ISOLATION AND CHARACTERIZATION OF MOLECULAR MARKER FOR SEVERAL RACES OF PYRICULARIA ORYZAE USING RANDOM AMPLIFIED POLYMORPHIC DNA

Hong, Sun-Min,Bae, Dong-Won,Kim, Mee-Hyang,Shim, Chang-Ki,Kang, Kyu-Young,Kim, Nam-Soo,Kim, Hee-Kyu Plant Molecular Biology & Biotechnology Research C 1994 Plant molecular biology and biotechnology research Vol.1994 No.

Primer 155(GCTGGCGCTG) was the most useful primer with distinctive polymorphic DNA pattern to distinguish between KI races and KJ races. Primer 173(CAGGCGGCGT) showed polymorphic DNA pattern to distinguish isolates of KI-100 race. The specific DNA fragment of 0.6Kb, generated from 88-62 of KJ-201 race by RAPD with primer 412)TGCGCCGCTG), was promising as a molecular marker to identify the isolate.

담낭염 및 담석의 임상적 연구

배진선,길기선,설지영,김제룡 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.1

A retrospective clinical analysis was performed on 152 patients with cholelithiasis who were admitted and treated surgically at the Department of Surgery of Chungnam National University Hospital from January 1995 through December 1996. The results were as follows. 1. The peak incidence of cholelithiasis was 6th decade (27.6%), followed by 7th decade, and the ratio of male to female was 1:1.3. 2. The duration of symptoms was within 1 month in 44.7% of patients. 3. The most common symptom and sign were RUQ abdominal pain (89.5%) and RUQ abdominal tenderness (58.6%). 4. The positive laboratory findings were eleveated ALT (42.8%), elevated AST (38.2%) and leukocytosis (38.8%). 5. The major associated conditions in cholelithiasis were cholecystitis (59.7%), cholangitis (15. 1%) and pancreatitis (9.2%). 6. The location of stones was most prevalent in the gallbladder (86.1%), followed by in the common bile duct (26.9%), and in the intrahepatic duct (12.5%). 7. The most common operative procedure was cholecystectomy (63.2%), followed by cholecystectomy and T-tube drainage (15.8%). 8. Bile was cultured in 44 cases, and the most frequent microorganism was E. coli (40.9%). 9. The postoperative complication rate was 34 of 152 (22.4%), among which residual stone and wound infection were most frequent. 10. The operative mortality was 0.7% and the cause of death was sepsis.

대사성 산증을 동반한 알코올성 간질환 환자의 단기 예후

배기선,유권,조유경,심기남,정성애,문일환 대한간학회 2004 Clinical and Molecular Hepatology(대한간학회지) Vol.10 No.2

