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          • 仙方活命飮 및 구성약물의 세포독성에 관한 실험적 연구

            안현주,지선영,An Hyun-jue,Jee Seon-young 대한한의진단학회 2004 大韓韓醫診斷學會誌 Vol.17 No.1

            The purpose of this research was to investigate cytotoxity of Sunbanhwalmyungeum extract. Cytotoxity was determined by MTT assay method. After tumor cell lines(G361, BI6F10, MDA, A549) transplantation, the extracts of SunBangHwalMyungEum and its composition oriental medicines were administered, cytotoxity was measured by absorbance. The results were obtained as follows. 1. Sunbanhwalmyungeum extract and its composition oriental medicines extracts showed the concentration was higher, the more cytotoxity increased. 2. Both water and ethanol extracts of Sunbanhwalmyungeum showed excellent cytotoxity against G361, B16F10, MDA, A549 and high cytotoxity over 80$\%$ against G361, B16F10, MDA except A549 at the concentration of 1000ppm. 3. In water extract, Rhei Radix et Rhizoma, Gleditsiae Spina, Trichosanthis Radix, Glycyrrhizae Radix, Ledebouriellae Radix showed excellent cytotoxity. In ethanol extract, Gleditsiae Spina, Citri Pericarpium, Trichosanthis Radix, Paeoniae Radix Rubra, Myrrha showed excellent cytotoxity. 4. Rhei Radix et Rhizoma, Gleditsiae Spina, Trichosanthis Radix, Paeoniae Radix Rubra showed high cytotoxity in both water and ethanol extrats. 5. In water extract, Glycyrrhizae Radix, Ledebouriellae Radix, Myrrha showed high cytotoxity against A361, Lonicerae Flos, Olibanum, Fritillariae cirrhosae Bulbus, Paeoniae Radix Rubra, Manitis Squama against B16F10, Paeoniae Radix Rubra, Manitis Squama against MDA, Rhei Radix et Rhizoma, Angelicae gigantis Radix against A549. 6. In ethanol extract, Lonicerae Flos, Trichosanthis Radix showed high cytotoxity against G361, Rhei Radix et Rhizoma, Angelicae gigantis Radix, Gleditsiae Spina, Olibanum, Angelicae dahuricae Radix, Fritillariae cirrhosae Bulbus, Paeoniae Radix Rubra, Glycyrrhizae Radix, Ledebouriellae Radix, Myrrha against B16F10, Rhei Radix et Rhizoma, Manitis Squama against MDA, Citri Pericarpium, Manitis Squama against A549.

          • 노화와 피부노화에 대한 고찰

            남혜정,김윤범,Nam Hae-jeong,Kim Yoon-bum 대한한의진단학회 2004 大韓韓醫診斷學會誌 Vol.17 No.1

            In Oriental medicine, aging is just a natural process like change of seasons. Ancient Oriental people accepted it as a natural thing to be growing older and to die at last. The science of aging has advanced dramatically. In the last 2 decades, advances in genetics and molecular biology have led to extraordinary new understandings in how cells age, how apoptosis programs cells to die, and how neuroendocrinology plays a role in the lifespan of organisms. Today, the matter of primary concern about aging is a cellular and mitochondrial damage of human body induced by reactive oxygen species(ROS). The skin aging can be divided into two areas, intrinsic(chronologic)-aging and photo-aging. There are lots of photo damage about skin aging. The skin is increasingly exposed to ultraviolet(UV) irradiation in life. Therefore, the risk of photo-oxidative damage of the skin induced by reactive oxygen species(ROS) has increased substantially. Nowadays, many people believe that they can stop or at least delay the process of aging. There are lots of treatments that promise to slow the process of aging and the associated ailments. Many of these treatments, for example, exercise, Vit E, Vit C therapy, hormone therapy, restrict diet, are gradually being subjected to clinical trials. But in spite of all efforts, researches and investigations, there is no single method or treatment which is revealed to be truly effective for delaying progress of aging. Every methods insisted on effect for delaying aging process, has its dark side. All we can do is just keeping ourself healthy until the time of death.

