Mesangial 세포에서 고포도당에 의한 IGFs 분비와 PKC 및 산화성 스트레스와의 관련성에 관한 연구

박수현,허정선,강창원,한호재,Park, Su-hyun,Heo, Jung-sun,Kang, Chang-won,Han, Ho-jae 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.4

The proliferation of mesangial cells has been associated with the development of diabetic nephropathy. The cell proliferation has been regulated by diverse growth factors. Among them, insulin like growth factors(IGFs) are also involved in the pathogenesis of diabetic nephropathy. However, it is not yet known about the effect of high glucose on IGF-I and IGF-II secretion and the relationship between high glucose-induced secretion of IGFs and PKC or oxidative stress in the mesangial cells. Thus, we examined the mechanisms by which high glucose regulates secretion of IGFs in mesangial cells. High glucose(25 mM) increased IGF-I and IGF-II secretion. High glucose-induced increase of IGF-I and IGF-II secretion were blocked by taurine($2{\times}10^{-3}$ M), N-acetyl cystein(NAC, $10^{-5}M$), or GSH($10^{-5}M$) (antioxidants), suggesting the role of oxidative stress. High glucose-induced secretion of IGF-I and IGF-II were blocked by H-7, staurosporine, and bisindolylmaleimide I(protein kinase C inhibitors). On the other hand, high glucose also increased lipid peroxide (LPO) formation in a dose dependent manner. In addition, high glucoseinduced stimulation of LPO formation was blocked by PKC inhibitors. These results suggest that PKC is responsible for the increase of oxidative stress in the action of high glucose-induced secretion of IGF-I and IGF-II in mesangial cells. In conclusion, high glucose stimulates IGF-I and IGF-II secretion via PKCoxidative stress signal pathways in mesangial cells.

U-Home Healthcare를 위한 분산객체 미들웨어의 설계 및 구현

박수현,Park Su-Hyun 한국정보통신학회 2006 한국정보통신학회논문지 Vol.10 No.8

의료서비스 분야에서 의료와 컴퓨터 기술을 결합하여 온라인상에서 언제, 어디서나, 휴대 가능한 헬스케어(Healthcare) 장비와 유 무선 인터넷을 통해 실시간으로 환자의 생체정보 데이터를 수집하고, 이를 이용하여 진단 및 응급상황 인지가 가능한 U-헬스케어(Ubiquitous Healthcare) 시스템이 생겨났다. U-헬스케어 시스템은 기존의 의료 환경에서와는 달리, 사용자(환자, 보호자, 의료인등)들은 다양한 의료서비스를 요구하게 되고, 당연히 헬스케어 장비들은 통합된 의료서비스를 제공할 수 있도록 연동되어져야 한다. 본 논문에서 홈 네트워크(Home Network) 환경에서 사용자들의 다양한 요구를 처리하기 위해 분산 객체를 이용한 U-홈 헬스케어 미들웨어를 설계하였고, 다양한 헬스케어 장비들로부터 발생되는 생체 데이터를 통합하기 위한 데이터 변환 객체를 구현하였다. This paper proposes the U-healtcare middleware that is based on mininumCORBA. Ubiquitous healthcare system is generated by combining the technologies of computer system and medical system. This makes available that the person can receive medical treatment anywhere, anytime at on-line. The Healthcare devices are connected to network system as wire or wireless internet. So, the computer system can gather the vital information from the person at the real time and transfers the information to the server system that processes the medical information. When a medical doctor makes a diagnosis they can get more information about the patient by using the information within the server. Users would like to receive more services in the ubiquitous healthcare system than the traditional medical system. And in U-healthcare system, every healthcare devices and the users have to be connected to network system and the information from them has to be integrated. U-Home Healthcare middleware I proposed in this paper will do everything that I mentioned above.

