치매노인의 인지능력에 미치는 꽃장식의 효과와 꽃의 선호도 조사

윤숙영,김홍열,--,우태양 한국화훼연구회 2002 화훼연구 Vol.10 No.1

본 연구는 대구광역시에 위치한 <햇빛치매어르신주간보호센터>에 있는 치매노인을 대상으로 꽃장식이 치매노인의 인지기능에 미치는 영향을 알아보고 꽃장식에서 꽃의 색과 크기의 선호도를 조사하기 위해서 치매노인 6명을 대상으로 2002년 2월 14일부터 2002년 5월 9일까지 주 1회씩 총 12회 원예치료 프로그램을 실시하였다. 대상자는 모두 여성이었다. 평가결과는 다음과 같다. MMSE-K 평가결과 대상자 A는 원예치료 실시 전 13점에서 실시 후 18점으로, 대상자 D는 원예치료 실시 전 5점에서 13점으로 유의하게 향상되는 것을 볼 수 있었다. 대상자 B는 7점에서 8점으로, 대상자 E는 3점에서 7점으로 향상되었으나 유의차가 없었다. 대상자 C는 원예치료 실시 전 15점에서 실시 후에는 14점으로 오히려 낮아졌으나 유의차는 없었다. 대상자 F는 인지능력이 없는 대상자로서 실시 전후 큰 변화가 없었다. 꽃의 선호도 조사결과 색은 적색과 황색 그리고 분홍색 순으로 선호하였고 크기는 중간형보다는 크거나 작지만 풍성한 꽃을 선호하였다. 이상의 결과로부터 꽃장식은 치매노인의 인지기능 향상에 효과적이었다. 재료는 매회 달랐지만 작품을 구사하여 다듬고, 자르고, 꽂고 하는 반복적인 활동을 통해 흥미를 유발하여 적극적으로 자신감을 갖게 되었으며, 또한 결과물을 가족들과 함께 공유하게 함으로써 가족관계도 개선되었다. Abstract. This study set out to research preference of flowers, to analyze the impacts of floral decoration on the improvement of recognition ability in the demented old adults in order to develop an more effective horticultural therapy program for the demented old adults. The subjects were made out of the demented old adults who were taken care of at <HABIT Demented Old Adults Day Care Center> located in Icheon-dong, Nam-gu, Daegu Metropolitan City. Total six subjects were included in experiment. They were all female, and their average age was 76. The experiment was conducted once a week from February 14 to May 9, 2002, total in 12 times. The experiment was only focused on floral decorations. The color and size of flowers were considered to decide the subjects preference. The subjects preferred red and yellow flowers. They also liked big flowers or small but opulent ones. As for floral decoration, the material flowers were different each session. But the subjects were aroused into interest via repetitive activities such as designing their works and arranging, cutting, and putting in flowers. Their active and confident attitudes became evident, and their relationships with their family grew better as they shared their works with them.

치매노인의 인지능력에 미치는 꽃잠식의 효과와 꽃의 선호도 조사

윤숙영,김홍열,우태양 한국화훼산업육성협회 2002 화훼연구 Vol.10 No.1

Role of Type 1 Inositol 1,4,5-triphosphate Receptors in Mammalian Oocytes

윤숙영 한국발생생물학회 2019 발생과 생식 Vol.23 No.1

The ability of oocytes to undergo normal fertilization and embryo development is acquired during oocyte maturation which is transition from the germinal vesicle stage (GV), germinal vesicle breakdown (GVBD) to metaphase of meiosis II (MII). Part of this process includes redistribution of inositol 1, 4, 5-triphosphate receptor (IP3R), a predominant Ca2+ channel on the endoplasmic reticulum membrane. Type 1 IP3R (IP3R1) is expressed in mouse oocytes dominantly. At GV stage, IP3R1 are arranged as a network throughout the cytoplasm with minute accumulation around the nucleus. At MII stage, IP3R1 diffuses to the entire cytoplasm in a more reticular manner, and obvious clusters of IP3R1 are observed at the cortex of the egg. This structural reorganization provides acquisition of [Ca2+]i oscillatory activity during fertilization. In this review, general properties of IP3R1 in somatic cells and mammalian oocyte are introduced.

