Actinomycetes sp. GF155-2가 생산하는 Pepsin 저해물질의 성질

박석규,성낙계,노종수,김양우,조영숙 한국산업미생물학회 1990 한국미생물·생명공학회지 Vol.18 No.5

Pepsin(8㎎/㎖)에 의한 0.02% casein의 효소적 가수분해반응에서 저해물질의 저해활성은 저해물질농도 20㎍/㎖까지는 비례관계였으며, IC_50은 15㎍/㎖였다. 저해물질의 pH안정성은 pH5∼9 범위내에서 100℃, 10분 가열하였을 때 안정하였고, 열안정성은 pH7.0, 100℃에 20분까지는 100% 저해활성이 나타나 비교적 안정하였다. 효소-저해물질 복합체는 형성되었으며 Lineweaver-Burk plot 상에서 비경쟁적 저해양식이었다. 저해물질은 pepsin, renin의 산성 protease를 강력히 저해하였으며 methanol에 가장 잘 용해되었다. 각종 용매계의 silicagel 60 TLC상에서 R_f치는 0.4∼0.6 범위였고 단일 spot를 나타내었다. IR spectrum 및 정색반응(Rydon-Smith, Biuret)의 결과로부터 peptide성 물질로 간주되며, 융점 및 원소함량은 각각 220∼230℃, C 50.61%, H8.02%, N9.34%였다. When pepsin was used at a concentration of 8 ㎎/㎖ for hydrolysis of 0.02% casein, inhibitory activity of this inhibitor was proportional to a inhibitor concentration of 20 ㎍/㎖, and fifty percent inhibition(IC_50) was observed to be 15 ㎍/㎖. The inhibitor was pH-stable at pH range of 5-9 at 100℃ for 10 minutes and thermo-stable at pH 7.0 at 100℃ to give 100% activity for 20 minutes. The formstion of pepsin-inhibitor complex was confirmed by sephadex G-25 gel filtration and type of inhibition was determined as non-competitive inhibition by Lineweaver-Burk plot. The inhibitor strongly inhibited acid proteases such as pepsin and renin, and it was soluble in methanol very well. On TLC analysis of silicagel 60 using various solvent systems, the inhibitor gave a single spot at Rf range 0.4-0.6. From the result of IR spectrum and color reaction (Rydon-Smith, Biuret), this inhibitor was considered as peptide substance. Melting point and elemental contents were 220-230℃, and C 50.61%-H 8.02%-N9.34%(found), respectively.

PC 통신을 이용한 업무연락망 구축

朴石圭,李喆浩 진주산업대학교 농업기술연구소 1995 農業技術硏究所報 Vol.8 No.-

Nowadays, we have many difficulties in the actual use of the existing communication softwares in school, and they also have some unnecessary parts for school business. Therefore, in this study, we developed our own communication emulator on a realistic basis. For the purpose of rapid information transmission, the system was developed using Turbo-C(Version 2.0) compiler with IBM PC 286 hardware. Some characteristics of the newly-developed system are as follows. Everybody can use freely in the school grounds (without any telephone charges or entrance fee). It is able to be interfaced directly with the original emulators, Chollian, Hitel, etc. It is able to interface home with college, and so on. For the convenience of users, it is composed of full-down menu. For the convenience of interconnection, it can use dial menu.

교육정보화를 위한 ITV의 활용 패러다임 탐색

박석규 울산과학대학 2002 연구논문집 Vol.28 No.2

본 연구는 오락, 정보, 교육을 목표로 하는 ITV의 장점을 살려 교육의 패러다임을 변화시킨다면 교육정보화를 위해 가능할 것이다라는 가능성을 바탕으로 교육정보화를 위한 ITV의 활용 패러다임을 탐색하고자 하였다. 결과를 살펴보면 다음과 같다. 1. ITV를 활용하는 목적은 ITV에 대한 비판적 시청능력의 양성은 물론 ITV를 통해 지식과 정보격차의 해소 그리고 ITV를 실생활에 유용하게 활용하는 능력을 배양해야 할 것이다. 2. ITV 프로그램의 내용은 정보와 오락의 합성어인 인포테인먼트(Infortainment) 프로그램과 교육과 오락의 합성어인 에듀테인먼트(Edutainment) 프로그램으로 통합하는 내용으로 구성, 제작, 방영되어져야 할 것이다. 3. 교육정보화를 위한 ITV의 활용방법은 특기적성 교육활동 활성화, 수준별 수업을 실시하기 위하여 능력별 소집단 학습, 탐구학습, 발표·토론학습, 협력학습 등의 방법으로 활용하여야 할 것이다. 4. 교사들은 ITV 및 정보통신기술의 발달에 따라 아이들의 정보화에 익숙해져 있듯이 정보화교육을 위해 ITV 활용교육에 가장 빠르게 그리고 적극적으로 능동적으로 대처해야 할 것이다. The Purpose of this study is to inquiry on the paradigm of ITV for the Informazation of Education. The results of this study are as following : 1) The aim is to use the ITV program for the Informazation of Education. 2) The contents of the ITV program is the integration progam of the infortainment program and edutainment program etc. 3) The method of the use of ITV program is a small group learning, discovery learning, discuss learning, cooperative learning etc. The role of teacher is quickly, continually, positively, and actively to inquiry on the paradigm of ITV for the Informazation of Education.