한국십진분류법 기록관리학 분야 분류체계 개선에 관한 연구

박수현,이명규,Park, Su-Hyun,Lee, Myoung-Gyu 한국비블리아학회 2016 한국비블리아학회지 Vol.27 No.3

기록관리학이 독자적인 학문영역으로 발전해가고 있다. 하지만 KDC를 비롯한 기존의 문헌분류표에서는 분류 항목 배열이 기록관리학의 특성을 제대로 반영하지 못하여 분류항목의 전개가 불합리한 부분이 있으며, 주제영역별 세목전개의 재배치가 필요하다. 따라서 이 연구에서는 기록관리학의 학문 특성에 따라 주제영역을 기록관리 일반, 기록관리 법 정책, 기록물의 수집 선별 평가, 기록물의 조직, 기록정보서비스, 기록물 관리 및 보존, 기록관 운영, 기록관리 기관 등 8개 영역으로 설정하고, 현대 주요 문헌분류표인 KDC, DDC, NDC, UDC, LCC의 분류체계를 분석한 후, "대한민국 국가서지"의 기록관리학 분야 유별 자료현황 및 주제어 분석 결과를 반영하여 KDC 기록관리학 분야의 분류체계 수정 전개 방안을 제시하였다. 기록관리학 관련 주제영역 8개 분야의 내용은 KDC 028로 통합할 수 있도록 변경하였다. Archives and Records Management Studies is being developed its own independent domains. However, the existing library classification scheme such as the KDC don't properly reflect the characteristics of Archives and Records Management Studies. This classification scheme has the irrational part of the arrangement of the subject items and should be required to rearrange subdivision of the subject areas. In this study, According to the characteristics of Archives and Records Management Studies, It is set up 8 subject areas, Records Management (General), the law and polices of records management, the collection and appraisal of the records, the documentary organization, recording information services, preservation of the records, archives management, archives and records center, etc. After analyzing the major contemporary library classification system such as KDC, DDC, NDC, UDC, LCC, then It is suggested that improvement measures through analyzing classification status and keywords of the Archives and Records Management data contained in Korean National Bibliography. In Archives and records management studies, The contents of the eight subject areas related to the field are changed to allow integration with KDC 028.

S-100 메타데이터의 OWL기반 온톨로지 변환 구현

박수현,Park, Su-Hyun 한국정보통신학회 2011 한국정보통신학회논문지 Vol.15 No.11

해양 분야에서는 최근 e-navigation 실현을 위한 차세대 표준에 관한 연구가 진행 중이다. IHO(국제수로기구)를 중심으로 전자해도뿐만 아니라 해양의 지리 정보 및 관련 데이터의 교환, 공유, 활용할 수 있도록 S-100기반 다양한 표준에 대한 연구가 진행되고 있다. 다양한 데이터를 통합하여 안전운항을 위한 새로운 정보를 제공하기 위해서는 프로파일링을 통해 S-101, S-102, S-10x 등 다양한 표준 프로파일이 만들어지면 각 표준에서 정의하는 데이터 모델의 요소를 일관성 있고 명확하게 해석하는 것이 필요하다. 본 논문에서는 S-10x 표준의 기반이 되는 S-100 표준을 일관된 의미 해석과 처리를 할 수 있도록 S-100 표준의 메타데이터를 온톨로지로 변환하는 방안을 제시한다. In the field of maritime transport, various researches on next generation of standards are underway to realize e-navigation. While IHO leading the way, many studies on several S-100 based standards for exchange, sharing and utilizing maritime geographical information and related data are going on. IHO S-100 as a profile of ISO 19100 series of standards. Based on their application and service they provide, there can be different S-10x standards. In order to support the safe operation, various information should be integrated. When integrating a various information, it is crucial to make sure the same data model should be treated consistently and interpreted clearly across different profiles, such as S-101, S-102, etc. in this case. In this paper, I suggested a way to covert maritime metadata of S-100 to ontology so that we can perform consistent semantic analysis and processing of data in S-100 standards, which is the basis of other S-10x standards.

황연해독탕(黃連解毒湯)과 건강부자탕(乾薑附子湯)이 LPS유도에 의한 마우스 혈중 IL-6와 $TNF-{\alpha}$ 변화에 미치는 영향

박수현,권용욱,이태희,Park, Su-Hyun,Kwon, Yong-Uk,Lee, Tae-Hee 대한한의학방제학회 2007 大韓韓醫學方劑學會誌 Vol.15 No.1

