역기하렌즈 착용 전 요인들이 착용 후 시력에 미치는 영향

김성훈,이정훈,김춘식,Sung Hoon Kim,Jung Hoon Lee,Chun Sik Kim 대한안과학회 2010 대한안과학회지 Vol.51 No.10

Purpose: To determine whether patient factors (pre-fitting patient’s data) before reverse-geometry lens (RGL) use could be predicting factors for the increase in visual acuity after RGL use. Methods: The authors reviewed out patient records of 805 eyes of 423 patients wearing RGLs from March 2003 to May 2009. The relationship between patient factors on UVA before wearing a RGL examined at follow-ups and the IVA after wearing a RGL were analyzed. Results: The spherical refractive errors and the cylindrical refractive errors showed statistically significant results (p < 0.05). The lower was the refractive error, the greater were the UVA and IVA results. Correlations existed between the corneal astigmatism and UVA or IVA at three months (p < 0.05). The lower was the corneal astigmatism, the greater were the UVA and IVA results. Correlations were observed between the initial visual acuity and IVA, although the initial visual acuity cannot be a predictor. The other factors evaluated did not show statistically significant results (p > 0.05). Conclusions: The refractive error and the corneal astigmatism may be strong predictors of UVA and IVA after RGL use. J Korean Ophthalmol Soc 2010;51(10):1305-1311

$^{13}C$ Nuclear Magnetic Resonance Study of Graphite Intercalated Superconductor $CaC_6$ Crystals in the Normal State

김성훈,강기혁,민병진,이무희,김준성,Kim, Sung-Hoon,Kang, Ki-Hyeok,Mean, B.J.,Ndiaye, B.,Lee, Moo-Hee,Kim, Jun-Sung The Korean Superconductivity Society 2010 Progress in superconductivity Vol.12 No.1

$^{13}C$ NMR (nuclear magnetic resonance) measurements have been performed to investigate the local electronic structure of a superconducting graphite intercalation compound $CaC_6$ ($T_c$ = 11.4 K). A large number of single crystals were stacked and sealed in a quartz tube for naturally abundant $^{13}C$ NMR. The spectrum, Knight shift, linewidth, and spin-lattice relaxation time $T_1$ were measured in the normal state as a function of temperature down to 80 K at 8.0 T perpendicular to the c-axis. The $^{13}C$ NMR spectrum shows a single narrow peak with a very small Knight shift. The Knight shift and the linewidth of the $^{13}C$ NMR are temperature-independent around, respectively, +0.012% and 1.2 kHz. The spin-lattice relaxation rate, $1/T_1$, is proportional to temperature confirming a Korringa behavior as for non-magnetic metals. The Korringa product is measured to be $T_1T\;=\;210\;s{\cdot}K$. From this value, the Korringa ratio is deduced to be $\xi$ = 0.73, close to unity, which suggests that the independent-electron description works well for $CaC_6$, without complications arising from correlation and many-body effects.

홍바리, Epinephelus fasciatus 정자의 미세구조

김성훈,이치훈,송영보,주해성,김형배,이영돈,Kim, Seong-Hoon,Lee, Chi-Hoon,Song, Young-Bo,Ju, Hea-Sung,Kim, Hyung-Bae,Lee, Young-Don 한국현미경학회 2012 Applied microscopy Vol.42 No.2