TV교육방송 프로그램 활용 시범학교 요구 분석

박석규 울산과학대학 1996 연구논문집 Vol.23 No.1

본 연구는 TV교육방송 프로그램을 활용하여 교육의 질을 높이고자 하는 학교현장에서 방송활용수업을 저해하는 요인 및 문제점을 분석하고, 효율적인 방송활용수업을 위한 교사들의 제언 및 요구를 분석하여 우리 나라의 TV교육방송 프로그램의 제작과 방영 및 운영체제 그리고 학교에서의 학습자의 학습효과뿐만 아니라 정보화교육 등의 교육의 질을 증진시킬 수 있는 방송활용수업에서의 나아가야 할 방향과 시사점을 밝히고자 하는 데 그 목적을 두고 있다. The main research tasks of the present study was two-fold; First, to analyze the present situation and problems in the model schools for instruction using EBS TV program. Second, to analysis suggestions and needs in the model schools for instruction using EBS TV program. Solving these tasks, the purpose of this study is, as a result of it, to find out the directions our nation's instruction using EBS TV program should aim at. For this purpose, EBS TV program is composed to give the first consideration on the programs for the ability of thinking, creativity, problem solving and information processing. And Teachers should understand various studies of the instruction using ETV program, and should find the most suitable instruction using ETV program that is useful to various instructional purposes. And to be the most effective teacher, they should used to every type of the instruction using EBS program. In conclusion, professionals and all persons related to EBS TV program should make efforts to raise a professional expertise.

고온고습으로 저장된 백미의 이화학적 및 취반 특성

박석규,전순실,박미연 순천대학교 기초과학연구소 1994 基礎科學硏究誌 Vol.5 No.-

The quality difference of milled rice stored at a low temperature and optimum humidity(LTH) and a high temperature and humidity (HTH) was determined by the methods of acidity of fat, brown color index, reducing sugar and microflora. Moisture contents of milled rice stored at LTH and HTH for 10 weeks were 14.7% and 19.5%, respectively. Moisture content after 8 weeks of LTH and 4 weeks of HTH exceeded 13% on dry basis, growth-limiting moisture of mold. Brown color index and reducing sugar contents of milled rice stored at HTH were slightly higher than those of LTH. There was a notable difference in acidity of fat between milled and brown rice stored at HTH. The apperance(p<0.05) and texture(p<0.01) of cooked rice after storage for 10 weeks at LTH and HTH were significantly different in sensory evaluation with no significant difference in odor and taste. Mean values of breaking test in rheometer were better in LTH than in HTH for taste score. Growth of mold and bacteria in rice were not increased by storage. Rice weevil exhibited imago in brown rice and milled rice after storage of 5 weeks and HTH. Milled rice stored at LTH for 2 weeks did not absorb Kimchi odor from refrigerated chamber.

Actinomycetes GF 155-2가 생산하는 Pepsin 저해물질의 성질

박석규,조영숙 순천대학교 기초과학연구소 1990 基礎科學硏究誌 Vol.1 No.-

When pepsin was used at a concentration of 8㎎/㎖ for hydrolysis of 0.02% casein, inhibitory activity of this inhibitor was proportional to a inhibitor concentration of 20㎍/㎖, and fifty percent inhibition(IC_50)was observed to be 15㎍/㎖. The inhibitor was pH-stable at pH range of 5-9 at 100℃ for 10 minutes and thermo-stable at pH7.0 at 100℃ to give 100% activity for 20 minutes. The formation of pepsin-inhibitor complex was confirmed by Sephadex G-25 gel filtration and type of inhibition was determined as non-competitive inhibition by Lineweaver-Burk plot. The inhibitor strongly inhibited acid proteases such as pepsin and rennin, and it was soluble in methanol very well. On TLC analysis of silicagel 60 using various solvent systems. the inhibitor gave a single spot at Rf range 0.4∼0.6. From the result of IR spectrum and color reaction(Rydon-Smith, Biuret), this inhibitor was considered as peptide substance. Melting point and elemental contents were mp220∼230℃ and 50.61% -H 8.20% -N 9.34%(found), respectively.

