몇 가지 항균제가 시험관내에서 내독소와 TNF-α, IL-6 분비에 미치는 영향

최정현,문건웅,김명훈,이동건,박윤희,김상일,김태연,유진홍,김양리,신완식,강문원 대한화학요법학회 1997 대한화학요법학회지 Vol.15 No.2

To evaluate antibiotic-induced endotoxin release(AIER) and its correlation with some cytokines, we measured endotoxin level and tumor necrosis factor alpha(TNF-α) and interleukin6(IL-6) production in mononuclear cells in vitro after exposure of Pseudomonas aeruginosa to antibiotics belonging to different class with two extreme concentrations. The tested concetration of antibiotics were set up according to peak serum level. The low concetration of ceftazidirne and low concentration of imiperiem increased AIER, but high concentration of ceftazideme, high concentration of ciprofloxacin, high concentration of cefoperazone/sulbactam, high concentration of amikacin, and high concentration of meropenem reduced AIER.Interestingly, combined treatment of these antibiotics markedly reduced AIER, But the major cyotkines, TNF-α and IL-6 were not affect by type and concettration of antibiotics, combined treatment of antibiotics, and level of endotoxin released by antiboitics. In this study, we observed AIER was different according to type of antibiotics, concentration of antibiotics, and combination of antibiotics, But AIER had poor correlation with TNF-α and IL-6 in Pseudomonas aeruginosa. It suggests that cytokine release is not solely dependent to endotoxin, but more complex cascade is needed. More invesfigations, such as endotoxin induced cytokine mRNA expression, relationship with penicillin-binding proteins and endotoxin-neutralizing effect of antibiotic itself, must be performed.

NO_x 환원용 Honeycomb촉매의 특성

文一植 순천대학교 공업기술연구소 1993 工業技術硏究所論文集 Vol.7 No.-

This study is concerned with the commercial honeycomb catalyst for the reduction of NO_x by selective catalytic reduction. Three kinds of Honeycomb catalyst, fresh catalyst, half-used catalyst, used catalyst were characterized with X-ray diffraction(XRD), X-ray photoelectron spectroscopy(XPS), Scanning electron microscopy(SEM) and X-ray micro analogy(XMA). Also the catalytic activity of these catalysts was investigated together with the catalytic selectivity.

NO_X제거용 상업용 촉매에 관한 연구

文一植,八島建明 순천대학교 공업기술연구소 1991 工業技術硏究所論文集 Vol.5 No.-

This study is concerned with the commercial honeycomb catalyst for the reduction of NO_x by selective catalytic reduction. The active surface components of this catalyst checked by X-ray micro analyzed were WO_3, V_2O_5 and TiO_2. The catalytic activities about three kinds of catalysts, fresh catalyst, half-used catalyst, used catalyst(catalyst A, B, C, respectively), were measured together with the specific surface area and the pore size distribution. Relative catalytic activity for each catalyst : catalyst A, 1 ; B, 0.62 ; C, 0.40.

Hexane-H_2O계에서 톱밥의 효소 가수분해 반응

문일식,박석규 순천대학교 공업기술연구소 1994 工業技術硏究所論文集 Vol.8 No.-

The enzymatic hydrolysis of sawdust was carried out using cellulase from Aspergillus niger in organic solvent(n-hexane)-water emulsion system and conventional water system. In organic solvent-water system, the water content was found to be a significant factor to the enzymatic hydrolysis and s optimum content was 10 % in total volume of organic solvent-water system. At the above optimum content, a conventional water system. The followings are the optimum condition in organic solvent-water system: pH; 5-6, temp; 30-50℃, reaction time; 96 hrs.

西歸浦市 東部下水處理場 放流水를 利用한 小水力發電

金貴植,文一成 제주대학교 공과대학 첨단기술연구소 2005 尖端技術硏究所論文集 Vol.16 No.1

This study is to investgate the possibility of micro hydroelectric power plants by discharged water from DONGBU SEWAGE TREATMENT PLANT located in SEOGWIPO city, Jejudo. The best location for installing the hydropower plant is at the location of EL(+)2.0, the joint owned surface of the water on the west between discharge flow meter chamber and the surface of the sea water. In calculating the quantity of electric power generation, the flowrate used is 0.157m^(3)/sec, the average 95day water flow for the recent 3years. The effective head is 12.41m between discharge flow meter chamber and the location of hydraulic turbine installation. Therefore, the quantity of electricpower generation is expected to be 14.6kW The monthly quantity of electric generation is 9.46MW and the yearly quantity of electric generation is calculated to be 113.53MW The type of hydraulic turbine to be applied to the hydro power generation is the hydraulic turbine of low head miniflow. Therefore, it is recommended to choose an inline-type hydraulic turbine generator. The recommended capacity is 50kW.

ACF-카트리지를 이용한 수중 페놀의 흡착

이영석,문일식 순천대학교 공업기술연구소 1998 工業技術硏究所論文集 Vol.12 No.-

Adsorption characteristics of phenol in water using batch adsorption and activated Carbon Fiber (ACF)- cartridge were investigated. Adsorption capacities of the ACFs for phenol in water were found to be comparable with those of activated carbons (ACs) which have been widely used for phenol adsorption. In practices, phenol in water was rapidly removed at the first stage by ACF-cartridge. There are neither pressure changes between inlet and outlet nor shape change of cartridge. These studies enabled identification of an activated carbon fibers suitable for use in adsorption of low concentration phenol in water.

