正常韓國人의 血淸 Isocitric Dehydrogenase 活性値

全容赫 우석대학교 의과대학 1970 우석의대잡지 Vol.7 No.1

To evaluate normal value and range of serum isocitric dehydrogenase activity in Koreans, sera of the 50 apparently healthy Korean male adults were analysed and the results were as follows: Mean: 105±32 Sigma unit/ml with range of 55~175 Sigma units/ml which were not significantly different from those of Europeans.

正常韓國人의 血淸 Isocitric Dehydrogenase 活性値

全容赫 우석대학교 의과대학 1970 우석의대잡지 Vol.7 No.1

Vitamin E 投與마우스 飢餓에 對한 抵抗과 胃腸管粘膜에 미치는 影響에 關한 組織學的 硏究

全容赫 고려대학교 의과대학 1975 고려대 의대 잡지 Vol.12 No.3

To clarify the one of the physiological effects of vitamin E, 1976 adult albino mice were used in present study. Vitamin E was injected on a daily dose of 5㎎; 10 days for the adequate dose group and 20 days for the overdose group. After completion of injections, animals were put into the starvation-absolute and free access of water groups. Change of body weight, survival time withstanding to the starvation, histochemical observations of the gastrointestinal mucosa were calculated at the periods of starvation; 24, 48 hours and at the time of death. The results of the study are summarized as follows: 1. Administration of adequate dose of vitamin E resulted prolongation of survival time against starvation, but excess amount resulted shortening tendency in survival time. 2. Vitamine E administration accelerated the rate of body weight increment and the loss of body weight due to starvation was rather slow and moderate. 3. There was not much difference in histometrical feature of the gastrointestinal mucosa in adequate vitamin E administration group, but in histochemical finding, amount of cellular synthetic material was increased. However, overdose vitamin E group showed edematous mucous membrane and decreased stainability because of the reduction of the number of mucous secreting cells and cellular synthetic material. 4. The changes of histological finding due to starvation were more prominent in duodenal mucosa compared to that of stomach, but decrease in mucous material was more prominant in stomach. The neutral mucous substance in gastric mucosa, the acid mucous substance in duodenal crypt and the neutral mucus in duodenal villi were mainly decreased respectively. 5. Decrease of intensity and stain reactivity in secretory granules of Paneth cell due to starvation was suppressed by the vitamin E. It seems that vitamin E causes acceleration of the secretory granule synthesis. 6. Reduction in the number of epithelial lining cell of duodenum following starvation was more apparent in the villi than the crypt, while goblet cells decreased generally less grade. The tendency showed that vitamin E injected groups were suppressed in the cell reduction. 7. In general, free access of water groups survived longer than absolute starvation groups and showed mild reduction whenever fasting proceed. However, any noticeable histological difference was not found between two groups.

四肢發生 過程에 關한 觀察

全容赫,朴善華 고려대학교 의과대학 1992 고려대 의대 잡지 Vol.29 No.2

Developmental sequence of events of fore-limb in DDY mouse embryo were observed to obtain the data on shape ; on histology with special reference to the apical ectodermal ridge(AER), programmed cell death(PCD) and chondro-osteogenesis and ; on changes of glycoconjugates. Fore-limb buds appeared between the day 9.5 and 10 of gestation(plug day=0) and distal ends grew into paddle-like plates from day 11, plates condensed to form digital rays on day 12, webbed fingers on day 13 and fingers were separated on day 14. Well developed AER appeared with the beginning of elevation of buds and decreased in size from day 11 and remained remnants on day 12.5 of gestation. Proximal core mesenchyme began to condense form day 11 and transformed to precartilaginous zone 12 hours later chondrified from day 12.5 and ossified from day 15 and these phenomena proceeded proximo-distal sequence. Programmed cell death happened only between the day 10.5 to 11.5 and were confined to the proximal and middle zone of mesenchyme, which comes into question how PCD In motioned area and time correlate with subsequent morphogenses. PAS positive reactivity was gradually increased in the epithelium. AER and cartilaginous zone with the development proceed but that of mesenchyme was weak throughout the period. and in comparison with PAS reactivity, PAPS reactivity to the epithelium was always less but no difference in cartilaginous zone which suggest that amount and component of glycoeonjugates are variable and tissue/stage-specific in the developing limb.

畸形原的 危害性 判別을 위한 Hydra Attenuata System의 評價

全容赫 고려대학교 의과대학 1983 고려대 의대 잡지 Vol.20 No.2

The multiplicity of potential teratogenic mechanisms requires that a rapid and practical screening system for adverse effects on development must be able to assess not just one or two developmental events but as much of the entire phenomena as possible because it is not yet possible to explain the mechanism on triggering event for and teratogenic outcome. Artificial hydra "embryo" is obviously quite different from a real embryo on first inspection. On closer examination, however, it seems to share a great many developmental capabilities with any ontogenic system. Sequence of events the "embryo" undergoes during reaggregation and regeneration, such as spatical orientation, cell sorting, recognition of neighbors and formation of cell junction, formationa of extracellular material, tissue organization, response to induction and formation of organ fields all appear to be similar to those events required during mammalian embryogenesis. In addition, the "embryo" 1) has an obvious and complex ontogeny, 2) reacts to a teratogen in a dose=related manner, 3) has an evident threshold effect to the drug, 4) illustrate that developmental critical periods exist and that the developmental stage at the time of insult is more important perhaps than the severity or nature of the insult, 5) reacts in a somewhat stereotyped manner and 6) the system itself, also can provide the two relevant dose levels based on toxicity as adequately as standard laboratory rodents but at a significantly lower cost in time and effort.