초자화동결을 이용한 제 3일째 생쥐 배아의 동결보존

윤숙영,손철,배인하,Yoon, Sook-Young,Sohn, Cherl,Bae, In-Ha 대한생식의학회 1997 Clinical and Experimental Reproductive Medicine Vol.24 No.3

The use of hormonal stimulation in human in vitro fertilization and embryo transfer (IVF-ET) leads to increased production of embryos for ET. So to avoid high pregnancies and to allow conception in future, unstimulated cycles, cryopreservation of spare embryos is desirable. One of the improvement of cryopreservation methods is vitrification. We cryopreserved mouse day 3 embryos by vitrification using the three different vitrification solution (EFS40, VS11 and VS3a). EFS40 solution is consisted of 40% (v/v) ethylene glycol, Ficol170 30% (w/v) and 0.5M sucrose and VS11 is 6.0M ethylene glycol and 1.8M glycerol. And VS3a is 6.5M glycerol and 6% (w/v) BSA (bovine serum albumin). First we tested the toxicity of three vitrification solution by exposure to these solution during 3 min. After washing by thawing solution, the survival rates of each groups are 95.5%, 90.9% and 84.4% (EFS40, VS11 and VS3a). High percentages of them developed to expanded blastocyst and hatching embryos in culture 48hrs 94.2%, 97.7%, 100% and 97.4% (no treatment group, EFS40, VS11 and VS3a). So there is no significant differences among the each group. Second, after thawing of vitirfied embryos, the survival rates of each groups are 96.8% (slow freeze), 94.1% (EFS40), 85.5% (VS11) and 80.0% (VS3a, P vs. no freeze or EFS40 is 0.01). Vitrified embryos exhibited a high rate of development in vitro after 48hrs culture. The percentages of each group to blastocyst and hatching embryos are 88.7% (no freeze), 91.8% (slow freeze), 93.4% (EFS40), 87.7% (VS11) and 73.0% (VS3a, P vs. other group is 0.01). The results suggest that there is no significant differences in exposure of various vitrification solution and day 3 mouse embryos can be vitrified in solution EFS40 and VS11 by simple procedure.

설악산을 다녀와서

윤숙영 이화여자대학교 미술대학 자수학회 1974 繡 Vol.- No.2

Use of Environment-friendly Fixing Materials in Flower Arrangement

윤숙영,최병진 인간식물환경학회 2009 인간식물환경학회지 Vol.12 No.5

This study was purposed to propose environment-friendly flower arrangement methods by suggesting flower fixing materials those are excellent in fixing ability as a pin-holder or floral foam, harmless to the environment, and highly aesthetic. For this purpose, we analyzed the works presented in the florist training program of German GBF (Grunbergr Bildungszentrum Floristik) in August, 2008. In the analysis, fixing materials were grouped into materials obtainable from nature, and recycled ones. When flowers were arranged using plant organs, namely, leaf, stem, root, and berry as flower fixing materials, they were good as floral foam in fixing ability, and were excellent in erecting flowers. In addition, recycled materials were not good as natural objects in fixing ability, but showed possibilities in fixing and erecting as long as flowers are selected fittingly for the empty space and bearing force of such materials. As presented above, natural objects including plants and recycled products used as environment-friendly flower fixing materials showed fixing and erecting abilities as good as a pin-holder or floral foam. Accordingly, it is considered desirable to restrain the use of floral foam as much as possible and to arrange flowers with environment-friendly materials. This study was purposed to propose environment-friendly flower arrangement methods by suggesting flower fixing materials those are excellent in fixing ability as a pin-holder or floral foam, harmless to the environment, and highly aesthetic. For this purpose, we analyzed the works presented in the florist training program of German GBF (Grunbergr Bildungszentrum Floristik) in August, 2008. In the analysis, fixing materials were grouped into materials obtainable from nature, and recycled ones. When flowers were arranged using plant organs, namely, leaf, stem, root, and berry as flower fixing materials, they were good as floral foam in fixing ability, and were excellent in erecting flowers. In addition, recycled materials were not good as natural objects in fixing ability, but showed possibilities in fixing and erecting as long as flowers are selected fittingly for the empty space and bearing force of such materials. As presented above, natural objects including plants and recycled products used as environment-friendly flower fixing materials showed fixing and erecting abilities as good as a pin-holder or floral foam. Accordingly, it is considered desirable to restrain the use of floral foam as much as possible and to arrange flowers with environment-friendly materials.

