Amiota okadai자연집단의 Isozyme 다형 현상에 관한 연구

이택준,송은숙 中央大學校 自然科學硏究所 1987 自然科學硏究所 論文集 Vol.1 No.-

The present study was carried out to investigate the isozyme polymorphism in natural populations of A. okadai collected from Mt. Sori and Mt. Juwang in Korea by means of starch gel and thin layered agar gel electrophoresis. The results obtained were as follows: 1. The natural populations of A. okadai from two local strains represented monomorphic in ACPH and GOT allels. On the other hand, polymorphic patterns were observed in α-GPDH, GDH, AO, and SDH, Est-α and Est -βisozymes. 2. In Mt. Sori population, the gene frequencies of F allele in α-GPDH, GDH, AO, and SDH were 0.4083, 0.4325,0.4803 and 0.3935, respectively. The gene frequencies of ??, ?? and ?? were 0.2023,0.6039 and 0.0376, respectively. In Mt. Juwang population, the gene frequencies of F alleles in α-GPDH, GDH, AO and SDH were 0.5306,0.5326,0.5185 and 0.4595, respectively. The gene frequencies of ??, ?? and ?? were 0.1728, 0.5864 and 0.2407, respectively. It showed that there were slight geographical differences in two populations, Mt. Sori and Mt. Juwang. 3. The average proportions of heterozygous individuals for α-GPDH, GDH, AO, SDH, ?? and Est-βin two populations were 0.5094, 0.5008,0.5118,0.5255 and 0.5626 ,respectively. 4. Seasonal wariation of Est-βallele was observed in Mt. Sori population from April to October. The results of homogeneity test represented significant differences between Spring and Summer populations, while Spring and Autumn populations showed some similarities in genetic constitution.

Drosophila auraria 自然集團의 酵素多型現像

徐珉錫,李澤俊 中央大學校 自然科學硏究所 1988 自然科學硏究所 論文集 Vol.2 No.-

Enzyme polymorphism in natural populations of Korean Drosophila auraria was analyzed. Electrophoretic techniques were emloyed to invesigate allelic variations at fifteen enzyme loci in eight natural populations. Eight loci (??, Me, Xdh, Odh, Adh, Ao, 6-Pgd, Fum) out of 15 loci were monomorphic with identical mobility in all populations and the other seven loci were polymorphic. Gluose-metabolizing enzymes such as ??, ??, Me, α-Gpd, 6-Pgd, Got and Fum were less genetically variable than non-glucose-metabolizeng enzymes (Acph, Aph, Adh, Est-α, Est-β, Odh, Ao, Xdh). At the ?? locus, three alleles were detected. The ?? allele was predominant in frequency, 0.9167 or more, and its average frequency was 0.9685. A similar gene frquency pattern was observed also for the α-Gpd and Got loci in all populations. The average proportion of heterozygous individuals was 0.0600, 0.0334 and 0.0730 for the Mdh^, α-Gpd and Got loci respectively. Six alleles were detected at the Acph locus in the present survey. The Acph⁴allele was the most frequent allele in six populations, ranging from 0.2472 to 0.4333, the averaige being 0.3350. The average proportion of heterozygous individuals at the Acph locus was 0.7684. At the Aph locus, four alleles were detected in eight populations. The most frequent allele was Aph³, ranging from 0.2429 to 0.6304, the average being 0.3975. The average proportion of heterozygous individuals was 0.6834 for the Aph locus. At the Est-α locus, eight alleles including a null allele were detected and the Est-α², α³,α⁴, ?? , and ?? alleles were detected in all populations. The frequency of allele ?? was considerably higher than those of the other alleles, being 0.4573 on the average. On the other hand. the α¹,α²and ?? alleles exist in low frequencies. The average proportion of heterozygous individuals for teh Est-αlocus was 0.7073. Five alleles including a null allele were found at the Est-β locus. Among them two alleles, Est-β²and β³, were frequent alleles in most populations studied. The most frequent allele was Est-β³, ranging from 0.4231 ot 0.5455, the average being 0.4848. The average polymorphism(%) and heterozygosity were 38.34 and 0.1395 respectively. The average genetic similarity between populations was 0.9623±0.01 and this value is as similar as other Drosophila studied. A comparison of genetic distances among eight populations estimated on the basis of the allelic frequency for fifteen enzyme loci surveyed showed little difference, giving an average distance of 0.0054±0.0025. It is considered that the reasons of less genetic variability in the natural populations of D. auraria may be due to genetic drift.

