Molecular identification of DNA barcoding of Leguminous toxic species and quantitative analysis by ELISA kits

Wang Jie,Wang Shuangyu,Sun Fenglin,Liu Chang,Zhao Jinquan,Yu Hongwei,Lv Xiaojing,Liu Ze,Bu Shuhua,Yu Weisen 한국식물생명공학회 2024 Plant biotechnology reports Vol.18 No.2

Some edible Leguminous are toxic when raw, and the Chinese are particularly fond of beans, so Leguminous poisoning is very common in China. Rapid and accurate identification of poisoned species and determination of their toxic components would better assist physicians in treating patients. However, traditional morphology-based identification methods possess many limitations. DNA barcoding technique is a new species identification technique developed in recent years, which is expected to make up for the shortcomings of traditional morphological identification. In this study, a comprehensive evaluation system based on DNA barcoding and ELISA kits was attempted. A total of 30 Leguminous toxic plants were collected, involving 9 genera and 10 species. We used simulated gastric fluid (SGF) to simulate the human gastric environment. Three markers (rbcL, trnH-psbA, and ITS) were amplified and sequenced for all untreated and 15 mock-digested samples. The validity of DNA barcoding for species identification was assessed using the Basic Local Alignment Search Tool (BLAST) method and the tree construction method. The levels of three toxic components (saponin, phytoagglutin and trasylol) were determined in all samples using ELISA kits. The amplification success rate of all three regions was high (rbcL 96.67%, trnH-psbA 100%, and ITS 100%), but the sequencing of the trnH-psbA region was less satisfactory (66.67%), and SGF had a significant impact on the sequencing of the ITS region (After 40 min of SGF treatment, the sequencing success rate decreased by 46.67%). The samples from different species and origins contained different levels of toxic components, and the levels of all three sub- stances decreased significantly after undergoing SGF digestion. After 1 h of SGF treatment, the saponin content decreased to 0–8.60% in untreated content (PHA decreased to 8.62–36.88%, trasylol decreased to 4.70–47.06%). The current results suggest that DNA barcoding has great potential for rapid identification of Leguminous poisoning in clinical settings. Toxins are probably not detectable in the patient for longer periods of poisoning. We recommend DNA barcoding technology as a first step for rapid screening and combined with toxin analysis for clinical diagnosis.

An Adaptive Trust Sampling Method for P2P Traffic Inspection

Hongwei Chen,Dongyang Yu,Chunzhi Wang,Shuping Wang 보안공학연구지원센터(IJGDC) 2013 International Journal of Grid and Distributed Comp Vol.6 No.6

Genomics and LC-MS Reveal Diverse Active Secondary Metabolites in Bacillus amyloliquefaciens WS-8

Hongwei Liu,Yana Wang,Qingxia Yang,Wenya Zhao,Liting Cui,Buqing Wang,Liping Zhang,Huicai Cheng,Shuishan Song,Liping Zhang 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.3

Bacillus amyloliquefaciens is an important plant disease-preventing and growth-promoting microorganism. B. amyloliquefaciens WS-8 can stimulate plant growth and has strong antifungal properties. In this study, we sequenced the complete genome of B. amyloliquefaciens WS-8 by Pacific Biosciences RSII (PacBio) Single Molecule Real-Time (SMRT) sequencing. The genome consists of one chromosome (3,929,787 bp) and no additional plasmids. The main bacteriostatic substances were determined by genome, transcriptome, and mass spectrometry data. We thereby laid a theoretical foundation for the utilization of the strain. By genomic analysis, we identified 19 putative biosynthetic gene clusters for secondary metabolites, most of which are potentially involved in the biosynthesis of numerous bioactive metabolites, including difficidin, fengycin, and surfactin. Furthermore, a potential class II lanthipeptide biosynthetic gene cluster and genes that are involved in auxin biosynthesis were found. Through the analysis of transcriptome data, we found that the key bacteriostatic genes, as predicted in the genome, exhibited different levels of mRNA expression. Through metabolite isolation, purification, and exposure experiments, we found that a variety of metabolites of WS-8 exert an inhibitory effect on the necrotrophic fungus Botrytis cinerea, which causes gray mold; by mass spectrometry, we found that the main substances are mainly iturins and fengycins. Therefore, this strain has the potential to be utilized as an antifungal agent in agriculture.

