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최우봉 동의대학교 산업기술개발연구소 2005 産業技術硏究誌 Vol.19 No.-
The early applications of microarrays and detection technologies have been centered on DNA-based applications. The application of array technologies to proteomics is now occurring at a rapid rate. Numerous researchers have begun to develop technologies for the creation of microarrays of protein-based screening tools. The stability of antibody molecules when bound to surfaces has made antibody arrays a starting point for proteomic microarray technology. To minimize disadvantages due to size and availability, some researchers have instead opted for antibody fragments, antibody mimics or phage display technology to create libraries for protein chips. Even further removed from antibodies are libraries of aptamers, which are single-stranded oligonucleotides that express high affinity for protein molecules. A variation on the theme of protein chips arrayed with antibody mimics or other protein capture ligand is that of affinity MS where the protein chips are directly placed in a mass spectrometer for detection. Other approaches include the creation of intact protein microarrays directly on glass slides or chips. Although many of the proteins may likely be denatured, successful screening has been demonstrated. The investigation of protein-protein interactions has formed the basis of a technique called yeast two-hybrid. In this method, yeast "bait" proteins can be probed with other yeast "prey" proteins fused to DNA binding domains. Although the current interpretation of protein arrays emphasizes microarray grids of proteins or ligands on glass slides or chips, 2-D gels are technically macroarrays of authentic proteins. In an innovative departure from the traditional concept of proteinchips, some researchers are implementing microfluidic printing of arrayed chemistries on individual protein spots blotted onto membranes. Other researchers are using in-jet printing technology to create protein microarrays on chips. The rapid growth of proteomics and the active climate for new technology is driving a new generation of companies and academic efforts that are developing novel protein microarray techniques for the future.
최우봉 한국식물병리학회 2003 Plant Pathology Journal Vol.19 No.1
The ascomycete Magnaporthe grisea is a pathogen of rice blast and is known to form specialized infection structures called appressoria for successful infection into host cells. To understand the molecular mechanism underlying infection process, appressorium-related genes were identified through global approaches including EST sequencing, differential hybridization, and suppression subtractive hybridization. EST database was generated on >2,000 cDNA clones randomly selected from appressorium stage cDNA library. Large number of ESTs showed homology to known proteins possibly involved in infection-related cellular development (attachment, germination, appressorium formation, and colonization) of rice blast fungus. The 1051 ESTs showing significant homology to known genes were assigned to 11 functional categories. Differential hybridization and suppression subtractive hybridization were applied to identify genes showing an appressorium stage specific expression pattern. A number of genes were selected as up-regulated during appressorium formation compared with the vegetative growing stage. Clones from various cDNA libraries constructed in different developmental stages were arrayed on slide glass for further expression profiling study. Functional characterization of genes identified from these global approaches may lead to a better understanding of the infection process of this devastating plant disease, and the development of novel ways to protect host plant.
서생군,최우봉,홍세진,김병섭 한국식물병리학회 2014 Plant Pathology Journal Vol.30 No.1
The bacterial strain T-9, which shows strong antifungalactivity, is isolated from the soils of Samcheok, Gangwondoand identified as Paenibacillus kribbensis accordingto morphological and taxonomic characteristics and16S rRNA gene sequence analysis. The P. kribbensisstrain T-9 strongly inhibits the growth of variousphytopathogenic fungi including Botrytis cinerea,Colletotricum acutatum, Fusarium oxysporum f. sp. radicis-lycopersici, Magnaporthe oryzae, Phytophthoracapsici, Rhizoctonia solani, and Sclerotium cepivorumin vitro. Also, the P. kribbensis strain T-9 exhibitedsimilar or better control effects to plant diseases thanin fungicide treatment through in vivo assays. In the2-year greenhouse experiments, P. kribbensis strainT-9 was highly effective against clubroot. In the 2-yearfield trials, the P. kribbensis strain T-9 was less effectivethan the fungicide, but reduced clubroot on Chinesecabbage when compared to the control. The abovedescribedresults indicate that the strain T-9 mayhave the potential as an antagonist to control variousphytopathogenic fungi.
Rhizoctonia solani에 의한 결구상추 밑둥썩음병 방제균주 Stenotrophomonas maltophilia BW-13의 분리 및 동정
김한우,최우봉,이진우,문병주,이광렬,박종영,김현주 한국식물병리학회 2005 식물병연구 Vol.11 No.2
In a course of searching for biofungicide to control crisphead lettuce bottom rot caused by Rhizoctonia solani, we have isolated an antagonistic bacterium from lettuce rhisophere soil. A total of 702 bacterial isolates were isolated and tested for in vitro growth inhibition of R. solani. Seven strains appeared to have strong antagonistic effect against R. solani in in vitro growth inhibition assay. In the pot experiments, a strain BW-13 showed the most potent disease control effect on the both lettuce seedlings and adults plants. Therefore, the BW-13 was selected as a biocotrol candidate against crisphead lettuce bottom rot. Based on its morphology, physiological characteristics, and 16S rRNA gene analysis, the BW-13 was finally identified as Stenotrophomonas maltophilia. This study indicated that S. maltophilia BW-13 could be used as a biocontrol agent to control crisphead lettuce bottom rot. 결구상추 밑둥썩음병의 병원균 Rhizoctonia solani에 대한 길항세균을 분리하기 위하여 결구상추 재배 포장에서 결구상추의 밑둥부위, 뿌리조직 그리고 근권토양에서 세균 702균주를 분리하였다. 분리균주 중에서 대치배양에 의해 길능을 나타낸 7균주를 선발하였으며, 그 중 포 트검정 실험에서 유묘와 성체식물에서 발병억제효과가 우수한 BW-13균주를 길항균으로 최종선발하였다. 최종 선발된 BW-13균주의 동정을 위하여 생리·생화학적 특성, API test 및 16S rDNA sequence를 분석한 결과 notrophomonas maltophilia로 동정되었으며, 본 균주를 S. maltophilia BW-13으로 명명하였다. 본 연구의 결과는 S. maltophilia BW-13이 결구상추 밑둥썩음병의 생물적 방제원으로 이용될 수 있음을 보여준다.
Phytophthora Species, New Threats to the Plant Health in Korea
현익화,최우봉 한국식물병리학회 2014 Plant Pathology Journal Vol.30 No.4
Given the lack of a resistant genetic pool in host plants,the introduction of exotic invasive pathogens can resultin epidemics that affect a specific ecosystem andeconomy. Plant quarantine, which is designed to protectendemic plant resources, is a highly invaluablesafeguard that should keep biosecurity with increasinginternational trade and global transportation. A totalof 34 species of plant pathogens including Phytophthorainfestans were documented as introduced from othercountries into Korea from 1900 to 2010. The genusPhytophthora, classified in oomycetes, includes morethan 120 species that are mostly recognized worldwideas highly invasive plant pathogens. After 2000, over 50new species of Phytophthora were identified internationallyas plant pathogens occurring in crops and foresttrees. In Korea, Phytophthora is also one of the mostserious plant pathogens. To date, 22 species (about onefifthof known species) of the genus have been identifiedand reported as plant pathogens in the country. Thelikelihood of new exotic Phytophthora species being introducedinto Korea continues to increase, thus necessitatingintensive plant quarantine inspections. As newpotential threats to plant health in Korea, six Phytophthoraspecies, namely, P. alni, P. inundata, P. kernoviae,P. pinifolia, P. quercina, and P. ramorum, are discussedin this review with focus on history, disease, biology,management, and plant quarantine issues.Invasive exotic plant pathogens and Phytophthoraspp.