KOVA™ System을 이용한 요침사 검사에 대한 검토

문대수,장숙진 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.15 No.1

Analysis of urine specimen for various clinically significant constituent have long been a well establshed laboratory procedure. Microscopic examination of urinary sediment is very useful test for detection genito-uhnary tract disease. The methods for assessing the excretion of elements in the urine are certainly unreliable. In the most requently used method excretion is assessed per high power field(HPF). This is not quantitative and lot a precise account of the method used. Nevertheless this method continue to be used without challnge. A commercial system is available for the standardization of urine microscopic determinations. The KOVA^(TM) system (ICL Scientific, Fountain Valley, Calif.) offers a graduated tube to measure exact olumes of urine for centhfugation, a transfer pipette to decant specimens and retain exactly 1ml of rine resuspension of the sediment and slide chamber for microscopic examination. The results are as follows ; 1. The reprodudbility of results (coefficient of variation : C.V.) from conventional method and KOVA^(TM) system are 44%, 19% in RBC and 43%, 15% in WBC sediment. 2. The number of WBC/HPF in KOVA^(TM) system(x) is good correlation with the WBC/㎟ in chamber count(y) (regression equation : y=10.078x-7.780, correlation coefficient(r)=0.989) 3. There was no relationship between the sediment abnormalities by KOVA^(TM) system and specific gravity of urine.

광주지역 소아 환아의 무균성 뇌막염 원인바이러스 규명

문대수,신명근 대한감염학회 2001 감염 Vol.33 No.4

Background : Aseptic meningitis is a common illness of children. It seems that viruses are the usual etiologic agents. The distribution of these agents mainly depends on the isolated time and region area. This study was performed to isolate the causative viruses from patients with aseptic meningitis in Gwanju area during recent one year. Methods : A total of 130 patients with aseptic meningitis were evaluated, Stool and/or cerebrospinal fluid specimens from patients were inoculated into rhabdomyosarcoma (RD), HEp2 and Vero cell lines. The virus propagation was examined by the presence of cytopathic effects. Neutralizing tests using enterovirus serum pool were done on each viral isolates. Results : The isolation rate of enterovirus was 24.6% (32/130). The enterovirus isolates were obtained mostly from stool specimens (29/32). Twenty-two isolates were identified by neutralizing test. Ten isolates disclosed 'untyping' by neutralizing test. The distribution of isolates was coxsackievirus group B2 (11 stains, 34.4%), echovirus 30 (4 strains, 12.5%), echovirus 6 (3 strains, 9.4%), echovirus 9, 11, 25 and coxsackievirus group Al6 (1 strain, respectively). These strains were predominantly isolated during summer season (June to July). Conclusions : The causative viruses from patients with aseptic meningitis in Gwanju area during recent 1 year were coxsackievirus group B2, echovirus 30, 6, 9, 11, 25 and coxsackievirus group A16 which were mostly isolated from stool specimens in summer season. (Korean J Infect Dis 33:248∼253, 2001)

세균뇨의 선별 검사법에 관한 연구

문대수,손장신,박영진,정용호 대한임상병리학회 1986 대한임상병리학회지 Vol.6 No.1

Micral-Test^TM을 이용한 미세단백뇨 측정의 임상적 유용성

문대수,이국일 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.1

The excretion of small quantities of urinary albumin (microalbuminuria) might the earliest clinical sign of incipient diabetic nephropathy and important predictor of renal failure in diabetes. Currently methods available to detect microalbuminuria were not suitable for routine screening. A test strip (Micral-Test_TM : Boehringer Mannheim GmbH, Germany) based on dry immnunochemical principles had recently been developed which allows semiquantitative detection of urinary low albumin concentration. We measured microalbuminuria by Micral-Test_TM and compared with any other items. The results were summerized as follows : 1. The relationships of the duration of diabetes to microalbuminuria was low positive association (r=0.332l). 2. Results obtained by Micral-Test_TM correlated closely with values obtained by rate nephelometry (r=0.7998). 3. There were statistically significant difference in BUN between positive and negative results of Micral-Test_TM(p<0.05), but no significant difference in FBS TG, total cholesterol, creatinine and BP . 4. There were statistically significant difference in BUN, creatinine BP between positive and negative results of N-Multistix SG_TM(p<0.001, p<0.01, p<0.05), but no significant difference in FBS, TG, total cholesterol. 5. The detection discrepancy rate of albuminuria in diabetes between Micral-Test_TM and N-Multis- tix SG_TM was 12.9%. In conclusioin, Micral-Test_TM was a good screening method for easy and quick detection of microalbuminuria and monitoring the development of urinary albumin excretion in the microalbuminuric range.

