흰쥐 십이지장 점막의 Polyamine 대사, [^(14)C]Spermine 단백 결합능 및 [³H]Thymidine DNA 섭취에 대한 Calcitriol과 Prednisolone의 상호작용

최상현,이영재,정휘정,김형건,신경호,천연숙,전보권 고려대학교 의과대학 1997 고려대 의대 잡지 Vol.34 No.1

Calcitriol has been shown to induce the duodenal synthesis of the spermine-binding protein. and hydrocortisone was reported to increase the intestinal polyamine contents. This study examined whether prednisolone might enhance or reduce the calcitriol effects on the polyamine contents, [^(14)C]spermine-protein binding activity, and [^(3)H] thymidine DNA synthesis of the rat duodenum. The mucosal contents of putrescine. spermidine. and spermine were increased by 198.8%, 84.0%, and 78.3%, respectively, within 6 hours after calcitriol. 5 ㎍/kg (CT) and fell down to the control values at 12 hours. And prednisolone. 10 mg/kg (PD) also increased the polyamine contents in similar patterns. They did not counteract each other in the polyamine metabolism. CT increased the [^(14)C]spermine-binding activity of the cytosol protein fraction from the duodenal mucosa by 71.1%. But PD increased the binding activity by 28.0% and did not affect the CT-induced increase. CT increased the [^(3)H]thymidine DNA synthesis of the duodenal mucosa by 42.3%. but PD reduced it slightly. The CT-dependent increase of the synthesis was significantly inhibited by PD. These results suggested that the inhibitory effect of glucocorticoids on the vitamin D dependent increases of the duodenal calcium absorption and mucosal proliferation seems not to be associated with the metabolic changes of polyamines in the duodenal mucosa.

Arch교의 橫桁連結部 局部應力과 疲勞擧動에 관한 硏究

최광순,조호근,손영호,최광규 東亞大學校 建設技術硏究所 1997 硏究論文集 Vol.21 No.2

In this paper, the relation between the local stress and the fatigue behavior at gusset plate connected with main girder and floor beam in arch bridges was obtained. The conclusion was as follows : (1) In-plane bending moment and out-of-plane bending moment are higher than other internal stress resultants. (2) When a vehicle crosses the bridges, the change of out-of-plane bending moment corresponds with the change of relative difference in displacement in the longitudinal direction between the tie-girder and stringer. (3) The change of in-plane bending moment corresponds with the change of the displacement in the transverse direction of arch-rib. (4) Fatigue crack can be initiated by in-plane bending stress that is produced by out-of-plane bending moment at the toe of gusset plate. (5) Fatigue crack can be initiated by tension stress that is produced by in-plane bending moment at the toe of gusset plate.

경쟁적 효소면역검사를 이용한 혈청내 매독균 항체 검사의 유용성

최하영,박건,장숙진,박영진,문대수 조선대학교 부설 의학연구소 2000 The Medical Journal of Chosun University Vol.25 No.1

Background : Laboratory methods for detection of syphilis are Venereal Disease Research Laboratory (VDRL) and rapid plasma reagin (RPR) test to non-treponemal tests, and fluorescent treponemal antibody absorption (FTA-ABS) and microhemagglutination assay for antibodies to Treponema pallidum (MHA-TP) to treponemal tests. However, RPR card test had many false positive results and FTA-ABS IgM method required high degrees of technical ability and experience. Recently, enzyme immunoassay (EIA) kits for IgM and IgG antibodies to T. pallidum in syphilis have been developed. So, we evaluated that usefulness of competitive EIA for detection of T. pallidum antibody in serum. Methods : RPR positive 38 specimens and negative 6 specimens were compared by T. pallidum hemagglutination (TPHA), two commercial competitive EIA kits for detection of syphilis and FTA-ABS IgM. Those data were compared with concordant and discordant rate of each RPR, TPHA, EIA and FTA-ABS IgM. Results : 28 of 34 RPR positive specimens were TPHA(+) and EIA (+), and 6 of 6 RPR negative specimens were TPHA (-) and EIA (-). The data of two EIAs were same results with TPHA. 3 of 28 EIA positive specimens were FTA-ABS IgM (+), and 16 of 16 EIA negative specimens were FTA-ABS IgM (-). Conclusion: Two commercial competitive EIA kits for detection of syphilis were evaluated. The EIA method could be replaced with RPR and TPHA, and this method was possible to combination for detection of syphilis.

