In situ hybridization과 염색체 미세해부에 의한 특정 염색체 띠에 대한 유전자 발굴

홍기정,김현수,전용혁,김현,박선화 고려대학교 의과대학 1996 고려대 의대 잡지 Vol.33 No.1

Recently surmountable amounts of genes are being cloned but it is hard to define the chromosomal location and functions of these genes. In this study, a new technique was developed for cloning genes expressed in the developing human brain with confirmed chromosomal location. The mRNAs were extracted from 18 week old human fetal brain, cDNAs were made by reverse transcription from these mRNAs and Uni-amp cDNAs having Uni-amp adapters a t both ends were made for subsequent PCR. To observe the distribution of the Uni-amp cDNAs on the chromosome, fluorescent in situ hybridization was performed with biotin labeled Uni-amp cDNAs. Among the chromosome bands showing strong hybridization with the cDNAs, lq21, lq25, lq32 and lq42 were microdissected from the chromosome hybridized with unlabeled Uni-amp cDNAs and amplified by PCR with Uni-amp primers. These amplified cDNA fragments were subcloned to plasmid vectors and the nucleic acid sequences were analysed. As a result 18 different clones were confirmed the chromosomal localization. They were categorized as 4 clones of well characterized genes, 3 clones showing low homology with known genes, 5 clones of simply registered uncharacterized human cDNAs, and 6 clones of unknown genes. The evidence suggests that the technique is very useful to clone the genes that express in the developing human brain, with confirmed chromosomal location. In addition, this cloning technique can be used to discover the new genes related with neuronal development in combination with functional screening method.

THE KOMPSAT-I PAYLOADS OVERVIEW

Park, Hong Yul,Choi, Gi Hyuk,Yoon, Hyeong Sik,Lee, Seunghoon,Woo, Sun Hee,Shim, Hyung Sik,Oh, Kyoung Hwan,Cho, Young Min,Yong, Sang Soon,Lee, Sang Gyu,Heo, Haeng Pal 대한원격탐사학회 1998 International Symposium on Remote Sensing Vol.14 No.1

Korea Aerospace Research Institute (KAKI) is developing a Korea Multi-Purpose Satellite I (KOMPSAT-1) which accommodates Electro-Optical Camera (EOC), Ocean Scanning Multi-spectral Imager (OSMI), and Space Physics Sensor (SPS). The satellite has the weight of about 500kg and will be operated on the 10:50 AM sun-synchronized orbit with the altitude of 685 km. The satellite will be launched in 1999 and its lifetime is expected to be over 3 years. The main mission of EOC is the cartography to provide the images from a remote earth view for the production of 1/25000-scale maps of KOREA. EOC collects 510 - 730 nm panchromatic imagery with the ground sample distance(GSD) of 6.6 m and the swath width of 17 km by push broom scanning. EOC also can scan ±45 degree across the ground track using body pointing method. The primary mission of OSMI is worldwide ocean color monitoring for the study of biological oceanography. It will generate 6 band ocean color images with 800 km swath width and 1km GSD by whiskbroom scanning. OSMI is designed to provide on-orbit spectral band selectability in the spectral range from 400 nm to 900 nm through ground command. This flexibility in band selection can be used for various applications and will provide research opportunities to support the next generation sensor design. SPS consists of High Energy Particle Detector (HEPD) and Ionosphere Measurement Sensor (IMS). HEPD has missions to characterize the low altitude high-energy particle environment and to study the effects of radiation environment on microelectronics. IMS measures densities and temperature of electrons in the ionosphere and monitors the ionospheric irregularities at the KOMPSAT orbit.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

관동맥 재개통 후 QTc간격분산의 감소 : 정량적으로 측정한 심근 허혈의 호전 정도와의 연관성 Relationships with the Quantitative Degree of Improvement in Myocardial Ischemia

박상선,최기준,남기병,이철환,홍멸기,박성욱,박승정,신중우,문대혁,김유호 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.8

혈소판 감소증 환자에서 대마비와 전신 상태의 급격한 악화 : 급성 대동맥 혈전증 A Case Report of Acute Aortic Thrombosis 증례 보고

이홍기,박훤겸,백홍규,남영수,김영선,김용수 대한혈관외과학회 2002 Vascular Specialist International Vol.18 No.2

Acute aortic occlusion is rare but poses a high mortality and morbidity. Prompt diagnosis and treatment is essential. Typical presentation is rest pain with bilateral absent femoral pulse. When the non-typical symptoms predominate such as paraplegia, acute abdomen or sudden-onset hypertension, diagnosis may be difficult. We experienced a case of acute aortic thrombosis who suddenly developed paraplegia and rapidly deteriorated within several hours. When the paraplegia developed, he was under treatment with heparin and urokinase, was thrombocytopenic, and the femoral arteries were pulsatile. Spinal cord compression due to bleeding complication had to be ruled out. Duplex sonography and lumbar CT scan were not diagnostic. Aortic thrombosis was diagnosed by abdominal CT scan. This case illustrates the need for high suspicion of acute aortic occlusion presenting with paraplegia especially in patients with associated atherosclerotic disease.

