RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

素問·繆刺論에 對한 硏究(Ⅱ)

柳太植,鄭憲瑩,琴坰樹,朴炅 대한한의진단학회 1998 大韓韓醫診斷學會誌 Vol.2 No.2

The Scripture, the research on The MuJa Ron(○刺論) of the So Moon(素問), is described in the MuJa(○刺) of the Kap U1 Kyung(甲乙經). Also it is written in MuJa of Tae So(太素) volume 23,. And Wang(王○) interpreted it in volume 18 chapter 63. In ChimJaRyu(鍼刺類) of RyuKyung(類經) volume 20, it is introduced. And it is mentioned in the 2nd chapter of ShinKyoJung(新校正). In MuJa(○刺), Mu(#) means crossing left and right. Since this paper is describing the differences between MuJa(○刺) and KeoJa(巨刺), and it is also mentioning about carrying out the idea into practice, it is named MuJa(○刺). This paper, the theory of MuJa(○刺), can be divided into three properties in content. Chapter Ⅰ. deals with the common points and differences comparing with KeoJa(巨刺). Chapter Ⅱ. treats the idea that since RakMaek(絡脈) which is invaded by pathogenic factors are different, the states of the disorder are appeared differently so the place, method, atid number and order of acupuncture should be different. In chapter Ⅲ. it is said that in the state of disease of Kyung(經), KeoJa(巨刺) should be applied and in the state of the disease of Rak(絡), MuJa(○刺) should be used. treating oppositThe metabiosis of pathogenic factors flows from the skin and bodyhair to SonRak(孫絡), SonRak(孫絡) to RakMaek(絡脈), RakMaek(絡脈) to internal organs in general. In this situation KeoJa(巨刺) could be used in general. But if pathogenic factors flows irregularly, overflowing from one RakMaek(絡脈) to another place rather than internal organs, MuJa(○刺) could be applied. Therefore in the acupuncture treatment, the theory of MuJa(○刺) which implies side of the troubled places rather than the site itself is very important. But MuJa Ron(○刺論) is too difficult to interpret since is written in old Chinese, the writer of this paper has researched to prove the exact meaning of the MuJa(○刺).

思考技能 訓練프로그램의 開發 및 適用에 關한 硏究 : 衝動性 減少를 中心으로

朴鎭玹,朴敬玉,申東魯,羅東晉,李永植 全北大學校 學生生活硏究所 1994 學生生活硏究 Vol.21 No.-

Many of college students have the cognitive deficits, one of which is the impulsiveness, in the process through which they solve their problems in the daily life. This study attemted to develop the program for the purpose of improving college students' thinking skills. Also, the program was intended to encourage the college students to reduce their impulsiveness, and to help them maintain the rational reasoning solving the complex of problems. For this purpose, researchers examined the difficulties which college students had in the process of their thinking, focussed on their impulsiveness in particular, based on the findings of preceding works related to the thinking skills, including some of programs for improving the thinking skills and intelligence. As a result, a program was developed to improve the thinking skills of college students in order to reduce their impulsiveness. This program was composed of two parts, each of which included five units. Part One, titled "Human Life and the Limited Rationality", was focused on training college students with metacognition or cognitive style. In this part, the trainees explored those phases passed on the human problem solving and the sources of impulsiveness. This part included five units: (1) Introduction; (2) Journey in the universe of learning; (3) Uncertainty and impulsiveness; (4) Human problem solving; and (5) Human information processing and limited rationality. Part Two, titled "Resolution of the Limited Rationality and Intellectual Limitations of Human Being", was focused on training college students with cognitive strategies. In this part, the trainees searched for the general cognitive strategies with which they would learn each unit, and used it to resolve the limited rationality of human being and thus to solve the problems more efficiently. This part included five units: (1) Search for strategies to resolve the intellectual limitations of human being; (2) Utility of the external representations; (3) Application of strategic knowledge of inferences; (4) Resolution of the inferential baises; and (5) Evaluation and termination. A pilot study attempted to verify the effectiveness of the training program. Subjects were 118 sophomores of a national university in Chon-buk. They were assigned to an experimental group(N=56) and a control group(N=62) to test the effectiveness of the training program. The experimantal group received 12 sessions of training instruction for 50 to 60 minutes each week. The control group was given no intervention. After training sessions, they were given a cognitive style (impulsiveness) scale as well as a reasoning test, and also checked a questionnaire which asked the participants to express their opinions of the training program. The findings of the study might be summarized as follows: 1. The program seemed to contribute posively to change participants' cognitive style, particularly to reduce their dysfunctional impulsiveness. 2. The program seemed to contribute substantially to improve participants' performance of reasoning tasks. 3. Many of participants (about tow-third) of the participants reported to have a novel feel- ing on the program, to perceive the program different from the existing curriculum, and also to take some benefits from the program. However, this study suggested several recommandations as follow: 1. It is necessary to verify the effectiveness of the program on the basis of a valid design of experiment. 2. It is necessary to take a effort to extend the breadth and depth of the program, including a discussion of the functional impulsiveness. 3. It is necessary to establish the reinforcement schedule in order to encourage the participant to engage in the program actively.