針對交際意圖的認知語境硏究

wang hongwei 영남중국어문학회 2019 중국어문학 Vol.0 No.80

At present, the study of context is in a flourishing stage. Language scholars and other scholars with contextual related disciplines have studied context from different angles. In general, the linguistic context of linguistic context is still focused on the application, limited to the branch of linguistics, and in the study of the division of various branches of linguistics. The basic theory of context is still very backward, and the context is still lack of a comprehensive, comprehensive, comprehensive study and a understanding of the context. It is still vague, and there has been no significant progress in the basic theoretical research on the nature, form, structure and type of context. At present, contextual research has been paid more and more attention by linguists and scholars, and has become a very important research content in linguistic research. Context research is gradually getting rid of the subsidiary status of other disciplines and is developing towards an independent research direction of linguistics.

Experimental study of the behavior of beam-column connections with expanded beam flanges

Hongwei Ma,Jiwei Wang,Eric M. Lui,Zeqing Wan,Kun Wang 국제구조공학회 2019 Steel and Composite Structures, An International J Vol.31 No.3

This paper describes an experimental study of steel beam-column connections with or without expanded beam flanges with different geometries. The objectives of this study are to elucidate the cyclic behavior of these connections, identify the location of the plastic hinge zone, and provide useful test data for future numerical simulations. Five connection specimens are designed and tested under cyclic load. The test setup consists of a beam and a column connected together by a connection with or without expanded beam flanges. A constant axial force is applied to the column and a time varying point load is applied to the free end of the beam, inducing shear and moment in the connection. Because the only effect to be studied in the present work is the expanded beam flange, the sizes of the beam and column as well as the magnitude of the axial force in the column are kept constant. However, the length, width and shape of the expanded beam flanges are varied. The responses of these connections in terms of their hysteretic behavior, failure modes, stiffness degradation and strain variations are experimentally obtained and discussed. The test results show that while the influence of the expanded beam flanges on hysteretic behavior, stiffness degradation and energy dissipation capacity of the connection is relatively minor, the size of the expanded beam flanges does affect the location of the plastic hinge zone and strain variations in these beam-column joints. Furthermore, in terms of ductility, moment and rotational capacities, all five connections behave well. No weld fracture or premature failure occurs before the formation of a plastic hinge in the beam.

基於缺省邏輯的函數推理

wang hongwei 중국어문학회 2018 中國語文學誌 Vol.0 No.64

The research on Default in linguistic had a long history, and the terminologies used to define this phenomenon have also been verified, but better widely known as Omission. Even not a small number of the researchers ignored the difference and mixed them in usage, but in this research paper, it was found that there had been substantial difference in between. Omission is more Grammarian-based method, while Default is more Pragmatics-based method. In this applied linguistics prevailing era, the research on Omission is no longer focus on the analysis or reasonable explanation of those grammarian rules, weakening the status of Omission syntax. The conveying of information is not only through the grammar but also, or saying in a bigger portion, through the speculation during the real communication and the cognitive context behind it. The Default Function could explain this finding brief and to the point. In this paper it started with a detailed inference from the previous research on the definition of Default Function, adding the prosody component and Paralinguistic Features into “D”, redefining the scope of Paralinguistic Features, so as to revise the function for a better expression of the Default phenomenon. It is known by now, there has not been much research on this topic, and this is also the first paper on the revision and intensification of Default Function in linguistic. Hopefully the research on Default in cognitive context in this paper could make some contribution in opening the black box for further understanding our human’s brain.

The Specific Case Analysis of Biomineralization Induced by Sulfate Reducing Bacteria

( Hongwei Liu ),( Shuang Qin ),( Chaoyang Fu ),( Fei Xiao ),( Deli Wang ),( Xia Han ),( Tianli Wang ),( Hongfang Liu ) 한국부식방식학회(구 한국부식학회) 2017 Corrosion Science and Technology Vol.16 No.6

The effects of sulfate reducing bacteria (SRB) on the corrosion and scaling of the Q235 carbon steel has been investigated in the simulated sewage water and oil field gathering pipelines production water, using scanning electron microscopy (SEM), energy dispersive x-ray spectrometry (EDS), and three-dimensional stereoscopic microscope. Results indicated that the concentration of SRB reached the maximum value on the ninth day in simulated sewage water with a large amount of scaling on the surface of specimen. In oil field gathering pipelines, a large amount of scaling and mineralization of mineral salts and thick deposition of extracellular polymeric substance (EPS) layers were also observed on the surface of specimen. The thickness of biofilm was about 245 μm within 30 days. After adding microbicides, the thickness of corrosion products film was only up to 48 - 106 μm within 30 days, suggesting that SRB could induce biomineralization. Under-deposit corrosion morphology was uniform in the absence of microbicides while local corrosion was observed in the presence of microbicides.

