흰쥐 해마에서 Norepinephrine 유리에 미치는 Protein Kinase C 의 영향

김도경,이영수,최봉규,Kim, Do-Kyung,Lee, Young-Soo,Choi, Bong-Kyu 대한약리학회 1995 대한약리학잡지 Vol.31 No.2

The effects and interactions of $4{\beta}-phorbol$ 12,13-dibutyrate(PDB) and polymyxin B(PMB) with adenosine on the electrically-evoked norepinephrine (NE) release were studied in the rat hippocampus. Slices from the rat hippocampus were equilibrated with $^3H-noradrenaline$ and the release of the labelled product, $^3H-NE$, which evoked by electrical stimulation$(3\;Hz,\;2\;ms,\;5\;VCm^{-1},\;rectangular\;pulses)$ was measured. PDB$(0.3{\sim}10\;{\mu}M)$, a selective protein kinase C(PKC) activator, increased the evoked NE release in a dose related fashion while increasing the basal rate of release. And the effects of $1\;{\mu}M$ PDB were significantly inhibited by $0.3\;{\mu}M$ tetrodotoxin(TTX) pretreatment or $Ca^{++}-free$ medium. $PMB(0.03{\sim}1\;mg)$, a specific PKC inhibitor, decreased the NE release in a dose dependent manner while increasing the basal rate of release. Adenosine $(1{\sim}10\;{\mu}M)$ decreased the NE release without changing the basal rate of release, and this effect was significantly inhibited by 8-cyclopentyl-1,3-dipropylxanthine$(2\;{\mu}M)$, a selective $A_1-receptor$ antagonist, treatment. Also, adenosine effects were significantly inhibited by PDB-and PMB-pretreatment. These results suggest that the PKC plays a role in the NE release in the rat hippocampus and might be participated in a post-receptor mechanism of the $A_1-adenosine$ receptor.

SHS법에 의한 Ti-Si계 세라믹스의 합성 및 소결체의 특성에 관한 연구

김도경,박성,조덕호,조건,이형복,Kim, Do-Kyung,Park, Sung,Cho, Duk-Ho,Cho, Kurn,Lee, Hyung-Bock 한국세라믹학회 1994 한국세라믹학회지 Vol.31 No.3

Intermetallic Ti-Si system ceramics were synthesized from stochiometric mixtures of titanium and silicone powders in vacuum by Self-propagating High-temperature Synthesis(SHS). In each cases of Ti5Si3, Ti5Si4 and TiSi, and TiSi2 synthesis, 20wt% product dilution, direct ignition and SHS chemical furnace method were employed. The combustion modes, which were observed during the synthesis process by using the high speed camera, of Ti5Si3, Ti5Si4, TiSi, and TiSi2 exhibit spin, osciallatory, steady-state, and spin combustion, respectively. With increasing Ti/Si molar ratio an decrease of combustion velocities was found. From the results on the measurement of the flexural strength, the specimen hot pressed at 135$0^{\circ}C$ for 30 min using synthesized Ti5Si4 powders showed the highest flexural strength at 215 MPa.

SHS화학로에 의한 SiC의 합성

김도경,박성,조건,이형복,Kim, Do-Kyung,Park, Sung,Cho, Kurn,Lee, Hyung-Bock 한국세라믹학회 1994 한국세라믹학회지 Vol.31 No.11

Ultra-fine $\beta$-SiC powders were fabricated by self-propagating high temperature synthesis process (SHS) using chemical furnace. The dependences of the C powders with different surface areas, the molar ratios of C/Si, the weight ratios of chemical fuel content, and pellet diameter-size on synthesis were investigated. Compositional and structural characterization of these powders was carried out by scanning electron micrograph and X-ray diffraction. The $\beta$-SiC powders which had C/Si mole ratio=1.05, 3 times chemical fuel contents, and pellet diameter=20 mm were optimum for synthesis efficiency. By optimizing process-variables, it is possible to fabricate $\beta$-SiC powders which have little secondary phases ($\alpha$-SiC).

아급성 육아종성 갑상샘염의 세침흡인 세포소견 -아급성 육아종성 갑상샘염 10예의 임상-세포소견 및 면역세포염색 소견 분석-

김도경,표주연,박종필,김연화,정우희,손은주,홍순원,Kim, Do-Kyung,Pyo, Ju-Yeon,Park, Jong-Pil,Jin, Lian-Hua,Jeong, Woo-Hee,Son, Eun-Ju,Hong, Soon-Won The Korean Society for Cytopathology 2008 대한세포병리학회지 Vol.19 No.1