정신분열증 환자의 말초임파구에서 Fluorodeoxyuridine에 의해 유도되는 Fragile Sites

박경란,김원식 충남대학교 의과대학 지역사회의학연구소 1996 충남의대잡지 Vol.23 No.2

The expression frequency of FUdR-induced fragile sites was examined in schizo phrenia patients to determine whether this parameter could be used as a marker of genetic susceptibility in at risk individuals. No difference was found in expression frequency between the schizophrenia patients and a group of normal individuals. This indicates that the expression frequency of fluorodeoxyuridine induced fragile sites is not a suitable marker for assessing genetic susecptibility in schizophrenia.

한국에서 한 일간지 신문기사 검색을 통한 IMF 전과 후의 자살 양상 비교

박상학,김경환,김학렬,김상훈,표경식,조용래 조선대학교 부설 의학연구소 2000 The Medical Journal of Chosun University Vol.25 No.1

Objectives : Under an IMF bailout program, our country's financial situation forced people to change many things suddenly. Therefore, this study was attempted to search the aspects of changes of suicide attempts in order to find out how the financial crisis affects people's suicide attempts, the extreme repertoire for psychological change Method : November 21, 1997, the day issued the application of IMF bailout program, is the basic date for comparing the changes, on a certain newspaper(Dong-A daily newspaper). The condition was divided into two, namely before and after the basic date, and the characteristics of suicide were compared between two conditions. Results : The news of suicide increased more after than before the application of IMF situation. (before:105, after:158) Specially, for 30-50 years old, active age in community, the rate of suicide attempts reached twice than that of the others. After the IMF situation, the prominent reason of suicide was the financial crisis. For the type of suicide, the family suicide was remarkably increased after the IMF situation. Conclusion : The frequency of the news of suicide was higher after than before IMF bailout program. The major reason of suicide was the financial crisis. Considering that the social and economic crisis affects people's view of death, psychosocial interventions are necessary for their mental health.

성별판별을 위한 한국인 성인 방패연골의 판별분석

박대균,안의태,고정식,박경호 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

방패연골은 목뿔뼈 바로 아래 위치하며 후두부를 구성하는 연골 가운데 가장 큰 연골이다. 본 연구는 한국인 방패연골의 형태분석을 통해 얻은 결과를 이용하여 성별판별을 할 수 있는지 알아보고자 하였다. 20대이상 한국인에서 109(남자69, 여자 40)개의 방패연골을 대상으로 26가지 항목을 계측하여 통계학적으로 검증하여 다음과 같은 결과를 얻었다. 1. 방패연골너비, 방패연골높이, 방패연골길이, 연골판높이 등은 남자의 계측치가 컸다 (p<0.01). 2. 연골판각, 방패연골아래뿔각, 위방패패임각 등은 여자의 게측치가컸다. (p<0.01). 3. 방패연골길이와 연골판높이 항목을 이용하여 남녀판별함수를 얻었다. 이상의 결과에서 한국인 방패연골은 성별에 따라 차이가 있었으며, 20대 이상 한국인의 경우 방패연골의 형태분석에 의해 성별을 판별할 수 있을 것이다.

BCG를 대량 투여 받은 생쥐 가슴샘 겉질의 미세구조

고정식,안의태,박경호,우성임,한영복,경홍기 대한해부학회 1997 Anatomy & Cell Biology Vol.30 No.3

檢査資料의 活用을 통한 集團指導 活動事例의 效果評價

朴敬玉,李永植,宋在鴻 全北大學校 學生生活硏究所 1992 學生生活硏究 Vol.19 No.-

This study was attempted to introduce the group guidance activity, in which the guide has provided the freshmen with their results of psychological tests and has encouraged to improve their self-understanding, and to examine the effectiveness of that activity. In this study, investigators have highlighted the historical background of the group guidance activity through using the results of psychological tests, and have given and outline of the practice of present activity, including 1) the understanding of human behavior and the functions of psychological testing in the base of the information-processing approach ; 2) the main points in interpreting the results of psychological tests and the implications of individual test for self-understanding ; 3) the general strategies for identifying the problems of individuals and coping with them ; and 4) the introduction to the purpose and activities of the Student Guidance and Counseling Center at Chonbuk National University and the guide to use the Center. And then they have examined the direct and/or indirect effectiveness of that activity. This study has suggested several recommandations for the future activity as follows : 1. It is necessary to examine earnestly the purpose and contents of psychological tests used in the group guidance, so that students may search the self-understanding actively. 2. It is necessary to make an effort in helping the students identify the practical problems, specific to psychological adjustment such as interpersonal relationships, adademic performance, career development, and so on, and cope with them. 3. It is necessary to evaluated continuously the effectiveness of the group guidance activity through using the result of psychological tests, and it is followed by formulating a plan which may result in the improvement of that activity in future.

