매체언어 교육에서 문화적 리터러시(cultural literacy)의 구현 방향에 관한 고찰

강민규 강원대학교 국어교육과 2019 語文學報 Vol.39 No.-

Draft genome sequence of Streptomyces sp. P3 isolated from potato scab diseased tubers

강민규,박덕환,Kang, Min Kyu,Park, Duck Hwan The Microbiological Society of Korea 2018 미생물학회지 Vol.54 No.2

Streptomyces sp. P3 균주는 대한민국 강원도 평창의 더뎅이병 이병괴경으로부터 2017년 분리되었다. 이 논문에서는 9,851,971 bp (71.2% G + C 함량)로 구성된 P3 균주의 전체염기서열을 보고한다. 지놈은 8,548개의 코딩서열, 18개의 rRNA 그리고 66개의 tRNA 유전자를 포함하고 있다. 특히 P3 균주는 감자표면과 무종자를 이용한 병원성 검정에서 병원성을 나타내지는 않았지만, 감자 더뎅이병 유발 Streptomyces들이 보유한 병원성 유전자 중 tomA 유전자만이 존재하였다. 따라서 본 논문에 제공되는 전체염기서열은 감자 더뎅이병원세균들의 병원성 획득을 위한 진화단계에서의 이해를 높이기 위한 중요한 단서가 될 것이다. Streptomyces sp. P3 was isolated from potato scab diseased tubers in Pyeongchang, Gangwon-do, Republic of Korea in 2017. Here, we report the draft genome sequences of P3 with 9,851,971 bp size (71.2% GC content) of the chromosome. The genome comprises 8,548 CDS, 18 rRNA and 66 tRNA genes. Although strain P3 did not show pathogenicity both potato tuber assay and radish seedling assay, it possesses tomatinase (tomA) gene among conserved pathogenicity-related genes in well characterized pathogenic Streptomyces. Thus, the genome sequences determined in this study will be useful to understand for pathogenic evolution in Streptomyces species, which already adapted to potato scab pathogens.

내시경 카테터를 통한 반복적 세척으로 치료된 십이지장 게실염

강민규,김국현,김승범,김태년 대한췌장담도학회(구 대한췌담도학회) 2019 대한췌담도학회지 Vol.24 No.3

Effects of candesartan cilexetil and amlodipine orotate on receptor for advanced glycation end products expression in the aortic wall of Otsuka Long-Evans Tokushima Fatty (OETFF) type 2 diabetic rats

강민규,정우백,홍슬기,김옥란,임상현,장기육,승기배 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.4

The receptor for advanced glycation endproducts (RAGE) plays a key role in the development ofvascular inflammation and acceleration of atherosclerosisin type 2 diabetes. We investigated the effect of candesartancilexetil (CDRT) and amlodipine orotate (AMDP)on the expression of RAGE in the aortic walls of OtsukaLong-Evans Tokushima Fatty (OLETF) rats and AGEtreatedendothelial cells. Twenty five-week-old OLETFrats were randomized to 8 week treatments consisting ofCDRT (n = 8), AMDP (n = 8) or saline (control, n = 8). Immunohistochemical and dihydroethidine stainingrevealed reduced RAGE and reactive oxygen species(ROS) signals in rats treated with CDRT or AMDP comparedwith control rats. Both CDRT and AMDP suppressedthe expression of p22phox and p47phox NADPH oxidasesubunits. However, only CDRT significantly reducedexpression of phosphorylated extracellular signal regulatedkinase (pERK)1/2 in the aortic wall of OLETF rats. Inaddition, both drugs reduced RAGE expression and totaland mitochondrial ROS production in the AGE-treatedendothelial cells. Both ARBs and CCBs reduced RAGEexpression in the aortic walls of OLETF rats, which wasattributed to decreased ROS production through inhibitionof NADPH oxidase. In addition, only CDRT reduced aorticexpression of RAGE via suppression of the ERK1/2 pathwayunlike AMDP.

