업화학회 연구논문 초록집 Vol.2013 No.-

간호대학생의 치매 노인에 대한 태도에 영향을 미치는 요인

김두리,박지연,박지연,방지윤,배다연,배다원,진혜진 한국산학기술학회 2024 한국산학기술학회논문지 Vol.25 No.3

우리나라가 2025년 초고령사회로 진입하면서 치매노인에 대한 간호 수요 증가가 예상되며 직접적인 간호를 제공하는 간호사의 치매노인에 대한 긍정적인 태도는 간호의 질과 직결된다. 이에 본 연구는 추후 치매노인에게 간호 수행을제공할 기회가 많은 간호대학생을 대상으로 치매 노인의 태도에 영향을 미치는 요인을 알아보기 위해 시도되었다. 본연구는 서술적 조사연구로 자료수집을 위해 D시 일개 간호대 150명을 대상자로 하여 구조화된 설문을 통해 자료를 수집하였고, SPSS for Win 21.0을 통해 분석하였다. 본 연구 결과 치매 노인의 태도는 노인접촉경험(r=.239, p=.003), 공감능력과 정적 상관관계(r=.273, p<.001)가 있는 것으로 나타났으며, 성별(β=.23, p=.004), 노인관련 봉사활동 경험(β =-.206, p=.009), 공감능력(β=.181, p=.021), 노인접촉경험(β=.167, p=.034)이 치매 노인의 태도에 영향을 미치는 요인으로 분석되었다. 또한 이 변수들의 치매노인 태도에 대한 설명력은 19.0% 이었다. 본 연구는 연구 결과를 토대로치매노인에 대한 간호대학생의 간호역량 함양을 위하여 영향요인들을 증진시킬 수 있는 교육 프로그램 개발의 기초자료로 활용될 수 있다는 것에 의의가 있다.