Although subacute granulomatous thyroiditis(SGT) is usually diagnosed clinically, papillary carcinoma or other thyroid conditions must be considered in the differential diagnosis. We retrospectively reviewed the clinical and fine-needle aspiration(FNA) cytologic findings seen in 10 SGT cases to decide what are the most reliable cytologic findings and the most helpful molecular tools for reaching a confident cytologic diagnosis. The most representative smear slides were retrieved to perform immunocytochemistry for cytokeratin 19(CK19) and Ret protein. Five papillary carcinomas(PTCs) were included as controls. The constant and typical cytologic findings of SGT were multinucleated giant cells(MGCs) (100%), epithelioid granulomas(90%), an inflammatory dirty background(90%) and plump transformed follicular cells(80%) without fire-flare cells, oncocytic cells or transformed lymphocytes. The immunoreactivities for CK19(37.5%) and Ret(10%) of the follicular cells of SGT were less than those(CK19 and Ret:100%) of PTC. CK19 immunoreactivity of the MGCs was seen in only one case of PTC. There was no significant difference between CK19 and Ret immunocytochemical staining for the MGCs of both SGT and PTC. The results of this study demonstrate that the cytological diagnosis of SGT can be improved by employing a combination of the typical and constant diagnostic cytological features and immunocytochemical results.

포항 R&BD기관의 집적과 지역에 대한 영향

김도경(Do-Kyung Kim) 대한지리학회 2023 대한지리학회 학술대회논문집 Vol.2023 No.06

전방십자인대 재 재건술 후 슬관절 근력의 비교: 전방십자인대 최초 재건술과의 비교

김도경 ( Do Kyung Kim ),박원하 ( Won Hah Park ) 대한스포츠의학회 2016 대한스포츠의학회지 Vol.34 No.1

The purpose of this study was to assess the knee strength and anterior laxity in between primary and revision anterior cruciate ligament (ACL) reconstruction. It was a cross-sectional study; 28 patients who had undergone revision ACL reconstruction and the another 30 patients who had primary ACL reconstruction once were compared each other which they all had been operated during the same period of season with the same technique in the same hospital. The isokinetic equipment was used to measure the main outcome for knee strength, and KT-2000 arthrometer was used for measuring anterior drawer test in 12 months post-reconstruction surgery. In the anterior drawer test, we found out that there were significantly differences between primary and revision ACL reconstruction groups (1.5±1.1 mm vs. 2.6±1.5 mm, p=0.001). There was no significant difference in the deficits of knee extensor and flexor muscle strength between primary and revision ACL reconstruction. Revision reconstruction groups have more laxity in anterior drawer than primary reconstruction groups. After revision ACL, the deficits of knee extensor and knee flexor muscle strength have no significantly difference from primary ACL reconstruction.

마인추(馬寅初) 비판과 상징을 통한 지식 통제

김도경 ( Do Kyung Kim ) 현대중국학회 2013 現代中國硏究 Vol.14 No.2

이 이 글은 마인추(馬寅初)가 어떻게 사회적 상징이 될 수 있었고, 그리고 어떻게 그 상징이 인구 조절 논의를 장시간 억제할 수 있었는지를 설명한다. 1950년대 그에 대한 비판은 두 차례 있었는데, 이것은 그가 어떠한 방해도 없이 자신의 의견을 제시할 수 있었다는 사실과 밀접하게 관련되어 있다. 중국 공산당이 마인추의 글을 허용하였던 것은 그의 인구 조절론을 보급하기 위했던 것이 아니라, 그의 지식이 얼마나 잘못되었는지를 보여주기 위한 것이었다. 일단 마인추가 거짓 인구론의 상징이 되자, 중국 사회에는 ``금지된 영역(禁區)``이 형성되었고, 마인추라는 상징을 통해 중국 사회는 무엇이 사회주의 인구론인지를 알 수 있었다. 인구 조절론이 장시간 ``금지된 영역``으로 남을 수 있었다는 사실은 중국 공산당이 지식의 관리와 통제에서 물질적이고 강압적인 방법만을 사용한 것이 아니라, 상징을 통해 문화적 구조를 만드는 방식도 사용하였음을 알려준다. This article describes how Ma-Yinchu(馬寅初) has been a symbol, and how the symbol could suppress the discourses of population control. In the 1950s, he was criticized twice, and it was mainly involved with the fact that he was able to post his opinion without interruption. The Communist Party allowed Ma-Yinchu to publish his papers in order not to disseminate his knowledge of population control, but to show how false his knowledge was. Once Ma-Yinchu was a symbol of untruth, ``forbidden area(禁區)`` was created in China. The symbol of Ma-Yinchu showed what is socialist knowledge of population control to Chinese citizens, and this is called as symbolic way of controlling knowledge by the author. The Communist Party not only controlled the material conditions of knowledge production and knowledge circulation, but also invented a symbolic way of controlling knowledge, and this made the discourse of population discourse a ``forbidden area`` for a long time.