Ultrastructural characteristics of blacktip grouper, Epinephelus fasciatus spermatozoa were investigated using transmission and scanning electron microscopy. The spermatozoa of E. fasciatus consisted of a spherical head part, a midpiece with cytoplasmic canal entrance and a flagellum with lateral fins. Internal ultrastructurally, the nucleus contains high electron dense chromatin having granular particles and has no acrosome. The centriolar complex lies outside of the nuclear fossa and it is connected by the osmophilic filaments. Also the osmophilic filaments connect between the centriolar complex and the nuclear membrane. The midpiece contains eight to nine spherical mitochondria, cytoplasmic canal and necklaces. The flagellum has a typical 9+2 axonemal structure. The lateral fins contain vesicles and a typical 9+2 axonemal structure. Consequently this study contributes to comparative grouper spermatology and provide useful systematic taxonomic characters. 홍바리, Epinephelus fasciatus 정자의 미세구조적 특징을 주사형 및 투과형 전자현미경으로 관찰하였다. 홍바리 정자는 구형의 두부와 cytoplasmic sleeve와 고리형 cytoplasmic canal 입구 등으로 구성된 중편부 그리고 lateral fin을 갖는 1개의 편모로 구성된 미부로 구별된다. 정자의 핵은 구형이며, 첨체 구조물은 존재하지 않으나 이질 염색질이 과립상 덩어리 형태로 조밀하게 분포하였다. Proximal centriole과 distal centriole은 각각 수레바퀴형의 "9+0"의 축사구조를 보이며 서로 수직으로 osmophilic filaments 연결되어 있고, 핵막과도 연결되어 있었다. 중편부는 구형의 8~9개의 미토콘드리아를 포함하며, 빈공간인 cytoplasmic canal과 편모 원형질막에는 7~8개의 구형 입자를 갖는 necklace가 존재하였다. 미부는 1개의 편모를 갖으며 전형적인 "9+2"의 축사구조를 보였고, 편모에는 다양한 형태의 lateral fins을 관찰할 수 있었다. 홍바리 정자의 미세구조적 특징은 바리과 어류의 정자와 상이하여 계통분류학적 동정의 형질이 될 수 있을 뿐만아니라 정자 세포소기관의 기능적 및 생리학적 연구에 이용될 수 있다고 판단된다.

홍바리, Epinephelus fasciatus의 인공수정 전.후 난문의 미세구조적 변화

김성훈,이치훈,주해성,김형배,이영돈,Kim, Seong-Hoon,Lee, Chi-Hoon,Ju, Hea-Sung,Kim, Hyung-Bae,Lee, Young-Don 한국현미경학회 2011 Applied microscopy Vol.41 No.2

홍바리, Epinephelus fasciatus 난의 인공수정 전 후 난문의 미세구조적 특징을 주사형 전자현미경으로 관찰하였다. 홍바리 난문($6.6{\pm}0.41\;{\mu}m$)은 원통형의 분화구형으로 동물극에 1개가 존재하였다. 난문의 vestibular ridge는 시계방향성을 갖는 6~7개의 나선형의 내벽구조로 구성되었다. 난문의 내벽구조물인 나선형의 vestibular ridge는 인공수정 후 부풀어 올라와 돔(dome)형의 수정구로 분화 형성되었다. 홍바리 난은 부상란으로 난막표면에는 pore 가장자리에 고리형의 nodule과 pore 사이에 돌기형의 nodule이 관찰되었으나, 부착사와 유사한 보조 구조물은 관찰할 수 없었다. 난문 주변부에는 동심원상으로 크기가 다른 pore (0.15~0.55 ${\mu}m$)들이 개구된 형태로 혼재하였고, 수정 후 돔(dome)형의 수정구가 분화 형성되면서 pore들이 닫히는 현상을 보였다. 홍바리 난문의 미세구조적 특징은 바리과 어종간에 형태학적으로 상이하여, 바리과 어류의 계통분류학의 형질로 이용될 수 있다고 판단된다. The micropyles on the prefertilized and artificial fertilized eggs of Epinephelus fasciatus were investigated using scanning electron microscope (SEM). The micropyles ($6.6{\pm}0.41\;{\mu}m$) of E. fasciatus eggs were found in the animal pole and theirs shape were observed as a flat crateriform of cylindrical shape. The micropylar vestibule arranged by 6~7 thickened spiral annuli on the ridge and contributed to differentiate and form fertilization cone for blocking to polyspermy by presenting swollen vestibule structure. As E. fasciatus eggs was pelagic, so chorionic surface was an uneven structures such as circular and fillar form nodules. Especially, various pores (0.15~0.55 ${\mu}m$, 230~270 pores) distributed at the only around micropyles, those pores radiately exhibited regular projection structures showing gill filament-shape. These ultrastructural characters of E. fasciatus eggs can be utilized in a taxonomical cue of grouper species.

