Identification of Differentially Expressed Genes between Neonatal and Peripubertal Rat Thymi Using GeneFishingTM Polymerase Chain Reaction

강다원,Gyu-Tae Kim,한재희 사단법인 한국동물생명공학회 2007 Reproductive & developmental biology Vol.31 No.1

Aging causes thymus involution, and genes in thymus play an important role in the development of the immune system. In this study, we compared genes expressed in thymus of neonatal and peripubertal rats using annealing control primers (ACPs)-based GeneFishing polymerase chain reaction (PCR) and semiquantitative reverse transcription (RT)-PCR. We identified 10 differentially expressed genes (DEGs) with 20 ACPs. Of 10 DEGs, bystin-like, collagen type V alpha 1 (COL5A1), and T-cell receptor beta-chain segment 2 (TCRB2) that are related to immune-function were detected in rat thymus. Bystin-like and TCRB2 were up-regulated, while COL5A1 was down-regulated in peripubertal thymus. Semiquantitative RT-PCR confirmed postnatal changes in expression of bystin-like, COL5A1, and TCRB2. These results suggest that bystin-like, COL5A1, and TCRB2 could regulate immune function controlled in thymus as age increases.

외식산업의 정보기술이 공급자-구매자 관계 및공급사슬관리 성과에 미치는 영향에 관한 사례연구

강다원 한국외식경영학회 2006 외식경영연구 Vol.9 No.1

Today’s food-service business environment has become rapidly changed from the development of internet and information technology. This study empirically analyzed whether there exists a difference in the supplier-buyer relationship according to products and analyzed application of information technology in the procurement system of food-service companies.The results of case study can be summarized as follows: First, accordingly characteristics of products, the core products that is important strategically showed high-collaboration relationship. And general products that is unimportant strategically showed that existed a competition because of low-interdependence. Secondly, the present introduction condition of IT existed a difference according as characteristics of buyer that is business conditions. Group meals enterprise utilized ERP system and tried to conduct Supply Chain Management and most Family Restaurants also used ERP system. But most Korean Restaurants didn’t use internet-procurement. Finally, after that introduction of IT in food-service industry exists significant change that is clear in procurement-business connection.

제주방문 중국관광객의 외식성향 및 서비스 만족에 관한 연구

강다원 한국외식경영학회 2003 외식경영연구 Vol.6 No.1

The objectives of this research are to analyse Chinese tourist`s eating-out tendency and food-service satisfaction in Jeju where is designated `No-visa Area` for Chinese tourist and to suggest the food-service marketing strategies which is able to satisfy Chinese tourists and activate Chinese tourist`s visit. According to the finding, Chinese tourist tend to prefer Chinese food to Korean food during their stay in Korea. They also want the certain recipe including greasy food or sweetness. Among the Korean food. They like Sam-Ge-Tang, Galbi, Bibimbap. The results of this study suggest that there is a significant difference between educational background·region·occupation and food-service satisfaction. And as the result of regression analysis to examine what kind of variables influence eating-habits satisfaction. menu diversity·service quality·reception attitude·cleanliness level had a significant influence upon satisfaction.