Objective : This study was conducted to investigate the effects of Hwangryunhaedok-Tang and Geongangbuja-Tang on the change of interleukin-6 (IL-6) and tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) level induced by LPS I.C.V. injection in mice. Method : We devided group into 6 mice and 6 mice were assingned to each group. In the normal group only saline was administered intragastrically, and in the control group LPS was injected intracerebroventricularly 1 hr after intragastric administration of saline. In the experiment groups Hwangryunhaedok-Tang(0.5g/kg, 1.0g/kg, 3.0g/kg) was administered intragastrically to mice 1 hr prior to LPS(100mg/mouse) I.C.V. injection.. Also Geongangbuja-Tang (0.5g/kg, 1.0g/kg, 3.0g/kg) was administered intragastrically to mice 1 hr prior to LPS(100mg/mouse) I.C.V. injection. To measure the plasma IL-6 and $TNF-{\alpha}$ level of mice, their blood samples were collected from retro-orbital plexus, immediately centrifuged at $4^{\circ}C$, and plasma was removed and stored frozen at $-83^{\circ}C$ for later determination of IL-6 and $TNF-{\alpha}$. The level of IL-6 and $TNF-{\alpha}$ production was measured by enzyme-linked immunosorbent assay in the plasma. Result : Regarding IL-6 level, The 0.5g/kg and the 1g/kg groups of Geongangbuja-Tang decreased IL-6 level. Especially the 3g/kg control group decreased IL-6 level significantly than the normal group(p<0.01). Regarding $TNF-{\alpha}$ level, the 3g/kg group of Geongangbuja-Tang decreased it significantly(p<0.05). Conclusion : These data revealed that Hwangryunhaedok-Tang might not have the anti imflammatory effect and Geongangbuja-Tang(3g/kg)might have the anti imflammatory effect by reducing the plasma IL-6 and $TNF-{\alpha}$ level in mice LPS Injection.EIM (Eighteen Incompatible Medicaments) is an important component in Oriental pharmacology and is directly related to clinical prescriptions. Medical practitioners argued that the definite cause and meaning of EIM was ambiguous and therefore debated the issue of clinical application of the EIM. This study conducted an in-depth literary research on the origin, meaning and contents of EIM with the purpose to contribute in its efforts to be used clinically. Even after thousands of years have past since establishment of Oriental medicine, EIM is still tabooed and was an obstacle that hindered ideologies. Modern herbal medicine texts claim that the use of EIM can reduce treatment effects and promote poisoning and side effects. However, since long ago, there has been medical practitioners who reject this as false. Recently, poisoning caused by EIM has been claimed to be from the toxicity of the drug itself, rather than the result of interaction between the drugs, and therefore they suggest that EIM is not a forbidden domain. In addition, EIM showed a difference in number depending on the era. However, this can be understood not as a definite number, but instead as a warning to be careful during combination of drugs for use as clinical medicine. Historically, there were very few cases in which EIM was used for clinical tests and thus, the clinical value is not, while others applied EIM directly to their bodies, which showed signs for the usefulness and potential of EIM for us. A more concrete and in-depth study must be made on EIM.

방송 콘텐츠의 OSMU 활용사례 및 발전방안

박수현(Su Hyun Park),박주성(Joo Seong Park) 한국통신학회 2016 한국통신학회 학술대회논문집 Vol.2016 No.6

위성 탑재소프트웨어 검증을 위한 지상명령 생성 프로그램 설계

박수현(Su-Hyun Park) 한국항공우주학회 2017 한국항공우주학회 학술발표회 논문집 Vol.2017 No.11

외국인 전용 카지노의 관광산업적 역할과 발전방안에 대한 연구

박수현 ( Park Su-hyun ),송학준 ( Song Hakjun ) 한국호텔리조트학회(구 한국호텔리조트카지노산학학회) 2021 호텔리조트연구 Vol.20 No.1

The purpose of this study is to compare the contribution of foreigner-only casinos with other tourism industries and to present development plans for the casinos. As a result of the analysis, casinos, along with the travel agency and lodging industry, were the three largest tourism industries with the highest sales. It was found that these three industries account for about 70% of the total sales of the Korean tourism industry. Among them, casinos accounted for about 10 to 13% of the total tourism industry sales between 2012 and 2018. As of 2018, the tourism industry with the highest average number of employees was the casino with an average of 431.2 people per workplace. In addition, the casino industry showed the highest contribution to sales per capita, with an average of about 330 million won, which is three times greater than that of the lodging industry and four times that of the travel agency industry. Lastly, foreigner-only casinos were very vulnerable to international issues and incidents despite their great contribution to the national economy. In the future, it seems that tourism policies that can fully reflect these characteristics of foreigner-only casinos should be implemented.

