배추 시스테인 단백질 분해 효소 억제 유전자 BCPI-1의 발현과 종자 발아 및 유묘 생장과의 연관성

홍준기,이은영,김정률,양경애,최영주,정우식,김호일,윤대진,이상열,조무제,임체오 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Phytocystatins are protein inhibitors of cysteine proteinases of the papain family that have been identified in both monocot and dicot plants. A cDNA encoding a phytocystatin, BCPI-1 (Brassica Cysteine Proteinase Inhibitor-1) has been isolated from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds. Here, we tested whether BCPI-1 transcription is regulated by hormones, and could be involved in regulating cysteine proteinases during and after germination. BCPI-1 was sensitive to exogenous GA_(3) and ABA, which are important factors controlling seed germination, suggesting that the expression of BCPI-1 is hormonally regulated. We introduced a recombinant plasmid containing the full-length BCPI-1 cDNA under the control of cauliflower mosaic virus 35S promoter into rice embryogenic calli using the particle delivery method, and regenerated a number of transgenic rice plants. Constitutively over-expressed BCPI-1 caused changes in overall plant growth and development, including reduced germination and seedling growth. These data support the role of the BCPI-1 in the regulation of endogenous proteinases during both seed germination and subsequent seedling development. Phylocystatins은 papain계열의 cysteine 단백질 분해 효소 활성을 특이적으로 억제하는 억제자로 다양한 식물 종으로부터 분리되었다. 본고에서는, 배추 화아 cDNA library로부터 분리된 phytocystatin인 BCPI-1(Brassica Cysteine Proteinase Inhibitor-1)의 생체 내 기능에 대해 연구하였다. 먼저, BCPI-1 전사체는 발아 조절에 중요한 영향을 미치는 GA₃와 ABA에 의해 예민하게 증가, 혹은 감소되는 반응을 보임으로써, BCPI-1이 식물 호르몬의 영향을 받으며, 특히 발아나 유묘의 생장 조절에 관련이 있음을 알 수 있었다. Particle bombardment 방법을 통하여 BCPI-1을 벼의 배 형성 세포 내에 도입, 재분화 시켜 형질 전환 벼를 생성하였다. CaMV 35S promoter의 조절에 의해 지속적인 BCPI-1 발현을 보이는 형질 전환 벼의 경우, 발아와 유묘 생장이 현저히 지연되었다. 위의 결과들을 바탕으로, BCPI-1이 식물체 내에서 생성되는 cysteine 계열의 단밸질 분해 효소 활성을 조절하여 종자의 발아와 유묘의 생장에 영향을 미치는 것으로 추측한다.