發生中인 口蓋組織에서 Collagen 및 HSPG의 分布와 口蓋裂유발인자가 이에 미치는 영향에 관한 관찰

全容赫,羅福瑛 고려대학교 의과대학 1988 고려대 의대 잡지 Vol.25 No.2

Fluorescence immunohistocheulstry using collagen type Ⅲ, Ⅳ and heparan sulfate proteoglycan(HSPG)-specific antibodies was performed in the developing secondary palate of the DDY mouse and during the process of teratogen-induced cleft palate. Immunostaining with antibody to type Ⅲ collagen was present and localized in the basement membrane from the begining of formation of palatal shelf but it appeared in mesenchyme beneath the basement membrane during the mid-maturation period of the shelf and intensity of staining in mesenchyme was decreased prior to shelf elevation and disappeared both from basement membrane and mesenchyme with the shelf elevation: while triamcinolone and isotretinoin accelerated its decrement from both area. Type Ⅳ collagen and HSPG were demonstrated and localized in the basement membrane from the begining of shelf formation until its maturation and the fluorescence to type Ⅲ, Ⅳ and HSPG were not observed in epithelia during all stages of development. The results suggest that type Ⅲ and Ⅳ collagen involves in initiation and early maturation of palatal shelf but not in late maturation, elevation and fusion of the shelf.

마우스 및 鷄 胎齡에 따른 骨·軟骨의 二重染色法

전용혁,박선화,서영석 고려대학교 의과대학 1984 고려대 의대 잡지 Vol.21 No.2

Owing mainly to differences in race and fetal age, the procedures for dual staining of cartilage and bone in rodents, which have been used, cause a little trouble in applying then to the developing skeleton, Here, we present a simple and rapid procedure, a modification of previous ones, for staining of fetal skeletal from its formation until birth in mice and chick that are frequently used in teratology research. The procedure and results are summarized as follows; 1. Complete skinning and evisceration contributed to the whole procedure which yields high quality results. 2. Skinned and eviscerated specimen was stained and fixed in a mixture of 0.15% alizarin red S and 0.14% Alcian blue in ethanol and glacial acetic acid for 10 to 12 hours for cartilaginous skeleton but 15 to 20 hours for ossified one in both animals. 3. In time for tissue maceration; the longer, the better but it might separate the skeleton from the joint. So, time for it was prolonged stepwise from one hour to 2, 3, 4, 6 and 8 hours in 2% KOH solution for the fetus at 14th, 15th, 16th, 17th, 18th and 19th day of gestation respectively in mice; and in chick, 30 minutes, 1, 2 and 3 hours in 1% KOH solution for the fetus aged 6, 7, 8 and 9 day and 2, 3, 4, 5, 6, 8 and 10 hours in 2% solution for 10, 11, 12, 13, 14, 15 and over 16 day respectively. 4. Subsequently, specimens in both animals were placed in 1 : 1 distilled water and glycerin for 12 to 14 hours then in 1 : 3 solution for 24 hours for clearing and hardening prior to final storage in pure glycerin. High quality fetal specimens can be prepared for examination by this procedure within 30 hours and preserved for a long time.

Vitamin E 投與마우스 飢餓에 對한 抵抗과 胃腸管粘膜에 미치는 影響에 關한 組織學的 硏究

全容赫 고려대학교 의과대학 1975 고려대학교 의과대학 잡지 Vol.12 No.3

Vitamin E 投與가 마우스 諸組織에 미치는 影響에 關한 實驗的 硏究 : Ⅰ. 骨格筋纖維에 關한 組織學的 觀察

全容赫 고려대학교 의과대학 1973 고려대 의대 잡지 Vol.10 No.2

Changes of muscle fiber size that follow vitamin E administration and vitamin E deficiency in the mouse were observed. Mean diameter and percentile size distribution were calculated in the sartorius and back muscles. Of sartorius muscle, the mean diameter was measured as 36.0 microns and their size distribution extends from 10 microns to 70 microns in normal control group. Of back muscle, mean diameter was 35.5 microns and range also between 10 to 70 microns. Vitamin E administration resulted slight increase in the mean diameter of fibers, while a deficiency caused positive decrease and irregularity in size distribution in both muscles.

Vitamin E 投與가 마우스 諸組織에 미치는 影響에 關한 實驗的 硏究

全容赫 고려대학교 의과대학 1973 고려대학교 의과대학 잡지 Vol.10 No.2