Arch교의 橫桁連結部 局部應力과 疲勞擧動에 관한 硏究

최광순,조호근,손영호,최광규 동아대학교 건설기술연구소 1997 硏究報告 Vol.21 No.2

In this paper, the relation between the local stress and the fatigue behavior at gusset plate connected with main girder and floor beam in arch bridges was obtained. The conclusion was as follows : (1) In-plane bending moment and out-of-plane bending moment are higher than other internal stress resultants. (2) When a vehicle crosses the bridges, the change of out-of-plane bending moment corresponds with the change of relative difference in displacement in the longitudinal direction between the tie-girder and stringer. (3) The change of in-plane bending moment corresponds with the change of the displacement in the transverse direction of arch-rib. (4) Fatigue crack can be initiated by in-plane bending stress that Is produced by out-of-plane bending moment at the toe of gusset plate. (5) Fatigue crack can be initiated by tension stress that is produced by in-plane bending moment at the toe of gusset plate.

오렌지 껍질로부터 분리된 프라보노이드의 화학요법 감작효과

최철익,변익건,차윤정,김선필,민영돈,유진철,송재경 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.2

Background and Objectives : The overexpression of P-glycoprotein (Pgp) or multidrug resistanceassociated protein (MRP) confers multidrug resistance (MDR) to cancer cells. MDR cells could be sensitized to anticancer drugs when treated concomitantly with a chemosensitizer. In this study, orange peel have been screened for the development of chemosensitizers reversing MDR. Materials and methods : Expression of Pgp in AML-2/WT and AML-2/D100 were determined using western blot analysis and RT-PCR. Candidates for chemosensitizers were isolated, purified from orange peel using sephadex LH-20 column chromatography and silica-gel column chromatography, whose structure was determined using ^1H-NMR, ^13C-NMR, ^1H-^1H-COSY, 13C-NMR, EI-Mass, IR. Chemosensitizing effect of NP-28 on AML-2/DX100 over expressing Pgp and MRP was determined by the MTT assay. Membrane toxicity was evaluated by the RBC hemolysis test. Antioxidant effects were examined by using a fluorescence probe (2’-7’-dichlorofluorescein diacetate). Results : A potent candidate, NP-28, has a flavonoid nucleus with some methoxy moiety. NP-28 is believed to have high therapeutic index, being non-transportable inhibitor and possibly, of inhibiting other efflux pumps such as MRP and antioxidant effects. NP-28 is also unlikely to be a substrate of Pgp. NP-28 did not increase Pgp levels. Conclusion : NP-28 has a Chemosensitizing effect to reverse Pgp-mediated MDR by increasing the intracellular accumulation of drugs. Furthermore, they do not increase expression of Pgp, which can allow a long term use without the concern about Pgp activation. NP-28 is anticipated as an ideal second-generation flavonoid chemosensitizer and/or possibly other therapeutic agents by further investigation.

주개 이온을 첨가한 강유전체 Bi₄Ti₃O_12(BIT) 박막의 특성

최은경,박문흠,김두회,구영건,김상수 昌原大學校 基礎科學硏究所 2001 基礎科學硏究所論文集 Vol.13 No.-

Bismuth layered structure ferroelectric thin films, BIT and donor (V, Nb and W) doped BIT, were prepared on the Pt/Ti/SiO2/Si substrates by sol-gel method. BIT film was characterized by strong c-axis prepared orientation. In the case of donor doped BIT thin films, the degree of c-axis prepared orientation decreased systematically V-BIT→W-BIT→Nb-BIT and Nb-BIT film was shown random orientation. Surface morphologies of BIT and V-BIT films had a plate-like and Nb-BIT and W-BIT had a mixture of plate-like and rod-like. In the results of ferroelectric hysteresis loops, the remanent polarization (2Pr) of random-oriented Nb-BIT capacitor was 26 μC/cm2, and this value was over two times higher than that of 12 μC/cm2 for BIT capacitor. The polarization fatigue up to 4.5×1010 read/write switching cycles of W-BIT capacitor was shown superior to the other capacitors. The polarization of the W-BIT capacitor decreased only 10% of the initial value after 4.5×1010 switching cycles.

