Carbon monoxide prevents TNF-α-induced eNOS downregulation by inhibiting NF-κB-responsive miR-155-5p biogenesis

Choi, Seunghwan,Kim, Joohwan,Kim, Ji-Hee,Lee, Dong-Keon,Park, Wonjin,Park, Minsik,Kim, Suji,Hwang, Jong Yun,Won, Moo-Ho,Choi, Yoon Kyung,Ryoo, Sungwoo,Ha, Kwon-Soo,Kwon, Young-Guen,Kim, Young-Myeong Nature Publishing Group 2017 Experimental and molecular medicine Vol.49 No.11

<P>Heme oxygenase-1-derived carbon monoxide prevents inflammatory vascular disorders. To date, there is no clear evidence that HO-1/CO prevents endothelial dysfunction associated with the downregulation of endothelial NO synthesis in human endothelial cells stimulated with TNF-α. Here, we found that the CO-releasing compound CORM-2 prevented TNF-α-mediated decreases in eNOS expression and NO/cGMP production, without affecting eNOS promoter activity, by maintaining the functional activity of the <I>eNOS</I> mRNA 3′-untranslated region. By contrast, CORM-2 inhibited MIR155HG expression and miR-155-5p biogenesis in TNF-α-stimulated endothelial cells, resulting in recovery of the 3′-UTR activity of <I>eNOS</I> mRNA, a target of miR-155-5p. The beneficial effect of CORM-2 was blocked by an NF-κB inhibitor, a miR-155-5p mimic, a HO-1 inhibitor and siRNA against HO-1, indicating that CO rescues TNF-α-induced eNOS downregulation through NF-κB-responsive miR-155-5p expression via HO-1 induction; similar protective effects of ectopic HO-1 expression and bilirubin were observed in endothelial cells treated with TNF-α. Moreover, heme degradation products, except iron and <I>N</I>-acetylcysteine prevented H<SUB>2</SUB>O<SUB>2</SUB>-mediated miR-155-5p biogenesis and eNOS downregulation. These data demonstrate that CO prevents TNF-α-mediated eNOS downregulation by inhibiting redox-sensitive miR-155-5p biogenesis through a positive forward circuit between CO and HO-1 induction. This circuit may play an important preventive role in inflammatory endothelial dysfunction associated with human vascular diseases.</P>

Protective Effects of Hyperoside against Carbon Tetrachloride-Induced Liver Damage in Mice

Choi, Jun-Ho,Kim, Dong-Wook,Yun, Nari,Choi, Jae-Sue,Islam, Md. Nurul,Kim, Yeong-Shik,Lee, Sun-Mee American Chemical Society and American Society of 2011 Journal of natural products Vol.74 No.5

<P>In this study, the hepatoprotective effects of hyperoside (<B>1</B>), a flavonoid glycoside isolated from <I>Artemisia capillaris</I>, have been examined against carbon tetrachloride (CCl<SUB>4</SUB>)-induced liver injury. Mice were treated intraperitoneally with vehicle or <B>1</B> (50, 100, and 200 mg·kg<SUP>−1</SUP>) 30 min before and 2 h after CCl<SUB>4</SUB> (20 μL·kg<SUP>−1</SUP>) injection. Levels of serum aminotransferases were increased 24 h after CCl<SUB>4</SUB> injection, and these increases were attenuated by <B>1</B>. Histological analysis showed that <B>1</B> prevented portal inflammation, centrizonal necrosis, and Kupffer cell hyperplasia. Lipid peroxidation was increased and hepatic glutathione content was decreased significantly after CCl<SUB>4</SUB> treatment, and these changes were reduced by administration of <B>1</B>. Protein and mRNA expression of tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and heme oxygenase-1 (HO-1) and nuclear protein expression of nuclear factor erythroid 2-related factor 2 (Nrf2) significantly increased after CCl<SUB>4</SUB> injection. Compound <B>1</B> suppressed TNF-α, iNOS, and COX-2 protein and mRNA expression and augmented HO-1 protein and mRNA expression and Nrf2 nuclear protein expression. These results suggest that <B>1</B> has protective effects against CCl<SUB>4</SUB>-induced acute liver injury, and this protection is likely due to enhancement of the antioxidative defense system and suppression of the inflammatory response.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jnprdf/2011/jnprdf.2011.74.issue-5/np200001x/production/images/medium/np-2011-00001x_0006.gif'></P>