Biodegradable Stereocomplex Polylactide Having Flexible ε-Caprolactone Unit

강민규,정영미,김수현 한국고분자학회 2013 Macromolecular Research Vol.21 No.9

Stereocomplex formation between poly(L-lactide-co-ε-caprolactone) (PLLCL) and poly(D-lactide-co-ε-caprolactone) (PDLCL) is known to be disturbed when their molecular weights increase to >105 g/mol. Here, Strong and biodegradable stereocomplexes of copolymers (S-PLCL) were studied using a supercritical fluid and co-solvent system. Mechanical properties such as tensile strength were ~2.5 times increased due to high degree of stereocomplex than those of PLGA20. The addition of the flexible ε-caprolactone units to the polylactide increased its thermal stability which significantly enhanced 100% degree of stereocomplex after 2nd melt processing through DSC due to the increased mobillity of the polylactide’s chains. The degradation behavior of resulting biodegradable S-PLCL showed slow degradation with increasing CL content in 0.13 M, pH 4.3 phosphate buffers.

Role of Chemotherapy in Stage II Nasopharyngeal Carcinoma Treated with Curative Radiotherapy

강민규,오동렬,조관호,문성호,우홍균,허대석,안용찬,박근칠,박효정,박준수,금기창,차지혜,김준원,김연실,강진형,오영택,김지윤,김성환,김진희,이창걸 대한암학회 2015 Cancer Research and Treatment Vol.47 No.4

Purpose To define the role of neoadjuvant and concurrent chemotherapy in stage II nasopharyngealcarcinoma, we compared the treatment outcomes of patients treated with curative radiotherapywith or without chemotherapy. Materials and MethodsFrom 2004 to 2011, 138 patients with American Joint Committee on Cancer (AJCC) 2002stage II nasopharyngeal carcinoma were treated with curative radiotherapy in 12 hospitalsin South Korea. Treatment methods included radiotherapy alone in 34 patients, neoadjuvantchemotherapy followed by radiotherapy alone in seven, concurrent chemoradiotherapy in80, and neoadjuvant chemotherapy followed by concurrent chemoradiotherapy in 17. Adjuvant chemotherapy was used in 42 patients. Total radiation dose ranged from 64 Gyto 74.2 Gy (median, 70 Gy). ResultsMedian follow-up was 48 months (range, 7 to 97 months) for all patients. At the last followup,13 patients had died and 32 had experienced treatment failure; locoregional failureoccurred in 14, distant failure in 16, and both in two. Five-year locoregional relapse-freesurvival, distant metastasis-free survival, progression-free survival, and overall survival were86.2%, 85.5%, 74.4%, and 88.2%, respectively. Multivariate analyses showed that thesignificant prognostic factors were concurrent chemotherapy and N stage for locoregionalrelapse-free survival, concurrent chemotherapy for progression-free survival, and age andN stage for overall survival. Neither neoadjuvant nor concurrent chemotherapy improveddistant metastasis-free survival. ConclusionConcurrent chemotherapy significantly improved 5-year locoregional relapse-free survivaland progression-free survival in stage II nasopharyngeal carcinoma. However, neoadjuvantchemotherapy failed to improve either

의료기관 수익사업 확대 관련 정책 방향

강민규,Gang, Min-Gyu 대한병원협회 2005 대한병원협회지 Vol.34 No.4

세기조절방사선치료에서 선량율 변화에 따른 선량분포 특성

강민규,김성준,신현수,김성규,Kang, Min-Kyu,Kim, Sung-Joon,Shin, Hyun-Soo,Kim, Sung-Kyu 한국의학물리학회 2010 의학물리 Vol.21 No.2

