A539 및 NCI-H460 인체 폐암세포의 증식 및 apoptosis 유도에 미치는 가미삼기보폐탕의 영향

김진영,김현중,정광식,박철,최영현,감철우,박동일,Kim, Jin-Young,Kim, Hyun-Joong,Jung, Kwang-Sik,Park, Cheol,Choi, Yung-Hyun,Kam, Cheol-Woo,Park, Dong-Il 대한한방내과학회 2008 大韓韓方內科學會誌 Vol.29 No.1

Objective : This study was designed to investigate the effect of the water extract of Gamisamgibopae-tang(GMSGBPT), an oriental herbal formulation, on the growth of NCI-H460 and A549 human non-small-cell lung cancer cell lines. Methods : Cytotoxicity and cell morphology were evaluated by MTT assay and inverted microscope, respectively. Apoptosis was detected using agarose gel electrophoresis and flow cytometer. The expression levels of mRNAs and proteins of target genes were determined by RT-PCR and western blot analyses, respectively Result and Conclusion : We found that exposure of A549 cells to GMSGBPT resulted in the growth inhibition in a dose-dependent manner as measured by MTT assay, but GMSGBPTdid not affect the growth of NCI-H460 cells. The anti-proliferative effect of GMSGBPT treatment in A549 cells was associated with morphological changes, formation of apoptotic bodies and DNA fragmentation, and flow cytometry analysis confirmed that GMSGBPT treatment increased the populations of apoptotic-sub G1 phase. Growth inhibition and apoptotic cell death by GMSGBPT were connected with a up-regulation of cyclin-dependent kinase inhibitor p21 (WAF1/CIP1) mRNA and protein in a tumor suppressor p53-independent fashion. However GMSGBPT treatment did not affect other growth regulation-related genes such as early growth response-1 (Egr-1), nonsteroidal anti-inflammatory drug (NSAID)-activated gene-1 (NAG-1), inducible nitric oxide synthase (iNOS), cyclooxygenases (COXs), telomere-regulatory factors in A549 orNCI-H460 cells. Taken together, these findings partially provide novel insights into the possible molecular mechanism of the anti-cancer activity of GMSGBPT.

Effect of 3D print orientation and layer thickness on the accuracy of printed models by DLP and SLA printers

Yung-Soo Kim(김영수),Ji-Chang Song(송지창),Seung-Ho Baek(백승호),Ryan Jin Young Kim(김진영) 대한치과재료학회 2022 대한치과재료학회지 Vol.49 No.1

The aim of this study was to evaluate the influence of print layer thickness and orientation of two 3D printers on the trueness of printed models. Two different models (cube and tooth form models with class II cavity) were printed with a DLP printer (IMC, Carima) and an SLA printer (Form 3, Formlabs) with different layer thickness and printing orientation (n=12). The printed models were scanned to obtain STL datasets. Trueness of the printed models relative to the reference dataset was obtained using the superimposition technique. The surface of the 3D printed models was evaluated by SEM. One-way ANOVA and Tukey post hoc were performed. For the cube model, the overall mean trueness values were lowest by the DLP printer at 0-degree orientation in 50 μm layer thickness (20.49 μm), while the highest deviation was observed with the SLA printer at 45-degree orientation in 100 μm layer thickness (61.03 μm) (p<0.001). For the tooth-shaped model, the lowest deviation was observed with the SLA printer at 0-degree orientation in 50 μm layer thickness (25.63 μm), while the highest deviation was found with the DLP printer at 45-degree in 100 μm layer thickness (47.56 μm) (p<0.001). In SEM image, the SLA printer exhibited a relatively smoother surface compared to the DLP printer. In conclusion, the trueness was affected by the type of 3D printer, the geometry of the printing model, the thickness of the printing layer, and printing orientation for each printer.