에스트로겐이 생쥐 초기배의 $\textrm{Ca}^{2+}$ 전류에 미치는 영향

강다원,신용원,김은심,홍성근,한재희 사단법인 한국동물생명공학회 2001 한국동물생명공학회지 Vol.16 No.2

배분화과정시 나타나는 $Ca^{2+}$ 변화에 미치는 $E_2$의 영향을 알아보고자 whole cell voltage clamp 기법, 방사선 등위원소 면역측정법, 그리고 공초점 현미경을 통하여 $E_2$처리 후 나타나는 $Ca^{2+}$ 전류 변화 및 세포내 $Ca^{2+}$ 농도 변화를 조사하였다. 생쥐의 미성숙 난자는 난소의 난포를 천자하고, 배란난자는 과배란 처리 후 난관에서 회수하였다. 수정란은 과배란 처리 후 수컷 생쥐와 교미를 유도한 후 각각의 단계에 맞는 수정란을 채란하였다. 혈중 $E_2$의 농도는 심장을 천자하여 혈액을 채취한 후 배발달 단계와 호르몬 처리 시간이 일치하는 혈액만을 사용하였다. 본 실험의 결과를 요약하면 다음과 같다. 1. $E_2$처리시 미성숙난자의 제 1극체 형성률 (성숙의 지표)은 $E_2$를 처리하지 않은 난자(83% : 83/100)보다 $E_2$를 처리한 난자 (94%, 94/100)에서 유의적 (P<0.05)으로 높게 나타났다. 2. $E_2$를 처리하였을 때 $Ca^{2+}$ 내향전류의 변화는 -10 mV에서 -1.23$\pm$0.01 nA (n=15)에서 -1.50$\pm$0.03 nA (n=15)로 122% 상승함으로써 유의한 (P<0.05) 변화를 보였다. 3. $E_2$를 처리하지 않은 난자 및 수정란을 1로 한 후 $E_2$를 처리한 난자 및 수정란의 변화를 상대적인 값으로 표시하였다. $E_2$처리한 난자는 1.22$\pm$0.17 (n=10), $E_2$처리한 전핵배는 1.20$\pm$0.14 (n=10), $E_2$처리한 2세포기배는 1.07$\pm$0.01 (n=10), 4세포기배는 1.05$\pm$0.09 (n=10)를 나타냄으로써 수정란의 단계마다 $E_2$의 반응 결과가 차이가 남을 알 수 있었다. 4. $E_2$농도 곡선에서 PMSG 처리 후 $E_2$의 혈중농도는 계속적인 상승을 보이다가 배란시기에 최고치를 나타내었으며, 배란 후 다시 감소하여 8세포기에서는 급격한 감소현상이 나타났다. 이후 다시 상실기를 거쳐 배반포기 임신기간동안 $E_2$의 농도가 상승하였다. 5. $E_2$처리 후 세포내 $Ca^{2+}$ 농도변화의 결과로, $E_2$를 처리하지 않은 난자들의 세포내 $Ca^{2+}$ 농도는 836.4$\pm$131.2 (n=10), $E_2$를 처리한 난자들은 1736.4$\pm$192.0 (n=10)로써 유의한 (P<0.05) 차이를 보였다. 이상의 결과로부터 $E_2$처리에 의한 세포내 $Ca^{2+}$ 농도 상승은 $E_2$가 $Ca^{2+}$ 통로를 자극함으로써 세포바깥의 $Ca^{2+}$이 세포안으로 이동하여 나타나는 변화로 생각된다. Steroid hormones control the expression of many cellular regulators, and a role thor estrogen in mouse oocytes has been well documented. The preovulatory $E_2$increment is generally accepted as the endocrine process regulating induction of in vivo oocyte maturation To address whether the activity of the T-type $Ca^{2+}$ channel is altered by 17 beta-estradiol ( $E_2$), we examined the actions of $E_2$on the calcium channel of mouse oocytes and early embryos. Oocrtes were collected from the oviduct of mice treated with pregnant mare's serum gonadotropin (PMSG) and human choronic gonadotropin (hCG). Whole cell voltage clamp technique and confocal microscopy were used to examine that $E_2$increase intracellular $Ca^{2+}$ concentration ([C $a^{2+}$]$_{i}$ ) via voltage dependent $Ca^{2+}$ channel (VDC) and estrogen receptor (FSR), and $E_2$concentration by the use of radioimmunoassay (RIA) were examined in mouse. The results obtained were as follows: The peak of $Ca^{2+}$ current induced by $E_2$increased 122% to 1.50$\pm$0.03 nA from 1.23$\pm$0.21 nA (n=15) in the presence of 5 mM extracellular $Ca^{2+}$ concentration ([C $a^{2+}$]$_{o}$ ). The increased $Ca^{2+}$ current was temporally associated with $Ca^{2+}$ transients. The intracellular $Ca^{2+}$ level increased 207%~30 s following the addition of 1${\mu}{\textrm}{m}$ $E_2$(relative fluorescence intensity: 836.4$\pm$131.2 for control, n=10, 1736.4$\pm$192.0 in the presence of $E_2$, n=10). $E_2$increased amplitude of $Ca^{2+}$ current and [C $a^{2+}$]$_{i}$ . $E_2$-induced $Ca^{2+}$ current and $E_2$concentration in blood were showed difference on the stage of embryo. These results suggest that $E_2$modulate $Ca^{2+}$ channel to increase $Ca^{2+}$ influx.$Ca^{2+}$ influx.

