Structure of Canine Gastrin Gene

강창원,강신애,조혜련 한국유전학회 1989 Genes & Genomics Vol.11 No.4

The full length canine (dog) gastrin gene was cloned from a genomic library by in situ plaque hybridization and mapped with several restriction endonucleases. Two clones containing 12.5 kb and 14.6 kb genomic DNA fragments were isolated among over 500,000 recombinant phages by using the canine gastrin cDNA as a hybridization probe. Restriction mapping of the clones reveals that they share the identical 11.0 kb fragment containing gastrin gene of 2.5 kb. Comparison of partial genomic sequence with cDNA sequence reveals three exons and two introns like human gastrin gene. Splicing junctions are identical to the human and follow the GU/AG consensus sequence rule of mRNA processing. The canine intron I, however, is only 1.7 kb long unlike the human 3.5 kb intron I. The 5’ flanking region of the gene contains the conserved sequence T-A-T-A-A, which is present at 26 bp upstream from the putative cap site. The sequence G-A-C-T-C-A-T-A-T, found at 86 bp upstream in the human and other eukaryotic genes, is not detected. The sequence T_5-A₂-T-A-T₄-A-C-T₂-A-T₃-A-T₃-A-T₃, similar to a human RNA polymerase II terminator sequence T_9-A₂-T_5-A-T₄-A-T₄-A-T₄, appears to be the canine terminator. However, it has a inverted repeat of 6.5 bp sequence instead of the human 10.5 bp repeat unit.

이전지역 경제 활성화 및 동반성장 방안

강창원 한국지방정부학회 2014 한국지방정부학회 학술대회자료집 Vol.2014 No.8

백서 간 관류모델에서 forskolin이 Insulin like growth factor-I의 분비에 미치는 효과

강창원,이대열,이호일,Kang, Chang-won,Lee, Dae-yeol,Lee, Ho-il 대한수의학회 1999 대한수의학회지 Vol.39 No.5

The insulin-like growth factor-I(IGF-I) is an important metabolic factor involved in cell growth and metabolism. Although secretion of IGF-I in rat liver is regulated by growth hormone, the effects of forskolin, adenylate cyclase activator, on secretion of IGF-I have not been reported. Therefore, a modified perfused rat liver model was used to investigate the regulatory effects of forskolin on IGF-I secretion in this experiment. The results were summerized as follows : 1. Modified perfused rat liver model was not changed to aspartate aminotransferase(AST), alanine aminotransferase(ALT) and lactic dehydrogenase(LDH) secretion in time. 2. The IGF-I secretion in hepatic cell was increased by forskolin($10^{-5}$, $10^{-6}$ and $10^{-7}M$) in a dose-dependent manner as compared with those of the controls, and significantly increased by $10^{-5}$ and $10^{-6}$ forskolin(p < 0.05). 3. Secretion of glucose in hepatic cell significantly was decreased by $10^{-5}$ forskolin as compared with those of controls(p < 0.05). These results suggest that forskolin may be involved in the regulation of IGF-I secretion in the perfused rat liver.

개 gastrin 유전자의 클로닝과 exon - intron 의 제도

강창원,유욱준,조혜련,강신애 생화학분자생물학회 1993 BMB Reports Vol.17 No.3

The canine gastin gene was isolated from a genomic library. Screening of about 500,000 recombinant phages was carried out by in situ plaque hybridization employing a canine gastrin cDNA clone as a probe. As a result two different genomic clones were obtained. These contain 13-kb and 15-kb genomic DNA fragments that share at least 11-kb DNA. Comparison of the determined genomic sequences with the cDNA sequence revealed the structure of three exons and two introns, which comprise 2.3 kb in total. It resembles the human gastrin gene structure. However, the canine intron I is only 1.7 kb long, about half the size of the human intron I.

심방 이뇨호르몬의 분비조절에서 Ca²⁺이 protein kinase C 활성화에 미치는 영향

강창원,김진상,이호일,Kang, Chang-won,Kim, Jin-shang,Lee, Ho-il 대한수의학회 1999 대한수의학회지 Vol.39 No.5

