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        • KCI등재후보SCIESCOPUS

          혼합배지와 지지체 특성에 의한 줄기세포 분화제어: 골연골 조직을 중심으로

          한정윤 ( Jeong Yoon Han ), 신지원 ( Ji Won Shin ), 박소희 ( So Hee Park ), 김동화 ( Dong Hwa Kim ), 김수향 ( Su Hyang Kim ), 강윤경 ( Yun Gyeong Kang ), 신정욱 ( Jung Woog Shin ) 한국조직공학과 재생의학회 2011 조직공학과 재생의학 Vol.8 No.2s

          This article investigated the potential of substrate characteristics for the control of differentiation of stem cells. For this, two distinctive materials, alginate and HA (hydroxiapatite) contained PCL, were selected. Utilizing rapid prototype (RP) technique we fabricated each type of scaffold. Mesenchymal stem cells (MSCs) derived from New Zealand White rabbits were suspended into alginate solution and the solution was plotted utilizing RP technique. The cocktail media which contain chondrogenic and osteogenic growth factors were used. The basal media were also used for comparison. During 14 days of experiments the samples were harvested four times. The results showed that MSCs tended to be differentiated into osteo- and chondrocyte-like cells when they were on PCL and in alginate scaffold, respectively even both of the two types of scaffolds were in basal media. These findings were more observable when the cocktail media were used. From this research we can stipulate that the characteristics of substrate materials should be considered along with biochemical reagents in the control of differentiation of stem cells. It is also recommended that the biomechanical factors, such as mechanical stimuli, should be considered in view of bio-mimetic environments.

        • KCI등재후보SCIESCOPUS

          치주염 및 임플란트 주위염 환자의 치은 조직에서 Real Time PCR을 이용한 Matrix Metalloproteinase (MMP)-2,9,12,13의 발현

          최봄 ( Bohm Choi ), 이태연 ( Tae Yeon Lee ), 윤현중 ( Hyun Joong Yoon ), 이원 ( Won Lee ) 한국조직공학과 재생의학회 2013 조직공학과 재생의학 Vol.10 No.1s

          Matrix metalloproteinases (MMPs) are known to be responsible factor in various pathological conditions including stomach ulcer, tumor metastasis, rheumatoid arthritis. In previous study, MMP-8 is kown as a key mediator in periodontitis but the exact role and functions of MMPs are still unclear. This study was to investigate the expressions of MMPs in gingival tissues from periodontally healthy individuals, chronic periodontitis patients, periimplantitis patients using real time polymerase chain reaction analysis. MMP-2, MMP-9, MMP-12, MMP-13 were analyzed in this study. mRNA level of MMP-2, MMP-9 were high in tissue extract isolated from advanced periodontitis compared to that of periodontally healthy individuals. Unlike to periodontitis, in peri-implantitis patients, only MMP-9 was slightly activated compared to periodontally healthy individuals. Otherwise, expression level of MMP- 12, MMP-13 in periodontally unhealthy patients was extremely low. In RNA level, expression profile of MMPs between periodontitis and peri-implantitis was similar but there are some different aspects in individual expression. It can imply that there be the similar mechanisms and relation between two diseases but different regulatory mechanisms can be exsist.

        • KCI등재후보

          치주염 및 임플란트 주위염 환자의 치은 조직에서 Real Time PCR을 이용한 Matrix Metalloproteinase (MMP)-2,9,12,13의 발현

          최봄,이태연,윤현중,이원 한국조직공학과 재생의학회 2013 조직공학과 재생의학 Vol.10 No.1

          Matrix metalloproteinases (MMPs) are known to be responsible factor in various pathological conditions including stomach ulcer, tumor metastasis, rheumatoid arthritis. In previous study, MMP-8 is kown as a key mediator in periodontitis but the exact role and functions of MMPs are still unclear. This study was to investigate the expressions of MMPs in gingival tissues from periodontally healthy individuals, chronic periodontitis patients, periimplantitis patients using real time polymerase chain reaction analysis. MMP-2, MMP-9, MMP-12, MMP-13 were analyzed in this study. mRNA level of MMP-2, MMP-9 were high in tissue extract isolated from advanced periodontitis compared to that of periodontally healthy individuals. Unlike to periodontitis, in peri-implantitis patients, only MMP-9 was slightly activated compared to periodontally healthy individuals. Otherwise, expression level of MMP-12, MMP-13 in periodontally unhealthy patients was extremely low. In RNA level, expression profile of MMPs between periodontitis and peri-implantitis was similar but there are some different aspects in individual expression. It can imply that there be the similar mechanisms and relation between two diseases but different regulatory mechanisms can be exsist.

