GC-ECD를 이용한 유기염소계 및 Pyrethroid계 농약 동시 분석법

김우성,이선화,김재이,정지윤,이명자,박영채,이영자,정성욱,이봉헌,박흥재 한국환경과학회 2003 한국환경과학회지 Vol.12 No.4

Pesticides were extracted from samples with 70% acetone and methylene chloride in order, and then cleaned up via open-column chromatography apparatus packed with florisil, and finally analyzed simultaneously the organochlorine and pyrethroid pesticides using GC(ECD). An ultra-2 fused silica capillary column was used to separate and identify the products. The resolution between the last isomeric peak of cypermethrin(59.987min) and the first isomeric peak of flucythrinate(60.043min) was not satisfactory. The last isomeric peak of fenvalerate(62.344min) and the first isomeric peak of fluvalinate(62.397min) were overlapped. Recoveries of soybean sample for the most pesticides were 73.3% to 102.4%. Detection limits were between 0.004 and 0.063 ㎍/㎎ when this method was used.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1994)

김재홍,안진균,정성재,김영태,김중환,김시영,이석종,이홍렬,서호석,김경훈,권혁진,정우권,고우석,이용석,안필수,오준규,오용섭 대한화학요법학회 1996 대한화학요법학회지 Vol.14 No.1

Background : In recent years gonorrhoea has been panendemic and remains one of the most common Sexually Transmitted Diseases in the world, especially in developing countries. Objective & Method : For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria Gonorrhoeae(PPNG), Ive have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results : In 1994, 168 strains of N. gonorrhoeae were isolated, among which 109 (64,9% ) were PPNG. Conclustion : Our results suggests that the prevalence of PPNG in Seoul is still increasing.

微生物에 의한 石油內 硫黃分除去에 관한 硏究 : Ⅳ. 脫黃菌의 特性에 대한 生理學的 硏究 Ⅳ. Physiological Studies on Characters of Sulfur-oxidizing Bacteria

李敏載,河永七,李光雄,邊宇玄 서울대학교 1975 서울대학교 論文集 Vol.25 No.-

Serveral physiological characterstics of sulfur­oxidizing bacteria such as Thiobacillus thiooxidans and T. concretivorus are described. 1) Effect of pH on inhibitory function of pyruvate. The rate of oxygen uptake was reduced in accordance with decrease of pH. And on the other hand inhibition rate of pyruvate was more severe in lower pH than in higher pH. High concentration of hydrogen ion seemed to accelerate inhibitory function of pyruvate. Limiting concentration of pyruvate which absolutely inhibit oxygen uptake was lowered with time. In O time, 10^-2M of pyruvate absolutely inhibited oxygen uptake in pH 5. After 24 hours limiting concentration was lowered to 10^-3M. 2) Effect of organic compounds on the oxygen uptake of T. concretivorns. Effect of glucose, fructose, xylose, glutamate, succinate, malate, glycine, lactate, acetate, pyruvate, citrate, formate, and cis­aconitate on thiosulfate oxidation and availability of these compounds as sloe source of energy by T. concretivorus were observed. A 0.5% concentration of malate and glycine accelerated thiosulfate oxidation almost react somewhat inhibitory. Pyruvate and citrate inhibited thiosulfate oxidation. In thiosulfate­free medium, organic compounds except formate and pyruvate affected no significant influences on oxygen uptake. 3) Enzyme assay. Of enzymes concerned TCA cycle and glycolysis, T. concretivorus had most of those enzyme activities even though they were low. Activities of hexokinase and succinic dehydrogenase were somewhat high and that of aconitase was very low on the contrary. 4) Optimum condition of mass culture of sulfur oxidizing bacteria. In a large jar fermenter opimum conditions including agitations speed, volume of supplying gases, and constitution rate of oxygen and carbon dioxide, etc. were experimented. The sulfur­oxidier, T. thiooxidans was grown most effectively under the condition of autom­atically controlled pH of 1.0, and with the aeration of oxygen and carbon dioxide mixture (5 : 1) at the flow rate of 0.05 vvm, and at the agitation velocity of 200 rpm in this laboratory scale fermenter.