한국 현대시 운율의 음향 발현

김현기,홍기환,김선숙 한국음성과학회 2004 음성과학 Vol.11 No.3

The metrical structures in orally produced the poetry were generally analyzed by accent, metre and syllable. The purpose of this study is to investigate of metrical structures of Korean modem poetry using computer implemented speech analysis system. Two famous poet's poems confidential talk, Miloe and 'A buddhist dance, Sungmu' were selected for prosodic analysis. The informant is 60 years old professor in major of Korean and French poetry. The syllable structures of poems were analyzed primarily by vowel timbers, which can classified compact and diffuse vowels according to the distance of F2-F1. The perception cues of consonants were analyzed by VOT and tensity features of articulation. Rhythm is classified by dactyl, anapest, trochee, spondee and iambic. As a result, syllable structures of Korean modem poetry were mainly CV and CVC and the reading times of each lines were 3-4sec for 12 and 15 syllables. Main metre of Korean modem poems constructed the Imbic and Anapest. The break of each lines were demarcated by grammatical structure or meaning rather than phonetic structures.

급성 ST 분절 상승 심근경색증의 표준진료지침 설계

권선옥,김우식,오명기,나종천,이홍기,조욱현,최석구 인제대학교 2006 仁濟醫學 Vol.27 No.-

The use of critical pathways for a variety of clinical conditions has grown rapidly in recent years, particularly pathways for patients with acute myocardial infarction. We intend to determine the impact of a clinical pathway on ST-elevation myocardial infarction (STEMI) and to evaluate the efficacy and safety of facilitated percutaneous coronary intervention (PCI) compared with primary PCI. Low risk STEMI patients (ST elevation >0.1mV in more than 2 limb leads or ST elevation >0.2mV in contiguous precordial lead, chest pain lasting more than 30 min without response to nitroglycerin) will be included. All patients will be also treated medically according to critical pathway. STEMI is one of the common diseases in emergency medicine and so it is necessary to establish realistic treatment guidelines. The use of critical pathways will improve the quality of care.

하천설계기준의 만곡부 수면형 결정 방법에 대한 적용성 검증

최한규,채홍기,백효선 강원대학교 산업기술연구소 2006 産業技術硏究 Vol.26 No.B

Through the result of calculating the deviation between the value calculated from two-dimensional number formula, one-dimensional number interpretation, and curving part water surface type calculation method, we could confirmed that the deviation is reduced more than 50% when we use curving part water surface type calculation method. Also it was confirmed that there occurs the reduction since the reduction rate of safe room height was 20% in 500 CMS of flood water quantity when we planed the construction of levee by curving part water surface type calculation method. And therefore, we have confirmed that the curving water surface type calculation method can be used as a simple formula in rivers with water quantity less than 500 CMS that flows in and out in less than 90 degree angle.

항결핵 치료과정에서 호전되었다가 악화된 뇌 결핵종 1예 보고

이대훈,유진홍,강미자,김홍기,김선화,고동훈,천지성,유원종 대한화학요법학회 1997 대한화학요법학회지 Vol.15 No.3

In spite of nationmide tuberculosis control program, tuberculosis is still prevalent in Korea. Accordingly, tuberculous meningitis also poses one of the main trouble some problems. Recently, We experienced a perplexing case of tuberculous menin-gitis and tubercdoma in a 24-year old female patient. She was admitted to our hospital owing to meningeal irritation sign, aphasia, VI nerve palsy, and ,fever. CSF analysis showed a finding consistent with that of presumptive tuberculous meningitis.. Initial MRI of the brain revealed a large mass(tuhercu1oma) on left parieto-temporal area. Antitubeculous regimen(1soniazid (INH), rifampin(RMP), ethambutol(EMB), pyrazinamide(PZA)} of meningeal dose was given immediately, and her symptoms and signs were dramatically disappeared with a few days after treatment. She was discharged with improved condition and under maintenance treatment of tuberculosis. Follow-up one year after treatment. revealed that the previous lesion was nearly diminished. She showed no symptom at all until she recognized a total blindness of sudden onset on her left eye a t the 18th month of treatment. Brain MRI checked at that time showed multiple masses of tuberculoma scattered over the brain base and one of them invaded left optic nerve. In our opinion, the cause of relapse in this patient might he explained by the selection and ultimate replication of a minor population of resistant strain. Although such relapse is an extremely rare event, we think that we should always pay attention to this possible resistance problem every time we meet patients with tuberculous meningitis.

폐혈관상이 유지된 선천성 낭성 선종양 기형

배일헌,한기석,차상훈,김성진,박길선,이건국,홍장수 충북대학교 의과대학 충북대학교 의학연구소 2003 忠北醫大學術誌 Vol.13 No.2

선천성 낭성 선종양 기형은 태생 16주 이후 말단세기관지의 형성이 중단되어 미성숙기관지들과 중배엽성분들이 과도하게 증식하여 발생하는 드문 선천성 폐질환이다. CT소견은 낭종의 크기 숫자, 내용물 등에 따라 다양하나, 병변내에 정상적인 폐혈관이 보인 예는 전세계적으로 보고된 적이 없었다. 저자들은 역동적 흉부 CT상 균일한 저음영의 병변내에 정상 폐혈관상이 유지된 선천성 낭성 선종양 기형 1예를 경험하였기에 보고한다. Congenital cystic adenomatoid malformation(CCAM) is a rare pulmonary disease that is thought to be developed by abnormal proliferation of immature alveoli and other mesenchymal component from the abrupt halting of the terminal alveoli formation after 16 weeks of gestation. On CT scan, there are no consistencies regarding to size, numbers, and its contents but no cases were reported in past showing normal pulmonary vascularities. We reports a case of CCAM that showed normal pulmonary vascularities within the hypodense lesions on a dynamic chest CT