Forest Activities for Developing Emotional Vocabulary and Lowering Cortisol Levels in Kindergarteners

윤숙영,설가애,장현희,김미진,최병진 인간식물환경학회 2019 인간식물환경학회지 Vol.22 No.4

The purpose of this study was to investigate the effects of forest activities on the cortisol concentrations in the body and emotional vocabulary of kindergarteners. The participants were 36 kindergarten children aged 4-5, 17 in the control group and 19 in the experimental group. Forest activities consisted of six steps according to the level of kindergarteners, and the program was carried out at G City Environment Training Institute. Before and after the experiment, the children’s emotional vocabulary was self-reported and salivary cortisol was analyzed to evaluate changes in the body 's stress hormones. As a result, in the control group, positive words increased significantly from 0.14 to 1.53 (p=.003), but the decrease in negative words was not significant (p=.059). In the experimental group, positive words increased significantly from 0.47 to 2.84 after forest activities (p=.000). Negative words decreased significantly from 1.94 to 0.00 after forest activities (p=.000). In the analysis of salivary cortisol, the change in salivary cortisol concentrations was not significant (p=.667) in the control group, merely decreasing from 0.179 μg/dl to 0.161 μg/dl. However, the experimental group showed a significant decrease from 0.202 μg/dl to 0.161 μg/dl after forest activities (p=.049). These results suggest that forest play activities may be helpful in encouraging young children‘s emotional development by increasing positive vocabulary and decreasing negative vocabulary and reducing stress in kindergarteners.

생쥐 초기 2-세포 배에서 세포 내 칼슘 농도의 변화에 $Ni^{2+}$이 미치는 영향

윤숙영,이은미,배인하,Yoon, Sook-Young,Lee, Eun-Mi,Bae, In-Ha 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.4

Objective: We reported the overcoming effect of $Ni^{2+}$ on the in vitro 2-cell block of mouse embryos. In this study, we aim to investigate whether $Ni^{2+}$ should induce intracellular $Ca^{2+}$ transient in the mouse embryos. Materials and Methods: Embryos were collected at post hCG 32hr from the oviduct of the ICR mouse and cultured in M2 medium omitted phenol red. Intracellular $Ca^{2+}$ was checked by using a confocal laser scanning microscope and fluo-3AM by using various intracellular $Ca^{2+}$ antagonists. Results: In 1mM $Ni^{2+}$ treated medium which contained $Ca^{2+}$(1.71mM), 75.7% of the embryos showed $[Ca^{2+}]i$ transient about 200 sec later. In the $Ca^{2+}$-free medium, 69.8% of the embryos showed $[Ca^{2+}]i$ transient. In U73122, phospholipaseC(PLC) inhibitor (5uM, 10min) pretreated group, 33.3% of the embryos showed $[Ca^{2+}]i$ transient. Heparine, inositol 1, 4, 5-triphosphate receptor(IP3R) antagonist preinjected embryos showed no response with 1mM $Ni^{2+}$. In danthrolene treatment, ryanodine receptor(RyR)-antagonist, 43% embryos showed $[Ca^{2+}]i$ transient but they showed delayed response about 340sec in the presence of $Ca^{2+}$. Conclusions: Summing up the above results, $Ni^{2+}$ seems to induce $Ca^{2+}$-release from the $Ca^{2+}$-store even in the $Ca^{2+}$-free medium. IP3 receptors of the mouse 2-cell embryos might have an essential role for the intracellular $Ca^{2+}$ increase by $Ni^{2+}$.

