Live Media Streaming in VANETs using Traffic Flow

Shuoping Wang,Zhipeng Wang,Jun Liu,Honghao Gao,Meng Kuai 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.1

Multimedia streaming is a trend in VANETs infotainment service with establishment of infrastructure and communication standards. This paper considers the scenario of live multimedia streaming multicast to vehicles. We propose the relay selection scheme to deliver media streaming with stable link to improve QoS (quality of service). Through evaluation, our proposed scheme is able to have video delivered with a satisfying delay and acceptable loss ratio.

Efficiently targeted therapy of glioblastoma xenograft via multifunctional biomimetic nanodrugs

Zhipeng Yao,Xiaochun Jiang,Hong Yao,Yafeng Wu,Fan Zhang,Cheng Wang,Chenxue Qi,Chenhui Zhao,Zeyu Wu,Min Qi,Jia Zhang,Xiaoxiang Cao,Zhichun Wang,Fei Wu,Chengyun Yao,Songqin Liu,Shizhang Ling,Hongping Xi 한국생체재료학회 2022 생체재료학회지 Vol.26 No.4

Background: Glioblastoma multiforme (GBM) is a fatal malignant primary brain tumor in adults. The therapeutic efficacy of chemotherapeutic drugs is limited due to the blood-brain barrier (BBB), poor drug targeting, and short biological half-lives. Multifunctional biomimetic nanodrugs have great potential to overcome these limitations of chemotherapeutic drugs. Methods: We synthesized and characterized a biomimetic nanodrug CMS/PEG-DOX-M. The CMS/PEG-DOX-M effectively and rapidly released DOX in U87 MG cells. Cell proliferation and apoptosis assays were examined by the MTT and TUNEL assays. The penetration of nanodrugs through the BBB and anti-tumor efficacy were investigated in the orthotopic glioblastoma xenograft models. Results: We showed that CMS/PEG-DOX-M inhibited cell proliferation of U87 MG cells and effectively induced cell apoptosis of U87 MG cells. Intracranial antitumor experiments showed that free DOX hardly penetrated the BBB, but CMS/PEG-DOX-M effectively reached the orthotopic ntracranial tumor through the BBB and significantly inhibited tumor growth. Immunofluorescence staining of orthotopic tumor tissue sections confirmed that nanodrugs promoted apoptosis of tumor cells. This study developed a multimodal nanodrug treatment system with the enhanced abilities of tumor-targeting, BBB penetration, and cancer-specific accumulation of chemotherapeutic drugs by combining chemotherapy and photothermal therapy. It can be used as a flexible and effective GBM treatment system and it may also be used for the treatment of other central nervous systems (CNS) tumors and extracranial tumors.

MiR-200c-3p aggravates gastric cell carcinoma via KLF6

Wang Ying,Lu Kaijuan,Li Weibing,Wang Zhigang,Ding Jing,Zhu Zeyu,Li Zhipeng 한국유전학회 2021 Genes & Genomics Vol.43 No.11

Background Gastric cell carcinoma (GCC) is a common and high-incidence malignant gastrointestinal cancer that seriously threatens human life and safety. Evidences suggest that microRNAs (miRNAs) exhibit an essential role in regulating the occurrence and development of GCC, while the efects and possible mechanisms remain to be further explored. Objective This study was designed to explore whether miR-200c-3p exerted its functional role in the growth and metastasis of GCC, and investigate the possible mechanisms. Methods The expression levels of miR-200c-3p in GCC tissues and cell lines were detected by qRT-PCR analysis. The functional role of miR-200c-3p in the viability, proliferation, migration and invasion of GCC cells were evaluated by CCK-8, EdU, wound healing and Transwell assays. In addition, the candidate targets of miR-200c-3p was predicted and confrmed by dual-luciferase reporter assay. Moreover, the relationship between miR-200c-3p and target (Krüppel like factor 6, KLF6) was assessed by qRT-PCR and western blot assays. Besides, the expression levels of KLF6 in GCC cells were determined by qRT-PCR and western blot assays. Furthermore, the role of KLF6 in the viability, proliferation, migration and invasion of GCC cells mediated with miR-200c-3p mimics was evaluated by CCK-8, EdU, wound healing and Transwell assays. Results In the present study, a new tumor promoting function of miR-200c-3p was disclosed in GCC. We found that the expression of miR-200c-3p was obviously increased in clinic GCC tissues and cell lines. In addition, down-regulation of miR-200c-3p suppressed cell viability, proliferation, migration, and invasion in GCC cells. Moreover, KLF6 was verifed as a direct target of miR-200c-3p by binding its 3’-UTR. Additionally, KLF6 was remarkably decreased and was negatively associated with the miR-200c-3p expression in GCC cell lines. Furthermore, over-expression of KLF6 retarded the efects of miR-200c-3p on the growth and metastasis of GCC cell lines. Conclusions MiR-200c-3p potentially played a tumor-promoting role in the occurrence and development of GCC, which may be achieved by targeting KLF6.