제주 차귀도 연안해역 미생물 분포 및 이화학적 특성

문영건,김만철,이준백,송춘복,여인규,김종만,박근태,손홍주,최우봉,허문수 한국환경과학회 2006 한국환경과학회지 Vol.15 No.3

제대군인 취업지원 프로그램 구안

정철영,서우석,김성호,이예성,최동선,이종범,이성식,이건남,양안나,김성남 한국직업능력개발원 2007 직업능력개발연구 Vol.10 No.1

이 연구의 목적은 중·장기복무를 마치고 제대하여 재취업하고자 하는 제대군인들의 원활한 전직을 돕기 위한 제대군인 취업지원 프로그램의 개발방향과 개발안을 제시하는 데 있다. 이를 위해 국내·외에서 이루어지고 있는 제대군인 취업지원 프로그램의 실태를 분석하였고, 제대군인 취업지원 프로그램에 대한제대예정군인 및 제대군인과 기업체의 요구를 분석하여 시사점을 도출하였다. 그리고 이를 기초로 제대군인 취업지원 프로그램의 기본방향과 전체 흐름도, 세부 프로그램 개발안을 제시하였다. 이를 위해서 활용된 연구의 방법은 문헌연구, 조사연구, 전문가협의회 등이다. 이 연구를 통해서 제시된 제대군인 취업지원 프로그램의 개발방향과 개발안을 토대로, 일관되고 지속적인 수요자 중심의 제대군인 취업지원 프로그램의 개발이 가능할 것이다. The purpose of this study is to suggest plans and directions for developing. job-seeking support program for military veterans. The study is conducted through literature review, survey and interview. The results of this study are as follows: ⒧ basic development directions and flowchart of job-seeking support program for military veterans are suggested; and ⑵ development plans for sub-programs are suggested.

국내 호중구감소성 발열 환자에게 경험적 항진균제로 투여한 Amphotericin B deoxycholate와 Itraconazole 주사제의 비용-확인분석 : 예비보고

이동건,송영구,유진홍,최수미,최정현,박선희,신완식 대한감염학회 2004 감염과 화학요법 Vol.36 No.5

목적 : 진균감염이 점차 증가되고 있고 이를 치료하기 위한 비용 역시 증가하고 있지만 국내에서는 비용분석에 대한 연구가 거의 없는 실정이다. 본 연구에서는 호중구감소성 발열 환자에게 경험적 항진균제로 amphotericin B deoxycholate (ABV)혹은 정맥내 itraconazole (ITZA)을 투여할 때의 약물경제학적 측면을 후향적으로 비교해보고자 하였다. 재료 및 방법 : 같은 반응률을 보인다고 가정한 비용-확인분석 모델을 이용하여 가톨릭대학교 조혈모세포이식센터에서 혈액종양으로 항암치료 혹은 조혈모세포이식을 시행한 환자를 대상으로 자료를 수집하였다. 항진균제 투여에 대한 정보는 기존에 출판된 논문 및 학회 심포지움자료를 참고하였고, 논문에서 얻지 못한 정보는 가톨릭대학교 성모병원 의무기록실, 정보지원팀, 진료비관리팀의 협조를 받아 2003년 6월 1일부터 11월 30일까지의 자료를 분석하였다. ITZA에 대한 자료는 2003년 11월 제한적으로 1차 약제로 사용할 수 있게 되었던 때의 자료와 경험을 참조하였다. 결과 : 320명의 환자 중 149명(46.5%)에서 경험적 항진균제를 사용하였고 평균 투여일수는 ABV 17.0일, ITZA 9.8일이었다. 이상반응 발현율은 ABV 54%, ITZA 5%였다. ABV 투여환자 중 11명 (7.4%)에서 혈액투석을 평균 6.5일간 시행하였다. ITZA 투여시 간독성으로 혈장분리반출술, 인공간 등이 필요한 경우는 없었다. 경험적 항진균제를 ABV로 시작한 군(ABV군)은 1인당 1,229,495원, ITZA로 시작한 군(ITZA군)은 1인당 1,434,605원이 소요되어 ITZA군에서 205,110원 (16.7%)이 더 소요되었다. 평균 16일간 항진균제를 사용하여 1일 12,819.4원의 차이가 있었다. 결론 : 비용-확인 분석법에서 ITZA군이 ABV군 비해 하루 12,819.4원의 비용이 더 소요되었다. 비록 ITZA군에서 비용이 더 들었지만 1일 약가차이인 4.7배와 비교하여 많이 감소하여 항진균제의 비용을 1일 약가로 비교하는 것보다는 재원일수, 이상반응 발현율 등을 고려하는 총입원비용으로 약물경제학을 비교하는 것이 더 합당할 것으로 사료된다. 그 외에 환자의 예후, 삶의 질, 사회적 간접비용 등을 고려하는 전체적인 약물경제학을 비교하는 잘 계획된 전향적 연구가 추가로 필요하겠다. Background : The increasing incidence of systemic fungal infections and the rising medical cost have focused the need to determine the economic issues of antifungal agents. Nevertheless there have been only few reports about the cost analysis in Korea. We retrospectively investigated to compare the pharmacoeconomic aspects of amphotericin B deoxycholate (ABV) with those of intravenous itraconazole (ITZA) in the empirical treatment of febrile neutropenic patients. Materials and Methods : Through the cost-identification analysis model, on the presumption that two groups would show identical response, we collected data of patients who received hematopoietic stem cell transplantation (HSCT) or chemotherapy in Catholic HSCT center from Jun 1, 2003 to Nov 30, 2003. The literatures about information on antifungal therapy were extensively reviewed. Other unpublished information was provided by information assistant team, cost management team, and medical record unit in the same hospital. Results : Empirical antifungals were given to 149 out of 320 patients (46.5%). The average duration of administration were 17.0 days for ABV and 9.8 days for ITZA. The rates of adverse events were 54% and 5% for ABV and ITZA, respectively. Eleven (7.4%) patients given ABV received hemodialysis for 6.5 days in average. Among patients given ITZA, none developed hepatotoxicity requiring plasmapheresis or artificial liver. It cost 1,229,495 won/patient for the ABV group (starting ABV as empirical antifungal agent) and 1,434,605 won/patient for the ITZA group (starting ITZA as empirical antifungal agent) which was 16.7% more expensive than ABV group. When we compare the average daily cost for 16 days, the ITZA group spent 12,819.4 won/patient/day more than the ABV group. Conclusion : The cost-identification model in this study revealed that ITZA group cost more than ABV group. However, the difference in expense decreased when 1 day-cost was compared (4.7 times greater than ABV). It would be more reasonable to consider the total cost of anti-fungals during the hospitalization rather than 1 day cost in considering pharmacoeconomics. Well designed prospective study considering prognosis of patients, quality of life, and indirect social cost should be warranted.