수부 인지 신전건의 중수지 관절부에서의 외상성 탈구

성진형(Jin-Hyung Sung),김진영(Jin-Yung Kim),김원유(Won-Yoo Kim),박정수(Jeong-Soo Park) 대한정형외과학회 1995 대한정형외과학회지 Vol.30 No.4

질량스펙트럼의 펩타이드 분자량 오차범위 재해석에 의한 단백질 동정의 성능 향상

권경훈,김진영,박건욱,이정화,백융기,유종신,Gwon, Gyeong-Hun,Kim, Jin-Yeong,Park, Geon-Uk,Lee, Jeong-Hwa,Baek, Yung-Gi,Yu, Jong-Sin 한국생물정보시스템생물학회 2006 Bioinformatics and Biosystems Vol.1 No.2

In proteomics research, proteins are digested into peptides by an enzyme and in mass spectrometer, these peptides break into fragment ions to generate tandem mass spectra. The tandem mass spectral data obtained from the mass spectrometer consists of the molecular weights of the precursor ion and fragment ions. The precursor ion mass of tandem mass spectrum is the first value that is fetched to sort the candidate peptides in the database search. We look far the peptide sequences whose molecular weight matches with precursor ion mass of the mass spectrum. Then, we choose one peptide sequence that shows the best match with fragment ions information. The precursor ion mass of the tandem mass spectrum is compared with that of the digested peptides of protein database within the mass tolerance that is assigned by users according to the mass spectrometer accuracy. In this study, we used reversed sequence database method to analyze the molecular weight distribution of precursor ions of the tandem mass spectra obtained by the FT LTQ mass spectrometer for human plasma sample. By reinterpreting the precursor ion mass distribution, we could compute the experimental accuracy and we suggested a method to improve the protein identification performance. 프로테오믹스에서 얻는 탄뎀 질량 스펙트럼은 효소로 가수분해된 펩타이드의 전구이온(precursor ion) 분자량과 펩타이드에 에너지를 가하여 생성된 이온조각(fragment ion)들의 분자량값들로 구성된다. 탄뎀 질량스펙트럼의 전구이온 분자량은 단백질 서열 데이터베이스에서의 검객 과정에서 가장 먼저 고려하는 값이다. 단백질 검색 프로그램은 단백질 서열 중에 스펙트럼의 전구이온으로부터 계산된 분자량과 일치하는 펩타이드 서열들을 찾아내고, 이들 중의 하나를 이온조각들의 분자량 정보를 이용해서 선택한다. 이 때에 전구이온의 분자량은 사용자가 지정한 오차범위 내에서 일치하는 감을 검색하는데, 이때의 오차범위는 질량분석기의 정확도에 따라 결정된다. 본 논문에서는 인간 혈액의 혈장시료로부터 FT LTQ 질량분석기를 통해 얻어진 탄뎀 질량 스펙트럼에서 전구이온 분자량의 분포를 역순서열을 이용하여 분석하였다. 전구이온 분자량의 분포를 재해석하여 실험값의 정확도를 보정하고 단백질 동정의 성능을 향상시키는 방법을 모색하였다.

시계열 분석 기법을 이용한 태양광 발전량 변동성 예측

이영섭(Yung-Seop Lee),이동현(Donghyun Lee),정아현(Ahyun Jung),김진영(Jin-Young Kim),김창기(Chang Ki Kim),김현구(Hyun-Goo Kim) 한국태양에너지학회 2019 한국태양에너지학회 학술대회논문집 Vol.2019 No.4

α+β 타이타늄 합금의 미세조직 제어에 따른 기계적 특성

황유진 ( Yu-jin Hwang ),박양균 ( Yang-kyun Park ),김창림 ( Chang-lim Kim ),김진영 ( Jin-yung Kim ),이동근 ( Dong-geun Lee ) 한국열처리공학회 2016 熱處理工學會誌 Vol.29 No.5

