Schisandrin B Improves the Hypothermic Preservation of Celsior Solution in Human Umbilical Cord Mesenchymal Stem Cells

Zhang Ying,Wang Peng,Jin Mei-xian,Zhou Ying-qi,Ye Liang,Zhu Xiao-juan,Li Hui-fang,Zhou Ming,Li Yang,Li Shao,Liang Kang-yan,Wang Yi,Gao Yi,Pan Ming-xin,Zhou Shu-qin,Peng Qing 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.3

BACKGROUND: Human umbilical cord mesenchymal stem cells (hUCMSCs) have emerged as promising therapy for immune and inflammatory diseases. However, how to maintain the activity and unique properties during cold storage and transportation is one of the key factors affecting the therapeutic efficiency of hUCMSCs. Schisandrin B (SchB) has many functions in cell protection as a natural medicine. In this study, we investigated the protective effects of SchB on the hypothermic preservation of hUCMSCs. METHODS: hUCMSCs were isolated from Wharton’s jelly. Subsequently, hUCMSCs were exposed to cold storage (4 C) and 24-h re-warming. After that, cells viability, surface markers, immunomodulatory effects, reactive oxygen species (ROS), mitochondrial integrity, apoptosis-related and antioxidant proteins expression level were evaluated. RESULTS: SchB significantly alleviated the cells injury and maintained unique properties such as differentiation potential, level of surface markers and immunomodulatory effects of hUCMSCs. The protective effects of SchB on hUCMSCs after hypothermic storage seemed associated with its inhibition of apoptosis and the anti-oxidative stress effect mediated by nuclear factor erythroid 2–related factor 2 signaling. CONCLUSION: These results demonstrate SchB could be used as an agent for hypothermic preservation of hUCMSCs.

Testing Investigation of Protective Coatings for Downhole Oil Tube

( Li Ping Zhang ),( Qi Bin Zhang ),( Yan Jun Zhang ),( Bei Bei Xie ),( Ying Ying Zhang ) 한국부식방식학회(구 한국부식학회) 2008 Corrosion Science and Technology Vol.7 No.1

The solvent-free modification of butyl rubber in the medium of NaH

Ying Feng,Yan Xia Wang,Hong Qi Shao,En Hua Zhang,Zhen Wang,Jiruo Zhao 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.1

A novel improved butyl rubber (PMIIR) was prepared in a solvent free process, using sodium hydride(NaH) as activating agent and maleic anhydride (MAH) as modifier by processing modificationtechnology in a conventional polymer processing equipment of an internal mixer. The structure ofproducts was characterized by 1H NMR and FT-IR. In this process, polar sodium carboxylate functionalgroup and unsaturated carbon–carbon double bonds were successfully introduced onto the chains of IIRon one step. The reaction mechanism was investigated in detail. It was found that the functionalizationexperienced two modified mechanisms: addition and substitution modified mechanisms. The reactionefficiency and content of MAH were evaluated. Polymer isolation and purification operations werereduced out due to the result of solvent free. Curing and mechanical properties of products wereinvestigated. It was found that PMIIR has greatly increased curing rate due to the incorporation of polargroup and carbon–carbon double bond.

Overexpression of microRNA-612 Restrains the Growth, Invasion, and Tumorigenesis of Melanoma Cells by Targeting Espin

Ying Zhu,Hao-liang Zhang,Qi-ying Wang,Min-jing Chen,Lin-bo Liu 한국분자세포생물학회 2018 Molecules and cells Vol.41 No.2

microRNA (miR)-612 shows anticancer activity in several types of cancers, yet its function in melanoma is still unclear. This study was undertaken to investigate the expression of miR-612 and its biological relevance in melanoma cell growth, invasion, and tumorigenesis. The expression and prognostic significance of miR-612 in melanoma were examined. The effects of miR-612 overexpression on cell proliferation, colony formation, tumorigenesis, and invasion were determined. Rescue experiments were conducted to identify the functional target gene(s) of miR-612. miR-612 was significantly downregulated in melanoma tissues compared to adjacent normal tissues. Low miR-612 expression was significantly associated with melanoma thickness, lymph node metastasis, and shorter overall, and disease-free survival of patients. Overexpression of miR-612 significantly decreased cell proliferation, colony formation, and invasion of SK-MEL-28 and A375 melanoma cells. In vivo tumorigenic studies confirmed that miR-612 overexpression retarded the growth of A375 xenograft tumors, which was coupled with a decline in the percentage of Ki-67-positive proliferating cells. Mechanistically, miR-612 targeted Espin in melanoma cells. Overexpression of Espin counteracted the suppressive effects of miR-612 on melanoma cell proliferation, invasion, and tumorigenesis. A significant inverse correlation (r = -0.376, P = 0.018) was observed between miR-612 and Espin protein expression in melanoma tissues. In addition, overexpression of miR-612 and knockdown of Espin significantly increased the sensitivity of melanoma cells to doxorubicin. Collectively, miR-612 suppresses the aggressive phenotype of melanoma cells through downregulation of Espin. Delivery of miR-612 may represent a novel therapeutic strategy against melanoma.