An investigation on the dynamics of domain switching of Bi0.5Na0.5TiO3-based ferroelectric ceramics

Zhipeng Gao,Hang Zhang,Yi Liu,Lingfeng Wu,Jia Yang,Tao Zhang,Haiyan Wang,Xuefeng Chen,Genshui Wang,Hongliang He 한국물리학회 2017 Current Applied Physics Vol.17 No.4

The dynamics of domain switching of Bi0.5Na0.5TiO3-based ferroelectric ceramic was investigated. With the electric field increasing, the domain switch experienced three sections of no domain switch region, creep region and flow region. In the creep region, the domain switch is dominated by the thermally activated domain wall movement. In flow region, the domain wall experience the viscous flow motion. For the BNT-BA-Zn ceramic, the activation energy of creep domain switch is much higher than it in flow region. With temperature increasing, the sensitivity of domain wall motion to the frequency is decreasing. This result demonstrated the domain switch of different ferroelectric materials could be quantitatively analyzed and compared based on the simple polarization - current - electric field data.

Canagliflozin Ameliorates Nonalcoholic Fatty Liver Disease by Regulating Lipid Metabolism and Inhibiting Inflammation through Induction of Autophagy

Zhipeng Xu,Wenxin Hu,Bin Wang,Ting Xu,Jianning Wang,Dan Wei 연세대학교의과대학 2022 Yonsei medical journal Vol.63 No.7

Purpose: Nonalcoholic fatty liver disease (NAFLD) is closely associated with metabolic diseases, including obesity and diabetes,and has gradually become the most common cause of chronic liver disease. We investigated the effects of sodium glucose cotrans porter 2 (SGLT2) inhibitor canagliflozin on NAFLD in high-fat diet (HFD)-induced obese mice and possible underlying mecha nisms. Materials and Methods: Male C57BL/6 mice were fed a normal-diet, HFD, or HFD with canagliflozin for 14 weeks. AML-12 hepa tocytes were treated with canagliflozin. Expression of related pathways was assessed. Results: Canagliflozin administration reduced body weight and fat mass, compared with HFD alone. Canagliflozin improved glu cose and lipid metabolic disorders. Compared with HFD-fed mice, liver weight, serum alanine transaminase (ALT) levels, and he patic lipid accumulation were decreased after canagliflozin administration. Additionally, canagliflozin upregulated lipolysis mark ers (CPT1a, ACOX1, and ACADM), downregulated lipogenesis markers (SREBP-1c and FASN), and suppressed the production ofinflammatory cytokines (TNFα, MCP1, IL-1β, and IL-6), consistent with significantly increased LC3 II/I and Atg7 levels in the liverfollowing canagliflozin treatment. In vitro, canagliflozin increased CPT1a, ACOX1, and ACADM expression, decreased SREBP-1cand FASN protein expression, and reduced TNFα, MCP1, IL-1β, and IL-6 mRNA levels in lipid mixture (LM)-induced hepatocytesin a dose-dependent manner. These changes were reversed by 3-MA, an autophagy inhibitor. Conclusion: Our findings suggest that canagliflozin ameliorates the pathogenesis of NAFLD by regulating lipid metabolism andinhibiting inflammation, which may be associated with its promotion of autophagy.

Triptolide downregulates Rac1 and the JAK/STAT3 pathway and inhibits colitis-related colon cancer progression

Zhipeng Wang,Haifeng Jin,Ruodan Xu,Qibing Mei,Daiming Fan 생화학분자생물학회 2009 Experimental and molecular medicine Vol.41 No.10

Triptolide, a diterpenoid triepoxide from the traditional Chinese medicinal herb Tripterygium wilfordii Hook. f., is a potential treatment for autoimmune diseases as well a possible anti-tumor agent. It inhibits proliferation of coloretal cancer cells in vitro and in vivo. In this study, its ability to block progress of colitis to colon cancer, and its molecular mechanism of action are investigated. A mouse model for colitis-induced colorectal cancer was used to test the effect of triptolide on cancer progression. Treatment of mice with triptolide decreased the incidence of colon cancer formation, and increased survival rate. Moreover, triptolide decreased the incidence of tumors in nude mice inoculated with cultured colon cancer cells dose-dependently. In vitro, triptolide inhibited the proliferation, migration and colony formation of colon cancer cells. Secretion of IL6 and levels of JAK1, IL6R and phosphorylated STAT3 were all reduced by triptolide treatment. Triptolide prohibited Rac1 activity and blocked cyclin D1 and CDK4 expression, leading to G1 arrest. Triptolide interrupted the IL6R-JAK/STAT pathway that is crucial for cell proliferation, survival, and inflammation. This suggests that triptolide might be a candidate for prevention of colitis induced colon cancer because it reduces inflammation and prevents tumor formation and development. Triptolide, a diterpenoid triepoxide from the traditional Chinese medicinal herb Tripterygium wilfordii Hook. f., is a potential treatment for autoimmune diseases as well a possible anti-tumor agent. It inhibits proliferation of coloretal cancer cells in vitro and in vivo. In this study, its ability to block progress of colitis to colon cancer, and its molecular mechanism of action are investigated. A mouse model for colitis-induced colorectal cancer was used to test the effect of triptolide on cancer progression. Treatment of mice with triptolide decreased the incidence of colon cancer formation, and increased survival rate. Moreover, triptolide decreased the incidence of tumors in nude mice inoculated with cultured colon cancer cells dose-dependently. In vitro, triptolide inhibited the proliferation, migration and colony formation of colon cancer cells. Secretion of IL6 and levels of JAK1, IL6R and phosphorylated STAT3 were all reduced by triptolide treatment. Triptolide prohibited Rac1 activity and blocked cyclin D1 and CDK4 expression, leading to G1 arrest. Triptolide interrupted the IL6R-JAK/STAT pathway that is crucial for cell proliferation, survival, and inflammation. This suggests that triptolide might be a candidate for prevention of colitis induced colon cancer because it reduces inflammation and prevents tumor formation and development.