식용식물 추출물과 분획물의 향균활성

박석규,박종철,박미연 순천대학교 기초과학연구소 1994 基礎科學硏究誌 Vol.5 No.-

Antimicrobial activities of acetone and methanol extract of 26 esculent plants were investigated for the screening of natural antimicrobial components. By agar diffusion method, the extracts were tested on their microbial inhibition against 5 microorganisms. Acetone extract(Platycodon grandiflorum, Allium fistulosum, Artemisia princeps var. orientalis, Allium monanthum) and methanol extract(Cirsium japonicum var. ussuriense, Mallotus japonicus, Saururus chinensis) showed strong inhibitory effects against tested microorganisms. Acetone and methanol extract showed potent inhibitory effects on the growth of Staph. aureus and B. subtilis, respectively. Acetone and methanol extract showed a similar trend of antibacterial activity against E. coli and Pseu. aeruginosa. Ethylacetate fraction of Cedrela sinensis and Artemisia princeps var. orientalis also showed a microbial inhibition against most of the bacteria.

백일주 양조중 맛성분의 변화

박석규,박필숙,손봉수,문주석 순천대학교 기초과학연구소 1993 基礎科學硏究誌 Vol.4 No.-

Changes in taste components of Backilju, an old Korean cleraed rice wine, were investigated. During brewing, pH and titratable acidity of mash were decreased or increased in the range of 3.41→3.81 and 8.62→6.74, respectively. The final product of Backilju filtrate, fermented for 100 days, contained 0.36% reducing sugar, 2.68% total sugar, 606.6 ㎎% total acid, 2.5 ㎎% volatile acid and 22.9㎎ solid/ml extract. Major volatile components analized by GC/MS methods were 3-methyl-1-butanol acetate, 4-decanol and dimethyl disulfide. Calcium(20.26ppm) was the most abundant, followed by magnesium(3.19ppm) and zinc(1.87ppm). Nucleotides and their related compounds were inosine(0.12→0.44 mM/ml), ADP(0.16→0.33 mM/ml) and hypoxanthine(0.33→0.04 mM/ml). Compared with other Korean traditional Yakju, Backilju contained more total acid and mineral but less sugar and volatile acid.

아동기(진로인식기) 진로교육에 있어서의 TV의 영향과 방향 탐색

박석규 울산과학대학 2001 연구논문집 Vol.27 No.2

본 연구에서 아동기(진로인식기) 진로교육에 있어서의 TV의 영향과 방향을 탐색하기 위하여 아동기의 진로발달이론과 진로교육, 아동기 진로교육에 있어서의 TV의 영향에 대해 고찰하였다. 그리고 TV를 통한 진로교육의 문제점과 방향 탐색하기 위한 TV를 통한 진로교육 실시상의 문제점과 진로교육을 위한 TV프로그램의 편성 방향과 TV를 통한진로교육에서 교사의 역할과 기능을 탐색하였다. 그 결과를 살펴보면 다음과 같다. 첫째, 진로교육의 접근 방법이 유아기부터 평생동안 단계적으로 실시되어야 하며, 진로교육이 계획적, 체계적으로 실시되어야 할 것이다. 둘째, 아동이 TV를 시청하는 방법에 따라 아동의 진로발달학습의 차이점을 연구하여 아동의 진로발달과 TV를 통한 아동의 진로교육의 가능성을 찾아야 할 것이다. 셋째, TV를 통하여 아동의 진로발달에 도움을 주는 풍부한 정보를 제공하여야 하며, TV를 진로발달과 진로교육을 위한 효율적인 하나의 방법으로 활용해야 할 것이다. 넷째, 진로교육을 위한 TV프로그램을 제작ㆍ방영ㆍ활용하여야 할 것이며, 교사, 학부모, 지역사회를 위한 TV프로그램 개발도 필요할 것이다. 다섯째, 아동을 위한 TV프로그램을 제작할 경우에는 아동의 인지발달 특성을 고려하여야 한다. 여섯째, 진로교육에 대한 TV편성시간을 지속적으로 제작ㆍ방송해야 할 것이며, 교사들은 TV를 통한 진로교육의 개념, 내용과 방법에 대하여 바르게 이해하여야 할 것이다. 일곱째, 교사들은 TV를 통한 진로교육을 아동이 학습하고 준비할 수 있도록 지도해야 할 것이며, 자료의 개발 및 진로교육을 실시할 수 있는 효율적인 방법을 찾아야 할 것이다. The Purpose of this study is to offer the effects and new perspectives of TV for child career education. To achieve the object, this study reviews contents as follows ; 1) theory on the development of career, 2) child career education, 3) the problems of TV for career education, 4) the effects TV for child career education, 5) the new perspectives of TV for child career education, 6) the organization of TV programs for child career education 7) the role of teacher for child career education through TV.

전통 콩식품의 발효기법에 따른 기능성 증진

박석규 한국생명과학회 2001 한국생명과학회 학술발표회 Vol.32 No.-