경상북도 전문계 고등학교 발전방안

정철영,최홍영,이광호,김재호,이건남,최수정 한국농업교육학회 2008 농업교육과 인적자원개발 Vol.40 No.3

이 연구의 목적은 경상북도에 소재하고 있는 전문계 고등학교가 발전할 수 있도록 하는 방안을 제시하는 데 있다. 이 연구는 다음과 같은 과정을 통해서 수행되었다. 첫째, 전문계 고등학교 관련 정책, 전문계 고등학교의 교육여건, 지역 산업여건 및 전망, 전문계 고등학교 진단, 교육수요자들을 대상으로 요구조사를 하였다. 둘째, 경상북도 전문계 고등학교의 내적, 외적 사항을 고려하여 발전방향을 설정하였다. 셋째, 경상북도에 소재한 전문계 고등학교 발전을 위한 기본방향을 설정하고 그에 따른 추진과제를 제시하고, 단위학교별, 계열별, 권역별 발전 전략, 행·재정적 지원 방안을 제시하였다. 이 연구의 결과로는 첫째, 경상북도 전문계 고등학교 발전을 위한 5대 기본방향, 15대 추진과제, 45대 세부추진과제를 수립하였다. 둘째, 경상북도에 소재하고 있는 각 전문계 고등학교별로 전문화 및 특성화 영역, 발전 기본방향, 발전방안, 우선 해결과제, 지원 요청 사항을 포함한 단위학교 발전방안을 수립하였다. 셋째, 경상북도에 소재하고 있는 전문계 고등학교의 계열의 SWOT분석을 실시하고, 계열발전방향, 발전과제 및 전략을 포함한 계열별 발전방안을 수립하였다. 넷째, 경상북도를 4개의 권역으로 구분하고 각 권역별 인구추이, 학교수, 학생수, 중점사업 및 산업발전계획 내용을 기초로 하여 권역에 따른 계열별 발전방향 및 발전방안을 제시하였다. 다섯째, 전체 발전방안, 단위학교, 계열, 권역별 발전 방안이 잘 수행될 수 있도록 행·재정적인 지원 전략을 수립하였다. The purposes of this study were to suggest directions to promote vocational high schools in Gyeongsangbuk-do by adapting actively to internal and external environment changes, and corresponding to regional characteristics and industrial demands. The study was performed as follows: (1) analyzing the external and internal surroundings of vocational high schools in Gyeongsangbuk-do such as related educational policies, conditions and prospects of regional industry, community member's notices and needs, educational conditions and self development plan of vocational high schools, (2) establishing the strategic criteria selected for promoting the vocational high schools by the results of the first, (3) based on the strategic criteria, establishing the basic directions for promoting the vocational high schools in Gyeongsangbuk-do and development strategies by schools, fields, and regions. The following five findings are the results of this study. (1) S basic directions, 15 themes, and 45 sub-themes of the vocational high school development were established. (2) Promoting plans for all 72 vocational high schools were provided in terms of basic directions, development strategies, and specialization. (3) Promoting plans for vocational high school fields such as agricultural high school, technical high school, commercial high school, and home economics high school were presented. (4) Based on the industrial strategies in Gyeongsangbuk-do, promoting plans for four provinces were presented. (5) Administrational and financial strategies were suggested in order to support for promoting vocational high schools in Gyeongsangbuk-do.