Successful Management of Hepatic Lipidosis Accompanied by a Feline Skin Fragility Syndrome-like Lesion in a Cat

박형진,홍은지,권효정,박성준,박주민,송근호,서경원,Park, Hyoung-Jin,Hong, Eun-Ji,Kwon, Hyo-Jung,Park, Seong-Jun,Park, Joo Min,Song, Kun-Ho,Seo, Kyoung-Won The Korean Society of Veterinary Clinics 2015 한국임상수의학회지 Vol.7 No.2

A 7-year-old spayed female Somali-cross cat was admitted with a 4-week history of anorexia, vomiting, weight loss, and lethargy. The cat was diagnosed with hepatic lipidosis. With intensive care and nutritional support via a nasogastric feeding tube for 3 weeks, the clinical signs of hepatic lipidosis were improved. However, skin lesions were found in the left and right scapular regions during the treatment that were suspected to be due to feline skin fragility syndrome (FSFS). Intensive wound healing therapy with granulated sugar, laser therapy, and a surgical flap was conducted. Skin lesions improved uneventfully without other clinical signs or recurrence of any skin lesion for a year. To our knowledge, this is the first report of a good prognosis in a hepatic disorder and concurrent FSFS. 7년령의 불임 암컷 소말리 교잡종 고양이가 4주 동안 지속된 식욕부진, 구토, 체중감소, 무기력을 주증으로 내원하였다. 환자는 지방간으로 진단 되었으며, 3주 동안 집중치료와 더불어 비위 영양관을 통해 유동식을 공급하였다. 치료기간 중, 지방간으로 인한 임상증상은 개선 되었지만 고양이 피부유약증으로 인한 것으로 의심되는 피부병변이 왼쪽과 오른쪽 어깨 부위에서 발견되었다. 과립당치료, 레이저치료 및 외과적 피판술을 통한 집중적인 상처 치유를 실시하였으며, 그 결과 환자는 다른 임상 증상이나 피부 병변의 재발 없이 성공적으로 치료되었다. 보고된 바에 따르면, 본 증례는 간질환과 고양이 피부유약증이 동반한 환자에서 좋은 예후를 보인 첫 번째 증례이다.

음이항회귀식을 이용한 도로표지의 인지반응시간 추정모형 개발

박형진,이기영,김정룡,Park, Hyung-Jin,Lee, Ki-Young,Kim, Jung-Young 대한인간공학회 2006 大韓人間工學會誌 Vol.25 No.4

The purpose of this study is to determine the economical standard of road signs by verifying the difference of driver's recognition and reaction time according to the space rate of letters on the road signs. For this reason, indoor simulations was conducted to confirm difference of recognition and reaction time on six sign-targets having different space rate. Also, a negative binomial regression model was used to find the main factors which could lower the rate of misreading. For this model, increasing of legibility of sign is not only simple enlargement of sign, but also suitable match of letters and sign. The result of this study is capable of verifying the importance of the space rate in road signs, and being utilized as a effective method to determine the standard of the road signs.

강원도 지역에 서식하는 무당개구리에서 생물학적 활성을 지닌 Bombesin 유사 Peptide에 관한 연구

박형진,이윤렬,권혁일,김일,유일재,Park, Hyoung-Jin,Lee, Yun-Lyul,Kwon, Hyeok-Yil,Kim, Yil,Yu, Il-Je 생화학분자생물학회 1992 한국생화학회지 Vol.25 No.3