Q-행렬에 근거하는 치유교습 순서

김성훈(Sung Hoon Kim) 한국교육평가학회 2020 교육평가연구 Vol.33 No.2

이 연구는 인지진단 결과로 주어지는 미성취 인지요소의 치유교습 순서에 Q-행렬이 어느 정도로 어떻게 결정하는지 탐색하였다. Tatsuoka(2009)가 소개한 인접행렬, 도달행렬, 그리고 영향도표를 이용하여 Q-행렬에 내재하는 인지요소 간의 인과관계를 치유교습의 순서로 개념화하였다. 이 연구 결과로 제안한 Q-행렬의 치유교습 순서 기여도는 그 행렬에 포함된 K개의 인지요소 간 있을 수 있는 모든 인과관계의 수에 대한 실제 Q-행렬에 내재하는 인과관계 수의 비율로 정의하였다. 분석 대상 Q-행렬은 이 이론적 배경에 사용된 예시적 Q-행렬과 실제 Q-행렬 2개다. 후자는 성태제(2018) 연구와 박세진·이현숙(2018) 연구에서 인용하였다. 연구 결론으로서 Q-행렬은 치유교습 순서 결정을 위한 보편적 출발점이 되며; 지식의 위계가 분명한 검사일수록 Q-행렬의 치유교습 순서 기여도는 높을 것이며; 인지진단 결과로 주어지는 인지상태는 Q-행렬의 가정과 충돌할 수 있음 등이 논의되었다. This study was to investigate how and how much does a Q-matrix contribute in deciding the sequence of remedial instruction of the unattained attributes being identified from cognitive diagnostic assessment. Applying the adjacency matrix, reachability matrix, and influence diagram introduced in cognitive assessment context by Tatsuoka (2009), the sequence was defined as the causal relationship among attributes forming a Q-matrix. Three Q-matrices were gone under analysis. They were an example one, one from Sung’s(2018) study, and the other from Park & Lee’s(2018) study. The results were discussed into three conclusions. Firstly, the Q- matrix could provide a objective starting point for a whole sequence of remedial instruction; secondly, the more hierarchical a Q- matrix was the more it helped deciding sequence of remedial instruction; thirdly, knowledge states from cognitive diagnosis might contradict the causal relationship assumptions implied by the Q-matrix.

점진적 체중지지와 보행속도 증가 훈련이 뇌졸중 환자의 보행에 미치는 효과

김성훈,최종덕,Kim, Sung-Hoon,Choi, Jong-Duk 대한물리치료학회 2013 대한물리치료학회지 Vol.25 No.5

Purpose: The aim of this study was to evaluate the effect of progressive body weight decrease combined with increasing level of overground walking speed training for patients with chronic stroke. Methods: Eighteen subjects with chronic stroke were composed of the control group (5% body weight support combined with increasing speed training) and the experimental group (progressive body weight decrease with increasing speed training); three sets, three times per week over a period of four weeks. Results: Significant differences in terms of comfortable gait speed (CGS) and the rate of change of CGS were observed between the control and experimental groups (p<0.05). However, no significant difference in the dynamic gait index was observed between the control and experimental groups (p>0.05). A significant difference in the 6 minute walking test (6MWT) was observed for the experimental group, and a significant difference in the rate of change for the 6MWT was observed between the control and experimental groups (p<0.05). Conclusion: The progressive body weight decrease combined with increasing in level of overground walking speed training may be a better and more effective method for community walking and reintegration.