코리아 이미지를 이용한 환경시설물 디자인 개발 연구

김도경(Kim Do-Kyung) 한국디자인학회 2006 디자인학연구 Vol.19 No.4

사람 암세포주들에서 아미노산 수송체 LAT1 mRNA 발현과 아미노산 L - Leucine 수송의 상관관계

김도경(Do Kyung Kim),송수근(Soo Keun Song),김인진(In Jin Kim),국중기(Joong-Ki Kook) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.9

사람의 여러 암세포주들에서 아미노산 수송체 LAT1 및 그 보조인자 4F2hc mRNA의 발현과 LAT1의 대표적 기질인 L-leucine 수송을 조사하여 이들 사이의 상관관계를 밝히기 위해, 암세포주들에서 northern blot analysis 및 uptake 실험 등을 시행하여 다음과 같은 결과를 얻었다. 조사한 26 종류의 사람 암 유래 세포주들에서 LAT1 mRNA의 뚜렷한 발현을 확인할 수 있었으나 그 발현양에는 차이가 있었다. 조사한 26종류의 사람 암 유래 세포주들에서 4F2hc mRNA의 뚜렷한 발현을 확인할 수 있었으며, LAT1의 경우에서와 같이 발현하는 정도가 조금씩 다름을 확인할 수 있었다. 사람의 암 유래 세포주들에서 아미노산 transporter에 의한 L-[¹⁴C]leucine 수송을 확인할 수 있었다. 사람의 암세포주들에서 LAT1 mRNA의 발현정도가 높을수록 L-[¹⁴C]leucine의 수송능력이 증가함을 알 수 있었다. 사람의 암세포주들에서 4F2hc mRNA의 발현정도가 높을수록 L-[¹⁴C]leucine 수송 능력이 증가하는 경향은 보였으나, 통계적인 유의성은 없었다. 본 연구의 결과에 의해 사람 암세포주들에서 아미노산 transporter LAT1의 발현과 아미노산 L-leucine의 수송 사이와의 상관관계를 확인할 수 있었으며, 아미노산 transporter LAT1의 특이적 억제에 의한 암세포의 성장 억제에 관한 또 하나의 방향성을 제시할 수 있을 것으로 사료된다. Amino acid transporters play an important role in supplying nutrients to normal and cancer cells for cell proliferation. System L is a major transport system responsible for the Na^+ -independent, large neutral amino acids including several essential amino acids. L-type amino acid transporter 1 (LAT1), an isoform of system L amino acid transporter, is highly expressed presumably to support their continuous growth and proliferation in malignant tumors. In the present study, we have examined the correlation between the expressions of amino acid transporter LAT1 mRNA and its subunit 4F2hc mRNA and the amount of L-leucine transport in various human cancer cell lines. Northern blot analysis have revealed that the 26 human cancer cell lines expressed LAT1 mRNA and 4F2hc mRNA. There were the differences for the levels of LAT1 and 4F2hc mRNA expressions in the 26 human cancer cell lines. The 26 human cancer cell lines transported the L-[¹⁴C]leucine into the cells via amino acid transporter. In the 26 human cancer cell lines, a linear relationship was observed between the expression of amino acid transporter LAT1 mRNA and the amount of L-leucine transport. Little relationship was observed between the expression of 4F2hc mRNA and the amount of L-leucine transport, but the statistical significance of difference was not detected. These results indicate that the 26 human cancer cell lines express LAT1 mRNA and 4F2hc mRNA and there is the correlation between the expression of amino acid transporter LAT1 mRNA and the amount of L-leucine transport. In addition, specific inhibition of LAT1 in cancer cells will be a new rationale for anti-cancer therapy.

모바일 게임 RPG에서 UI에 대한 연구

김도경(Do-Kyung, KIM) 한국컴퓨터정보학회 2016 한국컴퓨터정보학회 학술발표논문집 Vol.24 No.2

본 논문에서는 특정한 모바일 게임 장르인 RPG를 선호하는 유저들의 성향과 매력적인 게임 UI는 무엇인지에 관해 다양한 방법 및 분석한 결과를 제시한다. 모바일 RPG게임에 진입함에 있어 사용자들이 거치는 프로세스를 알아보고, 매력적인 게임 UI는 어떻게 정의하는 가에 대한 것도 제시한다. 게임 UI의 외형이나 기능 등의 요소가 게임을 선택하는 데에 있어 어떠한 영향을 끼치는가에 대해서 알 수 있다. UI와 플레이의 연관성을 분석을 통해 요인을 알아보고, UI가 게임을 진입하는데 있어 사용자에게는 어떤 의미를 가지는가에 대한 것을 알 수 있다.