박병천류 진도북춤의 '맺음기법'연구

홍은주 한국무용연구회 2012 한국무용연구 Vol.30 No.1

'Heung' may be one of most emotions of Korean people. Among Korean folk dances, Jin-do Drum Dance by Park Byoungchun Sect rooted in Sincheong Nongak, embodies the spirit of dancing in accordance to nature as well as the philosophy of rest-in-motion and presents a structure of improving the emotion of ``Heung`` to the peak through the use of three-prolonged rhythm and internal breathing. I evaluate on breathing as qualitative element to explain emotion. Because the ``invisible thing has contents``, I tried to interpret visible ``beauty of form`` composed by invisible and internal ``beauty of emotion`` with breathing. For Korean folk dance, breathing acts as important factor that can make dance exist as original element which provide life force. The breathing structure of ``bond-humor-relax`` in Korean traditional dance through ceaseless breathing of inhalation and exhalation and various application of breathing techniques, lead to emotional flow which is immanent in dance. Accordingly, this study aims to analyze Jin-do Drum Dance by Park Byoungchun Sect with breathing by using the ``Breathing Notation`` created by Im Hakseon. I try to find how the ``bond technique`` in breathing notation play introduction role to start during dance and how intensity and strength of inhalation, exhalation and breathing change according to intensity of bond technique. I also lead to the emotion of dynamic, simple and unadored ``Heung`` of Jin-do Drum Dance through functional performance of the ``bond technique``. '흥'은 우리 민족의 가장 자연스러운 정서 중에 하나 일 것이다. 민속춤 중 신청농악에 근원을 둔 박병천류 진도북춤은 자연 순응의 춤 정신과 정중동의 철학적 의미를 담고 3연음의 리듬과 순환 호흡으로 ``흥``의 정서를 최고로 상승시키는 구조를 지니고 있다. 논자는 호흡이 ``흥``의 정서를 설명할 수 있는 질적 요소로서 가치와 의의를 두고 있다. ``보이지 않는 것에 실이 있다는 것`` 즉, 보여 지는 ``형식의 미``는 보여 지지 않는 내면적 ``심성의 미``에 의해 이뤄짐을 호흡법으로 해석하고자 한다. 우리 춤에 있어 호흡은 생명력을 주는 원천적인 요소로서 춤이 존재 할 수 있게 하는 중요한 요인이다. ``맺고 어르고 푸는`` 우리 춤의 호흡구조는 들숨, 날숨, 멈춤의 끊임없는 호흡작용과 호흡기법의 활용에 따라 다양한 미적현상으로 드러날 것이다. 이에 임학선이 창안한 ``호흡표기법``을 연구방법의 도구로 활용하여 박병천류 진도북춤 호흡을 분석하고자 한다. 호흡표기에 나타난 끝맺음은 춤이 진행될 때 도입부적 역할을 담당하고, 들숨맺음과 날숨맺음의 강도와 길이에 따라 호흡 크기와 세기의 변화를 찾고자한다. 또한, ``맺음기법``의 적용 여부에 따라 에너지 변화 및 생성에 중요한 수단이 될 것임을 규명하고, ``맺음기법``의 기능적 수행을 통하여 진도북춤의 역동적이며 소박하고 질박한 ``흥``의 정서를 유출하고자 한다.

나사형 임플란트 고정체의 길이, 직경, 플랫폼 형태에 따른 임플란트와 주위조직의 응력분포

강지은,정현주,양홍서 전남대학교 치의학연구소 2002 구강과학 Vol.14 No.1

Seven finite element models were constructed in mandible having single screw-type implant fixture connected to the second premolar-shaped superstructure, in order to evaluate how the length, diameter and platform shape of a screw-type fixture influence the stress in the supporting tissue around fixtures. The superstructure were made using UCLA abutment, and connected to the fixture with abutment screw. Each finite element model was varied in terms of length, diameter, and platform shape of the fixture according to the commercial fixture(3i Implant Innovations Inc, Palm Beach Gardens, FL33410, USA) while the superstructure and the mandible with a single fixture placed in it shared no difference in terms of appearance. In each model, 250N of vertical load was placed on the central pit of an occlusal plane and 250N of oblique load placed on the buccal cusp. The stress distribution in the supporting tissue and the other components was analysed using 2-dimensional Finite element analysis and the maximum stress in each reference area was compared. 1. Under lateral loading, the stress was larger in the larger in the abutment/fixture interface, and in the crestal bone, compared to the stress pattern under vertical loading. 2. The amount of stress at the superstructure was similar regardless of the length, diameter and platform shape of a fixture. 3. Around the longer fixture, the stress was decreased at the bone crest and subjacent cancellous bone and increased in the cancellous bone area apical to the fixture. 4. Around the wider fixture, the stress was decreased at the abutment/fixture interface, and the bone crest and increased in the cancellous bone area apical to the fixture. 5. Around the fixture having wider platform, less stress was resulted at the abutnent/fixture interface and the upper part of the cortical bone, compared to the fixture having standard platform. In short, the stress distribution of the supporting tissue was affected by length, diameter, and platform shape of a fixture, and the fixture which was larger in diameter and length could reduce the stress in the supporting tissues at the bone-fixture interface and bone crest area.

흡입물질의 위생학적 연구 : 본드 흡입이 흰쥐의 폐 및 기타 장기의 효소 활성에 미치는 영향 The Effect of Bond Inhalation on the Enzyme Activity of Lung and Other Organs in Rat