Reagent Strip(Ames N-Multistix(r) SG)을 이용한 요 비중 검사성적의 비교검토

문대수,정용호,박영진 대한임상병리학회 1984 대한임상병리학회지 Vol.4 No.2

전기영동상에 나타난 Extra Lactate Dehydrogenase Isoenzyme Band

문대수,황호원,송혜숙,박영진,정용호 대한임상검사정도관리학회 1984 臨床檢査와 精度管理 Vol.6 No.1

호흡기 감염 환자에서 분리된 Mycobacterium abscessus 1예

오혜룡 · 장숙진 · 이학민 · 이명희 · 박 건 · 문대수 · 박영진 조선대학교 의학연구원 2008 The Medical Journal of Chosun University Vol.33 No.3

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혈청내 B형 간염 바이러스 DNA 정량을 위한 실시간 중합효소연쇄반응분서기 성능비교

장숙진 · 한호림 · 박 건 · 김영숙 · 기승정 · 신성희 · 정옥연 · 문대수 · 박영진 조선대학교 의학연구원 2008 The Medical Journal of Chosun University Vol.33 No.1

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응급환자에서 발견한 Cis-AB 가족1예

박영진,문대수,장숙진,이호영,황호원 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.15 No.1

Cis-AB is a rare blood group showing unusual inheritance of chromosomal crossing over and mutation in the ABO group, Clinically we may contact how this patient with a rare blood group should be controlled in the emergency. Authors experienced a case of Cis-AB in the emergency event and reported that with brief review literature.

Vibrio 임상 분리주의 균종 동정을 위한 dnaJ 및 16S rDNA의 서열 분석법 비교

최인선,문대수,박건,강성호,김춘미,안영준,김동민,윤나라,임동훈,신성희,국중기,장영효,장숙진 대한진단검사의학회 2018 Laboratory Medicine Online Vol.8 No.1

배경: Vibrio 종에는 치명적인 패혈증을 일으키는 균종들도 포함되어 있어서 정확한 균종 동정이 매우 중요하다. 일부 비전형적인표현형을 보이는 균종이 있어 정확한 동정을 위해 적절한 분자 진단법의 도입이 필요하다. 방법: 본 연구에서는 Vibrio 임상 분리주 53주와 표준균주 8주가이용되었다. 분자 진단법으로는 dnaJ 유전자 서열 분석, 16S rDNA 서열 분석, gyrB V. vulnificus–특이적 PCR 서열 분석, gyrB V. navarrensis– 특이적 PCR 서열 분석, V. vulnificus hemolysin 유전자(vvh) PCR (V. vulnificus-특이적 PCR)를 시행하였다. 또한 16S rDNA 와 dnaJ 유전자, gyrB 유전자의 서열분석 결과를 토대로 계통수분석을 실시하였으며 상기 검사 결과를 종합하여 균의 최종 동정명이 결정되었다. 16S rDNA 서열 분석과 dnaJ 유전자 서열 분석 간의 일치율 분석은 카이 제곱 검정을 이용하였다. 결과: 61주의 Vibrio 균주의 최종 균종명 분포를 내림차순으로 배열하면 다음과 같다: V. vulnificus (78.69%), V. parahaemolyticus (6.56%), V. navarrensis (4.92%), V. mimicus (1.64%), V. cholera (1.64%), V. furnissii (1.64%), V. alginolyticus (1.64%), Grimontia hollisae (1.64%). dnaJ 유전자 서열 분석의 정확도는 91.80%였고16S rDNA 서열 분석의 정확도는 86.89%였다. dnaJ 유전자 서열 분석법과 16S rDNA 서열 분석법 간의 일치도는 0.45로서, 중등도의일치도였다. 결론: 본 연구는 dnaJ 유전자 서열 분석법이 서로 밀접히 관련된Vibrio 종 간의 정확한 균 종정에 유용한 방법이라는 것을 보여주었다. Background: Among the many Vibrio species that can cause infections in humans, several species can cause a fatal outcome. Therefore, accurate identification of Vibrio species is very important. Since some species show atypical phenotypic features, selecting an appropriate molecular method is necessary to avoid misdiagnosis. Methods: Vibrio clinical isolates (N=53) and reference strains (N=8) were used in this study. We analyzed the following sequences for identification: dnaJ gene, 16S rDNA, gyrase B (gyrB) V. vulnificus–specific sequence, gyrB V. navarrensis–specific sequence, and V. vulnificus hemolysin gene PCR (Vvh PCR). We performed phylogenetic analysis of the 16S rDNA, dnaJ, and gyrB sequences. Final identification was based on the combined results of all tests described above. Concordance of the 16S rDNA and dnaJ sequence analysis was measured using the Chi-square test. Results: The 61 Vibrio strains were identified as follows, in descending order: V. vulnificus (78.69%), V. parahaemolyticus (6.56%), V. navarrensis (4.92%), V. mimicus (1.64%), V. cholera (1.64%), V. furnissii (1.64%), V. alginolyticus (1.64%), and Grimontia hollisae (1.64%). The accuracy rates of the dnaJ gene and 16S rDNA sequence for identification were 91.80% and 86.89%, respectively. The 16S rDNA and dnaJ sequences showed a concordance rate of 0.45, which indicates moderate agreement. Conclusions: Our results suggest that analysis of the dnaJ sequence may be a useful method for the identification of clinical isolates of Vibrio species, especially for distinguishing between closely related Vibrio species.