Research on Friction Characteristics of AlCrN and TiAlSiN Coatings and Properties of Coated Tools

Qi-Bin Yue,Hui-Bo He,Hua-Ying Li,Jun Zhang,Yuan-ming Li,Lu Ma 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.20 No.9

AlCrN and TiAlSiN coatings were deposited on the surface of 30CrMnSi steel and cemented carbide YT15 by multi-arc ion plating technique. A scanning electron microscopy was used to observe the surface morphologies of both coatings. The reciprocating sliding tests of AlCrN and TiAlSiN coating were performed to investigate the friction coefficients and the wear mechanisms of both coatings were analyzed as well. Dry machining tests on 30CrMnSi hardened steel were carried out with the AlCrN and TiAlSiN coated tools on a CA6140A lathe. The effects of cutting speed on cutting forces and cutting temperatures of AlCrN and TiAlSiN coated tools were obtained and analyzed. The microcosmic micrographs of wear areas of both coated tools were observed and investigated by scanning electron microscope and energy dispersive spectrum. The results show that the hardness and bonding strength of AlCrN coating are higher than that of TiAlSiN coating, while the friction coefficient of AlCrN coating is lower than that of TiAlSiN coating. Both the cutting force and cutting temperature of AlCrN coated tool are lower than TiAlSiN coated tool. The time required for the bluntness of the AlCrN coated tool is approximately 33.3% longer than that of the TiAlSiN coated tool, and the main wear mechanisms of both tools are mainly crater wear, diffusion wear and oxidation wear.

Comparative Proteomic Analysis of Changes in the Bovine Whey Proteome during the Transition from Colostrum to Milk

Zhang, Le-Ying,Wang, Jia-Qi,Yang, Yong-Xin,Bu, Deng-Pan,Li, Shan-Shan,Zhou, Ling-Yun Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.2

Bovine whey protein expression patterns of colostrum are much different from that of milk. Moreover, bovine colostrum is an important source of protective, nutritional and developmental factors for the newborn. However, to our knowledge, no research has been performed to date using a comparative proteomic method on the changes in the bovine whey proteome during the transition from colostrum to milk. This study therefore separated whey protein of days 1, 3, 7 and 21 after calving using two dimension electrophoresis. Differentially expressed proteins at different collection times were identified using high-performance liquid chromatography in tandem with mass spectrometry (LC/MS) and validated by enzyme-linked immunosorbent assay (ELISA) in order to understand the developmental changes in the bovine whey proteome during the transition from colostrum to milk. The expression patterns of whey protein of days 1 and 3 post-partum were similar except that immunoglobulin G was down-regulated on day 3, and four proteins were found to be down-regulated on days 7 and 21 compared with day 1 after delivering, including immunoglobulin G, immunoglobulin M, albumin, and lactotransferrin, which are involved in immunity and molecule transport. The results of this study confirm the comparative proteomic method has the advantage over other methods such as ELISA and immunoassays in that it can simultaneously detect more differentially expressed proteins. In addition, the difference in composition of milk indicates a need for adjustment of the colostrum feeding regimen to ensure a protective immunological status for newborn calves.

Epidemiological Characteristics of Norovirus Outbreaks in Shenyang from 2017 to 2021

Qi Ying,Dong Xinxin,Cheng Xiaowei,Xu Han,Wang Jin,Wang Bing,Chen Ye,Sun Baijun,Zhang Linlin,Yao Yan 한국미생물학회 2023 The journal of microbiology Vol.61 No.4