윤수홍,박은주,이주영,서혜령 효성여자대학교 부설 한국환경위생연구소 1991 환경위생연구 Vol.1 No.1

Toluene, alcohol및 vinylester류 등을 함유하고 있는 bond를 이용하여 휘발성 물질이 생체에미치는 영향을 관찰하기 위하여 rat에 일정 농도의 bond를 일정 시간 흡입시킨 뒤 각 조직에대하여 효소의 생화학적 시험과 celluloseacetate electrophoresis를 실시하여 다음과 같은 결과를얻었다. 1. Bond 흡입은 시간이 증가함에 따라 혈청, 심장, 신장, 근육, 뇌의 ChE 활성을 억제하였으며특히 폐와 간장의 ChE활성에 가장 큰 영향을 나타내었다. 2. Bond 흡입에 의해 혈청 및 각 조직 중에서 LDH활성이 증가되었으며 특히 폐와 심장,근육에서 그 영향이 현저히 나타났다. 3. Celluloseacetate electrophoresis를 통한 각 조직의 LDH isozyme pattern에는 차이가 나타났으며 bond 흡입에 의해 band의 소멸과 선명도의 감소가 일어 났는데 특히 폐에 대한 독성이가장 현저했으며 근육에 대한 독성도 큰 것으로 나타났다. Acute poisoning with organic solvents and other volatile compou-nds now usually follows deliberate inhalation(volatile substance abuse) or inges-tion of these compounds. The effect of bond inhalation was analyzed for serum, liver, lung, brain,heart, kidney and muscle. And the observations are revealed on rat cholinesterase activity, lactatedeh-ydrogenase activity and electrophoretic pattern of lactatedehydrogenase isozyme. The results are as follows ; 1. The rat cholinesterase activity on serum, heart, muscle, kidney and brain showthe decrease by increasing of inhalation time of bond, in particular the lungand liver cholinesterase activity was greatly affected. 2. Bond inhalation brough out the lactatedehydrogenase activity increased ofthe serum and the tissues and had an important effect especially in both thelung and muscle lactatedehydrogenase activities. 3. Each tissue was found to have a characteristic distribution of lactatedehydro-genase isozymes on celluloseacetate elelctrophoresis and the development ofinhalation time is shown the disappearence and diffusion of band.

유아교육 연구에서의 ‘자연’과 ‘생태’에 대한 의미 고찰

김은주,임홍남 부산대학교 과학교육연구소 2013 교사교육연구 Vol.52 No.1

The purpose of this study is to explore the meanings of the words, ‘Nature’ and ‘Eco(Ecology)’ and to examine their implication on the early childhood education. For this study, we analyzed a total of six academic journals which were published in the past seven years in Korea, and considered the meanings. There are three categories of the meaning of the word ‘Nature’ in E.C.E’s treatise: ‘Natural environment’, ‘Object of conservation of nature’, and ‘life as Panvitalismus’. Also there are three categories of the meaning of the word ‘Ecology’. The meaning of the word ‘Ecology’ can be divided into three categories in E.C.E’s treatise: ‘Ecology as systematic zoology’, ‘Ecology as eco-friendly’, and ‘Eco-Centered’. The study also observed how those meanings affected early childhood education. Most meaning of those words were based on dualism view of nature and they did not consider its relationship among other lives. These views were resulted from following the same Western ideas as before. Therefore, it may be desirable to redefine the meanings of the words ‘Nature’ and ‘Ecology’ based on the Eastern ideas in E.C.E in order to restore Korean traditional values. In addition these movements may be appropriate for Korean children. 최근 유아교육계에서는 자연친화적 교육 및 생태지향적 교육과 관련된 연구들이 활발하게 진행되고 있다.그러나 유아교육 분야에서 ‘자연’과 ‘생태’라는 용어는 분명하게 정의되지 않는 상태에서 현장의 교사나 연구자들에 의해 상황에 따라 다양하게 사용되고 있다. 따라서 유아교육 분야에서 사용하는 자연과 생태의 의미를 명확하게 밝힐 필요가 제기된다. 본 연구에서는 유아교육 연구에서 사용하는 자연과 생태의 의미를 고찰하고, 그 유아교육적 함의를 살펴보고자 하는 목적으로 시도되었다. 이를 위해 우선 동서양 사상에 나타난 자연과 생태의 의미를 살펴보고, 이를 토대로 유아교육 연구에 나타난 자연과 생태의 의미를 고찰해보았다. 그 결과 자연은 물리적 공간으로서의 자연, 환경보호 대상으로서의 자연, 생명으로서의 자연으로 의미화 되고 있고, 생태는 생물학의 한 분야로서의 생태, 자연 친화로서의 생태, 생명운동으로서의 생태로 나타남을 알 수 있었다. 유아교육 연구에 나타난 자연과 생태의 의미가 대부분 서양 사상의 자연관과 생태관에 바탕을 두고 있음을 볼 때, 유아교육 분야에서 사용되는 자연과 생태의 의미를 한국 전통의 동양 사상을 토대로 한 개념으로 재정의해야 할 필요가 있음을 시사하였다.