향류분배 장치에 의한 주광성 행동의 인위도태와 자연도태의 유전적 효과

추종길,한영주,임귀순 中央大學校 自然科學硏究所 1987 自然科學硏究所 論文集 Vol.1 No.-

Directional selections for positive and negative phototaxis were carried out with hte populations, derived from Jeju and L-F strain of Drosophila melanogaster, using the countercurrent distribution apparatus. Effects of selection for positive and negative phototaxis were clearly recognized in the early generations, and the populations were reached a selection plateau from the 5th generation onwards. After 9 generations of artificial selection, the populations selected for positive and negative phototaxis were relaxed in the following 16 generations. The phototactic responses to natural selection of positive and negative populations were not returned to their neutral states. As a result of artificial and natural selection, genes of phototactic behaviors measured by means of countercurrent distribution apparatus are possibly controlled by major genes, and they seem to be fixed in the early generations of artificial selection. Walking behavior in populations of flies with positive and negative phototactic respones at the 9th, 16th and 17th generations was measured by the connected test tube apparatus. The negative populations turned out slow walking behavior. It seems reasonable to assume that genes controlling negative phototaxis and slow walking behavior were accumulated and selected within populations simultaneously.

서울 南山의 植生

任良宰,朴裁泓,韓昌燮 中央大學校 自然科學硏究所 1987 自然科學硏究所 論文集 Vol.1 No.-

A study on the forest vegetation of Mt. Namsan, Seoul, was conducted from 1985 to 1986 for nature conservation. In the classification by Z-M method two plant communities(Quercus mongolica comm. and Pinus densiflora comm.) and four plantations with different species and one mixed (native and planted species) stand were recognized by the floristic composition obtatined from 18 Releve at random. By the results, the actual vegetation map and tree species distribution map of the mountain in scale 1 : 20,000 were prepared. The analysis of community structure and species diversity showed that the northern slope of the mountain is largely covered with relative stable communities while the southern slope with unstable communities. The forest vegetation destructed since the establishment of capital city in 1394, are moving now to the restoration courses under the legal supervision of Seoul city. However, an warning should be given to the undesirable plantation restricting forest succession suh as many sites at the southern slope.

배(Pear)중 Polyphenol oxidase에 관한 연구

조성희,황국섭 中央大學校 自然科學硏究所 1987 自然科學硏究所 論文集 Vol.1 No.-

Polyphenol oxidases were prepared from the various species of pears and observed their biochemical properties. The experimental results were summarized as follows: 1. The enzyme was active with o-diphenols and trihydroxy-phenols, but inactive toward m-diphenols, p-diphenols and monophenols. 2. The optimum pH was 6.5-7.0 3. The Km value of the enzymes were 25mM in Niidak, 31mM in Chojuro, and 67mM in Danbae with catechol as substrate. 4. The optimum temperature was 35℃. 5. Inhibitor studies indicated that sodium diethyldithio carbamate, L-cysteine, sodium bisulfite, L-ascorbic acid and 2-mercapto ethanol were the most potent, but NaCl and E.D.T.A. were impotent relatively. 6. ??, ??, ??, ?? and ??(1mM) activated the enzyme activity, but ?? and ??(1mM) inhibited partialy.

MNNG가 Proteus mirabilis균주의 치사율 항생제 내성 및 영양요구성에 미치는 효과

宋哲鏞,金星究 中央大學校 自然科學硏究所 1987 自然科學硏究所 論文集 Vol.1 No.-

It was known that the MNNG is one of the powerul chemical mutagens for many bacteria including E. coli and S. typhimurium. However, the effects of the MNNG on the mutant rate of Proteus mirabilis has not been elucidated. This study was undertaken to understand the mutation retes of the lethality and antibiotic resistance of streptomycin and auxotrophs of Proteus mirabilis treated with the MNNG. The lethal rates of P. mirabilis showed a peak as 50% in 50 minutes at a concentration of 150g MNNG per ml in nutrient broth. The higher MNNG oncentrations asd the longer times bacteria were treated with, the higher lethal rates were appeared. Antibiotic resistant mutants P. mirabilisl to streptomycin were observed in when treated with 50g concentration of MNNG, whereas such resistant mutant to tetracycline could not observed. Auxotrophic mutant rate of arginine and cysteine of P. mirabilis showed a dose-dependent against MNNG concentration, About 90% of arginine and 59.76% of cysteine prototrophs were transformed to auxotrophs at 100g and 150g MNNG, respectevely. Auxotrophic mutant rate of alanine and tryptophan of P. miarbilis showed a dose-independent against MNNG concentration. Both alanine and tryptophan auxotrophs were transformed to prototrophs at 50g MMNG.