감마선조사 생쥐의 폐 및 심장조직중의 Superoxide Dimutase 활성도에 대한 연구

이상협,전현우,김우제,양종대,차종희 朝鮮大學校 附設 醫學硏究所 1986 The Medical Journal of Chosun University Vol.11 No.1

The change of cytosolic superoxide dismutase (E.C.1.15.1.1. ) activities in the lung and heart of mice irradiated with a single dose of gamma irradiation 400 rads for the whole body was examined. The specific activities of superoxide dismutase in cytosol fraction of lung and heart were decreased to lowest levels on 7 day after irradiation and the activities of superoxide dismutase per gram of the organ tissues were decreased to lowest level on 21 day after irradiation. Also the total activities of superoxide dismutase per lung and heart were decreased to lowest level on 7 day after irradiation, It is concluded that the superoxide dismutase activity in cytosol of heart is more decreased than the activity in cytosol of lung after irradiation.

Ortho-Chlorobenzylidene Malononitrile(CS) 이 흰쥐 피부조직에 미치는 조직학적 영향에 관한 연구

이우용,최봉근,김잉곤,엄기일,정호삼,정호삼,류재만 한양대학교 의과대학 1998 한양의대 학술지 Vol.18 No.1

This study was undertaken to describe the fine structural changes of cytoplasmic organelles in epidermal cells, and the changes of volume and localization of collagen type Ⅰ in the dermis of rat skin by CS(Ortho-chlorobenzylidene malononitrile) application. The experimental animals, 100∼150gm of body weight rats(Sprague-Dawley strain), were sacrificed at 12 hours, 24 hours, 3 day and 5 day after CS application on the hair-shaved back skin. The specimens were prepared for ultrastructural findings, ultrathin sections were stained with uranyl acetate and lead citrate double stain and observed with Hitachi-600 electron microscrope. The others were incubated with rabbit anti rat type Ⅰ collagen antibody as primary antibody, and Goat anti rabbit IgG antibodies as secondary antibody And advidin-biotin-conjugated(ABC) peroxidase procedure was used. All of specimens for immune reactions were observed with light microscope. The results obtained were as follows. 1 Keratinocytes appeared fully necrotized after 3 days of CS application. The epidermis showed degeneration of basal lamina, enlargement of intercellular spaces and necrotic keratinocytes with vacuole and vesicles. And large keratohyalin granules were seen in the shrunken epidermal cells. 2 At 3 or 5 day after CS treatment, the normal cytoplasmic organelles of keratinocytes began to appear on the basal and prickel cells. 3 At 12 hour an 24 hour after CS treatment. The immune reaction of type Ⅰ collagen showed weak reaction in reticular layer of dermis. But at 3 day and 5 day after CS treatment, moderate immune reaction were seen in throughout the dermis. These results suggest that CS application to rak skin may induce changes of keratinocytes and reductive changes of type Ⅰ collagen in the dermis.