우리 나라 보건관리대행서비스 평가 연구

하은희,조수헌,김선민,주영수,한상환,하미나,권호장,홍윤철,김창엽 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.1

Group occupational health service programme started in 1990 is one of the measures to cope with limited human and financial resources in occupational health. The programme has expanded rapidly to include 52 institutions, private as well as public, all over the country. In spite of its potential impact on health of employees and practice of occupational health in small and medium sized industries, comprehensive evaluation in terms of quality has not been tried. This study has aims to develop the criteria to assess the quality of newly developed group occupational health service programme, and to investigate the quality of institutions, and finally to develop policies for the quality improvement. 1) Criteria development : By defining occupational health services, in particular for small and medium sized industries, as one of the primary health care, followings are included as core elements of qualitative occupational health programme; accessibility, continuity, comprehensiveness, technical quality, intersectoral collaboration, emphasis on preventive services, community participation, and adequacy. Again each element is divided into five major components of national health system infrastructure developed by the World Health Organization; development of health resources, organized arrangement of resources, delivery of health care, economic support, and management. In turn, each component is categorized into three aspects of quality assessment, structure, process and outcome. Expert panel selected several criteria for each category to evaluate the programme. Criteria were modified according to each group of interviewees, to produce two sets of questionnaire, one for chief operating officer and another for nurses in the institutions, and the chief operating officer and workers in the workplace. 2) Subject : Of all 52 institutions, 25 voluntarily participated in the survey. At individual institution, chief operating officer and practicing nurses were interviewed in depth. After intensive education for interviewees, every interviewees, every interview was performed with standardized guideline and questionnaire. The quality of the 'Group occupational health service programme' was found to be lower than expected. Especially in continuity, comprehensiveness, technical quality, community participation and adequacy, lower quality in structural aspect was commonly identified throughout all the institutions,. Quality in terms of accessibility and continuity highly varied among institutions., To improve quality of the programme, more comprehensive and systematic programme such as accreditation has to be introduced. In addition, human resources, governmental fund and information systems for individual workers are to be developed. As a long range plan, integration of occupational health services into the national health systems and pooling of financial resources and planned allocation should be considered.

Benzo(a)pyrene에 의해 유도된 간기능장해에 미치는 자근 추출액의 영향

윤수홍,박은주,오관현,정영건,권오진 효성여자대학교 부설 한국환경위생연구소 1994 환경위생연구 Vol.4 No.1

민간에서 주로 사용되는 약용식물들 중 간독성의 발현 저해 및 간기능 보호효과가 우수한 약물을 찾아 임상적인 약효를 밝혀보고자 하는 실험 과정으로 탄소화합물의 불완전 연소 및 열분해에 의해 생성되는 간장해 물질인 benzo(a)pyrene 으로 유도한 rats 의 간독성 발현에 미치는 자근 수침액의 예방 및 치료효과를 실험한 결과 자근 수침액의 투여는 B(a)P 투여로 현저하게 증가된 혈청 및 간장의 AST, ALT, LDH, ALP 활성을 유의성있게 감소시켰고, B(a)P투여로 증가한 혈청 total cholesterol 및 phospholipid 함량 역시 감소시킬 수 있었으나 그 효과는 현저하지 않았다. 즉, 자근 수침액의 투여는 B(a)P에 의한 간독성 발현을 유의성 있게 감소시켰으며 그 효과는 전처리가 후처리에 비해 우수하였다. The present study was undertaken to compare the pharmacologicalactivities of cn de Lithospermi Radix reported with the clinical uses in the orientalmedicine. Crude Lithospermi Radix uesd for the treatment of burn, eczema,blister, diuretic, scarlet fever and septicemia as antipyretic, antidotic and antiph-logistics. Therefore we tested the effects of Lithospermi Radix water extracton the liver-protective activities in the rats. The results obtained from enzymeassay, measurement of serum and liver alanine·aspartate aminotransferase(ALT, AST) and lipid composition indicated that Lithospermi Radix water extractshowed significant liver-protective activities against benzo(a)pyrene-induced he-patotoxicity.