        • KCI등재후보SCIESCOPUS

          안면부의 조직공학적 재생을 위한 발생영역 수복에 대한 고찰

          박정민 ( Jung Min Park ), 서미현 ( Mi Hyun Seo ), 김성민 ( Soung Min Kim ), 김선아 ( Seon Ah Kim ), 명훈 ( Hoon Myoung ), 이종호 ( Jong Ho Lee ) 한국조직공학과 재생의학회 2013 조직공학과 재생의학 Vol.10 No.1s

          In growing child, corrective surgery for congenital malformation can cause complication and problems, such as repetitive relapse and growth disturbance over time. These are because the biologic rationale of the procedure is incorrect, and the anatomic pattern of relapse points an accusatory finger at the pathology. Recent updates in surgical approach for the clefting are focused on facial development and origin of clefting. Successful treatment of congenital craniofacial defect can be acquired by understanding of developmental processes. The developmental field repair (DFR) is based on the neuromeric model, which propose facial tissues are originated from embryonic nervous system. This concept consider the identification and reassignment specific developemental field. Goals of DFR surgery are 1) resolution of all pathologic processes of clefting, 2) dissection following the embryonic separation plane, 3) preservation of vascular supply for alveolar mucoperiosteum, 4) primary unification of alveolus, 5) reassignment of all developmental field to correct relation. Therefore, understanding of developmental field theory must be proceeded surgical approach. This concepts are based on reconstruction of deformed tissues following embryonic origin, which are able to be functional and anatomical repair. Therefore, in this article the author reviewed the developmental neuromeric theory, and the developmental field repair concept based on this neuromeric model.

        • KCI등재SCIESCOPUS
        • KCI등재후보SCIESCOPUS

          골재생을 위한 세포치료

          임군일 ( Gun Il Im ) 한국조직공학과 재생의학회 2010 조직공학과 재생의학 Vol.7 No.5

          골은 우리 몸에서 손상시 자가조직으로 재생되는 조직이지만 광범위한 골 결손의 경우나 일부 골질환에 있어서는 골 수복에 어려움이 있으며 전신적으로 골형성에 문제가 있는 유전질환에서 세포치료의 필요성이 받아들여지고 있다. 골재생을 위한 세포치료는 주로 골수기원의 간엽줄기세포를 이용하며 그대로 분리해 사용하거나 시험관내에서 증식, 분화시켜 사용하는 것이 모두 가능하다. 현재 여러 임상시도가 국소치료와 전신치료에서 이루어 지고 있으나 아직 보편화된 치료로 자리를 잡고 있지는 못하다. 골수 줄기세포의 양이 제한되어 있다는 점을 고려하면 골수외에도 지방줄기세포등 다른 세포원을 찾고 이들에 유 전자전달 등을 통하여 골형성 효율을 높이는 것에 대한 연구도 필요하다고 사료되며 현재 주목을 받고 있 는 유도 다분화능 만능줄기세포의 안전성이 담보된다면 이 세포의 골재생 세포치료에 이용가능성도 타진 해야 할 것으로 사료된다. 본 총설에서는 현재까지 임상에 적용된 세포치료의 현황을 적용 질환 중심으로 정리하여 소개하고자 한다.

        • KCI등재후보

          혼합배지와 지지체 특성에 의한 줄기세포 분화제어 : 골연골 조직을 중심으로

          한정윤,신지원,박소희,김동화,김수향,강윤경,신정욱 한국조직공학과 재생의학회 2011 조직공학과 재생의학 Vol.8 No.4

          This article investigated the potential of substrate characteristics for the control of differentiation of stem cells. For this, two distinctive materials, alginate and HA (hydroxiapatite) contained PCL, were selected. Utilizing rapid prototype (RP) technique we fabricated each type of scaffold. Mesenchymal stem cells (MSCs) derived from New Zealand White rabbits were suspended into alginate solution and the solution was plotted utilizing RP technique. The cocktail media which contain chondrogenic and osteogenic growth factors were used. The basal media were also used for comparison. During 14 days of experiments the samples were harvested four times. The results showed that MSCs tended to be differentiated into osteo- and chondrocyte-like cells when they were on PCL and in alginate scaffold, respectively even both of the two types of scaffolds were in basal media. These findings were more observable when the cocktail media were used. From this research we can stipulate that the characteristics of substrate materials should be considered along with biochemical reagents in the control of differentiation of stem cells. It is also recommended that the biomechanical factors, such as mechanical stimuli, should be considered in view of bio-mimetic environments.