폴리머 콘크리트 복합체를 이용한 교통안전 시설물 개발

이명호,이재철,이승우,서장일,박세희 강원대학교 부설 석재복합신소재 제품연구센터 2004 석재연 논문집 Vol.9 No.-

폴리머 콘크리트 복합체를 이용하여 악천후에도 시인성이 좋고 주위의 경관과 어울리는 자동점멸 표지병을 개발하였다. 아날로그 회로에 비해 설계상의 난해한 점이 있으나 상대적으로 간편한 디지털 회로를 사용하여 자동점멸 회로를 구성하였으며, 작업의 능률을 높이고 개발비를 줄이기 위하여 OrCAD를 사용한 Simulation을 실시후 회로를 구성하였다. 표지병의 시공시와 같은 환경에서의 실험에서는 디지털회로의 작은 전류와 표지병간의 거리, 전선의 저항으로 인하여 클럭 및 클리어 신호에 전압강하와 상승/하강 시간의 증가가 나타났다. 표지병은 전자회로와 함께 설치되어야 하므로 방수성을 고려하여 표지병과 전자회로를 일체형으로 설계하였다. 실제 시공시의 환경에서 시행한 실험에서 나타난 문제점을 검토하였으며, 향후 연구방향에 관하여 고찰하였다. Using polymer concrete composite, the automatic-on/off road marker that is well distinguished in a bad weather condition of rainy or foggy day and is well harmonized with. Digital circuit that is hard to design but simpler than an analog circuit is used in designing the automatic-on/off circuit, and it order to raise the working efficiency and lower the development cost, OrCAD, a simulation tool of electric circuit, was used in simulating the circuit. In an experiment conducted under the circumstance of practical application, voltage drop and raising/lowering time increment in the clock and the clear signals are occurred because of small current of digital circuit and resistance of electrical line. Because that the road marker is assembled with the digital circuit, it is designed as one assembled unit by considering water proof. Some problems occurred in the experiment and the future work are discussed.

도암호의 수질과 어류군집 특성 연구

이상하,최재석,이광열,장영수,임인수,허우명,김재구,김범철 강원대학교 부속 환경연구소 2005 Journal of the Environment Vol.2 No.-

To investigate nutrient dynamics of the lake and fish community, both water and fish samples were collected at three different areas (dam site, upper inflowing and downstream of lake outfow) for one year from Sep.2004 to Aug.2005 During the experimental periods, mean concentration of chl, a in epilimnetic layer (0-5m) was 18.5mg^(-3) and transparency ranged from 0.3m to 2.4m, respectively. In nutrient, mean concentrations of TP and TN were 111 mgP m^(-3) and 4.4 mgN L^(-1), respectively. Based on the water quality standard suggested by U.S.EPA(*1976), the trophic state of Lake Doam was classified as eutrophic state. Total number of fish collected in Lake Doam was 9,600 indiveduals in 26 species of 6 family. Sixteen of Korean endemic soecies including H. mylodon, A. signifer, P. tenuicorpa occurred. O. mykiss as introduced fish was also found in Lake Doam. Dominant and subdominant species were P. herzi and Z. platypus that took about 34.6% and 22.5% of total fish composition, respectively. Six species including A. signifer. P. tenuicorpa. and R. kumgangensis were rare in Lake Doam. Pelagic typw of figh commonly occurred at upper regions, whereas benthic type was mainly domenated at downstream area. Therefore, pollution source inflowed from the upper regions did not seriously affect downstream area (Songcheon water system) due to the role of dam as pollutant barrier. In addition high appearance of Comat type of fish that si hybrid between gold fish (C.auratus) and C. auratus was found in the lake, It was unclear the reasons that high prorortion of mutant species apperared in the lake. Further more researches are required in this area.

완전 탈구된 치아의 재식에 대한 증례보고

이제호,최병재,손흥규,조우성 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.3

The treatment of complete avulsed teeth due to traumatic injuries is replantation procedure. The major complication of replantation are pulp necrosis, ankylosis, and root resorption. It is important to maintain the vitality of periodontal ligament to minimize the complications. The purpose of this case report is to consider the facts that affect the success rate and the prognosis of complete avulsed teeth replantation. All of these cases had different conditions and transport media. The followng results were observed : 1. The successive treatment of complete avulsed teeth requires the maintainance of vitality of periodontal ligament. 2. The complication of replantation are inflammatory root resorption and ankylosis and in such case, proper endodontic treatment using calcium hydroxide medications and periodic observations are needed. 3. In case of delayed replantation, the use of fluoride and root planning procedure can help to achieve better result. 4. It is more esthetic and functional to maintain the avulsed tooth in mouth.