기능성 Catechol-O-Methyltransferase 유전자 다형성과 한국인 정신분열병의 관련성

박태원,홍영준,박임순,김형섭,최인미,배수영,윤경식,강대희 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.4

연구목적 : Catechol-O-Methyltransferase(COMT)는 카테콜아민 신경전달물질의 불활성화를 담당하는 효소로서, 정신분열병과 관련된 후보 유전자로서 알려져 왔다. 최근, 기능성 COMT유전자 다형성과 정신분열병의 관련성이 제기되었는데, 한국인 정신분열병에서도 이러한 관련성이 있는지를 살피기 위해 본 연구를 시행하였다. 방 법 : 정신분열병으로 진단된 환자군 103명과 성별 및 연령별로 1:1 짝짓기 대조군 사이에 유전자형과 대립유전자의 분포를 비교하였다. 또한, 환자군은 공격적 행동, 가족력, 아동기 발병 여부에 따라 두 군으로 나누어 비교하였다. DNA의 다형성 부의를 중합효소연쇄반응에 의해 증폭한 후 ,NlaⅢ제한효소법과 전기영동을 실시하여 유전자형을 판별하였다. 결 과 : 환자군 전체와 대조군 사이에 유전자형과 대립유전자 빈도의 차이가 없었다. 가족력이 있는 환자군을 대조군과 비교하였을 때는, 대립유전자 L을 포함하는 유전자형과 그렇지 않은 유전자형의 분포에 있어 유의한 차이를 보였다(p=0.02, OR=3.9, 95% Cl=1.1∼14.3). 결 론 : 한국인 정신분열병과 기능성 COMT 유전자 다형성 사이에 유의한 관련성이 없었다. 따라서, 기능성 COMT 유전자 다형성이 한국인 정신분열병의 발병에 영향을 주지 못한다. 그러나, 정신분열병 가족력과 기능성 COMT유전자 다형성의 관련성이 시시돠어, 향후 본 연구에서 나타난 방법론적인 문제점을 보완하고 추가 대상자를 확보한 연구가 필요하다. Objectives : Catechol-O-methyltransferase(COMT) is involved in the degradation of catecholamine meurotransmitters and has been investigated as a candidate gene in schizophrenia. Recently, possible relationship between functional COMT gene polymorphism and schizophrenia has been suggested. To address the possible role of functional COMT gene polymorphism in the predisposition to schizophrenia, we carried out an association study in Korean schizophrenic patients and controls. Methods : One hundred and three Korean inpatients diagnosed as schizophrenia and 103 age and sex matched controls were selected as study subjects. Patients were subgrouped into two groups on the basis of history of aggressive behavior, family history of schizophrenia and related disorders, and age at onset. We determined COMT genotypes using PCR of the relevant region followed by digestion with NlaⅢ and electrophoresis. Results : No significant differences of allele and genotype frequencies were noted between patients and controls. However, when patients were categorized by the presence of family history of schizophrenia and related disorders, patients with family history showed almost 4 fold higher frequency of having COMT L allele containing genotype compared to controls(p=0.02, OR=3.9, 9.5% CI=1.10-14.33). Conclusion : Although our results do not support an association between functional polymorphism of COMT gene and schizophrenia overall, the findings suggest an association between functional COMT gene polymorphism and familial schizophrenia. Further studies with large samples are needed to confirm this association.

토끼 눈물샘에서 Nitric Oxide Synthase의 면역조직화학적 연구

박경호,이석호,고정식,안의태,박대균 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Endogenous nitric oxide(NO) has been known to regulate many physiological and pathological process, especially the salivary secretion and glandular blood flow. However, nitric oxide synthase(NOS) responsible for NO synthesis has not been well studied in rabbit lacrimal gland. The present study was performed to investigate the distribution of nitric oxide synthase isoforms(endothelial, neuronal, and inducible NOS). Immunohistochemistry, using monoclonal mouse anti-endothelial NOS, anti-neuronal NOS, and anti-inducible NOS, was performed in inferior lacrimal of the rabbit. Endothelial NOS(eNOS)-positive immunoreactivities were observed in arterial endothelia, intralobular ducts and interlobular ducts of the inferior lacrimal gland of the rabbit. eNOS-positive immunoreactivities were most prominent in the interlobular and intralobular ducts. Immunoreactivities were well concentrated in cytoplasm of columnar epithelial duct cells. However, weak eNOS-positive immunoreactivity was observed in the intercalated ducts and vascular endothelial cells. Neuronal NOS(nNOS)-positive immunoreactivity was seen in the nerve fibers of inferior lacrimal gland. nNOS-positive immunoreactivy was not detected in duct systems or in acinar cells. Inducible NOS-positive immunoreactivity was not seen. Especially, the expression of the nNOS in the nerves of lacrimal gland suggest that NO may play a role in modulating tear production. These results reveal the presence of eNOS and nNOS in the lacrimal gland of the rabbit, which may be related with regulation of the glandular secretion and blood flow through the gland.