Upregulation of Glutathion S-Transferase mu 1 in Bovine Cystic Follicles

강다원,Chang-Woon Kim,한재희 사단법인 한국동물생명공학회 2010 한국동물생명공학회지 Vol.25 No.4

Follicular cystic follicles (FCFs) show delayed regression with persistent follicle growth. However, the mechanism by which follicles are persistently grown remains unclear. Glutathione S-transferases (GSTs) are drug-metabolizing and detoxification enzymes that are involved in the intracellular transport and metabolism of steroid hormones. In this study, a proteomic analysis was performed to identify whether GST expression is changed in bovine FCFs and to predict the interactions between GST and other proteins. Normal follicles and FCFs were classified based on their sizes (5 to 10 mm and ≥25 mm). In bovine follicles, GST mu 1 (GSTM1) was detected as a differentially expressed protein (DEP) and significantly up-regulated in FCFs compared to normal follicles (p<0.05). Consistent with the proteomic results, semi-quantitative PCR data and western blot analysis revealed an up-regulation of GSTM1 in FCFs. Expression levels of aromatase and dehydrogenase proteins were changed in FCFs. These results show that the up-regulation of GSTM1 that is observed in bovine FCFs is likely to be responsible for the persistent follicle growth in FCFs as the activity of aromatase and the dehydrogenases.

Synthesis and Characterization of D-π-A Copolymer with Perfluorinated TPD Acceptor for Polymer Solar Cells

강다원,엄유경,조인호,김환규 한국고분자학회 2013 한국고분자학회 학술대회 연구논문 초록집 Vol.2013 No.4

Synthesis and Characterization of D-π-A Copolymers with Perfluorinated TPD Acceptors for Polymer Solar Cells

강다원,엄유경,신원석,문상진,김환규 한국고분자학회 2014 한국고분자학회 학술대회 연구논문 초록집 Vol.2014 No.4

한우 자궁내막염에서 발현 변화를 보이는 유전자

강다원 한국동물생명공학회(구 한국동물번식학회) 2007 Reproductive & developmental biology Vol.31 No.3

본 연구는 한우의 자궁내막염에서 발현 변화를 보이는 유전자를 마이크로어레이를 이용하여 조사하였다. 정상적인 자궁내막과 자궁내막염이 있는 자궁내막을 비교한 결과, 전체 확인된 4,560개의 유전자 중 2,026개의 유전자가 자궁내막염에서 증가하였고, 2,534개의 유전자가 감소하였다. 본 연구에서는 상위 조절되는 유전자 10개씩을 정리하였다. 자궁내막염에서 filamin A, pancreatic anionic trypsinogen, Rho GDP dissociation inhibitor alpha, collagen type VI alpha 1, butyrate response factor 2, aggrecanses-2, annexin 14, aminopeptidease A, orphan transporter v7-3 및 epithelial stromal interaction 1의 발현율이 26배 이상 증가하였다. MHC class II antigen, integrin-binding sialoprotein, uterine milk protein precursor, down-regulated in colon cancer 1, glycoprotein 330, dickkopf-1, cfh protein, Ca2+-dependent secretion activator, UL16 binding protein 3 및 proenkephalin은 25.5배 이상 감소하였다. 본 연구에서 얻어진 유전자 정보는 한우의 자궁내막염 진단에 필요한 유용한 생물지표로 사용되어질 수 있을 것으로 사료된다. This study was carried out to examine gene expression altered in endometrium of Korean cattle (Hanwoo) with endometritis using microarray. In this study, 4,560 differentially expressed genes (DEGs) were identified in the endometrium of Hanwoo. Of 4,560 DEGs, 2,026 genes were up-regulated, while 2,536 genes were down-regulated in endometritis. Of them, top 10 regulated genes were listed. Filamin A, pancreatic anionic trypsinogen, Rho GDP dissociation inhibitor alpha, collagen type VI alpha 1, butyrate response factor 2, aggrecanses-2, annexin 14, aminopeptidease A, orphan transporter v7-3, and epithelial stromal interaction 1 were up-regulated, while MHC class II antigen, integrin-binding sialoprotein, uterine milk protein precursor, down-regulated in colon cancer 1, glycoprotein 330, dickkopf-1, cfh protein, Ca2+-dependent secretion activator, UL16 binding protein 3, and proenkephalin were down- regulated in the endometritis. Our results suggest that these genes could be useful biomarkers for diagnosis Hanwoo’s endometritis.

[INTERVIEW] 연주헌 UBE 융합생명과학과 교수

강다원 한국뇌과학연구원 2016 브레인 Vol.59 No.-

[뇌교육 리포트] 지구촌 미래의 열쇠, 인간의 올바른 뇌 활용에 있다!

강다원 한국뇌과학연구원 2016 브레인 Vol.59 No.-