Numerical analysis of Poiseuille-Rayleigh-Bénard convection in supercritical carbon dioxide

Wang Zhipeng,Xu Hong,Chen Chong,Hong Gang,Song Zhenguo,Zhang Yaoli 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.9

The supercritical carbon dioxide (SeCO2) Brayton cycle is an important energy conversion technology for the fourth generation of nuclear energy. Since the printed circuit heat exchanger (PCHE) used in the SeCO2 Brayton cycle has narrow channels, Rayleigh-Benard (RB) convection is likely to exist in the tiny channels. However, there are very few studies on RB convection in supercritical fluids. Current research on RB convection mainly focuses on conventional fluids such as water and air that meet the Boussinesq assumption. It is necessary to study non-Boussinesq fluids. PRB convection refers to RB convection that is affected by horizontal incoming flow. In this paper, the computational fluid dynamics simulation method is used to study the PRB convection phenomenon of non-Boussinesq fluid-supercritical carbon dioxide. The result shows that the inlet Reynolds number (Re) of the horizontal incoming flow significantly affects the PRB convection. When the inlet Re remains unchanged, with the increase of Rayleigh number (Ra), the steady-state convective pattern of the fluid layer is shown in order: horizontal flow, local traveling wave, traveling wave convection. If Ra remains unchanged, as the inlet Re increases, three convection patterns of traveling wave convection, local traveling wave, and horizontal flow will appear in sequence. To characterize the relationship between traveling wave convection and horizontal incoming flow, this paper proposes the relationship between critical Reynolds number and relative Rayleigh number (r).

Research of Formal Verification for SQL Operations in Top Level Specification of a Secure Database

Zhipeng Wang,Hong Zhu,Meiyi Xie 보안공학연구지원센터 2012 International Journal of Database Theory and Appli Vol.5 No.3

Changes of projectile attitude and its influence parameters during the process of penetration on the ship plate structure through material point method

Yinan Wang,Zhi Wang,Xiongliang Yao,Nana Yang,Zhipeng Chen 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.2

In this paper, the attitude changes of projectile and its influence parameters during the process of penetration on the ship plate structure are studied by material point method. The method is firstly verified by comparing with experimental results. The numerical results agree well with the experimental results. The failure behavior of the plate is similar to the actual situation, and the failure mode is basically the same. With the change of different parameters, the attitude of projectiles will change differently. The greater the penetration velocity, the weaker the influence of other related parameters on the projectile attitude. When other parameters are not changed, the relative size of the strength of stiffener and the thickness of target plate determines the change of the projectile attitude. Analyze from the perspective of energy and force, and finally projectile attitude can be divided into four types.

Combined Lowering Effects of Rosuvastatin and L. acidophilus on Cholesterol Levels in Rat

( Lijun Wang ),( Baihua Zhou ),( Xue Zhou ),( Yang Wang ),( Hongwei Wang ),( Shengying Jia ),( Zhipeng Zhang ),( Chao Chu ),( Jianjun Mu ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.3

Statins are a class of lipid-lowering drugs commonly used in the prevention of cardiovascular diseases. However, statin therapy presents many limitations, which have led to an increased interest in non-drug therapies, such as probiotics, to improve blood cholesterol levels. Indeed, probiotic strains such as Lactobacillus acidophilus have been found to improve blood lipid profiles, especially in reducing total cholesterol and LDL-C levels. In this study, we established a high-cholesterol rat model and studied the effect of Lactobacillus acidophilus administration alone or in combination with rosuvastatin. We were able to show that Lactobacillus exerts a cholesterol-lowering effect. Additionally, we observed that when administered together, rosuvastin and Lactobacillus exert a combined cholesterol-lowering effect. Altogether, our data advocate for the possibility of establishing probiotics as non-drug supplements for the treatment of hypercholesterolemia.