원예치료가 치매노인의 일상생활 수행능력과 인지기능에 미치는 영향

윤숙영,김홍열 경상대학교 농업생명과학연구원 2009 농업생명과학연구 Vol.43 No.5

This study purposed to examine the effect of the horticultural therapy on activities of daily living and cognitive function of demented old adults in facilities. According to the results of evaluating BADL, the ability to perform daily activities decreased from 9.71 to 9.21 in the control group, but in the experimental group, the ability to perform daily activities increased significantly(p=.009) from 10.86 before the horticultural therapy to 12.43 after the therapy. According to the results of evaluating K-MMSE, the score decreased from 10.57 to 9.07 in the control group, but the experimental group showed a significant improvement in cognitive functions from 10.43 before the horticultural therapy to 13.29 after the therapy(p=.003). As presented above, the horticultural therapy was effective in improving activities of daily living and cognitive function of demented old adults. 본 연구는 원예치료가 치매노인의 일상생활 수행능력과 인지기능에 미치는 영향에 대해서 알아보고자 실시하였다. 연구방법은 시설에서 요양 중인 치매노인을 대상으로 정서적인 안정과 손 기능은 물론 인지기능을 강화하여 일상생활 수행능력을 향상시키고자 다양한 원예활동을 실시하였다. 원예치료 실시 전과 후의 일상생활 수행능력의 변화를 평가한 결과 대조군은 9.71점에서 9.21점으로 시간이 지날수록 일상생활 수행능력이 감소되었으나 실험군은 원예치료 실시 전 10.86점에서 실시 후 12.43점으로 일상생활 수행능력이 유의하게(p=.009) 향상되었다. 그리고 인지기능의 변화를 평가한 결과 대조군은 10.57점에서 9.07점으로 인지기능이 떨어졌으나 실험군은 원예치료 실시 전 10.43점에서 실시 후 13.29점으로 인지기능이 유의하게(p=0.003) 향상되었다. 이와 같이 치매노인에게 식물을 매개체로 하는 다양한 원예활동은 치매노인의 일상생활 수행능력뿐만 아니라 인지기능 향상에 효과적이었다

꽃장식이 입원노인의 감정균형과 우울에 미치는 영향

윤숙영,최병진,김홍열 인간식물환경학회 2009 인간식물환경학회지 Vol.12 No.6

This study was conducted in order to examine the effects of horticultural therapy using flower arrangement on the balance of feeling, depression, and self‐esteem in hospitalized elders who could not go out. For this purpose, we applied horticultural therapy to six elderly inpatients at a geriatric hospital in D City once a week and a total of 12 sessions from February 18 to May 6, 2009. The program consisted of flower arrangement only, and it stimulated the patients’ five senses using diverse forms, colors, and smells of flowers in order to give emotional stability. In addition to flower arrangement, the elderly patients had various play activities like sharing memories, signing songs, and reciting poems related to flowers. Before and after flower arrangement, the subjects’ emotional change was examined in terms of the balance of feeling, depression, and self‐esteem. According to the results of this study, the mean score of balance of feeling was 30.00 before flower arrangement but increased significantly to 35.50 after. The mean score of depression decreased significantly from 6.00 before flower arrangement to 3.33 after (p=0.025). The mean score of self‐esteem increased significantly from 14.00 before flower arrangement to 17.67 after (p=0.012). As demonstrated by these results, when horticultural therapy using flower arrangement was applied to elderly inpatients, it caused positive change in their balance of feeling, depression, and self‐esteem, and consequently was effective in their emotional stability. 본 연구는 밖으로 나가지 못 하는 입원한 노인들을 대상으로 꽃꽂이를 이용한 원예치료가 감정균형, 우울, 자아존중감에 어떠한 영향을 주는지를 알아보고자 실시하였다. 연구방법은 D시에 위치한 노인전문병원에 입원한 노인 6명을 대상으로 2009년 2월 18일부터 5월6일까지 주 1회씩 총 12회를 실시하였다. 프로그램은 꽃꽂이만을 하였으며 정서적 안정을 주기 위해 다양한 형태, 색, 향기 등의 꽃을 이용하여 오감을 자극하였다. 또한 추억의 소재를 통한 이야기 나누기, 꽃과 관련된 노래 부르기, 시 외우기 등 다양한 놀이 활동을 꽃꽂이와 함께 함으로써 즐거움을 제공하였다. 측정도구는 감정균형, 우울, 자아존중감의 항목으로 꽃장식실시 전과 후의 정서적인 변화를 살펴보았다. 평가결과는 먼저 감정균형에서는 꽃장식 실시 전 평균 30.00점이었으나 실시 후 평균 35.50점으로 유의하게 증가되었으며, 우울정도는 실시 전 평균 6.00점 이었으나 실수 후 평균이 3.33점으로 유의하게(p=0.025)감소되었다. 자아존중감 정도에서는 꽃장식 실시 전 평균 14.00점 이었으나 실시 후 평균 17.67점으로 유의 하게(p=0.012) 증가하였다. 이상의 연구결과와 같이 꽃장식을 이용한 원예치료를 실시한 결과 입원노인들의 감정균형, 우울, 자아존중감에 긍정적인 변화를 초래하여 정서적 안졍에 효과가 있었다.