발효 , 세포배양 , 생물공정 잔나비 걸상 버섯 자실체 및 균사체의 생리활성 탐색

김성훈,이주노,김선희,오세종,안상욱,이진하,박영식,정을권,이현용 ( Sung Hoon Kim,Ju No Lee,Sun Hee Kim,Se Jong Oh,Sang Wook An,Jin Ha Lee,Young Shik Park,Eul Kwon Chung,Hyeon Yong Lee ) 한국미생물생명공학회 1998 한국미생물·생명공학회지 Vol.26 No.4

The effect of captafol on the hematological value, erythrocyte membrane and plasma biochemical value in vitro

김성훈,황화선,차신우,한상섭,노정구,Kim, Sung-hoon,Hwang, Hwa-seon,Cha, Shin-woo,Han, Sang-seop,Roh, Jung-koo The Korean Society of Veterinary Science 1993 大韓獸醫學會誌 Vol.33 No.3

Captafol은 phthalimide계통의 항진균제로써 difolatan이란 상품명으로 개발되어 과일, 채소의 탄저병 및 원목 등의 곰팡이 구제에 널리 사용되고 있는 것으로 국내에서도 두번째로 많이 생산, 사용되는 품목이나 독성실험에 관한 보고는 거의 없는 상태이다. 이에 본 연구에서는 captafol 자체에 대한 독성기전의 일부를 확인하고자 동물체내에서 일어나는 여러가지 대사작용이 배제된 시험관내에서 실험을 수행하였다. 랫트 혈액을 후대정맥으로 부터 채혈하여 4개의 시험관에 $2.5m{\ell}$씩 분주한 후 captafol 농도가 $0.1{\times}10^{-4}M$, $1{\times}10^{-3}M$, $1{\times}10^{-2}M$이 되도록 한 ethanol용액 $25{\mu}{\ell}$씩 넣고 $CO_2$ incubator에서 2시간 동안 부드럽게 혼합하여 혈액학적 성상 및 적혈구 취약성 시험을 수행하였다. 또한 원심분리하여 얻어진 혈장에서 생화학적 성상 등을 조사한 결과 다음과 같은 성적을 얻었다. 1. 적혈구수 및 hematocrit치는 captafol 투여량에 비례적으로 유의한(p<0.05) 감소를 나타내었다. 2. 적혈구막 취약성 실험에서 $1{\times}10^{-3}M$ 및 $1{\times}10^{-2}M$ 농도에서 대조군에 비하여 유의한(p<0.01) 증가를 나타내었다. 3. 혈장내 potassium이온의 농도는 captafol의 투여량에 비례적으로 증가하였다. 4. Total bilirubin 및 creatine kinase가 대조군에 비하여 각각 증가 및 감소하였다. The effect of captafol on the hematological value, erythrocyte membrane and plasma biochemical value was investigated using blood of SPF Sprague-Dawley male rats in vitro. For the anticoagulations, we used 0.5mg of heparin per $10m{\ell}$ of blood from vena cava. Three con-centrations($0.1{\times}10^{-4}M$, $1{\times}10^{-3}M$ and $1{\times}10^{-2}M$) captafol in ethanol were added to the each $2.5m{\ell}$ blood so that the linal concentration of ethanol was 1%. The blood contained with each concentration of captafol was incubated at $37^{\circ}C$ C for 2 hours under 5% $CO_2$ gas The whole blood and plasma were examined for hematological vaJues, erythrocyte membrane damage and biochemical values, respectively. The results obtained were summarized as follows ; 1. The number of RBC in $1{\times}10^{-2}M$ group and the concentration of MCHC in $1{\times}10^{-3}M$ group were significantly (p<0.05) decreased and increased from that of control values, respectively. The percentage of Hct was significantly (p<0.05) decreased with dose-response. 2. Erythrocyte fragility rate of $1{\times}10^{-3}M$ and $1{\times}10^{-2}M$ group were significantly (p<0.01) increased from that control with dose response. 3. Potassium ion level of $1{\times}10^{-2}M$ was significantly (p<0.05) increased from that of control. 4. The concentration of total bilirubin in the $1{\times}10^{-3}M$ and $1{\times}10^{-2}M$ groups were significantly increased from that of control. The enzyme level of creatine kinase in $1{\times}10^{-2}M$ group was significanlty (p<0.05) decreased from control value.