목적: 알코올성 간질환에서의 대사성 산증은 지속적인 과량의 알코올 섭취로 인한 당 신생 합성의 억제와 BOHB/AcAc 비율의 증가, 단기간의 금식으로 인한 저혈당, 인슐린 분비 저하, 유리 지방산과 케톤체 형성의 증가, 구토와 수분 섭취 부족에 의한 심한 탈수에 의해 발생하는 것으로 알려져 있다. 알코올성 케톤산증과 감별할 질환으로는 당뇨병성 케톤산증, 젖산증, 살리실레이트, 메탄올, 에틸렌글리콜 중독 등이 있다. 본 연구는 대사성 산증을 동반한 알코올성 간질환으로 내원하여 입원한 환자들을 대상으로 생존군과 사망군에서 임상적 양상 및 검사 소견들의 차이를 비교하여 단기 예후와 연관 있는 인자들을 알아보고자 하였다. 대상과 방법: 만성 알코올 남용자이면서 대사성 산증을 동반하여 입원한 알코올성 간질환 환자 29명을 생존군(18명)과 사망군(11명)으로 나누어 임상 소견과 검사실 소견을 비교하였다. 결과: 1) 대상 환자군은 연령은 36-65세였으며, 성별은 남자가 26명이었고 여자가 3명이었다. 2) 임상 증상은 의식저하, 복통, 구토, 호흡 곤란, 토혈, 흉통 등 다양하게 나타났으며, 신체검사 소견은 탈수, 황달, 빈혈, 간비대, 복수, 부종 등이었고 두 집단을 비교했을 때 사망군에서 복수가 유의하게 더 많은 빈도로 관찰되었다. 일일 평균 음주량은 사망군에서 유의하게 더 많았고(198 vs. 123, P=0.034) 음주 기간과 총 음주량은 두 군 간에 유의한 차이를 보이지 않았다. 4) 내원시 혈액검사 소견을 비교하였을 때 사망군에서 동맥혈 산염기 차이가 유의하게 낮았고 헤모글로빈, 혈소판도 유의하게 낮았다. 사망군에서 총 비리루빈, 크레아티닌, 무기인, 암모니아가 유의하게 높았고 알부민, 클로라이드가 유의하게 낮았다. 사망군에서 프로트롬빈 시간이 유의하게 연장되었으며 섬유소원은 유의하게 낮았고 D dimer는 유의하게 높았다. 결론: 대사성 산증을 동반한 알코올성 간질환 환자에서 일일 알코올 섭취량을 파악하고 복수의 유무, 동맥혈 산염기 차이, 헤모글로빈, 혈소판, 총 빌리루빈, 알부민, 크레아티닌, 무기인, 클로라이드, 암모니아, 프로트롬빈 시간, 섬유소원, D dimer 등을 검사하는 것이 단기 예후를 예측하는 데 도움이 될 것으로 사료된다. 향후 많은 환자들을 대상으로 하여 예후 관련 인자들에 대한 전향적이고 체계적인 연구가 요망된다. Background/Aims: Alcoholic liver disease with metabolic acidosis may have possible causes such as alcoholic ketoacidosis, diabetic ketoacidosis, lactic acidosis. Salicylate, methanol, and ethylene glycol intoxication should also be considered. The aim of this study was to investigate the short-term prognostic factors in patients with alcoholic liver disease with metabolic acidosis. Methods: Clinical data related to twenty-nine patients with alcoholic liver disease and metabolic acidosis was analysed retrospectively. Patients were divided into two groups according to the outcome (survival or death). Past medical history, and physical, laboratory and radiologic data at admission were compared. Results: The amount of daily alcohol intake differed significantly between the two groups (P=0.034), but duration and total amount of alcohol intake did not differ significantly between the two groups (P=0.128; P=0.360). The presence of ascites differed significantly between two the groups (P=0.019). On laboratory testing, the following differed significantly: base excess (P=0.038), hemoglobin (P=0.019), platelet (P=0.040), total bilirubin (P=0.007), albumin (P=0.012), creatinine (P=0.014), phosphorus (P=0.021), chloride (P=0.010), ammonia (P=0.003), prothrombin time (P=0.033), fibrinogen (P=0.011) and D-dimer (P=0.024). Review of the medical history of the patients showed diabetes (10/29), cirrhosis (10/29), and hepatocellular carcinoma (1/29). Combined conditions at admission were sepsis (8/29), pneumonia (7/29), acute renal failure (6/29), rhabdomyolysis (5/29), gastrointestinal hemorrhage (4/29), acute pancreatitis (3/29), acute respiratory distress syndrome (2/29), and acute myocardial infarction (1/29). Conclusions: The amount of daily alcohol intake, base excess, hemoglobin, platelet, total bilirubin, albumin, creatinine, phosphorus, chloride, ammonia, prothrombin time, fibrinogen and D-dimer seemed to be useful parameters in predicting short-term prognosis of patients with alcoholic liver disease with metabolic acidosis. Further study is needed to define the significance of these factors. (Korean J Hepatol 2004;10:117-124)

胃癌患者의 臨床的考察 : Clinical Study and Review of Literatures

裵振善,河基正 충남대학교 의과대학 지역사회의학연구소 1981 충남의대잡지 Vol.8 No.2

The stomach cancer is the most common malignant tumor in men and 2nd or 3rd most common malignant tumor in female in Korea. Retrospective clinical study was performed among 103 patients who were admitted under the diagnosis of stomach cancer during the past 5 years and 6 months from Jan. 1976 to June 1981 to the Dept. of Surgery, School of Medicine, Chung-nam National University Hospital. The following results were obtained; 1. Male predominated the female by 3 : 1. 2. The peak incidence of ages was 6th decade mounting to 43.9%, followed by 5th decade. 3. The predominating symptom was epigastric pain or discomfort by 77.7%. 4. The mass was palpated in 43.7% of patients and epigastric tenderness was revealed in 66.0%. 5. Anacidity or hypoacidity was found in 84.4% and only in 2.2%, the acidity was above normal. 6. Through the radiological study, only 3.6% was revealed normal. 7. Through endoscopy, tumor or ulcer was found in 97.4%. 8. Operations were undertaken in 73.8% of patients, among those, subtotal gastrectomy was in 60.5%, total gastrectomy in 6.6%. 9. Adenocarcinoma was predominated histologically in 97.4%. 10. Postoperative complications were found in 6.3%, among them, wound infections were most frequent(25%), and postoperative mortality was 2.6%. 11. Regarding to clinical diagnosis, 9.7% of patients was proved false positive.

L1210 세포에 대한 약용 식물의 세포독성 검색

배기환,민병선,도동선,김남수,양기종,안병준 충남대학교 약학대학 의약품개발연구소 1992 藥學論文集 Vol.8 No.-

For the research of cytotoxic natural products, 50 medicinal plants were extracted with benzene and methanol, separately, and screened against L1210 cells. From the results(Table I), 6 samples showed cytotoxicity both in benzene and methanol extracts of 17 samples in benzene extracts and 3 samples in methanol extracts, respectively. Generally, the cytotoxicity exhibited high frequency (34%) in benzene extract but low frequency in methanol extract(6%), it meant that active cytotoxic components had less polarity. ED_50 values less than 10 ㎍/㎖ were observed in 17 medicinal plants.