        • KCI등재후보

          안면부의 조직공학적 재생을 위한 발생영역 수복에 대한 고찰

          박정민,서미현,김선아,명훈,이종호,김성민 한국조직공학과 재생의학회 2013 조직공학과 재생의학 Vol.10 No.1

          In growing child, corrective surgery for congenital malformation can cause complication and problems,such as repetitive relapse and growth disturbance over time. These are because the biologic rationale of the procedure is incorrect, and the anatomic pattern of relapse points an accusatory finger at the pathology. Recent updates in surgical approach for the clefting are focused on facial development and origin of clefting. Successful treatment of congenital craniofacial defect can be acquired by understanding of developmental processes. The developmental field repair (DFR) is based on the neuromeric model, which propose facial tissues are originated from embryonic nervous system. This concept consider the identification and reassignment specific developemental field. Goals of DFR surgery are 1)resolution of all pathologic processes of clefting, 2) dissection following the embryonic separation plane, 3) preservation of vascular supply for alveolar mucoperiosteum, 4) primary unification of alveolus, 5) reassignment of all developmental field to correct relation. Therefore, understanding of developmental field theory must be proceeded surgical approach. This concepts are based on reconstruction of deformed tissues following embryonic origin, which are able to be functional and anatomical repair. Therefore, in this article the author reviewed the developmental neuromeric theory,and the developmental field repair concept based on this neuromeric model.

        • 토끼 모델에서 탈미네랄화된 골분을 함유한 PLGA 지지체를 이용한 조직공학적 골 재생

          장지욱 ( Ji Wook Jang ), 박기숙 ( Ki Suk Park ), 김순희 ( Soon Hee Kim ), 박종수 ( Chong Soo Park ), 김문석 ( Moon Suk Kim ), 한창환 ( Chang Whan Han ), 이종문 ( John M. Rhee ), 강길선 ( Gil Son Khang ), 이해방 ( Hai Bang Lee ) 한국조직공학과 재생의학회 2005 조직공학과 재생의학 Vol.2 No.1

          Demineralized bone particle (DBP), one of the significant natural bioactive materials, has a powerful inducer of new bone growth. It has been recognized that DBP contains many kinds of osteogenic and chondrogenic cytokines as bone morphogenic protein (BMP). BMP acts as local mitogen to stimulate proliferation of mesenchymal stem cell. Bone marrow stromal cell (BMSCs) can be differentiated in culture into osteoblasts, chondrocytes, and myoblasts with controlling of the environment of cell growth. In this study, we developed the DBP loaded poly(L-lactide-co-glycolide) (PLGA) scaffolds for the possibility of the application of the tissue engineered bone. PLGA/DBP scaffolds were prepared by solvent casting/salt leaching method and characterized by porosimeter, scanning electron microscopy, and differential scanning calorimetry. BMSCs were isolated from femur of New Zealand white rabbit and cultured with osteo-medium for osteogenic differentiation. DBP impregnated PLGA scaffolds with BMSCs were implanted into the head and femur of New Zealand white rabbit to observe the effect of DBP on the osteoinduction compared with control scaffolds. Thin sections were cut from paraffin embedded tissues and histological sections were stained hematoxylin & eosin and safranin-O. It can be observed that the porosity was above 94.6% and the pore size was above 69.8 ?m. In vivo study, we could observe that similar to bone tissue region in PLGA/BMSCs and PLGA/BMSCs/DBP groups, but bone tissue region did not occur almost in control scaffolds. From these results, it seems that DBP play an important role for bone induction on BMSCs.

        • KCI등재후보SCIESCOPUS

          형상이 제어된 골 조직 재생용 3차원 지지체를 적용한 새로운 형태의 Perfusion Culture

          이시우 ( Shi Woo Lee ), 허수진 ( Su Jin Heo ), 장지연 ( Ji Yeon Jang ), 정재영 ( Jae Young Jeong ), 김수향 ( Su Hyang Kim ), 박수아 ( Su A Park ), 전은수 ( Eun Su Jeon ), 신정욱 ( Jung Woog Shin ) 한국조직공학과 재생의학회 2010 조직공학과 재생의학 Vol.7 No.1

          The objective of this study was to investigate the efficacy of a newly designed perfusion bioreactor which provides flow-induced mechanical stimulation on the cells residing in the intra-morphology controllable scaffolds. For this, we fabricated scaffolds composed of poly e-caprolactone (PCL) and micro-sized hydroxyapatite (HA) particles using rapid-prototyping process. Also a new bioreactor system for perfusion culture was designed and developed. For the analyses of cellular responses of bone-tissue related cells cultured in the perfusion bioreactor system, various biological assays were performed such as MTT test, DNA content measurement, FE-SEM and live/dead staining. The cells used in this study were MG-63 (human osteoblast-like cell line) and mesenchymal stem cells from New Zealand white rabbits. Our results showed that the cells cultured by the perfusion bioreactor resulted in higher proliferation rate and mineralization of extracellular matrices than those cultured in static culture. From this study, we could confirm the potentials of 1) a newly developed perfusion bioreactor, 2) intra-morphology controllable scaffolds composed of PCL and HA particle and 3) the combination of the suggested scaffolds and perfusion culturing system in relation to bone tissue engineering.

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