Vascular Protective Role of Samul-Tang in HUVECs: Involvement of Nrf2/HO-1 and NO

Choi, Eun Sik,Lee, Yun Jung,Seo, Chang Seob,Yoon, Jung Joo,Han, Byung Hyuk,Park, Min Cheol,Kang, Dae Gill,Lee, Ho Sub Hindawi Publishing Corporation 2016 Evidence-based Complementary and Alternative Medic Vol.2016 No.-

<P>Samul-Tang (Si-Wu-Tang, SMT), composed of four medicinal herbs, is a well-known herbal formula treating hematological disorder or gynecologic disease. However, vascular protective effects of SMT and its molecular mechanisms on the vascular endothelium, known as the central spot of vascular inflammatory process, are not reported. The aim of this study was to investigate vascular protective effects of SMT water extract in human umbilical vein endothelial cells (HUVECs). Water extract of SMT was prepared and identified by HPLC-PDA analysis. Expression of cell adhesion molecules (CAMs) and heme oxygenase-1 (HO-1) and translocation of nuclear factor-kappa B (NF-<I>κ</I>B) and nuclear factor-erythroid 2-related factor 2 (Nrf2) were determined by western blot. Nuclear localization of NF-<I>κ</I>B and Nrf2 was visualized by immunofluorescence and DNA binding activity of NF-<I>κ</I>B was measured. ROS production, HL-60 monocyte adhesion, and intracellular nitric oxide (NO) were also measured using a fluorescent indicator. SMT suppressed NF-<I>κ</I>B translocation and activation as well as expression of CAMs, monocyte adhesion, and ROS production induced by TNF-<I>α</I> in HUVECs. SMT treated HUVECs showed upregulation of HO-1 and NO which are responsible for vascular protective action. Our study suggests that SMT, a traditionally used herbal formula, protects the vascular endothelium from inflammation and might be used as a promising vascular protective drug.</P>

심근 허혈-재관류 백서 모델에서 mitogen-activated protein kinase 활성화에 대한 α-lipoic acid의 효과

김연경,박은미,최준호,윤경호,유남진,이은미,김남호,정진원,김민선,박병림,오석규 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.1

인지/감정 혼합광고평가에 대한 사전지식과 관여도의 조절적 역할

최낙환,김종호,황윤용 조선대학교 경영경제연구소 2002 經營經濟硏究 = Management and economics research Vol.25 No.1

입원한 정신분열병 환자의 종합 검사 중 로샤 검사의 대응 손상 지표(Coping Deficit Index) 수준과 다른 검사들 간의 관계 연구

최윤희,김재환,이영호 가톨릭대학교 사회과학연구소 2005 社會科學硏究 Vol.21 No.-

본 연구는 정신분열병 집단 내에서 로샤 검사의 대응 손상 지표 수준과 다른 검사들의 수행 간의 관계를 통해 한 검사에서 보이는 지표가 다른 검사에서 어떻게 나타나는지 확인하고자 하였다. 정신과 입원 환자 중 정신분열병으로 진단된 환자들을 로샤 검사의 대응 손상 지표 수준에 따라 집단1(CD1=<3)과 집단2(CDI>3)와 같이 두 집단으로 나누었다. 이들을 대상으로 K-WAIS, MMPI, SCL-90-R 검사와 같이 객관적 채점 및 해석이 가능한 변인을 고찰하였다. 연구 결과, K-WAIS 검사의 상식 소검사, 어휘 소검사, 이해 소검사, 공통성 소검사, 언어성 지능 및 전체 지능에서 두 집단 간에 유의미한 차이를 보였다. 그러나 이를 제외한 K-WAIS의 나머지 소검사들과 동작성 지능에서는 차이가 유의미하지 않았으며, MMPI와 SCL-90-R 검사의 소척도들에서도 집단 간에 유의미한 차이가 나타나지 않았다. 끝으로 본 연구의 제한점 및 향후 연구 방향이 논의되었다. The purpose of this study was to explore how the signs of a psychological test could be manifested in the other test. The subjects groups consisted of following: group 1(CDI=<3), group 2(CDI>3). The results are as follows: First, the group 1 showed greater performance than group 2 in the K-WAIS subtests as information, vocabulary, comprehension, similarities, verbal intelligence quotient and full scale intelligence quotient. But there was no significant results on the other subtests. Second, there were no significant differences in the subtests of the MMPI nor the SCL-90-R between the group 1 and group 2. In conclusion, research implications, limitations of the study and suggestions for future studies were discussed.