          • 레이저를 이용한 피부치료의 한의학 임상활용-색소침착 질환을 중심으로

            윤소원,김민희,윤화정,고우신,Yoon So-won,Kim Min-jui,Yoon Hwa-jung,Ko Woo-shin 대한한의진단학회 2004 大韓韓醫診斷學會誌 Vol.17 No.1

            Phototherapy has perpetual history. we can see an instance of it in Yellow Emperor's Canon of Medicine(黃帝內徑) and Laser therapy is a field of Phototherapy being used frequently in the latest time. We treated patients with hyperpigmentation by laser therapy and experimented it's efficacy and studied the means of clinical application in Korean medicine. We report the capabilities of application of laser therapy, based on the Korean medical theories by using resemblance between laser therapy with kyung-rak(經絡) control technique in the line that connects with the past.

          • 靈樞·本藏에 대한 硏究

            朴炅 대한한의진단학회 1997 大韓韓醫診斷學會誌 Vol.1 No.1

            In Bon Jang Pyun (本藏編). pathological mechanisms are explained on the basis of the so-called "Eum-Yang(陰陽)"theory. Summerizing the contents of the Bon Jang Pyun(本藏編) in reference to the contents of successive generation note, the contents were as follows: The difference of five solid organs and six hollow organs is closely connected with the health and the disease. Main factors of the difference are the correctness, angular measure, hardness. relative generosity, height, size, length etc. of five solid organs and six hollow organs. Through five body constituents(tendon, vessel, muscle, hair and skin, bone) we can guess the condition of five solid organs and six hollow organs.

          • 보중익기탕이 알레르기 비염 유발 白鼠의 과립구 및 조직학적 변화에 미치는 효과

            김종성,심성용,엄유식,남혜정,김경준,Kim Jong-sung,Sim Sung-yong,Um Yu-sik,Nam Hae-jeong,Kim Kyung-jun 대한한의진단학회 2004 大韓韓醫診斷學會誌 Vol.17 No.1

            Allergic rhinitis is an allergic reaction characterized by sneezing, coughing, itchy nose, mouth and throat, congestion and/or nasal discharge. The offending allergens are usually pollens, molds, dust mites and animal allergens. We aimed to observe inhibitory effects of Bojungikgi-tang on incerase of the number of eosinophil and neutrophil, and protective effects of nasal mucosal tissue in the allergic rhinitis. 1. In Inhibitory Effect of Bojungikgi-tang on Increase of the number of Eosinophil in Serum, Bojungikgi-tang showed significant effects.(p〈0.05) 2. In Inhibitory Effect of Bojungikgi-tang on Increase of the number of Neutrophil in Serum, Bojungikgi-tang showed significant effects.(p〈0.05) 3. In histopathologic change of nasal mucosal tissue, Bojungikgi-tang showed significant protective effects. Depending upon above results, it is considered that Bojungikgi-tang has the inhibitory effects on the process of allergic rhinitis and that it could be used in reliving patients of the symptoms caused by allergic rhinitis.

          • 加味防風通聖散의 美白效果에 관한 硏究

            이승은,김혜정,김윤범,Lee Seung-eun,Kim Hae-jeong,Kim Yoon-bum 대한한의진단학회 2004 大韓韓醫診斷學會誌 Vol.17 No.1

            Objective: This study was performed to detennine the depigmenting effects of Kamibongpungtongsung-San. Methods: To determine the depigmenting effects of Kamibangpungtongsung-San. we measured the degree of tyrosinase inhibition, melanin production & cell viability in cultured B16 melanoma cells, UV screen and cytoprotective effects on PC12 cells injured by hydrogen peroxide. Results: Komibangpungtongsung-San did not show inhibitory effects on melanin production in melanoma cells, UV screen and cytoprotective effects on PC12 cells injured by hydrogen peroxide. However it showed mild inhibitory effects on tyrosinase activity. Conclusion : This study shows that Kamibangpungtongsung-San, a generally used prescription for dermatologic diseases, do not have depigmenting effects via tyrosinase inhibition. Therefore, the depigmenting effect and mechanism of depigmentation by Kamibangpungtongsung-San need to be evaluated and investigated in other directions.