Atrial natriuretic peptide(ANP) is a hormone with potent natriuretic, diuretic and relaxing properties on vascular smooth muscle. Specific chemical modulator in response for the ANP secretion has not been found yet. Therefore, we have investigated the role of $Ca^{2+}$ responsible for the regulation of ANP induced by protein kinase C(PKC) on mechanically stretch-induced ANP secretion in the rat atria. The results obtained were as follows ; 1. ANP secretion and ANP concentration were increased to more in $Ca^{2+}$-free buffer than in the Kreb-Henseleit buffer on mechanically stretch-induced ANP secretion(p < 0.05), but extracellular fluid translocation(ECF) was not significant. Phorbol 12-myristate 13-acetate(PMA, $10^{-7}M$) induced ANP secretion and ANP concentration in $Ca^{2+}$-free buffer shown to more accentuate on mechanically stretch-induced ANP secretion than in the $Ca^{2+}$-free buffer(p < 0.05), but ECF translocation was not significant. 2. In the presence of ryanodine($3{\times}10^{-6}M$), PMA($10^{-7}M$) induced ANP secretion and ANP concentration in the Kreb-Henseleit buffer were shown to more increase on mechanically stretch-induced ANP secretion than in the ryanodine($3{\times}10^{-6}M$) with the Kreb-Henseleit buffer(p < 0.05), but ECF translocation was not significant. 3. In the presence of ryanodine($3{\times}10^{-6}M$), PMA($10^{-7}M$) induced ANP secretion and ANP concentration in the $Ca^{2+}$-free buffer was shown to more increase on mechanically stretch-induced ANP secretion than in the ryanodine($3{\times}10^{-6}M$) with the $Ca^{2+}$-free buffer on mechanically induced ANP secretion(p < 0.05), but ECF translocation was not significant. The results suggest that PKC-induced ANP secretion may not be related to the change of $Ca^{2+}$ on mechanically induced ANP secretion in the rat atria.

R&D위원 칼럼 - 어려울수록 축산.사료 교육 투자를 높이자

강창원,Gang, Chang-Won 한국사료협회 2012 飼料 Vol.56 No.-

R&D위원 칼럼: 우리 축산의 쓰나미-그 후의 사료업계

강창원,Gang, Chang-Won 한국사료협회 2011 飼料 Vol.50 No.-

Accelerated life test plans with optimum censoring time

강창원,강창욱 대한산업공학회 1996 대한산업공학회 추계학술대회논문집 Vol.1996 No.10

Overexpressed Chloramphenicol Acetyltransferases Are Soluble but Mostly Inactive

강창원,김한복 한국유전학회 1989 Genes & Genomics Vol.11 No.4

We constructed an E. coli strain that produces chloramphenicol acetyltransferase (CAT) in high yield by utilizing the phage T7 RNA polymerase's stringent recognition and active transcription of T7 promoter. The T7 expression system was constructed such that the T7 RNA polymerase gene is under the control of lacUV5 promoter in one plasmid, and that the target gene, a promoterless CAT gene along with E. coli ribosome binding site is under the control of a T7 promoter in the other plasmid. Without induction the T7 expression system is repressed by lacI repressor. The T7 RNA polymerase produced by IPTG induction is capable of specific and efficient transcription of the CAT gene. Upon IPTG induction CAT is produced up to 36% of the total protein content. The overproduced CAT proteins are soluble but mostly inactive, as assayed for specific activity of the protein.

Effects of activation of protein kinase C on the regulation of atrial natriuretic peptide(ANP) by isolated perfused left atria

강창원,강형섭,이호일,Kang, Chang-won,Kang, Hyung-sub,Lee, Ho-il The Korean Society of Veterinary Science 1997 대한수의학회지 Vol.37 No.4

심방근 세포는 심방이뇨호르몬을 합성, 저장 그리고 분비하며, 세포내외 이온의 농도, 수분균형 및 혈압 등을 조절하는 것으로 알려져 있다. 또한 심방근의 인장자극에는 Atrial Natriuretic Peptide(ANP)를 2단계(분비, 유리)의 과정으로 이루어져 있으며, 이에 따른 심방이뇨호르몬의 분비 조절기전에 대하여서는 명확히 알려져 있지 않다. 따라서 본 연구는 백서의 심방근 적출관류 모델을 이용하여 protein kinase C와 ANP 조절의 상관관계를 밝히고 분비와 유리의 과정중 어떠한 과정을 이용하여 분비자극에 영향을 주는지를 관찰하기 위하여 본 실험을 실시하였다. PKC 활성제인 PMA(phorbol 12-mystrate 13-acetate)는 ANP의 유리를 현저하게 증가시켰으며, PKC 억제제인 H-7(1-(5-isoquinoline sulfonyl)-2-methyl piperazine dihydrochlo-ride)에 의해 유리를 억제시켰다. PMA와 H-7을 동시에 처리한 경우 PMA에 의하여 증가된 ANP의 유리가 H-7에 의하여 차단됨을 관찰할 수 있었다. 따라서 백서의 관류 심방에서의 ANP 분비 증가는 PKC 활성화에 의하여 이루어지며, ANP분비의 2단계중 ANP 유리에 영향을 줌을 알 수 있었다.