본 실험에서는 강원도 지역에 집단으로 서식하고 있는 한국산 무당개구리(Bombina orientalis) 피부에서 bombesin 유사 peptide를 순수정제하여 생리활성과 amino acid 서열을 측정하고자 하였다. 무당개구리의 피부를 methanol에 담가 bombesin 유사반응물질(BLI)을 추출한 다음 alkaline alumina에 이은 Sephadex G-10 겔 크로마토그래피와 RP C18 preparative HPLC로 부분정제하였다. 부분정제시 여러 종류의 BLI가 출현하므로 본 연구에서는 전체 BLI의 65%를 점유하는 peak(BLI-K1)를 RP C18 및 SP-이온교환 HPLC를 이용하여 순수정제하였다. 피부조직 420 g에서 $890\;{\mu}g$의 BLI-K1를 순수정제하였으며, 이것은 RP C18, 겔 투과 그리고 SP-이온교환 HPLC 모두에서 합성 bombesin(BBS)과 동일한 정체시간을 나타내었다. 생리활성에서도 BLI-K1은 마취한 흰쥐에서 혈장 gastrin 농도와 혜장 단백질, 중탄산이온, amylase 및 chymotrypsin 분비량을 모두 유의하게 증가시켜 합성 BBS와 동일한 활성을 나타내었다. 또한 BLI-K1의 분자량 및 C-terminal의 11개 amino acid 서열이 합성 BBS과 동일하였기 때문에 한국산 무당개구리의 피부에도 유럽산 무당개구리의 피부에 존재하는 것과 동일한 bombesin이 존재하는 것으로 생각된다. The present study was performed to purify a BLI from the skin of frogs, B. orientalis inhabiting Kangwon-Do, Korea, and then determine its physiological activities and amino acid sequence. A substance that shows bombesin-like immunoreactivity (BLI) was partially purified from crude methanol extract of the frog skin by using a column of alkaline alumina and Sephadex G-10. BLI was further purified by using RP C18 preparative HPLC in which BLI was separated into 5 peaks. The major peak (BLI-K1) containing 65% of total BLI was subjected to purify as a single component by using sequential HPLC of SP-ion exchange and RP C18. Content of BLI in each fraction was monitored by RIA for which bombesin antiserum with a high titer and affinity to BBS was raised in a guinea pig. Eventually, $890\;{\mu}g$ of BLI-K1 was homogeneously purified from 420g of the skin of B. orientalis and it was not differentiated from synthetic BBS in RP C18, gel permeation and SP-ion exchange HPLC. In the physiological activities, BLI-K1 was identical to synthetic BBS in stimulation of gastrin release and exocrine pancreatic secretion including volume, protein, bicarbonate, amylase and chymotrypsin output in anesthetized rats. Furthermore, molecular weight and partial amino acid sequence of BLI-K1 was revealed to be identical to synthetic BBS. Therefore, it is concluded from the above results that the skin of B. orientalis contains the same BBS that has been isolated from the skin of European Bombina.

한림 21세기 계획

박형진,Park, Hyeong-Jin 한국대학교육협의회 1997 大學敎育 Vol.85 No.-

노반, 궤도분야 철도인프라 BIM 원형 라이브러리 구축

박형진,서명배,Park, Hyung-Jin,Seo, Myoung-Bae 한국전산구조공학회 2017 한국전산구조공학회논문집 Vol.30 No.5

BIM 설계에서 라이브러리의 구축 및 활용은 매우 중요하다. 기존 컨텐츠의 재활용이 가능하므로, 설계를 보다 효과적이고 효율적으로 진행할 수 있다. 건축분야와 달리 토목분야, 특히 철도시설분야에서는 라이브러리의 구축 및 표준화가 미진하다. 따라서 본 연구에서는 철도시설분야에 3차원 객체 라이브러리를 구축하고 표준화하고자 한다. 철도시설물 분류체계 및 관련도면을 수집 및 분석한다. 분석된 표준도면의 항목과 분류체계 항목을 매칭하였다. 각 항목별로 라이브러리가 필요한지 여부와 그럴 경우 어떤 소프트웨어가 적절한지를 검토하였다. 사용가능한 소프트웨어는 주로 Civil 3D와 Revit 등으로 나타났다. 향후 이를 바탕으로 철도인프라 3차원 라이브러리의 속성 항목 및 명세서를 설계하고 라이브러리를 구축할 예정이다. The introduction of BIM in domestic construction industry has become more active. Library development and standardization in civil-engineering are unexhausted. This research develop and standardize prototype library for railway infrastructure. We define target facility for library based on railway standard drawings and select BIM software according characteristic of each facility. In this research, we develop libraries composed of 199 files and 489 types for alignment, roadbed and track and make specifications as defined attribution item and description. As we consider for application to diverse use case, develop prototype library in low LoD. We expect that library can increase 3D design productivity and ensure consistency of quality.

만성 전립선염에 대한 한의학적 치료의 임상적 효과

박형진,심하나,강세영,김홍준,송봉근,심국진,Park, Hyoung-Jin,Shim, Ha-Na,Kang, Sei-Young,Kim, Hong-Joon,Song, Bong-Keun,Sim, Kuk-Jin 대한한방내과학회 2004 大韓韓方內科學會誌 Vol.25 No.4

Objectives: The prostatitis is characterized as relatively uncurable chronic recurrent illness. Because it is so difficult to treat, this study was designed to evaluate the clinical effects of herb medicine, acupuncture, and herbal acupuncture therapy on chronic prostatitis. Methods: A clinical observation was made on thirty patients with chronic prostatitis. They were treated with herb medicine, acupuncture, and herbal acupuncture therapy. Before and after treatment we measured NIH-CPSI(National Institutes of Health Chronic Prostatitis Symptom Index). Results: After treatment NIH-CPSI was decreased considerably. Conclusions: This suggests a role for oriental medical therapy in treating prostatic diseases. Further study into approach and application of these treatments for prostatitis is needed.