Adaptive guided salp swarm algorithm with velocity clamping mechanism for solving optimization problems

Wang Zongshan,Ding Hongwei,Wang Jie,Hou Peng,Li Aishan,Yang Zhijun,Hu Xiang 한국CDE학회 2022 Journal of computational design and engineering Vol.9 No.6

Salp swarm algorithm (SSA) is a well-established population-based optimizer that exhibits strong exploration ability, but slow convergence and poor exploitation capability. In this paper, an endeavour is made to enhance the performance of the basic SSA. The new upgraded version of SSA named as ‘adaptive strategy-based SSA (ABSSA) algorithm’ is proposed in this paper. First, the exploratory scope and food source navigating commands of SSA are enriched using the inertia weight and boosted global best-guided mechanism. Next, a novel velocity clamping strategy is designed to efficiently stabilize the balance between the exploration and exploitation operations. In addition, an adaptive conversion parameter tactic is designed to modify the position update equation to effectively intensify the local exploitation competency and solution accuracy. The effectiveness of the proposed ABSSA algorithm is verified by a series of problems, including 23 classical benchmark functions, 29 complex optimization problems from CEC 2017, and five engineering design tasks. The experimental results show that the developed ABSSA approach performs significantly better than the standard SSA and other competitors. Moreover, ABSSA is implemented to handle path planning and obstacle avoidance (PPOA) tasks in autonomous mobile robots and compared with some swarm intelligent approach-based path planners. The experimental results indicate that the ABSSA-based PPOA method is a reliable path planning algorithm.

Characterization of Brain Microstructural Abnormalities in High Myopia Patients: A Preliminary Diffusion Kurtosis Imaging Study

Wang Huihui,Wen Hongwei,Li Jing,Chen Qian,Li Shanshan,Wang Yanling,Wang Zhenchang 대한영상의학회 2021 Korean Journal of Radiology Vol.22 No.7

Objective: To evaluate microstructural damage in high myopia (HM) patients using 3T diffusion kurtosis imaging (DKI). Materials and Methods: This prospective study included 30 HM patients and 33 age- and sex-matched healthy controls (HCs) with DKI. Kurtosis parameters including kurtosis fractional anisotropy (FA), mean kurtosis (MK), axial kurtosis (AK), and radial kurtosis (RK) as well as diffusion metrics including FA, mean diffusivity, axial diffusivity (AD), and radial diffusivity derived from DKI were obtained. Group differences in these metrics were compared using tract-based spatial statistics. Partial correlation analysis was used to evaluate correlations between microstructural changes and disease duration. Results: Compared to HCs, HM patients showed significantly reduced AK, RK, MK, and FA and significantly increased AD, predominately in the bilateral corticospinal tract, right inferior longitudinal fasciculus, superior longitudinal fasciculus, inferior fronto-occipital fasciculus, and left thalamus (all p < 0.05, threshold-free cluster enhancement corrected). In addition, DKI-derived kurtosis parameters (AK, RK, and MK) had negative correlations (r = -0.448 to -0.376, all p < 0.05) and diffusion parameter (AD) had positive correlations (r = 0.372 to 0.409, all p < 0.05) with disease duration. Conclusion: HM patients showed microstructural alterations in the brain regions responsible for motor conduction and visionrelated functions. DKI is useful for detecting white matter abnormalities in HM patients, which might be helpful for exploring and monitoring the pathogenesis of the disease.

A Secure Data Transmission Scheme Based on Information Hiding in Wireless Sensor Networks

Baowei Wang,Hongwei Qian,Xingming Sun,Jian Shen,Xiaoyu Xie 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.1

Wireless sensor networks hold the promise of facilitating large-scale and real-time data processing in complex environments, therefore the security is a critical issue that must be resolved. In order to prevent attacks by adversaries with fake identities, we propose a secure data transmission scheme based on information hiding technique. Firstly, one superficial change made to the beacon message of original CTP is to mark the unique identity infor-mation of a sensor node. Secondly, sensitive information is uniformly and randomly embed-ded into the ordinary data by using the space-efficient randomized data structure characteris-tic of Bloom Filter. Experimental results and performance analysis demonstrate that the pro-posed scheme can efficiently detect the malicious node with fake identities through the sensi-tive information. On the other hand, our scheme can protect the sensitive information without affecting the transmission and usability of the ordinary data.