프라즈마 噴射層에서의 操作特性

金炳炫,金勝在,文一植,李基完,堀尾正靭 순천대학교 공업기술연구소 1992 工業技術硏究所論文集 Vol.6 No.-

A plasma spouted bed(PSB) was operated to find operating parameters and their effects. Experiments were performed with a laboratory scale PSB(bed diameter : 50㎜, orifice diameter : 4㎜, gas : Ar, plasma condition : DC 100V, 40A). The bed surface was observed from the top window and operating parameters were tested by changing gas flow rate and additional resistance after generating plasma. Also plasma jet was tested with particle loading and without within minimum spouting condition. Plasma power and electric potential increase with decreasing additional resistance and with increasing gas flow rate, but particle loading have no effect on plasma jet.

병원 내 환자에서 분리한 Klebsiella pneumoniae에서 광범위 β-lactamase의 분자생물학적 특성

위성현,김수영,김상일,김양리,유진홍,신완식,강문원 대한병원감염관리학회 2003 의료관련감염관리 Vol.8 No.2

지역사회 획득 급성 신우신장염 여성환자에서 검출된 원인균의 항균제 감수성 및 항균제의 사용

위성헌,최수미,이동건,김수영,김상일,유진홍,신완식,강문원 대한감염학회 2002 감염 Vol.34 No.6

Staphylococcus aureus와 Coagulase-Negative Staphylococcus Species에 대한 Arbekacin의 시험관내 항균력

위성헌,강진한,허동호,이동건,김상일,김양리,최정현,김종현,유진흥,허재균,신완식,강문원 대한감염학회 2001 감염 Vol.33 No.4

Background : Most strains of methicillin-resistant Staphylococcus aureus (MRSA) now exhibit high-level resistance to various antibiotics, such as β -lactam antibiotics, aminoglycosides, macrolides, tetracyclines and quinolones. Recent reports describing the therapeutic failure of vancomycin for MRSA infections have arisen considerable concerns regarding the emergence of MRSA strains, which will require new therapeutic agents. Arbekacin, an aminoglycoside antibiotic, has antibacterial activity against both gram-positive and gram-negative bacteria and is stable in the presence of aminoglycoside inactivating enzymes produced by S. aureus. In this study, we compared the antibacterial activity of arbekacin with those of vancomycin, gentamicin, and amikacin against Staphylococcus aureus (S. aureus) and coagulase-negative staphylococci (CNS). Methods : For a collection of 549 S. aureus and 251 CNS isolates from three Catholic University Hospitals in Korea, minimum inhibitory concentrations (MICs) of arbekacin, vancomycin, amikacin and gentamicin were determined by agar dilution method using Mueller-Hinton agar according to NCCLS (National Committee for Clinical Laboratory Standards, USA)criteria. Results : Among 549 S. aureus isolates, 278 isolates were MRSA and 271 isolates were methicil sensitive S. aureus (MSSA). MIC50 & MIC90 of arbekacin against 549 S. aureus were 0.5 & 1 ㎍/mL, and MIC50 & MIC90 of vancomycin were 1 & 1 ㎍/mL. MIC of arbekacin against 549 S. aureus isolates ranges from 0.03 to 4 ㎍/mL, and MIC of vancomycin against 549 S. aureus ranges from 0.25 to 2 ㎍/mL. MIC90 of amikacin against 549 S. aureus was 32㎍/mL, and that of gentamicin was 128 ㎍/mL. MICs of amikacin and gentamicin were variable, ranging from 0.125 to 256, and otherwise arbekacin and vancomycin revealed relatively narrow range of MICs. MIC90 of arbekacin against 278 MRSA isolates & 271 MSSA were 1 & 0.5 ㎍/mL, and those of vancomycin against MRSA & MSSA were 1 & 1 ㎍/mL. MIC90 of amikacin against 278 MRSA & 271 MSSA isolates were 32 & 4 ㎍/mL, and that of gentamicin against MRSA & MSSA isolates were 128 & 32 ㎍/mL respectively. Among 251 CNS isolates, 122 isolates were MRCNS and 129 were MSCNS. MICSO & MIC90 of arbekacin against 251 CNS isolates were 0.25 & 2 ㎍/mL, and those of vancomycin were 1 & 2 ㎍/mL. MIC of arbekacin against 251 CNS isolates ranges from 0.015 to 32 ㎍/mL, and that of vancomycin isolates ranges from 0.25 to 2 ㎍/mL, MIC90 of arbekacin against 122 MRCNS & 129 MSCNS isolates were 2&0.3 ㎍/ML, and those of vancomycin were 2&s ㎍/ML. MIC90 of amikacin against 251 CNS isolates was 32 ㎍/ML, and that of gentamicin was 128 ㎍/ML for CNS. MIC90 of amikacin against 122 MRCNS & 129 MSCNS isolates were 128 & 8㎍/mL, and those of gentamicin ere 256 & 32 ㎍/mL. Conclusion : Considering above results, arbekacin can be useful agent against most strains of MRSA and MRCMS, which exhibit high-level resistance to amikacin and gentamicin. (Korea J Infect Dis 33:254~260, 2001)