강원도 지역에 서식하는 무당개구리에서 생물학적 활성을 지닌 Bombesin 유사 Peptide 에 관한 연구

박형진,이윤렬,권혁일,김일,유일재,D . R . Marshak ( Hyoung Jin Park,Yun Lyul Lee,Hyeok Yil Kwon,Yil Kim,Il Je Yu,Daniel r . Marshak ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.3

The present study was performed to purify a BLI from the skin of frogs, B. orientalis inhabiting Kangwon-Do, Korea, and then determine its physiological activities and amino acid sequence. A substance that shows bombesin-like immunoreactivity (BLI) was partially purified from crude methanol extract of the frog skin by using a column of alkaline alumina and Sephadex G-10. BLI was further purified by using RP C18 preparative HPLC in which BLI was separated into 5 peaks. The major peak (BLI-K1) containing 65% of total BLI was subjected to purify as a single component by using sequential HPLC of SP-ion exchange and RP C18. Content of BLI in each fraction was monitored by RIA for which bombesin antiserum with a high titer and affinity to BBS was raised in a guinea pig. Eventually, 890 ㎍ of BLI-K1 was homogeneously purified from 420g of the skin of B. orientalis and it was not differentiated from synthetic BBS in RP C18, gel permeation and SP-ion exchange HPLC. In the physiological activities, BLI-K1 was identical to synthetic BBS in stimulation of gastrin release and exocrine pancreatic secretion including volume, protein, bicarbonate, amylase and chymotrypsin output in anesthetized rats. Furthermore, molecular weight and partial amino acid sequence of BLI-K1 was revealed to be identical to synthetic BBS. Therefore, it is concluded from the above results that the skin of B. orientalis contains the same BBS that has been isolated from the skin of European Bombina.

질병 - 닭이 주저앉는 증상에 대해서

박형진,Park, Hyeong-Jin 한국계육협회 2009 월간 닭고기 Vol.15 No.6

Prostaglandin $E_1$과 Acetazolamide가 흰쥐 전혈(全血)의 Carbonic Anhydrase 활성에 미치는 영향

박형진,조양혁,Park, Hyoung-Jin,Jo, Yang-Hyeok 대한생리학회 1980 대한생리학회지 Vol.14 No.2

This study was undertaken to investigate the influence of prostaglandin $E_1(PGE_1)$ upon the activity of carbonic anhydrase and upon the inhibitory action of acetazolamide on carbonic anhydrase. The heparinized blood was sampled by cardiac puncture from Sprague-Dawley strain rats under ether anesthesia and was hemolysed by adding distilled water 1,000 times the amount of the blood. The activity of carbonic anhydrase of 0.1 ml of the hemolysate was measured by Maren's simplified micro-method. In the first experiment, the 7 rats were used, and the activity was measured by adding 0.1 ml of various concentrations of $PGE_1$(0.5, 1.25, 2.5, 5.0, 10 and $20\;{\mu}g/ml$). In the second experiment, the 6 rats were used and the activity was measured by adding 0.1 ml of $PGE_1(5\;{\mu}g/ml)$ and 0.1 ml of acetazolamide$(6{\times}10^{-7}M/l)$ respectively or simultaneously. Obtained results were as follows: 1) The activity of carbonic anhydrase was significantly inhibited by $PGE_1$ at doses of $0.5{\sim}10\;{\mu}g/ml$, maximally at a dose of $2.5\;{\mu}g/ml$, but inhibition was no more observed at a dose of $20\;{\mu}g/ml$. 2) The activity of the acetazolamide group was significantly less than that of the control group. 3) The activity of the $PGE_1+acetazolamide$ group was significantly less than those of the $PGE_1$ group and the acetazolamide group. It is inferred from the above results that the $PGE_1$ inhibits the activity of carbonic anhydrase dose-dependently and strengthens the inhibitory effect of acetazolamide on carbonic anhydrase.

Liquid Chromatography–Solid Phase Extraction–NMR (LC–SPE–NMR) Analysis of Liquid Crystalline Mixtures

박형진,오원식,Ae-Na Park,Kyung-Rae Rho,Jong-Ho Shin,Yeongjeon Kim,Sung-Chan Jo 한국자기공명학회 2011 Journal of the Korean Magnetic Resonance Society Vol.15 No.1

We have performed Liquid Chromatography–Solid Phase Extraction–NMR (LC–SPE–NMR) analysis for liquid crystalline mixture and elucidated the structures of selected components by NMR spectra. Combining the results of one-dimensional 1H experiments as well as homonuclear and heteronuclear two-dimensional experiments, we could analyze the molecular structure of the liquid crystal singles whose structure had not been interpretable by mass spectrometry alone.