텅스텐 소결에 미치는 Cu 첨가효과

이재성,박상우 한양대학교 공학기술연구소 1993 공학기술논문집 Vol.2 No.1

The influence of Cu addition on sintering behaviour of W powder compacts was investigated. The addition of Cu (0-5wt%) to fine W powder(1.02㎛) was done in the form of CuC12H2O for improving mixing homogeneity. After reduction W-Cu powders were compacted and sintered at 1000∼1400℃ in hydrogen atmosphere for 1 h. The densification process of W-Cu compacts strongly depended on Cu content. Below 1%Cu the compacts had the same temperature dependence as pure W compact. Volume shrinkage was abruptly decreased by addition of 0.1%Cu and then increased with increasing Cu content at all temperatures. Such negative effect is thought to result from the lowering of W grain boundary self diffusion process by Cu segregants. Remarkable growth of W particles occured in the W-5%Cu sintered at 1400℃, which might be considerably affected by Ostwald ripening process beside particle coalescence.

카드뮴 중독 흰쥐에 대한 Noninvasive Biomarker로서의 요중 Ascorbic Acid에 관한 연구

이용우,김용호,안승주,류재두 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1997 Journal of biomedical laboratory sciences Vol.3 No.2

본 연구는 카드뮴의 이용 및 환경오염의 증가로 일어날 수 있는 카드뮴 중독에 대한 새로운 생물학적 지표로서의 요중 ascorbic acid를 평가하기 위하여 실험적으로 중독시킨 Sprague-Dawley종의 흰쥐를 이용하여 요중 ascorbic acid측정, 신장의 조직병리학적 조사 및 생화학적 간 기능 검사를 실시하였다. 측정된 요중 ascorbic acid농도의 전 실험기간 (50일) 평균치는 실험군Ⅰ(카드뮴 100ppm)이 214.0㎎/dl, 실험군Ⅱ(카드뮴 200ppm)가 254.3㎎/dl로 대조군 9.0㎎/dl에 비해 각각 24배 및 28배의 증가를 보였다. 조직병리학적 소견으로는 신장의 근위 세뇨관에서 단백뇨의 원인으로 추정되는 호산성 초자양 물질이 관찰되어 신장의 손상이 있었고, 생화학적 분석에서 실험군Ⅰ에서 AST, ALT의 수치가 대조군의 143㎎/dl, 50㎎/dl에 비해 각각 199㎎/dl, 88㎎/dl, 실험군Ⅱ가 270㎎/dl,226㎎/dl로 나타나 간 기능의 손상이 있었음을 알 수 있었다. 이상의 결과로 실험동물이 카드뮴에 노출됨에 따라 그 아만성 독성으로서 간장 및 신장 기능의 손상이 있었고 또한 요중의 ascorbic acid가 현저하게 증가되어 요중 ascorbic acid가 실험동물의 카드뮴 노출에 대한 noninvasive진단 지표로서 활용될 수 있을 것으로 기대된다. This study was planned to evaluate the urinary ascorbic acid as a new bilolgical marker for the intoxication of cadmium, which could possibly be driven by its increased utilization and environmental pollution. In order to meet this goal, we have performed measurement of urinary ascorbic acid concentration, histopathological examination of the kidney, and biochemical test for the liver function using cadmium-intoxicated rats by oral administration. The average concentrations of urinary ascorbic acid in the CdCl₂-treated rats were 214.0㎎/dl for 100ppm group and 254.3㎎/dl for 200ppm group during experimental period of 50 days. These levels are 24 and 28 times higher than one in the control group (9.0㎎/dl), respectively. Ultrastructural study showed the eosinophilic hyaline cast and focal effacement, fusion in the renal tubules, as well as loss of foot processes on the glomerular epithelial cells. These results suggested that cadmium may be responsible for renal glomerular injury. The blood levels of AST, ALT and LDH in the treated groups (199 IU/I, 88 IU/I, 1190 U/I for the 100 ppm group and 270 IU/I, 226 IU/I, 760 U/I for the 200 ppm group) were higher than ones in the control group(143 IU/I, 50 IU/I, 334 U/I). These results indicated the cadmium induced the damage of liver function. In conclusion, the administration of cadmium showed a remarkable increase of urinary ascorbic acid with renal and hepatic damage. Therefore, it is expected that measurement of urinary ascorbic acid would be an powerful method as a noninvasive biomarker for cadmium intoxication.