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안개초에서 분리한 Cucumber mosaic virus의 특성
박호섭 ( Ho Seop Park ),최장경 ( Jang Kyung Choi ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2009 강원 농업생명환경연구 Vol.21 No.-
An isolate of Cucumber mosaic virus (CMV) was isolated from Gypsophila paniculata showing mosaic symptoms, and designated as Gyp-CMV. Gyp-CMV was characterized by host reaction on several indicator plants, RT-PCR analysis, restriction enzyme profile of the PCR products and amino acid sequence analysis of coat protein. Gyp-CMV differed markedly in their host reaction to Fny-CMV on Cucumis sativus and Gomphrena globosa. In the Cucumis sativus and Gomphrena globosa Fny-CMV induced typical mosaic symptoms, whereas symptoms induced by Gyp-CMV was developed symptomless infection in the plants. In experiments of dsRNA analysis, RT-PCR and RFLP profiles, properties Gyp-CMV was come within subgroup IA CMVs. Gyp-CMV was highly similar to Fny-CMV in homology of amino acid sequence of coat protein, and only one amino acid change was observed.
GM 격리포장 내 고추에서 분리한 Cucumber mosaic virus 분리주들의 외피단백질 유전자 비교
홍진성,박호섭,류기현,최장경 한국식물병리학회 2009 식물병연구 Vol.15 No.3
Twelve Cucumber mosaic virus (CMV) isolates were isolated from genetically modified (GM) and non-GM Capsicum annuum in two GM fields, Namyangju and Anseong, and their properties were investigated in this study. Coat protein (CP) gene of the CMV isolates were synthesized by RT-PCR using genus-specific primers which designed to amplify a DNA fragment of 950 bp. Purified cDNA fragments were cloned into the pGEMT easy vector for sequence determination. Nucleotide sequences (internal 657 bp) of CMV isolates were compared with Fny-CMV CP sequences and there were no significant collection site specific sequence similarities found. When predicted amino acid sequences (219 amino acids) were compared with Fny-CMV CP amino acids sequences, there were 96.8% to 97.3% similarities found from Namyangju collections and 95.9% to 96.8% similarities from Anseong collections. The phylogenetic analysis with nucleotide sequences showed definite differences in CMVs which have been isolated from the two regions.
신수진,박호섭,성유나,유창훈,황대욱,박진홍,김규표,이상수,류백렬,서동완,김송철,홍승모 대한암학회 2018 Cancer Research and Treatment Vol.50 No.4
Purpose Pancreatic cancer associated double primary tumors are rare and their clinicopathologic characteristics are not well elucidated. Materials and Methods Clinicopathologic factors of 1,352 primary pancreatic cancers with or without associated double primary tumors were evaluated. Results Of resected primary pancreatic cancers, 113 (8.4%) had associated double primary tumors, including 26 stomach, 25 colorectal, 18 lung, and 13 thyroid cancers. The median interval between the diagnoses of pancreatic cancer and associated double primary tumors was 0.5 months. Overall survival (OS) of pancreatic cancer patients with associated double primary tumors was longer than those with pancreatic cancer only (median, 23.1 months vs. 17.0 months; p=0.002). Patients whose pancreatic cancers were resected before the diagnosis of metachronous tumors had a better OS than patients whose pancreatic cancer resected after the diagnosis of metachronous tumors (48.9 months and 13.5 months, p=0.001) or those whose pancreatic cancers were resected synchronously with non-pancreas tumors (19.1 months, p=0.043). The OS of pancreatic cancer patients with stomach (33.9 months, p=0.032) and thyroid (117.8 months, p=0.049) cancers was significantly better than those with pancreas cancer only (17.0 months). Conclusion About 8% of resected pancreatic cancers had associated double primary tumors, and those from the colorectum, stomach, lung, and thyroid were common. Patients whose pancreatic cancer was resected before the diagnosis of metachronous tumors had better OS than those resected after the diagnosis of metachronous tumors or those resected synchronously.
최가영,김관중,박호섭,황은상,조재민,김현범,김대옥,박지호 한국식품과학회 2024 Food Science and Biotechnology Vol.33 No.1
Atopic dermatitis (AD) is an inflammatory skin disease showing skin barrier dysfunction, eczematous lesions, severe itching, and abnormal immune responses. The aim of this study was to determine whether an herb combination of Lithospermum erythrorhizon (LE), Houttuynia cordata (HC), and Spirodela polyrhiza (SP) has a superior anti-AD effect. Forty-two compounds were identified in LE, HC, SP, and a combined herb extract of LE, HC, and SP (LHS) using ultra-high-pressure liquid chromatography (UHPLC)-Orbitrap mass spectrometer (MS). The concentration of flavonoid glycosides including orientin (luteolin-8-C-glucoside), quercetin-3-O-rhamnoside, and luteolin-7-O-glucoside in the LHS was increased than in individual extracts. Furthermore, the treatment of LHS most effectively inhibited the increase of epidermal thickness, the number of mast cells, and the release of immunoglobulin E compared with that with each extract. These results suggest that the potential anti-AD effects of the LHS are due to the changes of bioactive compounds by the combination of herbs.