항암화학요법 치료시 환자에서 발생하는 오심 및 구토에서의 정맥주사용 및 경구용 Tropisetron의 제 4상 연구

배진선,김장용,장일성,윤완희,노승무,홍기훈 최신의학사 1997 最新醫學 Vol.40 No.7

은행잎 플라보놀배당체에 대한 셀루라제류의 영향

배기환,민병선,백흠영,안병준 충남대학교 암연구소 1991 癌共同硏究所 硏究誌 Vol.1 No.1

The extractability and stability of ginkgoflavonolglycosides under presence of several cellulase preparations were investigated. The enzymes used were macerosin, cellulase C and cellulase NC. The content variation of the glycosides was measured with HPLC method, using caffeic acid as an internal standard. The methanol extract of ginkgo leaf, containing the total flavonolglyco-sides of 4.46%, was used for the content comparison. By extraction with the enzymes, each or mixed, the peak levels of all the glycosides began to decrease after 1 or 2 hours. After 24 hour extraction, most of the glycosides were degraded to minor components. The flavonolglycosides in ginkgo leaf were also hydrolysed simply by the water extraction. After 24 hour extraction with water at 40°C, the peak levels of major glycosides were distinctly decreased. Rutin was hydrolysed by enzyme treatment or by ginkgo leaf itself. As a result, it was concluded that the commercially available cellulases and the ginkgo leaf itself contain the activities of β-glycosidase and α-rhamnosi-dase. Kaempferol-3-0-(6' -0-p-coumaroylglucosyl)-rhamnoside and four other ginkgo flavonolglycosides were not hydrolysed under the same condition."

복지거제를 위한 자원봉사 활성화 방안

문선화,류기형,박병현,배화숙 부산대학교 사회복지연구소 2000 社會福祉硏究 Vol.10 No.-

영양 장애 환자에 대한 지질 영양 수액제 "Trive-1000^(R)"의 효과에 대한 연구

손기섭,배진선,장일성,노승무,박인근,김지연 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.2

The association between malnutrition and poor hospital course in surgical patients is well recognized, although it may not involve the causation. When the period of NPO is needed more than several weeks, adequate caloric intake is difficult to maintain, and nutritional status often worsens especially after the operation. Trive-1000^(R) is a ready to use industrial ternary mixture. It fulfills standard requirements for TPN and has a advantage to decrease the cost because of time saving in preparation by the pharmacist and cost saving in disposables(transter sets, bags, filters). It improves safety by less risk of contamination and of error from handling and labelling. A prospective study was undertaken to evaluate the efficacy of Trive-1000^(R) in 34 malnourished patients after the major operation and the results were summarized as follows ; 1) The hemoglobin was not changed and AST, ALT, and bilirubin were increased and BUN and alkaline phosphatase were slightly increased without significance. 2) Mild to moderate toxicities including fever and chill, hepatopathy, thrombophlebitis and hyperglycemia. The infusion was discontinued in one of thrombophlebitis and one of hepatopathy. There results suggest that Trive-1000^(R) is relatively effective and safe TPN agents but requires the large scaled prospective randomized study.

대장직장암 환자의 술전 및 술후 혈청 CEA측정의 의의

손기섭,배진선,김인구,장일성,윤완희 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.2

This report is a clinical study of the significance of preoperative and postoperative serial check of plasma CEA levels in 36 cases of colorectal cancer who were admitted and treated at the Chungnam National University Hospital from Mar. 1987 to Oct. 1988 and the results were as follows: 1. The positive rate of preoperative CEA level(over 5ng/ml) in colorectal cancer was 58.3% In resection group, positive rate and mean CEA level were 53.6% and 7.6ng/ml respective and in unresection group they were 75% and 36.7ng/ml respectively (p<0.05). 2. The proportion of patients with abnormal CEA level(over 5ng/ml)was 33.3% in Dukes B, 46.2% in Dukes' C and 69.2% in Dukes'D. 3. The proportion of patients with abnormal CEA level(over 5ng/ml) was 25% in wel1.diffe rentiated type, 60.9%in moderately differentiated type and 37.5 % in poorly differentiated type. 4. The recurrence rate after curative resection in preoperative normal CEA(0~2.5ng/ml) group was presumed 0% in borderline(2.6~ 5ng/ml) group was 33.3%, in high(over 5ng/ml) group was 16.7% 5. In 3 patients with postoperative liver metastasis had the highest frequency of elevated level(over 60ng/ml), whereas those with local recurrence had a lower frequency of elevated level. 6, A patient had second look procedure include segmental resection of local recurrence, buthe was dead of peritoneal caricinomatosis several months later.