          • 굴절교정수술에 대한 follow-up Study Review

            정세연,김윤범,Jung Se-yeon,Kim Yoon-bum 대한한의진단학회 2004 大韓韓醫診斷學會誌 Vol.17 No.2

            Refractive surgeries for myopia are rapidly spreading in our daily lives. In this study Korean and foreign follow-up studies, mainly focused on long-term outcomes of LASIK and LASEK, were analyzed. Examining the evidence of the efficacy, accuracy, stability, complications and patient satisfaction will help refine our approach to refractive surgeries.

          • 數種의 한약이 피부질환과 관련된 균주 6종에 미치는 항균력 및 木香이 염증기전에 미치는 영향

            박수연,김종한,최정화,Park Soo-yeon,Kim Jong-han,Choi Jung-hwa 대한한의진단학회 2004 大韓韓醫診斷學會誌 Vol.17 No.1

            This study was carried out to investigate the anti-microbial of various herbal extracts on six types of bacteria related to skin diseases and effects of Saussurea lappa extract on dermatitis and acne. The results are followed ; 1. In the experiment on P. acnes using paper disk methods, Eugenia caryophyllata extract made 19mm clear zone, Saussurea lappa extract and Morus alba extract made 13mm and MIC of Morus alba extract and Eugenia caryophyllata extract was below 0.01$\%$. 2. In the experiment on S. aureus, extract of Eugenia caryophyllata, Poncirus trifoliata and Rubus coreanus made 9-10mm clear zone. 3. In the experiment on C. xerosis, Sesamum indicum extract made 16mm clear zone and MIC of that was 0.3$\%$. 4. In the experiment on C. albicans, extract of Cinnamomum cassia, Eugenia caryophyllata and Asparagus cochinchinensis made 9-10mm clear zone. 5. In the experiment on P. ovale, Cinnamomum cassia extract made 25mm clear one, MIC of that was 0.05$\%$. 6. In the experiment on T. mentagrophytes, Cinnamomum cassia extract made 26mm clear zone, extract of Eugenia caryophyllata, Rhizoma kaempferiae, Piper longum, Saussurea lappa and Zingiber officinale made 18-22mm clear zone, MIC of all extracts was below 0.02$\%$. 7. Saussurea lappa extract inhibited 85$\%$ and 28$\%$ of NO production at 50㎍/㎖ and 5 ㎍/㎖ 8. Saussurea lappa extract inhibited activity of COX Ⅱ over 50$\%$ at 10㎍/㎖ 9. Saussurea lappa extract had not radical scavenging activity and Saussurea lappa extract didn't inhibit manifestation of IL-1${\beta}$, IL-6 and TNF-${\alpha}$, and activity of 50- reductase I.

          • 악관절장애 환자 치험 2례

            김찬중,김윤범,Kim Chan-jung,Kim Yoon-bum 대한한의진단학회 2004 大韓韓醫診斷學會誌 Vol.17 No.2

            Temporomandibular joint disorder is quite common dental problem. Research has shown that 5 to 15$\%$ of the population have a TMJ disorder requiring some type of treatment. TMJ disorder's symptoms are pains in the jaw and muscles of your face, limitation of opening or closing jaw and noises in jaw joint such as clicking or popping sounds. Two patients with TMJ symptoms visited our clinic. They complained pain, limitation of jaw opening and clicking sound. We treated them by stimulating trigger points on the TMJ muscle with acupuncture and had a good effects. The symptoms is reduced or disappeared.

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