Analysis of the Crack Evolution Process in Crumb Rubber Concrete Based on Acoustic Emission Technology

Pan Ming,Jun Lu,Xin Cai,Miaoyan Liu,Xudong Chen 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.7

In this study, the crack evolution process of crumb rubber concrete (CRC) in a four-point bending tensile test was monitored using the acoustic emission (AE) technique. The peak frequency, distribution characteristics of the AE source, and the damage process based on the AE ring count during the crack evolution were analyzed. The test results revealed that the addition of rubber particles decreased the brittleness of cracks and improved the ductility of the concrete during the macro-cracking process. The AE positioning method could effectively monitor the evolution process of micro-cracks inside the concrete. Additionally, based on the statistical results of the AE source events and energy distribution, the position of macroscopic cracks could be predicted, and the random distribution of micro-cracks could be characterized. By introducing the Weibull distribution to describe the random growth process of micro-cracks, the damage inside the concrete could be qualitatively predicted. As the rubber particle content increased, the inherent micro-cracks inside the concrete also increased. The damage inside the concrete was biased toward the crack nucleation stage.

A Key Frame Extraction Algorithm Based on Clustering and Compressive Sensing

Lei Pan,Xin Shu,Ming Zhang 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.11

Key frame extraction is considered as one of the most critical issues in content-based video retrieval technology (CBVR). In this paper, an efficient key frame extraction algorithm based on unsupervised clustering and compressive sensing is proposed. Firstly, three types of filters with various scales are employed to generate high dimensional feature of each frame in one shot, which will be projected to low dimensional feature by a very sparse random projection matrix that satisfies the condition of Restricted Isometry Property (RIP), and then sub-shot segmentation is conducted by an unsupervised clustering method in order to divide one shot into sub-shot collections, in which each class of clustering represents one sub-shot. Finally, the Bhattacharyya coefficient is used to measure the similarity between frame and class center, the frame with the maximum similarity value is selected as the key frame in each sub-shot. The experimental results demonstrate that the proposed method could extract key frames efficiently and properly.

Adsorption of Fluoride Ions from Water by SF/PP Nonwoven Fabrics

Zuoze Fan,Yanfei Gao,Xin Ning,Fukui Pan,Jinfa Ming 한국섬유공학회 2019 Fibers and polymers Vol.20 No.4

Silk fibroin/polypropylene (SF/PP) nonwoven fabrics were prepared via electrospinning method. The compositematerials combine the advantage of nanofibers with the high specific surface area and the strong affinity toward fluoride. SF/PP nonwoven fabrics were characterized, and the adsorption of fluoride was investigated. The results showed the diameter ofSF nanofibers increased from 950±37 nm to 2400±50 nm in nonwoven fabrics with the increase of SF concentration from5.0 wt% to 10 wt%. Before fluoride adsorption, no crystal particles were deposited on the surface of SF/PP nanofibers. Afteradsorption 5 min, only several white crystals were deposited. Within the increase of adsorption times from 10 min to 60 min,many larger crystals were grown. Fluoride ions were confirmed in crystals by EDS. From adsorption rate curve, the rate offluoride removal was rapid initially and slowed down gradually until it attained equilibrium. At the same time, a largefraction of fluoride ions was removed within 20 min of contact times in all the experimental various studied. Therefore, thisnonwoven fabric has a great potential as a novel adsorbent material for the fluoride removal from drinking water.

LncRNA KIAA0087 suppresses the progression of osteosarcoma by mediating the SOCS1/JAK2/STAT3 signaling pathway

Gong Haoli,Tao Ye,Xiao Sheng,Li Xin,Fang Ke,Wen Jie,He Pan,Zeng Ming 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Long noncoding RNAs (lncRNAs), widely expressed in mammalian cells, play pivotal roles in osteosarcoma (OS) progression. Nevertheless, the detailed molecular mechanisms of lncRNA KIAA0087 in OS remain obscure. Here, the roles of KIAA0087 in OS tumorigenesis were investigated. KIAA0087 and miR-411-3p levels were detected by RT-qPCR. Malignant properties were assessed by CCK-8, colony formation, flow cytometry, wound healing, and transwell assays. SOCS1, EMT, and JAK2/STAT3 pathway-related protein levels were measured by western blotting. Direct binding between miR-411-3p and KIAA0087/SOCS1 was validated by a dual-luciferase reporter, RIP, and FISH assays. In vivo growth and lung metastasis were evaluated in nude mice. The expression levels of SOCS1, Ki-67, E-cadherin, and N-cadherin in tumor tissues were measured by immunohistochemical staining. Downregulation of KIAA0087 and SOCS1 and upregulation of miR-411-3p were found in OS tissues and cells. Low expression of KIAA0087 was associated with a poor survival rate. Forced expression of KIAA0087 or miR-411-3p inhibition repressed the growth, migration, invasion, EMT, and activation of the JAK2/STAT3 pathway and triggered apoptosis of OS cells. However, the opposite results were found with KIAA0087 knockdown or miR-411-3p overexpression. Mechanistic experiments indicated that KIAA0087 enhanced SOCS1 expression to inactivate the JAK2/STAT3 pathway by sponging miR-411-3p. Rescue experiments revealed that the antitumor effects of KIAA0087 overexpression or miR-411-3p suppression were counteracted by miR-411-3p mimics or SOCS1 inhibition, respectively. Finally, in vivo tumor growth and lung metastasis were inhibited in KIAA0087-overexpressing or miR-411-3p-inhibited OS cells. In summary, the downregulation of KIAA0087 promotes the growth, metastasis, and EMT of OS by targeting the miR-411-3p-mediated SOCS1/JAK2/STAT3 pathway.

