Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

한국인의 사회 재적응률 척도에 관한 조사

조주연,민성삼,이영진,조동영,이혜리,윤방부 대한가정의학회 1985 Korean Journal of Family Medicine Vol.6 No.12

서울시 대기오염과 일별 사망의 상관성에 관한 시계열적 연구 (1998~2001년)

조용성,이종태,김윤신,홍승철,김호,하은희,박혜숙,이보은 한국대기환경학회 2003 한국대기환경학회지 Vol.19 No.6

This study was performed to examine the relationship between air pollution exposure and mortality in Seoul for the years of 1998~2001I. Daily counts of death were analyzed by general additive Poisson model, with adjustment for effects of seasonal trend, air temperature, humidity, and day of the week as confounders in a nonparametric approach. Daily death counts were associated with CO (current day), 0₃(current day), PM_(10) (current day), NO₂, (1 day before), SO₂, (1 day before). Increase of 41.711㎍/㎥ (interquartile range) in PM_(10) was associated with 1.3% (95% CI = 0.7~1.9%) increase in the daily number of death. 0₃concentrations resulted in an increased risk of 1.3% for 23.86 ppb in all-aged mortality [RR = 1.013 (1.004-1.023)]. This effect was greater in children (less than 15 aged) and elderly (more than 65 aged). After ozone level exceeds 25 ppb, the dose-response relationship between mortality and ozone was almost linear. We concluded that Seoul had 1~5% increase in mortality in association with IQR (interquartile range) in air pollutants. Daily variations in air pollution within the range currently occurring in Seoul might have an adverse effect on daily mortality. These findings also support the hypothesis that air pollution, at levels below the current ambient air quality standards of Korea, is harmful to sensitive subjects, such as children or elderly.

사업장근로자의 구강보건인식 및 실천에 관한 연구

조성숙,최미혜 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1

This study is to investigate the recognition of oral by workers in enterprising place and the degree of their care. The subject of investigation is 198workers at three companies in Kyunggi-Do. The duration to collect the survey takes from September 1 to September 30, 2000. The means for survey is to prove the reliability and property. For the method of analysis, it used frequency, percentage, average, standard deviation, t-test, ANOVA and Duncan's multiple comparison test. The conclusion is as followings. Firstly, regarding the general characteristics, men from all the subject, 198workers for this survey are 73.2%. Regarding ages, 20∼29years or less is 49%. 30∼39years or less is 33.8%. Regarding the school carrier, the graduate from high school is 40.9% and collage is 38.4%. Accordingly, the degree of school carrier was high. People who work for research is 34.8%. Regarding the degree of their economy, middle is 75.3%. Secondly, regarding the recognition of oral health, alveolitis prevention effect was high but decayed tooth effect was low. And the needs of dental surgery were low. Thirdly, reviewing the action of oral health, the recognition of necessity and importance of scaling was low. Most of the workers dont get scaling their teeth regularly. Although they recognized it is necessary to get regular oral examine for oral health, the grade to do actually was low. Fourthly, on the basis of general feature, considering the recognition of oral health, 20∼29years workers were got a high grade. Manufacturing workers was lower than official and technical service workers. In the aspect of the level of economy, middle class is higher than low(p<.05). Reviewing the action, women are higher than men. The more age and the level of economy, the more action become(p<.05).

무우종자의 발아촉진에 관한 연구

조성희,성찬기 中央大學校 自然科學硏究所 1987 自然科學硏究所 論文集 Vol.1 No.-

The changes of some components in the cell and germination rate of Raphonus Sativus L. seeds that were treated with GA₃, KINETIN and NAA were observed. The results were follows: 1. The content of soluble protein was increased in homogenate and cytosolic fractions of GA₃, and KINETIN treated seeds compared with one of control. 2. The content of glucose was increased in homoginate and cytosoli fractions of GA₃and KINETIN treated seeds compared with one of control on the half and the first day. 3. The activity of trehalase was increased in homogenate and cytosolic fractions of seeds which treated with GA₃and KINETIN compared with one of control on the first day. 4. The ATPase did not changed in all fractions of the untreated and stimulant treated seeds, during germination stages. 5. The germination rate was remarkedly increased by continuous soak treatment of GA₃and KINETIN on the first and second days but in the case of NAA treatment, it was very smiliar to control.

Gα^(h)-단백질에 의해 활성화되는 Phospholipase C 이소효소의 확인

조강호,박권식,채규영,한기정,김형석,김형일,윤혜영,권년수,이희성,백광진 중앙대학교 의과대학 의과학연구소 2000 中央醫大誌 Vol.25 No.4

경주마 생체시료에서의 난드로론과 대사체의 검출 및 정량

김성진,조성희 중앙대학교 기초과학연구소 2000 基礎科學硏究所 論文集 Vol.14 No.-

In veterinary practice, anabolic steroid the nandrolone have been widely used in androgen therapy and illegal treatment. So current study was undertaken to quantify the steroids (nandrolone and its major metabolite estranediol) after administration and develope the screening method for the detection of anabolic steroid residues in equine body fluids. In the equine plasma, after extracted by disposable C18 Sep-pak cartridge with elution solvent, the organic layer was evaporated to dryness. The residue was subjected to LC/MS/MS analysis. After LC/MS/MS analysis, liquid chromatographic retention time data and mass spectral data were used for the detection and identification of nandrolone. The good quality control and recovery range were obtained for the present method. In the urine, after enzyme hydrolysis, the urine was extracted by disposable C18 Sep-pak cartridge and diethyl ether. After the ether layer was evaporated to dryness, the residue was subjected to derivatization prodedure and GC/MS analysis. After GC/MS analysis, both gas chromatographic retention time and mass spectral data were used for the detection and identification of estranediol. Also good quality-control data and recovery range were obtained for the present method. Using these methods, the concentrations of nandrolone and estranediol were determined in equine plasma and urine. Excretion profile of nandrolone presents the rapid increase up to maximum values at Days 4 after dosing and the slow regression in plasma and estrandiol major urinary metabolite of nandrone also presents increase up to maximum values at Days 5 after dosing and slow regression in urine.