The mechanical properties of Ti-6Al-4V can be improved by microstructural control through the heat treatment in α+β region. The heat treatment was carried out with a variety of heat treatment temperatures and holding times to find the optimized heat treatment conditions and it was analyzed by linking the microstructural characteristics and mechanical properties. The part of β phase with 10 ± 2 wt% vanadium was transformed into α```` martensite phase after quenched, so the hardness and tensile properties were decreased below 900℃. The higher the heat treatment temperature is, the smaller is the vanadium-rich region, which leads to transformation into hcp α`` martensite above 900℃. The hardness and tensile properties were improved due to the hard α`` martensite. As the holding times were longer, the hardness and tensile properties decreased below 900℃ because of the softening effect by the grain growth. When varying the holding times above 900℃, the change of mechanical properties was slight because the softening effect of grain growth and the strengthening effect of α`` phase were counteractive. Therefore, the best conditions of heat treatment, which is in the range of 920~960oC, 40 min, WQ, can effectively improve the mechanical properties of Ti-6Al-4V. (Received August 5, 2016; Revised August 25, 2016; Accepted September 5, 2016)

급성 혈슬관절증에 대한 국소 마취하의 관절경 검사

성진형,김영균,김진영,이성근 ( Jin Hyung Sung,Yung Kyun Kim,Jin Yung Kim,Seong Keun Lee ) 대한슬관절학회 1994 대한슬관절학회지 Vol.6 No.2

The degree of severity of an injury of the knee joint is estimated on the basis of the history given by the patient, stability testing and hemarthrosis, but without using MRI and CT scan,the accurate diagnosis is difficult by plain radiograph only.The early diagnosis and appropriate treatment of the injuries are very important in order to prevent disability. The objective of this study was to define the mle of early diagnostic and therapeutic knee arthroscopy under local anesthesia for patients with an acute hemarthrosis without instability, Arthmscopy under local anesthesia was performed in twenty-three patients with an acute hemarthrosis from April 1993 to September l994. All patients were males and their q@an age was 22.6 years (between 21 and 29 years of age). Main cause of injuries was sports. Mean d 'on betwaan bautna and operation was 5.9 days(range fmm I to 14 days) and mean doration between admission and o 'on was 3 daygrange from adm. Day to 8 days). 30ml of 0,25/o bupivacaine mixed with epinephrine(1:200,000) was injected into the knee joint as a local aaesdetics at tweaty minutes before opereion, The portals of entry were infiltrated witb 10 15ml of the mixture of 1% lidocaine and epinephrine (1:200,000). Injury of the anterior cruciate ligament was found in 10 cases(43,5%) and meniscal tears were noted in 15 patients(65.2%), 3 patients had an osteochondral iracture and 4 patients had a capsular tear. Combined injuries were found in 9 patients(39,1%). Partial or subtotal meniscectomics were performed in 12 cases of the 15 meniscal injuries and multiple drillings were performed in 3 cases of osteochondral fractme, In the 6 cases of injury of anterior cruciate ligament that require surgical treatment, primary repairs under general anesthesia were performed in 3 cases and secondary reconstructive operations were recommanded in the remained 3 cases, Mean time of operation was 48 minutes. No side effect of local aoesthesia was found during intra or postoperative period. W'e conclude that a traumatic hemarthrosis suggests a significant knee injury and arthmseopy uoder local anesthesia is a simple, safe and economie diagnostic and therapeutic method,

아동의 주의력 향상을 위한 홍삼 기능성 제품의 효과

김승경(Seung-Kyoung Kim),이지선(Ji-Sun Lee),김진영(Jin-yung Kim),강문희(Moon-Hee Kang),장매희(Mae-Hee Chiang) 한국원예학회 2007 한국원예학회 학술발표요지 Vol.2007 No.6

불필요한 식을 찾는 2단계 언어의 간섭분석

조성근(Sungkeun Cho),김진영(Jin Yung Kim),이광근(Kwangkeun Yi) 한국정보과학회 2011 한국정보과학회 학술발표논문집 Vol.38 No.2A

이 논문에서는 정적으로 2단계 언어의 불필요한 식을 안전하게 찾는 간섭분석 방법을 제안한다. 불필요한 식이란 식의 실행이 전체 프로그램의 실행 결과에 영향을 미치지 않는 식을 말하고 간섭분석이란 프로그램의 최종 결과에 영향을 미치는 식들을 찾아내는 분석을 말한다. 정적으로 불필요한 식을 찾으면 전체 프로그램 실행에 드는 비용을 줄일 수 있다. 이 분석은 타입 시스템에 올라탄 분석에 기반한다. 타입 규칙을 디자인하고 안전성을 증명하였으며 이를 위한 알고리즘을 디자인하고 올바름을 증명하였다. 또한 다형 타입을 지원하도록 타입 규칙을 확장하였으며 그 안전성도 증명하였다.