Benzo(a)pyrene에 의해 유도된 간기능장해에 미치는 자근 추출액의 영향

윤수홍,박은주,오관현,정영건,권오진 효성여자대학교 부설 한국환경위생연구소 1993 환경위생연구 Vol.3 No.1

민간에서 주로 사용되는 약용식물들 중 간독성 의 발현 저해 및 간기능 보호 효과가 우수한약물을 찾아 임상적인 약효를 밝혀보고자 하는 실험과정으로 탄소화합물의 불완전 연소및 열분해에 의해 생성되는 간장해 물질인 benzo(a)pyrene으로 유도한 rats의 간독성발현에 미치는 자근 수침액의 예방 및 치료 효과를 실험한 결과 자근 수침액의 투여는B(a)P 투여로 현저하게 증가된 혈청 및 간장의 AST, ALT, LDH, ALP 활성을 유의성있게 감소시켰고 B(a)P 투여로 증가한 혈청 total cholestelof 및 phospholipid 함량 역시감소시킬 수 있었으나 그 효과는 현저하지 않았다. 즉, 자근 수침액의 투여는 B(a)P에의한 간독성 발현을 유의성 있게 감소시켰으며 그 효과는 전처리가 후처리에 비해 우수하였다. The present study was undertaken to compare the phamacologicalactivities of crude Lithospermi Radix reported with the clinical uses in the orientalmedicine. Crude Lithospermi Radix uesd for the treatment of burn, eczema,blister, diuretic, scarlet fever and septicemia as antipyretic, antidotic and antiph-logistics. Therefore we tested the effects of Lithospermi Radix water extracton the liver-protective activities in the rats. The results obtained from enzymeassay, measurement of serum and liver alanine aspartate aminotransferase(ALT,AST) and lipid composition indicated that Lithospermi Radix water extract sho-wed significant liver-protective activities against benzo(a)pyrene-induced hepato-toxicity.

Benzo(a)pyrene에 의해 유도된 간기능 장해에 미치는 쑥의 효과

윤수홍,조수열,박은주,김성중 한국환경독성학회 1992 환경독성보건학회지 Vol.7 No.1

Mugwort has been used as a Korean folk medicine in treating liver diseases acting as an analgesics, sedative, diuresis, choleretics. This study was perfomed to evaluate the effect of mugwort extracts on the changes of enzyme activities, lipid accumulation of the serum and liver, when hepatotoxicity was induced by benzo(a)pyrene. The results are as follows: 1. Mugwort water extract administration prevented the increase of serum and liver AST, ALT, LDH, γ-GTP, liver ALP activities and bilirubin content caused by B(a)P injection. 2. The increase of serum and liver ALT, LDH, γ-GTP, serum AST activities and liver bilirubin contents in B(a)P treated group were decreased by mugwort methanol extract treatment. 3. Serum and liver total cholesterol, phospholipid, triglyceride level and serum HDL-cholesterol level were increased by B(a)P treatment. After combined treatment of mugwort water and methanul extracts, these lipid content were significantly decreased. 4. The hepatotropic effect of mugwort water extract and after-treatment against B(a)P induced hepatotoxicity was superior to that of methanol extract and pretreatment.

의류할인점에 대한 소비자태도 연구

박은주,홍금희 신라대학교 자연과학연구소 1999 自然科學論文集 Vol.7 No.-

The purpose of this study is to help companies in building up the marketing strategy for their clothing discount stores by clarifying consumer's attitude to clothing discount stores and finding out consumer's attitude to clothing discount stores for each type of store. As for the method of the research, 313 female consumers who just finished shopping at clothing discount stores were interviewed and questioned. Factorial analysis, correlation analysis, one way analysis of variance, and t-test were used in analyzing the data. The result is as the following. Firstly, the consumer's attitude to clothing discount store was found to be composed of 3 factors : store loyalty, discount price preference, and well-known brand preference. Secondly, domestic national brand clothing discount store and imported casual brand clothing discount store built more favorable attitude from consumers than domestic causal brand did. As a consequence, consumers using clothing discount stores were proved to be people who prefer discount price and, at the same time, have the desire of buying famous brand products. It means that they would like to buy superior image with low cost by scrutinizing their investment opportunity.