텔레커뮤팅(재택근무)의 특성과 교통수요의 변화

조성혜 誠信女子大學校 韓國地理硏究所 1998 應用地理 Vol.- No.21

The emergence of new information technologies and recent advances in existing telecommunication technologies significantly affects our daily lives. This paper presents what changes then will our lives undergo in the near future if we accept telecommuting, a new system born as a result of the telecommunication technology development. Once workplaces become identical to residences, many changes are expected to occur in the daily lives of individuals, such as the reduced commuting time, securement of spare time, and arbitrariness related to time distribution. Of such many changes, what connection will telecommuting have in deciding private travel demand? What characteristics will the time and space, required by the daily lives of the telecommuters leading the informatized society, carry? It has been confirmed that the Korean telecommuters form unique time and space patterns which are distinctive from those of persons going to existing offices. With respect to commuting frequency, in particular, a pattern entirely different from that of foreign countries has been noted, indicating travel can be reduced to an extent capable of contributing to the solving of traffic problems. Even the trip chaining is entirely different from those of common employees. However, introduction of telecommuting does not seem so simple. In order to reflect telecommuting in future traffic policies and city planning, we should approach with an understanding that travel demand is generated by the individuals in line with their daily activities. As future projects, therefore, detailed quantitative researches should be carried out in relation to the construction of modelling, such as the estimation of time it takes for the telecommuting to be reflected in traffic policies and city planning, detailed surveys and analysis with respect to time spent by the people in the information-oriented society, and the surveys and analysis of the space pattern connected with the time usage.

Clostridium difficile 장염에 의한 속발성 유전분증에서 발현된 독성 장염

조형원,정혜경,강현주,이윤표,강혜원,심기남,정성애 Ewha Womans University School of Medicine 2014 EMJ (Ewha medical journal) Vol.37 No.1

Amyloidosis is characterized by extracellular deposition of protein fibrils in one or multiple organs. AA amyloidosis is secondarily occurred to be related with chronic infections or inflammatory diseases. We report a 67-year-old man suffered from secondary AA amyloidosis related with chronic Clostridium difficile colitis after repeated total hip replacement surgery. Infection control is the most important treatment of AA amyloidosis secondary to chronic infection. However, the patient’s C. difficile colitis was not controlled well, eventually toxic megacolon with sepsis was developed. Consequently, he had to take total colectomy, but he expired with multi-organ failures. We suggested that early surgical procedure might be one option for intractable C. difficile colitis complicated with secondary amyloidosis.

MBTI 성격유형에 따른 청소년의 또래 우정의 질 및 교사 친밀도

조혜영,성영혜 숙명여자대학교 아동연구센터 2002 兒童硏究 Vol.15 No.2

The purpose of this study is to investigate how is the difference at the quality of friendship of age and the intimacy degree of student-teacher according to the teenagers' sex distinction, and also, how is the difference at the quality of friendship of age and the intimacy degree of teacher according to the functional, dispositional, and sixteen nature types by MBTI(Myers-Briggs Type Indicator) test. Moreover, it is to help teachers and advisers at school which teenagers spend a lot of time, at consultation office, and at other institutions recognize the difference according to the sex distinction and the nature type, and also to help them improve the relationship between teenagers and teachers. The subject of this study were 560 students ( boys:308, girls:252) of academic high school located in Seoul and Busan. As a study tool, the MBTI Questionnaire was used to inspect the nature type of teenagers, the Friendship Quality Questionnaire:FQQ which was made by Parker and Asher was used to measure the friendship of age, and the questionnaire for the inspection of intimacy degree of student-teacher was used. For the collected data, the SAS pc program was conducted and according to the study problems, X2-test and the one-way ANOVA were carried out. To see the general characteristics, the frequency and the percentage were used and to see the quality of friendship of age and the intimacy degree of teacher, the mean and standard deviation were used. And then, for the post-verification, the independent t-test was also carried out. To see the quality of friendship of age according to the nature type of teenagers and to see the intimacy degree of teacher, the mean and the standard deviation were used, and then, the Duncan verification was conducted for the post verification. To summarize the results according to the study problems are as follows; First, there was a meaningful difference at the quality of friendship of age according to the sex distinction of teenagers (It I = 8.19, p<.001). That is, girls recognized more highly the friendship of age than boys, and in the intimacy degree of teacher, there was no difference between boys and girls. Second, there was no difference at the quality of friendship of age according to the functional nature type of teenagers, and also, there was no difference in the intimacy degree of teacher. Third, at the quality of friendship of age according to the teenagers' nature, there appeared some differences between the SP(Sensible Perceiving)type and the NT(iNtuitional Thinking)type(F=2.97, P<.05). And, in the intimacy degree of teacher, there appeared some differences between the SJ(Sensible Judging)type, the NT(iNtuitional Thinking)type, and the NF(iNtuitional Feeling)type and the SP(Sensible Perceiving)type(F=2.83, p<.05). Finally, at 16 Types according the MBTI test, there were some differences in the ESFJ group and the ISTP group recognizing the friendship of age (F=3.85, p<.001), and there was no difference in the intimacy degree of teacher.