주조 포스트의 유지력에 관한 연구

김성훈,조혜원,진태호,Kim Sung-Hoon,Cho Hye-Won,Jin Tai-Ho 대한치과보철학회 1993 대한치과보철학회지 Vol.31 No.4

The purpose of this study was to evaluate the effects of method for construction of cast post and type of dental cement on the retention of cast post. The wax patterns or Duralay resin patterns were used for construction of cast post. The dental cements used in this study were zinc phosphate cement(Fleck's zinc cement, Mizzy INc., U.S.A.), glass ionomer cement(Fuji I, G-C Co., Japan), and resin cement(Panavia-EX, Kuraray Co., Japan) and the retention of cast post was measured with Instron Universal Test Machine(Instron Engineering Co., U.S.A.). The obtained results were as follows : 1. The tensile bond strength of cast posts made by Duralay resin patterns revealed 39.13(kg) with Panavia-EX, 46.40(kg) with phosphate cement, and 37.78(kg) with glass ionomer cement. 2. The tensile bond strength of cast posts made by wax patterns revealed 39.25(kg) with Panavia, EX, 44.12(kg) with phosphate cement, and 40.23(kg) with glass ionomer cement. 3. The tensile bond strength of cast posts made by Duralay resin patterns or wax patterns were not affected by the type of dental cements(P>0.05).

Cysteine antagonism of captafol induced toxicities in rats 1. Effects on hematological and serum biochemical values

김성훈,Kim, Sung-hoon The Korean Society of Veterinary Science 1995 大韓獸醫學會誌 Vol.35 No.3

과일 및 채소류의 곰팡이 억제제로 개발된 captafol은 인간 및 동물에서의 독성작용은 많이 보고된 바 있으나 이들의 독작용을 완화시키거나 치료할 수 있는 제제에 대한 연구는 별로 알려진 바 없다. captafol에 의한 혈액학 및 혈청 생화화적 변화에 따른 cysteine의 방어 작용을 확인할 목적으로 랫트에 5mg/kg 용량의 captafol을 1회 복강 투여하고 동시에 5, 58, 290 및 580mg/kg cysteine을 각각 투여하고 체중의 변화, 부검소견, 절대장기중량, 혈액학 및 혈청 생화학적 소견을 비교 분석 하였다. captafol 단독 투여군에서는 체중 및 간 장기중량 감소, 복강내 장기들의 섬유소에 의한 유착 및 복수 등이 현저하였으며 적혈구 수, hemoglobin 농도, 혈청 AST의 증가가 인정되었으며 또한 혈청 인지질 함량 감소가 확인되었다. 한편, cysteine 58mg/kg 이상의 용량군에서는 복강내 장기들의 유착 및 복수가 확인되지 않았으며 장기중량, 혈액학 및 혈청 생화학적 소견에서도 정상을 나타내었다. 이 연구 결과는 captafol의 중독증상에 대해 cysteine 및 sulfhydryl군을 가진 물질은 그 증세를 감소시키거나 완화시킬 수 있는 효과가 있음을 보여주고 있다. This experiment was carried out to study the preventive effect of cysteine on the toxicities of captafol to the hematological and serum biochemical values. A single dose of captafol(5mg/kg BW, ip) was given to male Sprague-Dawley rats and its toxicities were evalutated by body weitht changes, autopsy findings, absolute organ weight, and hematological and serum biochemical parameters. The single dose of captafol caused significant decreases in body weitht, and absolute liver weight, as-cites, fibrin clot in abdominal cavity, adhesion of liver lobes significant elevation of number of RBC, hemoglobin concentration and serum AST activity, and decreased of serum phospholipid level. Where as cysteine(over 58mg/kg BW) given immediately after captafol appeared to prevent the ascites, fibrin clot in abdominal cavity and liver lobe adhesion. It also prevented the liver and blood, especially RBC toxicites. The results suggest that cysteine and other compounds containing sulfhydryl groups may protect the subjects from captafol-induced toxicity.