Simultaneous treatment with sorafenib and glucose restriction inhibits hepatocellular carcinoma in vitro and in vivo by impairing SIAH1-mediated mitophagy

Zhou Jing,Feng Ji,Wu Yong,Dai Hui-Qi,Zhu Guang-Zhi,Chen Pan-Hong,Wang Li-Ming,Lu Guang,Liao Xi-Wen,Lu Pei-Zhi,Su Wen-Jing,Hooi Shing Chuan,Ye Xin-Pin,Shen Han-Ming,Peng Tao,Lu Guo-Dong 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Transarterial chemoembolization (TACE) is the first-line treatment for unresectable intermediate-stage hepatocellular carcinoma (HCC). It is of high clinical significance to explore the synergistic effect of TACE with antiangiogenic inhibitors and the molecular mechanisms involved. This study determined that glucose, but not other analyzed nutrients, offered significant protection against cell death induced by sorafenib, as indicated by glucose deprivation sensitizing cells to sorafenib-induced cell death. Next, this synergistic effect was found to be specific to sorafenib, not to lenvatinib or the chemotherapeutic drugs cisplatin and doxorubicin. Mechanistically, sorafenib-induced mitophagy, as indicated by PINK1 accumulation, increased the phospho-poly-ubiquitination modification, accelerated mitochondrial membrane protein and mitochondrial DNA degradation, and increased the amount of mitochondrion-localized mKeima-Red engulfed by lysosomes. Among several E3 ubiquitin ligases tested, SIAH1 was found to be essential for inducing mitophagy; that is, SIAH1 silencing markedly repressed mitophagy and sensitized cells to sorafenib-induced death. Notably, the combined treatment of glucose restriction and sorafenib abolished ATP generation and mitophagy, which led to a high cell death rate. Oligomycin and antimycin, inhibitors of electron transport chain complexes, mimicked the synergistic effect of sorafenib with glucose restriction to promote cell death mediated via mitophagy inhibition. Finally, inhibition of the glucose transporter by canagliflozin (a clinically available drug used for type-II diabetes) effectively synergized with sorafenib to induce HCC cell death in vitro and to inhibit xenograft tumor growth in vivo. This study demonstrates that simultaneous treatment with sorafenib and glucose restriction is an effective approach to treat HCC, suggesting a promising combination strategy such as transarterial sorafenib-embolization (TASE) for the treatment of unresectable HCC.

Schisandrin B Improves the Hypothermic Preservation of Celsior Solution in Human Umbilical Cord Mesenchymal Stem Cells

Zhang Ying,Wang Peng,Jin Mei-xian,Zhou Ying-qi,Ye Liang,Zhu Xiao-juan,Li Hui-fang,Zhou Ming,Li Yang,Li Shao,Liang Kang-yan,Wang Yi,Gao Yi,Pan Ming-xin,Zhou Shu-qin,Peng Qing 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.3

BACKGROUND: Human umbilical cord mesenchymal stem cells (hUCMSCs) have emerged as promising therapy for immune and inflammatory diseases. However, how to maintain the activity and unique properties during cold storage and transportation is one of the key factors affecting the therapeutic efficiency of hUCMSCs. Schisandrin B (SchB) has many functions in cell protection as a natural medicine. In this study, we investigated the protective effects of SchB on the hypothermic preservation of hUCMSCs. METHODS: hUCMSCs were isolated from Wharton’s jelly. Subsequently, hUCMSCs were exposed to cold storage (4 C) and 24-h re-warming. After that, cells viability, surface markers, immunomodulatory effects, reactive oxygen species (ROS), mitochondrial integrity, apoptosis-related and antioxidant proteins expression level were evaluated. RESULTS: SchB significantly alleviated the cells injury and maintained unique properties such as differentiation potential, level of surface markers and immunomodulatory effects of hUCMSCs. The protective effects of SchB on hUCMSCs after hypothermic storage seemed associated with its inhibition of apoptosis and the anti-oxidative stress effect mediated by nuclear factor erythroid 2–related factor 2 signaling. CONCLUSION: These results demonstrate SchB could be used as an agent for hypothermic preservation of hUCMSCs.

Use of In Vivo-Induced Antigen Technology to Identify In Vivo-Expressed Genes of Campylobacter jejuni During Human Infection

( Yuan Qing Hu ),( Jin Lin Huang ),( Qiu Chun Li ),( Yu Wei Shang ),( Fang Zhe Ren ),( Yang Jiao ),( Zhi Cheng Liu ),( Zhi Ming Pan ),( Xin An Jiao ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.3

Campylobacter jejuni is a prevalent foodborne pathogen worldwide. Human infection by C. jejuni primarily arises from contaminated poultry meats. Genes expressed in vivo may play an important role in the pathogenicity of C. jejuni. We applied an immunoscreening method, in vivo-induced antigen technology (IVIAT), to identify in vivo-induced genes during human infection by C. jejuni. An inducible expression library of genomic proteins was constructed from sequenced C. jejuni NCTC 11168 and was then screened using adsorbed, pooled human sera obtained from clinical patients. We successfully identified 24 unique genes expressed in vivo. These genes were implicated in metabolism, molecular biosynthesis, genetic information processing, transport, and other processes. We selected six genes with different functions to compare their expression levels in vivo and in vitro using real-time RT-PCR. The results showed that the selected six genes were significantly upregulated in vivo but not in vitro. In short, these identified in vivo-induced genes may contribute to human infection of C. jejuni, some of which may be meaningful vaccine candidate antigens or diagnosis serologic markers for campylobacteriosis. IVIAT may present a significant and efficient method for understanding the pathogenicity mechanism of Campylobacter and for finding targets for its prevention and control.