Anti-Fibrotic Effects by Moringa Root Extract in Rat Kidney Fibroblast

Su-Hyun Park(박수현),Young-Chae Chang(장영채) 한국생명과학회 2012 생명과학회지 Vol.22 No.10

신장섬유화는 내 외부적인 요인들에 의해 발생하며, 그 요인들에 의해 염증이 생기고 지속적인 손상이 일어날 경우 신기능의 상실이 유발된다. 또한 신장섬유화는 세포 외 기질의 과다축적, TGF-β나, TNF-α, IL-1과 같은 사이토카인에 의해 발생하며, TGF-β는 신장 섬유화의 과정과 Type ? collagen과 fibronectin, PAI-1을 포함한 섬유화 관련 인자들의 발현 유도에 중요한 역할을 한다. 본 연구에서는 TGF-β를 처리한 신장섬유화 유도 모델에서 Moringa oleifera Lam 추출물에 대한 섬유화 관련 인자들의 영향을 확인하였다. 실험 결과 TGF-β로 유도된 신장 섬유화 세포에서 모링가 추출물이 fibronectin, Type ? collagen과 PAI-1의 단백질 및 mRNA 발현을 저해하였으며, 모링가 추출물 중 모링가 뿌리추출물이 가장 영향이 있는 것으로 확인 되었다. 모링가 뿌리추출물이 어떠한 기전을 통하여 섬유화 관련 인자들의 발현을 조절하는지 알아보기 위한 TGF-β로 유도된 TβR?? 및 그 하위 기전의 인산화 정도를 확인한 실험에서 모링가 뿌리추출물이 TGF-β로 유도된 TβR??과 그 하위기전의 Smad4, ERK의 인산화를 저해하였다. 그러나 TGF-β에 의해 유도된 JNK와 p38, PI3K/AKT의 인산화에는 영향이 없었다. 따라서 모링가 뿌리추출물이 TGF-β로 유도된 신장 섬유아세포에서 TβR??와 그 하위 기전인 Smad4, ERK를 통해서 Type ? collagen 과 fibronectin, PAI-1의 발현을 조절하여 섬유화를 저해 한다는 것을 예상할 수 있다. 결론적으로 모링가 뿌리추출물이 섬유화 치료 및 완화에 좋은 물질이 될 수 있을 것으로 생각된다. Fibrosis in kidney by internal and external factors causes progressive loss of renal function. Renal fibrosis is the inevitable consequence of an excessive accumulation of the extracellular matrix. TGF-β plays an important role in the process of renal fibrosis and stimulates the synthesis of profibrotic factors, including collagens, fibronectin, and plasminogen activator inhibitor (PAI-1). We examined the effect of Moringa oleifera Lam (moringa) extracts in a rat kidney fibrosis model. We found that moringa root extract suppresses protein expression/mRNA levels of Type ? collagen, fibronectin, and PAI-1 induced by TGF-β in renal fibroblasts. Moringa root extract selectively inhibited phosphorylation of TGF-β-induced TβR?? and the downstream signaling pathway (e.g., Smad4), and phospho-ERK, but not JNK, p38, or PI3K/AKT. These results suggest that moringa root extract can act against TGF-β -induced renal fibrosis in rat kidney fibroblast cells by a mechanism related to its antifibrotic activity, which regulates expression of fibronectin, Type ? collagen, and PAI-1 through TβR???Smad2/3?Smad4 and ERK. Therefore, moringa root extract is an effective substance for fibrosis therapy and provides a new therapeutic strategy for diseases associated with elevated profibrotic factor synthesis.

천공 태반에 의한 자연 자궁 파열 2예

박수현 ( Su Hyun Park ),김해숙 ( Hae Suk Kim ),김민형 ( Min Hyoung Kim ),최준식 ( June Seek Choi ),한정렬 ( Jung Yeol Han ),홍성란 ( Sung Ran Hong ),이영호 ( Young Ho Lee ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.6

Placenta percreta occurs when chorionic villi penetrate through the myometrium up to the serosa. The incidence of placenta percreta is extremely rare (about 1/140000 deliveries) but it is accompanied by life threatening complication of pregnancy due to ma