마늘중 Peroxidas 의 정제 및 이의 특성에 관한 연구

조성희,차인돈 中央大學校 自然科學硏究所 1988 自然科學硏究所 論文集 Vol.2 No.-

Peroxidase was purified from garlic and observed its some biochemical properties. The experimental results were summarized as follows. 1. The peroxidase from garlic was purified about 32.4 fold by ammonium sulfate precipitation and DEAE-cellulose column chromagagraphy. 2. The more rapidly oxidized suvstrate was o-dianisidine, while benzine, guaiacol, and pyrogllol were oxidized with dereasing rates. But the oxidation of dihydroxyphenols and L-ascorbic acid were negligble. 3. The DTT and 2-mercaptoethanol have inhibited 100% of peroxidase activity at conentrations of 10mM and 1mM. but the peroxidase was slightly inhibited by EDTA and urea at the same concentrations. 4. The optimum temperature was 55℃ 5. The optimum pH was 5.6. 6. The Km values for hydrogen peroxide and guaiacol of purified peroxidase were 2mM and 0.9mM, respectively.

제주도에 있어서 내풍성정지전지(整枝剪枝)가 단감(Diospyros KaKi)의 결실율에 미치는 영향

이용수 中央大學校 自然科學硏究所 1988 自然科學硏究所 論文集 Vol.2 No.-

Wind protecting pruning test on the fruits set of Dyospyrus kaki L. This test had been carried out in the burricane problem area Jaeju Island during 1975 and 1976. Obtained result was remarkably good against the control plot for the fruit set.

무우종자의 발아촉진에 관한 연구

조성희,성찬기 中央大學校 自然科學硏究所 1987 自然科學硏究所 論文集 Vol.1 No.-

The changes of some components in the cell and germination rate of Raphonus Sativus L. seeds that were treated with GA₃, KINETIN and NAA were observed. The results were follows: 1. The content of soluble protein was increased in homogenate and cytosolic fractions of GA₃, and KINETIN treated seeds compared with one of control. 2. The content of glucose was increased in homoginate and cytosoli fractions of GA₃and KINETIN treated seeds compared with one of control on the half and the first day. 3. The activity of trehalase was increased in homogenate and cytosolic fractions of seeds which treated with GA₃and KINETIN compared with one of control on the first day. 4. The ATPase did not changed in all fractions of the untreated and stimulant treated seeds, during germination stages. 5. The germination rate was remarkedly increased by continuous soak treatment of GA₃and KINETIN on the first and second days but in the case of NAA treatment, it was very smiliar to control.

Pseudomonas aeruginosa 세포질내 단백질 분해효소의 특성에 관한 연구

宋哲鏞,李東皓 中央大學校 自然科學硏究所 1988 自然科學硏究所 論文集 Vol.2 No.-

A neutral protease was partially purified from a strain ATCC 25619-1 of Pseudomonas aeruginosa. The purification procedure included DEAE Trisacryl M ion exchange chromatography and AcA 54 gel giltration column chromatography. The partially purified enzyme had molecular weight of 51,000. This enzyme was actived against carbobenzoxy-arginyl-arginyl-7-amino-4-trifluoromethylcoumarin (CBZ-Phe-Arg-AFC). The optimal pH and molarity were pH 7.0 and 0.2M. The enzyme activity was inhited by leupeptin and diisopropyl phoshorofluoride (DFP) but not by other protease inhibitors, such as phenulmethylsulfonylfluoride(PMSF), N-ethyl-maleimide (NEM), pepstation-A, leupeptin, L-transepoxusuccinyl-leuchlamido (4-guanidino) butane (E-64), ethylenediamineteraacetate (EDTA), o-phenanthroline, and iodoacetic acid. These results indicated that the partally purified endoproteolytic enzyme' of a strain ATCC 25167-1 of P. aeruginosa was serine neutral protease.