세기조절방사선치료(intensity modulated radiation therapy, IMRT)는 치료면적을 소조사면으로 나누어 여러 방향에서 방사선이 조사되기 때문에 기존의 치료방법에 비해 많은 MU와 더 긴 치료시간이 요구된다. 통증 및 장애 등으로 인해 장시간 같은 자세를 유지하기 어려운 환자의 경우, 효과적인 치료를 위해서는 선량율을 증가시켜 치료시간을 줄이는 것이 한 방법이다. 본 연구에서는 선량율 변화에 따른 선량 및 선량분포를 측정하고 그 변화를 알아보았다. IMRT 치료계획은 ECLIPSE 시스템(Varian, SomaVision 6.5, USA)을 이용하여, $0^{\circ}$, $72^{\circ}$, $144^{\circ}$, $216^{\circ}$, $288^{\circ}$ 방향의 5문 조사로 계획하였다. 선량율 변화에 따른 선량 및 선량분포 확인을 위해 선량율은 100, 300, 500 MU/min으로 설정하였으며, 선량과 선량분포는 이온함(PTW, TN31014)과 필름(EDR2, Kodak)을 이용하여 각각 측정하였다. 이때 필름 선량계는 아크릴 팬톰에 삽입 후 빔의 조사방향과 나란하게 설치되었고 방사선조사를 위한 선형가속기는 21EX-S (Varian, USA)를 이용하였다. 측정된 필름 선량계는 VXR-16 (Vidar System Corporation)을 이용하여 분석함으로써 선량분포를 확인하였다. 선량율이 증가할수록 CTV를 포함하는 100% 선량분포의 면적이 거의 선형적으로 감소함을 보였다. As radiation is irradiated from various directions in intensity modulated radiation therapy (IMRT), longer treatment time than conventional treatment method is taken. In case of the patients who have problem to keep same posture for long time because of pain and injury, reducing treatment time through increased dose rate is a way for effective treatment. This study measured and found out the variation of dose and dose distribution in accordance with dose rate variation. IMRT treatment plan was set up to investigate from 5 directions - $0^{\circ}$, $72^{\circ}$, $144^{\circ}$, $216^{\circ}$, $288^{\circ}$ - using ECLIPSE system (Varian, SomaVision 6.5, USA). To confirm dose and dose rate in accordance with dose rate variation, dose rate was set up as 100, 300, 500 MU/min, and dose and dose distribution were measured using ionization chamber (PTW, TN31014) and film dosimeter (EDR2, Kodak). At this time, film dosimeter was inserted into acrylic phantom, then installed to run parallel with beam's irradiating direction, 21EX-S (Varian, USA) was utilized as linear accelerator for irradiation. The measured film dosimeter was analyzed using VXR-16 (Vidar System Corporation) to confirm dose distribution.

Intraoperative Radiotherapy for Locally Advanced Rectal Cancer

강민규,김명세,김재황 대한대장항문학회 2010 Annals of Coloproctolgy Vol.26 No.4

Purpose: Although intraoperative radiotherapy (IORT) is known to be a method that can reduce local recurrence in locally advanced colorectal cancer, it is not widely used. The aim of this study was to report our experience with IORT for locally advanced rectal cancer. Methods: From 1991 to 1994, nine patients with locally advanced rectal cancer received IORT. External beam radiotherapy was given postoperatively in five patients and preoperatively in three. Seven patients received chemotherapy. IORT was done with 6-MeV or 9-MeV electrons, and 12 Gy was irradiated at the tumor bed. The median follow-up period was 84 months (range, 15 to 208 months). Results: The median age of patients was 51 years (range, 42 to 73 years). All patients had advanced clinical T-stage (cT3/4)cancer. The overall and the disease-free survival rates were 66.7% and 66.7% at 5 years, respectively. One patient developed a local recurrence near the anastomosis site, which was out of the IORT field. Four patients died before the last follow-up;three from distant metastasis and one from secondary primary cancer. Adverse effects related to IORT did not occur. Conclusion: Although the number of patients was small in this study, IORT is thought to be safe and effective in reducing local recurrence in locally advanced rectal cancer. However, the role of IORT should be refined in the era of preoperative radio-chemotherapy followed by total mesorectal excision.

집중조명 : 전북 군산시 ; 군산시의 지역경제 현황과 활성화 방안

강민규 한국지방행정연구원 2000 지방포럼 Vol.5 No.-