금불초 추출물의 항산화 효과 및 산화 스트레스에 대한 신경세포 보호작용

이나현(Na Hyun Lee),홍정일(Jungil Hong),김진영(Jin Yung Kim),장매희(Mae-Hee Chiang) 한국식품과학회 2009 한국식품과학회지 Vol.41 No.1

본 연구에서는 금불초(Inula britannica) 추출물의 항산화 효과와 H₂O₂로부터 유도된 SH-SY5Y 신경모세포종의 세포독성에 대한 보호능을 측정하였다. 금불초 지상부위의 70% 메탄올 추출물에 대하여 용매별로 분획을 실시하였고 핵산(Fr.H), 에틸아세테이트(Fr.EA) 및 물(Fr.W) 분획에 대하여 활성을 조사하였다. 분획중 Fr.W의 폴리페놀/플라보노이드 함량이 가장 높았으며 Fr.W의 총 폴리페놀 함량은 318.1±20.6 ㎍/㎎ solid로, Fr.EA 및 Fr.H와 비교하여 각각 약 2.5배, 23.1배 수준이었다. DPPH radical, ABTS radical 및 nitric oxide 소거능 등의 항산화 활성에서도 Fr.W가 가장 높은 활성을 나타내었고 Fr.H는 거의 활성을 나타내지 않았다. Fr.W는 H₂O₂에 의해 유도된 세포사멸에 대하여 62.5 ㎍/mL 농도에서 현저하게 세포독성을 감소시켰으며 250 ㎍/mL에서는 77.0%의 세포사멸 억제능을 보였다. Fr.EA는 보호 효과를 나타내지 않았으며 Fr.H는 오히려 H2O2로 인한 세포 독성을 증가시키는 것으로 나타났다. 세포 내 ROS에 대한 영향으로 Fr.W 250 ㎍/mL 처리시 39.2% 세포내 ROS를 감소시켰으며 Fr.EA는 25 ㎍/mL에서 26.8%의 세포내 ROS를 소거하였다. 이러한 금불초 Fr.W의 항산화 활성은 ROS에 의해 야기되는 뇌세포 독성에 대한 보호 작용에 공헌할 수 있을 것으로 예상된다. The antioxidant properties and protective effects of Inula britannica on H₂O₂-induced SH-SY5Y neuroblastoma cell damage were investigated. A series of solvent fractions, including hexane(Fr.H), petroleum ether, chloroform, ethyl acetate(Fr.EA), and water fraction(Fr.W), were prepared from the 70% methanol extracts of Inula britannica. Fr.W had the highest total contents of phenolics and flavonoids, followed by Fr.EA. The antioxidant properties of the fractions were also evaluated by analyzing their scavenging activities on 1,1-diphenyl-2-picrylhydrazyl(DPPH) radicals, 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals, and nitric oxide. Fr.W showed the strongest activities in all assays. The concentrations of Fr.W that resulted in 50% reductions of the DPPH and ABTS radicals were 20.7 ㎍/mL and 39.4 ㎍/mL, respectively. Fr.W showed the weakest cytotoxic activities on the SH-SY5Y cells, whereas it effectively protected H₂O₂-induced cell death, increasing cell survival by 35.0-77.0% at a concentration range of 62.5-250 ㎍/mL. In this range, Fr.W also significantly decreased intracellular ROS levels by 34-39%. Overall, the antioxidant properties of Inula britannica can contribute to rescuring neuronal cells from oxidative stress-induced cell injury.