장성일,김기동,김용준,박호섭,이면우 대한인간공학회 2006 대한인간공학회 학술대회논문집 Vol.- No.-
Fun 에 관한 연구는 대부분 특정활동에 대한 동기부여를 요소로 하였고, 체계적이고 직접적인 연구는 매우 드물었다. 이번 연구에서는 Fun 을 외부적 보상이나 목적을 기대하지 않는 학습의 과정에서 새로운 패턴을 흡수할 때 두뇌가 보내는 긍정적 피드백으로 정의했다. 그리고 이를 통해 추출한 재미 요소를 가지고 AHP 를 통해 Fun 의 구조와 모델을 최종적으로 제안했다. 이번 연구로 학습의 요소를 추출했으며, AHP 를 통해 학습의 구조와 모델을 제안했다. 또한 재미 요소와 학습 요소간의 Matrix 분석을 통해 이 둘이 서로 밀접한 연관관계를 가지고 있음을 밝혔으며, 이를 기반으로 재미를 학습 요소로 표현한 재미-학습을 제안했다. 재미-학습 모델의 주요변수인 Knowledge of result(near success/near failure)을 독립변수로 최대가 되는 지점, 혹은 근처에서 재미가 최대가 됨을 증명하였다. 이러한 결과는 기존의 Usability 의 개념을 구성하는 큰 요소인 Ease of use 와 Ease to learn 이 Knowledge of results 의 Uncertainty 를 0 에 가까운 값으로 유지하려는 개념임을 고려할 때, 시사하는 바가 매우 크다고 할 수 있다.
증례보고: 이하선에 발생한 다형선종 유래 피막내 암종 1예
이승열(Seungyeol Lee),손황규(Hwangkyu Son),박호섭(Ho Sub Park),송창면(Chang Myeon Song) 대한두경부종양학회 2022 대한두경부 종양학회지 Vol.38 No.1
Among a variety of malignant types for parotid gland tumors, intracapsular carcinoma ex pleomorphic adenoma which is classified as a non-invasive tumor has been reported rarely. We report a case of a 69-years old patient, who presented with a left parotid mass that was detected 30 years ago. Fine needle aspiration biopsy result of the mass was “suggestive of pleomorphic adenoma”. Superficial partial parotidectomy was performed for the mass and the permanent pathologic finding was “intracapsular carcinoma ex pleomorphic adenoma” which was a salivary ductal carcinoma with well-preserved myoepithelial cells surrounding the malignant epithelial cell clusters. Surgical resection is the main treatment modality for the treatment of intracapsular carcinoma ex pleomorphic adenoma. Herein, we present the case with a review of literature.
신수진,박찬식,이향신,정근영,길민찬,박호섭,박영수,윤덕현,서철원,박찬정,허주령 대한병리학회 2014 Journal of Pathology and Translational Medicine Vol.48 No.3
Background: Multiple myeloma (MM) is a heterogeneous and ultimately fatal disease. Risk stratification using prognostic biomarkers is crucial to individualize treatments. We sought to investigate the role of CD99, a transmembrane protein highly expressed in many hematopoietic cells including subpopulations of normal and neoplastic plasma cells, for MM risk stratification. Methods: CD99 expression was measured in paraffin samples of bone marrow and extramedullary biopsies of 170 patients with MM. Patients were divided into those with high score (moderately andstrongly positive) and low score (negative and weakly positive), with all staining being cytoplasmic and/or membranous. Results: High anti-CD99 immunostaining was observed in 72 of 136 (52.9%) bone marrow biopsies and 24 of 87 (27.6%) extramedullary biopsies in MM. High CD99 expression of extramedullary specimens was associated with significantly longer overall survival (OS; p=.016). High CD99 expression of extramedullary specimens was also associated with better prognosis in the nonautologous stem cell transplantation group of MM patients (p=.044). In multivariate analysis, International Staging System stage was an independent prognostic factor, whereas CD99 expression was no longer statistically significant. Conclusions: Expression of CD99 in extramedullary specimens was correlated with longer OS, suggesting that CD99 may be a helpful immunohistochemical marker for risk stratification.