우리나라 感潮河川 水位資料의 初基分析

崔秉昊,徐景錫,吳允根 成均館大學校 科學技術硏究所 1987 論文集 Vol.38 No.2

우리나라 主要河川의 感潮區間에서의 水位觀測은 1910年代부터 시작되었으나 感潮區間의 潮汐變化를 判別키 위한 이 長期資料의 徹底한 分析은 遂行된 바 없다. 本 硏究에서는 利用可能한 漢江, 錦江感潮區間에서의 5個所(顚類, 金浦大橋, 玉浦, 笠浦, 江景)의 感潮水位資料를 分析하기 위한 初期努力이 試圖되었다. 原記錄曲線으로부터 每時間別로 編輯된 水位資料로부터 統計的 變數들과 潮汐의 調和常敎를 구했는데 檢討結果에 의하면 旣存水位資料들은 不適合한 觀測體系 및 記錄體系 때문에 資料의 質은 극히 不良하였다. Observations of the water levels in the tidal reaches of major rivers in South Korea have been made since 1910s. But these extended records have never been subjected to intensive analysis to identify the tidal variation in the shallow rivers. In the present study, an initial attempt was made to analyze the available tidal data at five gauging stations(Jeonryu, Kimpo Bridge, Okpo, lbpo and Ganggyeong) in the Han River and the Keum River. Statistical parameters and harmonic constants were obtained from edited hourly water levels taken from original water level curves. It was found that existing data were seriously corrupted by improper measurement and recording system.

예비수학교사의 신념에 따른 교수학적 내용지식(PCK)과의 관련성에 관한 연구

고상숙,김은호,문정윤,배지은,정대진 이화여자대학교 교과교육연구소 2011 교과교육학연구 Vol.15 No.4

This study investigated the relationship between PCK (Pedagogical Content Knowledge) and pre-service mathematics teachers' mathematical beliefs, and the effects of PCK on their teaching. The data were collected through pre & post interviews, a questionnaire, and classroom observations of six pre-service mathematics teachers who were taking their field practica in their senior year. The results indicate that the pre-service mathematics teachers' differing beliefs about the nature of mathematics affected their PCK. This study showed the relationship between their beliefs about teaching and the learning of mathematics and PCK. It is recommended that more opportunities for PCK development, as well as for modifications in of their beliefs have to be offered in education programs for pre-service mathematics teachers. 본 연구의 목적은 예비수학교사들의 수학적 신념과 수업에서 발현된 교수학적 내용지식(PCK)의 특징을 알아보고, 이를 통해 수학적 신념에 따른 PCK의 관련성에 대해 분석하는 것이다. 이 연구는 교육실습 중인 예비수학교사 6명을 대상으로 실시한 PCK 설문지, 인터뷰 자료, 수업관찰 자료를 분석하는 질적 연구 방법을 토대로 하였다. 연구결과, 예비수학교사들의 다양한 수학 본질에 대한 신념은 PCK에 서로 다른 영향을 주었으며, 수학 교수-학습에 대한 신념과 PCK사이에는 밀접한 관련성이 있음을 볼 수 있었다. 따라서 신념과 교수학적 내용지식(PCK)을 함께 발달시킬 수 있는 예비교사 연수 및 지원 프로그램의 확충이 필요하다.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

PC U형의 단면을 사용한 포스트텐션된 넓은 보-기둥 내부접합부의 비탄성 거동

최윤철,임재형,문정호,이리형,권기혁 대한건축학회 2005 大韓建築學會論文集 : 構造系 Vol.21 No.1

Post-tensioned precast concrete system(PPS) consists of U-shaped precast wide beams and concrete columns. The continuity of beam-column joint is provided with the topping concrete on the PC shell beam and post-tensioning. The purpose of this paper is to evaluate the response of PPS interior beam-column joint subjected to cyclic lateral loading. To this end, the experimental investigation was performed with three half-scale specimens of interior connection. The design parameter is the ratio of beam width to column width. Test results showed that cracks were distributed well and plastic hinges spread toward midspan without any significant degradation of strength and ductility. And the specimens sufficiently resisted up to the limiting drift ratio of 0.035 given by ACI of acceptance criteria for concrete special moment frames.