Norovirus is one of the leading causes of acute gastroenteritis outbreaks worldwide. This study aimed to identify the epidemiological characteristics of norovirus outbreaks and to provide evidence for public health entities. Specimens and epidemiological survey data were collected to determine if there were differences in the attack rate of norovirus in terms of the year, season, transmission route, exposure setting, and region and to determine whether there were relationships between the reporting interval, the number of illnesses in a single outbreak and the duration of the outbreak. Norovirus outbreaks were reported throughout the year, with seasonal characteristics (i.e., high rates in spring and winter). Among all regions in Shenyang with the exception of Huanggu and Liaozhong, norovirus outbreaks had been reported, and the primary genotype was GII.2[P16]. Vomiting was the most common symptom. The main places of occurrence were childcare institutions and schools. The person-to-person route was the main transmission route. The median duration of norovirus was 3 days (IQR [interquartile range]: 2–6 days), the median reporting interval was 2 days (IQR: 1–4 days), the median number of illnesses in a single outbreak was 16 (IQR: 10–25); there was a positive correlation between these parameters. Norovirus surveillance and genotyping studies still need to be further strengthened to increase knowledge regarding the pathogens and their variant characteristics, to better characterize the patterns of norovirus outbreaks and to provide information for outbreak prevention. Norovirus outbreaks should be detected, reported and handled early. Public health entities and the government should develop corresponding measures for different seasons, transmission routes, exposure settings, and regions.

Novel efficient and environmentally friendly recovering of high performance nano-LiMnPO4/C cathode powders from spent LiMn2O4 batteries

Qi Meng,Jianguo Duan,Ying-Jie Zhang,Peng Dong 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.80 No.-

In this study, a novel efficient and environmentally friendly recycling process is developed to recoverspent LiMn2O4 powder as raw materials for high-performance LiMnPO4/C nanocomposites. The processcomprises a mechanochemical liquid-phase activation of the precursor mixture followed by a single-stepsolid-state heat treatment. The results indicate that the recovered LiMnPO4/C nano-composite has apromising electrochemical performance with discharge capacities of 148.5, 136.1, and 116.5 mA h g 1 at0.05, 0.2, and 1C rate, respectively, in the voltage range of 2.5–4.5 V. A perfect cyclic performance is alsodisplayed with a capacity retention of 98% after 100 cycles at 1C rate.

Use of grape seed as reductant for leaching of cobalt from spent lithium-ion batteries

Ying-Jie Zhang,Qi Meng,Peng Dong,Jianguo Duan,Yan Lin 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.66 No.-

The grape seed was novelly used as reductant for leaching of spent LiCoO2 material, which is idea of “waste + waste → resources”. About 92% Co and 99% Li could be leached under the optimized conditions of grape seed 0.6 g/g, malic acid 1.5 mol/L, 180 min, 80 °C, and slurry density 20 g/L. The catechin, EC and EGCG contained in grape seed could be employed as efficient reductants during leaching. The leaching process is controlled by combination of surface chemical reaction and diffusion with apparent activation energy of 11.96 kJ/mol, which is related to the form of Co(OH)3.

The evaluation of online course of Traditional Chinese Medicine for Medical Bachelor, Bachelor of Surgery international students during the COVID-19 epidemic period

Zhang Qing,He Yi-Jing,Zhu Yu-Hang,Dai Min-Chen,Pan Man-Man,Wu Jia-Qi,Zhang Xian,Gu Ying-Er,Wang Fang-Fang,Xu Xiang-Rong,Qu Fan 한국한의학연구원 2020 Integrative Medicine Research Vol.9 No.3

Background: During the COVID-19 epidemic period, Traditional Chinese Medicine (TCM) course for international students of Medical Bachelor, Bachelor of Surgery (MBBS) program in Zhejiang University has shifted from traditional classroom to online environment. This study aimed to investigate MBBS international students’ perception on online TCM course, and to assess the online learning efficacy. Methods: A total of 84 MBBS international students attending course of “Basic Traditional Chinese Medicine” during 2020 academic years at Zhejiang University were enrolled in this study. A quantitative questionnaire was respectively completed before and after the TCM course using a pretest–post-test design. By means of two online learning platforms, Learning in ZJU and DingTalk, TCM course was broadcast in both live and archived format to students. Results: A total of 48 participants completed both baseline and follow-up questionnaires. The majority of participants preferred face-to-face classroom learning (26, 54.17% of total) when compared with online learning. Students felt that the course had brought in much benefits (mean 3.88, SD 0.87), and they were satisfied with the course content (mean 3.83, SD 0.95). Students’ TCM related knowledge and their behaviors of discussion and consulting were significantly improved by online TCM course (all P < 0.001). Students’ awareness of the necessity of TCM education and their feeling of difficulty in learning TCM were significantly strengthened (P = 0.042, 0.025, respectively). Conclusion: Online learning is a good alternative for TCM course of MBBS international students when classroom learning is suspended, whereas it cannot replace the need for onsite and face-to-face learning.